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Biodiversitas Journal of Biological Diversity
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Articles 6,259 Documents
Optimizing extraction timing and characterizing the sex pheromone profile of the fall armyworm (Spodoptera frugiperda) in Indonesia VANI NUR OKTAVIANY SUBAGYO; RAFIKA YUNIAWATI; PURNAMA HIDAYAT; DADANG DADANG; HERY WIDYANTO; I MADE SAMUDRA
Biodiversitas Journal of Biological Diversity Vol. 27 No. 2 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270238

Abstract

Abstract. Subagyo VNO, Yuniawati R, Hidayat P, Dadang, Widyanto H, Samudra IM. 2026. Optimizing extraction timing and characterizing the sex pheromone profile of the fall armyworm (Spodoptera frugiperda) in Indonesia. Biodiversitas 27 (2): d270238. https://doi.org/10.13057/biodiv/d270238. The fall armyworm (Spodoptera frugiperda) threatens maize production in Indonesia, highlighting the need for pheromone tools tuned to local populations. We defined an operational extraction window and quantified the gland pheromone profile of Indonesian FAW females as a baseline for lure development. Virgin females from nine populations were sampled across age (1-4 days) and time after scotophase onset (2-6 hours). Pheromone glands were extracted in hexane and analyzed by GC-FID for three acetate components (Z7-12:OAc, Z9-14:OAc, Z11-16:OAc). In total, 130 extract vials (286 females pooled at 1-3 per vial) were analyzed; the vial was treated as the experimental unit and amounts were expressed as ng per gland. Total pheromone yield showed a consistent mid-scotophase ridge (generally 3-6 hours after lights-off), most apparent in 2-3-day-old females. A linear mixed model on log10(Total_ng) with Population as a random intercept detected a significant Age×Hour interaction (LRT χ²: 19.176, df: 10, p: 0.0381). Cells were ranked by mean Total_ng while prioritizing replication (n_vials ≥3) and population coverage; the selected global window was 3 days, 5 hours. Within this window, composition was consistently Z9-major (mean±SD: 80.18±13.61% Z9-14:OAc; 16.15±13.44% Z11-16:OAc; 3.67±5.31% Z7-12:OAc; n: 28 vials). These results provide a reproducible extraction standard and a locally derived reference blend (≈80:16:4) for subsequent behavioral assays and multi-site field validation.
Avian community composition and feeding guilds in the agricultural area of Padangsidimpuan City, North Sumatra, Indonesia NURUL HUSNA SIREGAR; RIZKY AMELIA DONA SIREGAR; PERIMA SIMBOLON
Biodiversitas Journal of Biological Diversity Vol. 27 No. 5 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270524

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Abstract. Siregar NH, Siregar RAD, Simbolon P. 2026. Avian community composition and feeding guilds in the agricultural area of Padangsidimpuan City, North Sumatra, Indonesia. Biodiversitas 27 (5): d270524. https://doi.org/10.13057/biodiv/d270524. Agricultural expansion has altered tropical landscapes and may influence bird communities. This study aimed to examine bird community composition, feeding guild structure, and conservation implications in agricultural areas of Padangsidimpuan City, North Sumatra, Indonesia. Data were collected from August to September 2025 using point count surveys at three sites selected based on dominant agricultural land use and environmental variation, particularly elevation and vegetation differences. Each site included five observation points spaced 200 m apart, totaling 45 observation events with three repeated surveys per point. A total of 907 individual birds were recorded, representing 60 species from 30 families. The highest species richness and diversity were recorded in Aek Najaji (diversity index: 2.87; richness index: 6.30), followed by Bonan Dolok and Lubuk Raya. Differences among sites indicate variation in species composition and abundance despite similar land-use types. Nine feeding guilds were identified, with insectivores representing the highest number of species (17 species), followed by frugivore-insectivores (13 species). Higher diversity observed at sites with more heterogeneous vegetation and topography suggests a potential association between habitat complexity and bird communities, although habitat variables were not quantitatively analyzed. These findings indicate that agricultural landscapes may contribute to supporting bird diversity. Management practices such as maintaining mixed garden systems, preserving natural vegetation, and retaining riparian elements may support the persistence of bird communities in agricultural landscapes.
Ecological and taxonomic diversity of nematode communities in soils of the Zarafshan Valley (Uzbekistan) and its edaphic determinants DILNOZA M. NURMATOVA; SARDORBEK B. NARZULLAYEV; OCHIL MAVLYANOV; UKTAM N. MIRZAEV; RUSTAM SH. URALOV; SHAHLO SH. TURSUNOVA; FERUZ A. SHODMONOV; JAKHONGIR E. OBLOKULOV; SARDORBEK S. KAMBAROV; INTIZOR R. AVAZMETOVA; KHULKAR SH. URINOVA; DILFUZA SH. YODGOROVA
Biodiversitas Journal of Biological Diversity Vol. 27 No. 2 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270203

