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Nusantara Bioscience
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 545 Documents
Species diversity, biological spectrum and phenological behavior of vegetation of a Muslim sacred grove in Southwest Bengal, India Dr. Uday Kumar Sen Uday; RAM KUMAR BHAKAT
Nusantara Bioscience Vol. 13 No. 2 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130212

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Abstract. Sen UK, Bhakat RK. 2021. Species diversity, biological spectrum and phenological behavior of vegetation of a Muslim sacred grove in Southwest Bengal, India. Nusantara Bioscience 13: 219-231. Sacred groves are an age-old and worldwide phenomenon. They are traditionally made up of forest or surrounding forest zones that local people safeguard based on their spiritual relationship with the deities or ancestral spirits believed to reside in them. Social practices play a crucial role in conserving the environment and biodiversity. This research aimed to examine the current flora of a sacred grove's plant resources, biological spectrum, leaf size spectra, conservation status, and management of the Faringdanga Pir Babar Asthana in the Paschim Medinipur District of West Bengal in India. The floristic element of the study area revealed that the vegetation was diverse and composed of 162 species belonging to 148 genera over 51 families under 26 orders according to the Angiosperm Phylogeny Group (APG) IV classification. Because the study area is a sacred grove, it is relatively undisturbed, and traditional beliefs ensure that germplasm in the grove is protected. The current study could serve as a model for phytoclimatic research in India's sacred forests.
Gas Chromatography-Mass Spectrometry profiling, and evaluation of antioxidant and antibacterial activity of Albizia spp. Arindam Ghosh; SOUMYA MAJUMDER; SUMEDHA SAHA; SAHADEB SARKAR; MALAY BHATTACHARYA
Nusantara Bioscience Vol. 13 No. 2 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130207

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Abstract. Ghosh A, Majumder S, Saha S, Sarkar S, Bhattacharya M. 2021. Gas Chromatography-Mass Spectrometry profiling and evaluation of antioxidant and antibacterial activity of Albizia spp. Nusantara Bioscience 13: 177-184. Plant resources have been utilized for human welfare since ancient times. Most of the pharmaceutical industry relies on bioactive molecules from plant resources. This research was carried out to investigate the antioxidant and antibacterial activities of Albizia spp. DPPH assay was conducted to investigate the antioxidant activity, and a well-diffusion test by pour plate method was followed for antibacterial assay. Antioxidant activity confirmed that the leaf extracts with chemical contents having solubility in polar solvents showed high free radical scavenging activity. Bark extracts' free radical scavenging activities were lower than the leaf extracts. Leaf extracts of Albizia procera showed the highest scavenging activity (94.77% or 100.66 µg AE/ ml), followed by Albizia odoratissima (93.52% or 99.23µg AE/ ml), Albizia lebbeck (91.68% or 97.13 µg AE/ ml) and Albizia chinensis (89.91% or 95.11 µg AE/ ml). GC-MS analysis of leaf and bark extracts revealed that 15 compounds had been reported to have antioxidant activities such as beta-amyrin, phytol, squalene, and vitamin E, and 31 compounds had been reported to have antimicrobial, antibacterial, antifungal, and antiviral activity. The antibacterial activities of leaf and bark extracts of four Albizia species showed that leaf extracts of Albizia spp. showed better antibacterial activity compared to bark samples which were compatible with the results from GC-MS analysis.
Short Communication: Floral diversity of honey bee-collected pollen (Apis cerana) colonies in the Ir. H. Djuanda Forest Park, West Java, Indonesia Syayidah Nuriyah; Teguh Husodo; Wawan Hermawan; ARIEF ANSHORY YUSUF; Hikmat Kasmara; Joko Kusmoro; Indri Wulandari; Sya Sya Shanida
Nusantara Bioscience Vol. 13 No. 2 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130208

