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Penanda Molekul DNA Mikrosatelit untuk Karakterisasi Bibit Jamur Kuping (Auricularia polytricha [Mont.] Sacc.) I Nyoman Pugeg Aryantha; Yuniar Mulyani; Rahmat Ariffudin
Jurnal Matematika & Sains Vol 13, No 1 (2008)
Publisher : Institut Teknologi Bandung

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This study aims to find out a method of spawn characterization for wood ear mushroom (A. polytricha [Mont.] Sacc.)based on microsatellite DNA marker. Four strains of wood ear mushroom cultivated in several regions of Java i.e AUC(West Java), AUCN (Central Java), AUP (West Java) and AUT (West Java) were evaluated. A pair of designed primers(5â -GGGAAAGTGATCCCATCTT-3â and 3â-AGTTGTGGGAACATCGAACT-5â) was able to amplify microsatelliteDNA from those four strains of wood ear mushroom. Strains AUC, AUCN and AUP share the same positions (2 loci) ofmicrosatellite motive (TC)n with PCR products of 212 and 201 bp, while the same motive for strain AUT was found atdifferent loci with PCR products of 127, 108, and 96 bp. The same motive was also found at another locus of strainAUC and AUP with a PCR product of 228 bp. Another different locus with a PCR product of 85 bp also gave positiveamplification result for strain AUC. The data on macroscopic characters (mycelial growth rate and quality, fruitingpercentage and mushroom yield) revealed that AUC, AUCN and AUP are good strains, while AUT is bad strain.Overall, there is a good correlation between molecular (microsatellite DNA) patterns and macroscopic data on mycelialgrowth rate and quality, as well as flushing percentage and mushroom yield. Therefore, microsatellite DNA motive of(TC)n can be adopted for spawn characterization of wood ear mushroom.

Aktivitas Antijamur Fusarium oxysporum Schlecht dari Tanaman Asli Indonesia Karlina, Yenni; Sukrasno, Sukrasno; Aryantha, I Nyoman Pugeg
Acta Pharmaceutica Indonesia Vol 38, No 3 (2013)
Publisher : School of Pharmacy Institut Teknologi Bandung

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Telah dilakukan penelitian aktivitas antijamur ekstrak metanol dari tujuh belas simplisia, yaitu rimpang dringo (Acorus calamus L.), umbi lapis bawang putih (Allium sativum L.), rimpang lengkuas (Alpinia galanga (L.) Wild.), rimpang kunyit (Curcuma domestica Val.), daun kayu manis (Cinamomum burmanii), daun sereh wangi (Cymbopogon nardus), herba urang aring (Eclipta alba), daun sirih (Piper betle L.), daun ketepeng (Cassia alata L.), rimpang temu putih (Curcuma zedoaria (Christin) Rosc.), rimpang temulawak (Curcuma xanthorrhiza Roxb.), rimpang jahe (Zingiber officinale Rosc.), bunga dan daun cengkeh (Syzygium aromaticum(L.) Merrill and Perry), kulit buah manggis (Garcinia mangostana L.), herba babadotan (Ageratum conyzoides L.),dan daun sirih merah (Piper crocatum ) terhadap pertumbuhan jamur Fusarium oxysporum Schlecht. Uji aktivitas antijamur menggunakan metode difusi agar dengan menghitung prosentase penghambatan pertumbuhan radial miselium jamur pada hari ke tujuh. Konsentrasi ekstrak metanol yang digunakan untuk uji aktivitas antijamur, yaitu 2,5%, 5%, dan 10%. Ekstrak metanol daun sirih, bunga dan daun cengkeh memiliki aktivitas antijamur tinggi, yaitu 76-100%, Ekstrak metanol kunyit, dringo, temulawak, jahe memiliki aktivitas < 50%. Ekstrak metanol babadotan, bawang putih, lengkuas, ketepeng, temuputih, sereh wangi, sirih merah, manggis, kayu manis, dan urang aring tidak memiliki aktivitas antijamur Fusarium.Kata kunci : Antijamur, Fusarium oxysporium, tanaman obat.AbstractAntifungal activity of methanol extracts from 17 crude drugs, i.e. rhizome of Acorus calamus, bulbs of Allium sativum L, rhizome of Alpinia galanga L., rhizome of Curcuma domestica Val., leaves of Cinamomum burmanii, leaves of Cymbopogon nardus, leaves of Eclipta alba, leaves of Piper betle L., leaves of Cassia alata L., rhizome of Curcuma zedoaria Christin., rhizome of Curcuma xanthorrhiza Roxb, rhizome of Zingiber officinale Rosc., flower and leaves of Syzygium aromaticum, peel of Garcinia mangostana, herbs of Ageratum conyzoides, and leaves of Piper crocatum against fungi growth Fusarium oxysporum Schlecht. Antifungal activity was tested using agar diffusion method by calculating the percentage inhibition of fungal mycelium radial growth on the seventh day of incubation. Concentrations of the methanol extracts used were 2.5%, 5%, and 10% w/v. Methanol extract of Piper betle leaf, flower and leaf of clove, had a high antifungal activity 76-100%. Methanol extract of turmeric, dringo, ginger, had activity <50%. Methanol extract of babadotan, garlic, galangal, candle bush, white turmeric, citronella grass, red betel, mangosteen, cinnamon, and bhringraj did not have antifungal fusarium activity.Keyword : Antifungal activity, Fusarium oxysporium , medicinal plants.