Abstract

Abstract. Nurmatova DM, Narzullayev SB, Mavlyanov O, Mirzaev UN, Uralov RS, Tursunova SS, Shodmonov FA, Oblokulov JE, Kambarov SS, Avazmetova IR, Urinova KS, Yodgorova DS. 2026. Ecological and taxonomic diversity of nematode communities in soils of the Zarafshan Valley (Uzbekistan) and its edaphic determinants. Biodiversitas 27 (2): d270203. https://doi.org/10.13057/biodiv/d270203. Soil nematodes are key bioindicators of belowground ecosystem functioning and soil health. This study analyzed the trophic and taxonomic structure of nematode communities in apple orchards of the Zarafshan Valley (Uzbekistan) and assessed their relationships with soil physicochemical properties. The study, conducted from 2023 to 2025, investigated the nematode diversity associated with intensive apple orchards in depth, in relation to soil factors. A total of 240 samples were collected from 8 apple orchards using the route method in spring, summer, and fall. The samples were processed using the modified Berman’s funnel method and flotation method. A total of 1277 individuals representing 54 nematode species, 25 genera, and four orders were identified from root and soil samples collected at 0-20 and 20-40 cm depths. The highest diversity of species and individuals was observed in the top layer of soil. This layer contained 90% of the species and 59% of the individuals of the fauna. The dominant ecological groups were omnivores-predators (Dorylaimus, Eudorylaimus), bacterivores (Cephalobus, Panagrolaimus), and plant parasites (Pratylenchus, Helicotylenchus). Species richness and abundance were markedly higher in the upper soil layer, which contained more humus, nitrogen, and phosphorus, and had lower bulk density. Principal Component Analysis (PCA) revealed that humus, N, P, pH, and K were the main environmental determinants shaping nematode community composition. According to PCA analysis, bacterivores and omnivores had positive correlations with humus and N, while plant parasites were mainly associated with high P and K concentrations. These findings highlight the ecological sensitivity of nematode trophic groups to soil fertility gradients and physicochemical conditions. By studying the taxonomic and ecological diversity of nematodes, revealing their degree of association with soil factors, it is possible to improve intervention strategies to maintain soil health and stability in semi-arid horticultural ecosystems.
First molecular records of bacteria infecting bagworms in oil palm plantations of South Sumatra, Indonesia ERISE ANGGRAINI; EVI RIZKYANI NASUTION; SITI HERLINDA; CHANDRA IRSAN; AHMAD MUSLIM; SUWANDI SUWANDI; NURHAYATI DAMIRI; WEI HONG LAU
Biodiversitas Journal of Biological Diversity Vol. 27 No. 1 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270118