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Abstract. Nuriyah S, Husodo T, Hermawan W, Yusuf AA, Kasmara H, Kusmoro J, Wulandari I, Shanida SS. 2021. Short Communication: Floral diversity of honey bee-collected pollen (Apis cerana) colonies in the Ir. H. Djuanda Forest Park, West Jawa, Indonesia. Nusantara Bioscience 13: 185-193. Honey bees, especially Apis cerana Fabr., need feed sources from nectar and pollen. In addition, pollen is essential for sexual reproduction. However, various plants in the forest park have not been supported by information on pollinated plants or as feed sources by A. cerana. Therefore, this study should be conducted as essential information to reveal the pollens as feed sources of A. cerana in the Ir. H. Djuanda Forest Park, West Java. However, there is little information about those plants in the forest park, West Java. Therefore, this study aimed to reveal the pollens as feed sources of A. cerana in the Ir. H. Djuanda Forest Park. Sampling was collected on 45 honey bee-collected pollen from three cultivation areas. Pollen preparation was carried out using the acetolysis method. Based on the study results, 70 species of pollen flora of 36 families were found in Ir. H. Djuanda Forest Park vegetation in the A. cerana. However, further study is needed regarding the frequently visited plants used as feed sources or pollinating by A. cerana. In addition, considering that the honey bees cultivated in the forest park are dominated by A. cerana, further studies are needed on regularly pollinated plants to maximize the yield of honey cultivation.
Antioxidant and antibacterial screening of honey of Hiterotrogona itama collected from differents meliponiculture areas in East Kalimantan, Indonesia SUROTO HADI SAPUTRA; BERNATAL SARAGIH; IRAWAN WIJAYA KUSUMA; ENOS TANGKE ARUNG
Nusantara Bioscience Vol. 13 No. 2 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130213

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Abstract. Saputra SH, Saragih B, Kusuma IW, Arung ET. 2021. Antioxidant and antibacterial screening of honey of Hiterotrogona itama collected from different meliponiculture areas in East Kalimantan, Indonesia. Nusantara Bioscience 13: 232-237. Heterotrigona itama (Cockerell, 1918) is a stingless bee in several areas in East Kalimantan of Indonesia. This study aimed to analyze the phytochemicals and antioxidant activity of honey of this stingless bee from several regions in East Kalimantan. Phytochemical testing was carried out qualitatively, while antioxidant activity was performed using DPPH (1.1-diphenyl-2-picrylhydrazyl-radical) radical scavenging assay. Furthermore, the antimicrobial activity of Staphylococcus aureus and Escherichia coli was performed through in vitro tests. The results showed that the honey of stingless H. itama bee collected from five cultivated areas in East Kalimantan contained flavonoids, coumarin, steroids, and carotenoids, some of which had alkaloids and tannin compounds. The antioxidant activity (IC50) was observed between 43,54 ppm-71,27 ppm, while the strong antimicrobial activity of this honey was found against S. aureus and E. coli.
Trichoderma inoculants and straw compost improved resilience and yield in Cu-contaminated rice paddies Virginia Cuevas; Charina Gracia Banaay
Nusantara Bioscience Vol. 14 No. 1 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140101

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Abstract. Cuevas VC, Banaay CGB. 2022. Trichoderma inoculants and straw compost improved resilience and yield in Cu-contaminated rice paddies. Nusantara Bioscience 14: 1-9. Rice paddies in Marinduque, Philippines, are copper-contaminated from tailings of two mining companies formerly operating in the province. At present, paddy-soil copper concentration ranges from 22-386 mg kg-1. Crops suffer from copper toxicity and water stress due to climate-related events. The field study investigated the ability of in situ composted rice straw and Trichoderma microbial inoculant (TMI) to mitigate rice productivity constraints. In treated set-ups, rice straw was scattered on the paddy after harvest. Triple 14 mineral fertilizer was mixed with Trichoderma compost activator, broadcasted over the straws, and subsequently incorporated into the soil during land preparation. Rice seeds were TMI-coated before sowing. Rice straw composting was not done in control set-ups, and seeds were uncoated. Mineral fertilizers were applied to both set-ups. Furthermore, set-ups were categorized based on soil Cu content, such as normal, moderate, and high. Four replicates were made per season and category. Rice leaves did not show yellowing in treated paddies, indicating adequate N mineralization and plant uptake. The difference in yield was significantly higher (81%) in treated paddies compared to the control. During severe drought conditions, the mean yield in treated paddies was 1.8 t ha-1, while that of control paddies was zero. The yield was significantly correlated with K inputs, mainly by compost in treated paddies. Applying rice straw compost and Trichoderma inoculants can be an adaptive strategy for climate change resilience and mitigation of copper toxicity in crops.
Review: Natural products isolated from Portulaca oleracea (purslane, Ma-Chi-Xian): Focus on oleraciamides and oleracones CHRISTIAN BAILLY
Nusantara Bioscience Vol. 13 No. 2 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130210