Abstract

Abstract. Anggraini E, Nasution ER, Herlinda S, Irsan C, Muslim A, Suwandi S, Damiri N, Lau WH. 2026. First molecular records of bacteria infecting bagworms in oil palm plantations of South Sumatra, Indonesia. Biodiversitas 26 (1): d270118. https://doi.org/10.13057/biodiv/d270118. Bagworms such as Metisa plana, Pteroma pendula, and Mahasena corbetti are major defoliating pests in oil palm ecosystems and can cause substantial yield losses during outbreaks. Despite their economic importance, information on bacteria infecting these bagworm species in Indonesia remains limited. This study presents the first molecular identification of bacterial isolates obtained from three dominant bagworm species collected in Banyuasin, South Sumatra, Indonesia. Partial 16S rRNA gene sequences were analyzed using BLASTn and Bayesian phylogenetic inference to determine their taxonomic affiliations. The isolate from P. pendula was identified as Stenotrophomonas maltophilia, forming a well-supported clade with reference sequences in the Bayesian phylogenetic tree. The isolate from M. plana showed high sequence similarity to Bacillus thuringiensis, while the isolate from M. corbetti was identified as Mammaliicoccus sciuri and clustered distinctly from Staphylococcus species. All sequences were deposited in GenBank under accession numbers PX487709.1, PX487712.1, and PX487708.1, respectively. These findings represent the first molecular records of bacteria infecting oil palm bagworms in South Sumatra and demonstrate that different bagworm species can be infected by distinct bacterial taxa. A laboratory bioassay using third-instar Spodoptera litura larvae was conducted as a preliminary assessment of biological activity, indicating variable larval responses among the bacterial isolates. While B. thuringiensis remains the only well-established entomopathogenic species, the responses observed for S. maltophilia and M. sciuri suggest that their interactions with insect hosts merit further investigation. Overall, this study provides baseline molecular evidence of bacterial infections in oil palm bagworms and contributes to a broader understanding of insect-microbe diversity in plantation ecosystems.
Quantitative ethnobotanical assessment of medicinal plants in Zagatala District, Azerbaijan JAVANSHIR ISA ISAYEV; GUNAY SALEH JAFAROVA; NILUFAR MUBARIZ SAFAROVA; ESMIRA MIRBABA HAJIYEVA
Biodiversitas Journal of Biological Diversity Vol. 27 No. 3 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270309

Abstract

Abstract. Isayev JI, Jafarova GS, Safarova NM, Hajiyeva EM. 2026. Quantitative ethnobotanical assessment of medicinal plants in Zagatala District, Azerbaijan. Biodiversitas 27 (3): d270309. https://doi.org/10.13057/biodiv/d270309. This cross-sectional ethnobotanical study, conducted between 2023 and 2025, provides a quantitative assessment of medicinal plants traditionally used by the multi-ethnic communities of the Zagatala District in northwestern Azerbaijan. Ethnomedicinal knowledge was documented through structured and semi-structured interviews with 105 informants, recording 121 medicinal plant taxa belonging to 115 genera and 44 families. Quantitative ethnobotanical indices - including Use Value, Relative Frequency of Citation, Fidelity Level, Family Importance Value, and Informant Consensus Factor (UV, RFC, FL, FIV, and ICF) - were applied to assess cultural significance and knowledge consensus. The highest UV values were recorded for Crataegus orientalis (0.76), followed by Thymus caucasicus (0.74) and Cydonia oblonga (0.73). Species with high RFC values, such as Rosa tomentosa (RFC = 0.96), T. caucasicus (0.96), and Alcea rugosa (0.94), were the most frequently cited by informants. ICF values were high across all ailment categories (0.94-1.00), with particularly strong consensus for laxative uses (ICF = 0.98), cardiovascular disorders (0.98), and broncholytic uses (0.99). These findings document the rich ethnomedicinal heritage of the region and identify C. orientalis, T. caucasicus, and C. oblonga as culturally prominent species that merit further phytochemical and pharmacological investigation, while emphasizing the importance of preserving traditional knowledge and supporting sustainable management of local plant diversity. This study provides a quantitative baseline for future pharmacological validation, conservation planning, and sustainable management of medicinal plant resources in Azerbaijan and the wider Caucasus region.
Exploration of bacterial communities for low-density polyethylene (LDPE) degradation in the mangrove area of Pamekasan, East Java, Indonesia JUALITA FARADHA ACHNASYA; ARTINI PANGASTUTI; SITI LUSI ARUM SARI
Biodiversitas Journal of Biological Diversity Vol. 27 No. 3 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270325