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Abstract. Bailly C. 2021. Review: Natural products isolated from Portulaca oleracea (purslane, Ma-Chi-Xian): Focus on oleraciamides and oleracones. Nusantara Bioscience 13: 202-210. Portulaca oleracea L., purslane in English and Ma-Chi-Xian in Chinese, is largely used in traditional medicine to treat various diseases and conditions, including dysentery and urinary tract dysfunctions, and post-partum bleeding. It is also an edible plant with high nutritional potential. Extracts of P. oleracea display antioxidant, anti-inflammatory, and antiproliferative activities, associated with numerous bioactive secondary metabolites, including alkaloids, terpenoids, lignans, and polysaccharides. The present review focuses on two sub-groups of natural products isolated in recent years from P. oleracea: the alkaloids oleraciamides A-to-G and the oleracones A-to-L, which are mostly flavonoids. Their structural diversity and pharmacological properties, described in recent publications and patents, have been analyzed. These two subgroups of natural products deserve additional studies to delineate their mechanism of action. In addition, they could serve as a starting point for the design of novel anti-inflammatory agents, at least for some of them. This review provides a global view of these compounds, which is necessary to promote further phytochemical studies and better apprehend the traditional use of the plant and its extracts.
Antibacterial activity of Jatropha tanjorensis leaf extracts against bacteria associated with wound infections from the clinical setting OLAWALE OLADEJO BABAYEMI; EBENEZER AYODELE OKE; MICHAEL TOSIN BAYODE
Nusantara Bioscience Vol. 13 No. 2 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130215

Abstract

Abstract. Babayemi OO, Oke EA, Bayode MT. 2021. Antibacterial activity of Jatropha tanjorensis leaf extracts against bacteria associated with wound infections from the clinical setting. Nusantara Bioscience 13: 239-246. The use of microbial agents to treat infectious wounds based on ethnobotanical knowledge is still minimal. Antibacterial activity of Jatropha tanjorensis J.L.Ellis & Saroja leaf crude extracts and commercial antibiotics were evaluated against bacterial isolates associated with wound samples using agar well diffusion and disc diffusion techniques, respectively. Phytochemical analysis of the sections was carried out using standard methods. Saponin (58 mg/g) was the highest phytochemical in the methanol extract, while flavonoid (0.1 mg/g) was the lowest percentage in the cold water extract. Methanol extract had the highest Zone of Inhibition (ZOI) of 33 mm against Staphylococcus aureus coagulase-positive. In contrast, the lowest ZOI (5 mm) against S. aureus coagulase-positive was obtained from cold water extract. Methanol extract resulted in the highest ZOI ranging from 16 mm to 17 mm against Escherichia coli, while the lowest ZOI was obtained from coldwater extract (3-4 mm). The highest Minimum Inhibitory Concentration (MIC) value of all extracts (100 mg/mL) was obtained against P. aeruginosa, while the lowest MIC value was obtained against coagulase-positive S. aureus (12.5 mg/mL). All extracts contain Octadecanoic acid, n-hexadecanoic acid, and phytol. This study revealed that methanol extract had the highest inhibitory activity against bacteria isolated from wound samples compared to other crude extracts and ciprofloxacin. Therefore, Jatropha tanjorensis could be used as a potent herbal remedy to reduce the adverse effects of wound infection.
Plant-extract-mediated biosynthesis of silver nanoparticles using Eleutherine americana bulb extract and its characterization JULINDA ROMAULI MANULLANG; RUDY AGUNG NUGROHO; MIFTAKHUR ROHMAH; RUDIANTO RUDIANTO; AMANDA QORYSUCHI
Nusantara Bioscience Vol. 13 No. 2 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130216