Abstract

Abstract. Achnasya JF, Pangastuti A, Sari SLA. 2026. Exploration of bacterial communities for low-density polyethylene (LDPE) degradation in the mangrove area of Pamekasan, East Java, Indonesia. Biodiversitas 27 (3): d270325. https://doi.org/10.13057/biodiv/d270325. Low-density polyethylene (LDPE) is one of the most widely used synthetic plastics and a major contributor to persistent pollution in coastal ecosystems, including mangrove environments. Mangroves act as natural sinks for plastic debris, yet the diversity and functional potential of plastisphere-associated bacteria in these systems remain insufficiently understood. This study investigated bacterial community dynamics during LDPE-driven enrichment and identified indigenous LDPE-degrading bacteria from mangrove sediments in Pamekasan, East Java, Indonesia. Sediment samples from three mangrove sites were subjected to three sequential enrichment cultures using carbon-free marine medium with LDPE beads as the sole carbon source. Microbial community shifts were analyzed through metagenomic sequencing, while LDPE degradation capacity was evaluated using gravimetric weight loss, FTIR spectroscopy, and SEM-EDX analyses. Enrichment resulted in pronounced restructuring of bacterial communities, characterized by reduced richness but increased functional specialization across culture stages. Dominant taxa included Qipengyuania, Flavobacterium, Acinetobacter, Pseudoxanthomonas, and Pseudomonas, with a stable core of ASVs persisting throughout enrichment. From the tertiary culture, three culturable isolates demonstrated LDPE-degrading potential: Microbacterium aurantiacum K2, Microbacterium sp. K3, and Bacillus sp. K5. Among these, Bacillus sp. K5 exhibited the highest degradation efficiency, achieving 21.35% LDPE weight loss, followed by Microbacterium sp. K3 (20.27%) and M. aurantiacum K2 (17.10%). FTIR and SEM analyses confirmed oxidative surface modification and structural damage of LDPE films. These findings demonstrate that mangrove plastisphere communities harbor specialized bacteria capable of initiating LDPE degradation and highlight the ecological role of enrichment-driven microbial selection. The study provides a foundation for developing nature-based and environmentally compatible strategies for mitigating plastic pollution in coastal ecosystems.
Molecular identification of indigenous arbuscular mycorrhizal fungi in the rhizosphere of black pepper (Piper nigrum) in West Lampung, Indonesia RAHMAT HIDAYAT; MARIA VIVA RINI; RUSDI EVIZAL; AGUS KARYANTO; PAUL BENYAMIN TIMOTIWU; SALWA AZZAHRA
Biodiversitas Journal of Biological Diversity Vol. 27 No. 5 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270526

Abstract

Abstract. Hidayat R, Rini MV, Evizal R, Karyanto A, Timotiwu PB, Azzahra S. 2026. Molecular identification of indigenous arbuscular mycorrhizal fungi in the rhizosphere of black pepper (Piper nigrum) in West Lampung, Indonesia. Biodiversitas 27 (5): d270526. https://doi.org/10.13057/biodiv/d270526. Black pepper (Piper nigrum) serves as a vital economic spice commodity in Indonesia, yet its agricultural productivity is frequently constrained by suboptimal soil conditions, particularly in acidic marginal drylands. The use of indigenous Arbuscular Mycorrhizal Fungi (AMF) offers a sustainable, environmentally friendly strategy to mitigate these abiotic stresses. However, the specific diversity of AMF communities in the black pepper plantations of West Lampung remains largely underexplored. Therefore, this study aimed to conduct a species-level molecular identification of indigenous AMF isolated from the black pepper rhizosphere in this region. Five distinct spore isolates were successfully recovered using a trap culture method and were initially characterized based on morphological attributes. For accurate taxonomic resolution, molecular analysis was performed using nested Polymerase Chain Reaction (PCR) targeting the 18S Small Subunit (SSU) rRNA gene with AML1/AML2 primers. This was followed by DNA sequencing and phylogenetic reconstruction using the Neighbor-Joining method. The phylogenetic analysis confirmed the presence of four distinct evolutionary lineages: Ambispora leptoticha (SP1), Acaulospora cf. spinosa (SP2), Acaulospora colombiana (SP4), and unclassified Glomeromycotina sp. (SP3 and SP5). Interestingly, the typically ubiquitous genus Glomus was entirely absent from the analyzed isolates, while the sampled soil exhibited extreme acidity (pH 4.13) and unexpectedly high available phosphorus (77.17 ppm). This study provides a crucial molecular baseline for indigenous AMF, confirms the first molecular record of the Ambispora lineage, and supports the future development of site-specific bioinoculants tailored for acidic soils.
Vegetation structure, microclimate, and edaphic variables as determinants of macrofungi occurrence in the Maros-Pangkep Karst Area, Indonesia ALI HASAN SALMAN; MUHAMMAD RESTU; SITI HALIMAH LAREKENG; RETNO PRAYUDYANINGSIH
Biodiversitas Journal of Biological Diversity Vol. 27 No. 3 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270303

Abstract

Abstract. Salman AH, Restu M, Larekeng SH, Pryudyaningsih R. 2026. Vegetation structure, microclimate, and edaphic variables as determinants of macrofungi occurrence in the Maros-Pangkep Karst Area, Indonesia. Biodiversitas 27 (3): d270303. https://doi.org/10.13057/biodiv/d270303. The Maros-Pangkep karst region in South Sulawesi, Indonesia, is known as the second largest karst in the world with a unique tower karst landscape and high biodiversity, including macrofungi. This study aimed to analyze the relationship between the presence of macrofungi and edaphic, microclimate, and vegetation factors to deepen the understanding of fungal ecology. Field surveys were conducted across 15 sampling plots representing different vegetation conditions. Macrofungi composition was recorded alongside measurements of microclimate variables (air temperature, humidity, light intensity) and edaphic properties (soil moisture, pH, organic carbon, total nitrogen, and available phosphorus). A total of 52 macrofungi species, belonging to 35 genera and 24 families, were documented, with Polyporaceae as the most dominant family. Species richness and diversity indices varied among plots, reflecting differences in vegetation complexity and environmental conditions. Principal Component Analysis (PCA) indicated that air humidity and soil moisture were the strongest variables associated with macrofungi occurrence, while higher temperature and light intensity showed negative associations. Edaphic factors, particularly soil moisture and organic carbon, contributed more strongly to macrofungi abundance than soil nutrient concentrations. Vegetation structure, especially sapling density and herbaceous cover, was associated with macrofungi abundance, whereas tree density was more closely related to species richness. These results demonstrate that macrofungi occurrence in karst ecosystems is shaped by the interaction of microclimatic conditions, soil properties, and vegetation structure rather than by single environmental drivers. The study highlights the importance of maintaining humid microhabitats and heterogeneous vegetation to support macrofungi diversity in karst landscapes. These findings provide a deeper understanding of macrofungi ecology and serve as an important basis for identifying macrofungi species with potential for utilization in various fields.
Environmental distribution patterns and sustainability assessment of mitre squid Uroteuthis chinensis in the waters of Bangka, Indonesia VALENTIN VINA RATNAPURI; MONISCHA BR SEBAYANG; MUHAMAD FATHUR ROZAKI
Biodiversitas Journal of Biological Diversity Vol. 27 No. 5 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270517