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Abstract. Manullang JR, Nugroho RA, Rohmah M, Rudianto, Qorysuchi A. 2021. Plant-extract-mediated biosynthesis of silver nanoparticles using Eleutherine americana bulb extract and its characterization. Nusantara Bioscience 13: 247-254. The plant-based biosynthesis of nanoparticles has gained increasing momentum due to being lower in cost and eco-friendly. This study aimed to biosynthesize nanoparticles from the ethanolic bulb extract of Eleutherine americana (Aubl.) Merr. Ex K.Heyne (Ea-AgNPs), then characterize Ea-AgNPs and determine their phytochemical content and antioxidant capacity. The Ea-AgNPs were synthesized using ethanolic extract of E. americana bulb along with various concentrations of AgNO3 (0.5-4 mM). The Ea-AgNPs were then characterized using UV-VIS spectroscopic, Scanning Electron Microscopy/ Energy Dispersive X-ray (SEM/EDX), Transmission Electron Microscopy (TEM), X-ray Powder Diffractometry (XRD), and Fourier-Transform Infrared Spectroscopy (FTIR) techniques. The results indicated that E. americana could be used to reduce AgNO3 to synthesize Ea-AgNPs, indicated by color change, and had optimum UV/VIS spectra at 400 nm. The FTIR analysis found that Ea-AgNPs showed peaks at 2919, 2850, 1586, and 1031 cm1, containing several important bio compounds. Additionally, the XRD results found an amorphous Ea-AgNP peak with maximum intensity and proportion of silver occurring at 24 Theta. The particle size distribution curve of Ea-AgNPs showed a size of 105 nm. Furthermore, SEM/EDX analysis revealed an optical absorption characteristic peak at 3 keV. The EDX examination revealed three signals: a strong signal from the C atom (70.99 %), an O atom (28.95 %), and an Ag atom (0.06 %). The TEM imaging also showed the characteristics of Ea-AgNPs. Some phytochemicals such as flavonoids, tannins, alkaloids, and saponins were found in Ea-AgNPs, with IC50 values of 45.30 ppm.
Synthesis of novel polymer quaternary ammonium salt derived from glucose as a phase transfer catalyst IHSAN ALRUBAIE; AHMED THAMER SALIM; MOHAUMAN MOHAMMAD MAJEED; ALI JABBAR RADHI
Nusantara Bioscience Vol. 14 No. 1 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140103

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Abstract. Alrubaie I, Salim AT, Majeed MM, Radhi AJ. 2022. Synthesis of novel polymer quaternary ammonium salt derived from glucose as a phase transfer catalyst. Nusantara Bioscience 14: 25-33. Some new polymers containing quaternary ammonium salts based-carbohydrate starting from methyl-4,6-O-benzylidene-glucopyranoside have been prepared. The ammonium groups are connected to the glucose structure in various positions (2 and 3). Our synthesis used methyl-4,6-O-benzylidene-?-D-glucopyranoside through nucleophilic reagents to produce the main intermediates. The monomer glucose-quaternary ammonium salts are immobilized on the polyvinyl azide to give the final structure of the Polymer-Supported quaternary ammonium salts. In certain new kinds of phase transfer catalysts, the effectiveness of the polymer glucose-quaternary ammonium salts was tested in the Williamson etherification. Ether synthesis is a typical example of using a phase transfer catalyst. The reactions were performed in a liquid-liquid two-phase system, applying a mixture of toluene and a 50% solution of aqueous sodium hydroxide in the presence of a 20 mg/mmol catalyst (compound 9). Compared to other derivatives, the reaction of 4-nitrophenol with dibromoethylene gives the highest yields. On the other hand, compound 9 with the C16 chain gives high activity in the phase transfer catalyst.
Agronomic diversity of several soybean putative mutant lines resulting from gamma-rays irradiation in M6 generation NILAHAYATI NILAHAYATI; NAZIMAH NAZIMAH; RD. SELVY HANDAYANI; JAKA SYAHPUTRA; MUHAMMAD RIZKY
Nusantara Bioscience Vol. 14 No. 1 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140104

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Abstract. Nilahayati, Nazimah, Handayani RDS, Syahputra J, Rizky M. 2022. Agronomic diversity of several soybean putative mutant lines resulting from gamma-ray irradiation in M6 generation. Nusantara Bioscience 14: 34-39. Soybean is one of the foremost commodities for Indonesian people. Therefore, increasing domestic production must continue to be pursued absolutely. One way to do this is to use the assembly technology of new superior varieties with better and adaptive properties. This study aims to determine the agronomic diversity and yield of soybean putative mutant lines resulting from gamma irradiation in M6 ??generation. The research was conducted in Paloh Lada Village, Dewantara Subdistrict, Aceh Utara District, Indonesia, from July 2021 to October 2021. The tested genotypes consisted of 8: Kipas Putih variety (parent variety), Anjasmoro variety (comparison variety), M.1.1.3, M.5.2.1, M.5.2.3, M.1.1.8, M.1.1.9, and M.1.1.17 mutant lines. The results showed that the agronomic diversity of the tested mutants differed from the parents and comparison variety. In addition, we obtained early maturity and high yielding mutant in the M7 ??generation. The mutant line with a high yield and equivalent to its parents was M.1.1.8, with a production of 4.37 tons.ha-1. Mutant lines with early maturity were M.1.1.9, M.5.2.1, and M.1.1.3 lines with a harvesting age of 83 days after planting (DAP).