Abstract

Abstract. Ratnapuri VV, Sebayang MB, Rozaki MF. 2026. Environmental distribution patterns and sustainability assessment of mitre squid Uroteuthis chinensis in the waters of Bangka, Indonesia. Biodiversitas 27 (5): d270517. https://doi.org/10.13057/biodiv/d270517. The waters of Bangka were among the main mitre squid Uroteuthis chinensis production areas in Indonesia, but they showed signs of increased exploitation pressure that could affect stock sustainability. This study aimed to integrate the satellite-based Environmental Suitability Index and Maximum Sustainable Yield for mitre squid in Bangka, with a monthly distribution resolution that explicitly distinguished between productive and non-productive seasons. Satellite imagery data and catch landings were used for the period September 2024-August 2025, while the Maximum Sustainable Yield was estimated for the period 2020-2024. The weighting of environmental parameters in the Environmental Suitability Index (ESI) was conducted through Principal Component Analysis and Pearson Correlation. The results showed that the Environmental Suitability Index value had a strong and significant positive relationship with CPUE (r: 0.838; p: 0.001; R²: 0.6503), with periods of moderate to high ESI values (≥0.4) being associated with increased CPUE (0.539-0.755 ton/trip), while low ESI values (<0.4) were associated with low CPUE (0.017-0.552 ton/trip). On the other hand, sea surface temperature (29.558-30.848°C) and chlorophyll-a (0.279-0.375 mg m-³) did not show a significant linear relationship with CPUE, thus were interpreted as indicators of habitat conditions rather than direct drivers of productivity. The Maximum Sustainable Yield analysis showed that in 2023, production reached 9,157 tons, exceeding the Maximum Sustainable Yield estimate of 7,865 tons (16.43%), with an effort level of 65,513 trips, surpassing the Fopt of 35,800 trips (82.72%), thereby indicating potential fishing pressure. Overall, these findings affirmed that the multivariate approach based on the Environmental Suitability Index was more effective in representing habitat suitability and highlighted the importance of seasonally adaptive management. However, the interpretation of results and management recommendations in this study required further validation through long-term data and scenario-based implementation before operational application.
Biodiversity and safety profiling of indigenous Enterococcus faecalis from Nem Chua, a traditional Vietnamese fermented pork ecosystem NGUYEN PHUONG THUY; NGUYEN VIET KHANH HUNG; VO NGUYEN NGOC NHU
Biodiversitas Journal of Biological Diversity Vol. 27 No. 1 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270136

Abstract

Abstract. Thuy NP, Hung NVK, Nhu VNN. 2026. Biodiversity and safety profiling of indigenous Enterococcus faecalis from Nem Chua, a traditional Vietnamese fermented pork ecosystem. Biodiversitas 27 (1): d270136. https://doi.org/10.13057/biodiv/d270136. Traditional Vietnamese fermented pork (Nem Chua) represents a specialized ecological niche, yet the microbial biodiversity and safety profiles of its non-conventional Lactic Acid Bacteria (LAB) remain insufficiently characterized for functional applications. This study explored the microbial reservoir of Nem Chua to isolate and evaluate indigenous LAB with biotechnological potential. Through a systematic multi-stage screening pipeline of 50 artisanal samples, an initial pool of 59 isolates was narrowed to 26 candidates based on functional and safety filters. Enterococcus faecalis NC5 was ultimately selected as the optimal candidate due to its superior ecological fitness and validated safety status. Molecular identification confirmed 100% identity with E. faecalis. NC5 demonstrated exceptional gastrointestinal resilience, maintaining viability of 4.32±0.05 Log CFU/mL at pH 2.0 and 3.82±0.02 Log CFU/mL in 1.0% bile salts. Furthermore, it exhibited potent broad-spectrum antagonism against Escherichia coli, Salmonella typhimurium, and Staphylococcus aureus. Safety profiling confirmed γ-hemolytic activity, suggesting the strain’s potential as a protective starter culture. Molecular identification based on partial 16S rRNA gene sequencing confirmed NC5 as E. faecalis with 100% sequence similarity to reference strains in GenBank. These findings suggest that E. faecalis NC5 is a robust and safe autochthonous component of the natural Nem Chua microbiota. This research highlights the vital role of preserving fermented-food biodiversity to enhance the safety and functionality of traditional Southeast Asian food systems.

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