AKTIVITAS LARVISIDAL EKSTRAK ETIL ASETAT DAN HEKSANA DARI FILTRAT BEAUVERIA BASSIANA TERHADAP AEDES AEGYPTI Aryantha, I Nyoman Pugeg; Dwiantara, Wahyu Setyaji
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3618

Beauveria bassiana produces several metabolites that are toxic to insects so that it can be used as a biological insect control agent as an alternative to synthetic pesticides. The aim of this study was to determine the larvicidal activity of ethyl acetate and hexane extract from B. bassiana filtrate culture against Aedes aegypti 2nd instar larvae. This research was it cooked by determining the optimum age of spore inoculum of B. bassiana on the Potato Dextrose Agar (PDA) based on the number of spores and its viability. Afterwards, we determine the incubation time of B. bassiana in the Potato Dextrose Broth (PDB) in order to obtain filtrate culture which have highest mortality effects against Ae. aegypti 2nd instar larvae. B. bassiana filtrate culture was extracted with hexane and ethyl acetate and tested aegypti for larvicidal activity with a concentration range of 50, 100, 200, 300 ppm. The LC50 value was carried out by probit analysis. The results showed that ninth day old culture in the PDA was the optimum age of spore inoculum with the spore number and viability were 2.54 x 107 spore/mL and 93.46% respectively. The filtrate of sixth day old culture in PDB medium gave 100% mortality against 2nd instar Ae. Aegypti larvae. LC50 values of ethyl acetate and hexane extract were 117.28 dan 287.09 ppm. These results showed that the ethyl acetate and hexane extract of B. bassiana filtrate culture have biopesticide potential against 2nd instar Ae. aegypti larvae.

Pre-treatment and Suitable Reagent Enabled a Reliable and Consistent for Molecular Detection of Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc4) Listya Utami Karmawan; Fenny Martha Dwivany; Rizkita Rachmi Esyanti; I Nyoman Pugeg Aryantha
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

Fusarium wilt which is caused by the soil-borne fungus, Fusarium oxysporum f.sp. cubense (Foc), is one of important diseases in banana plant. Foc tropical race 4 (Foc4) is the most pathogenic race which could infect various banana cultivars including Cavendish cultivar which was previously considered as resistant cultivar. Molecular detection of Foc using PCR analysis is indispensable to determine the race of Foc. We demonstrate a faster DNA isolation procedure described in previous method by substituting sodium acetate precipitation with ammonium acetate precipitation without affecting the result. Based on our experience, some fungal genomes were troublesome to be amplified. We suggested pre-treatment step prior to amplification procedure by incubating fungal DNA in 65°C for 10 minutes for any samples of fungal genome, including stubborn samples, before mixing into PCR mix reagent. PCR reagents should be tested for stubborn samples since some of the reagents were unable to amplify the desired DNA fragment. Pre-treatment and the choice of robust PCR reagent should be taken into consideration for a reliable and consistent Foc4 molecular detection result.

Endophytic Fungi from Four Indonesian Medicinal Plants and Their Inhibitory Effect on Plant Pathogenic Fusarium oxysporum Nampiah Sukarno; Rohani Cinta Badia Ginting; Utut Widyastuti; Latifah Kosim Darusman; Sihegiko Kanaya; Irmanida Batubara; I Nyoman Pugeg Aryantha; Mashuri Waite
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.152

The medicinal plants Centella asiatica, Curcuma xanthorrhiza, Guazuma ulmifolia, and Hydrocotyle verticillata are widely used in Indonesian traditional medicine, but little is known about their associated endophytic fungi. This research aimed to study the diversity of endophytic fungi derived from functional parts of these plants and to evaluate their potential as antifungal agents against the plant pathogenic fungus Fusarium oxysporum. A total of 17 isolates of endophytic fungi were obtained: nine from leaves of G. ulmifolia, three each from leaves of C. asiatica and H. verticillata, and two from rhizomes of C. xanthorrhiza. The genus Colletotrichum was found in all plants studied, but each plant was associated with different species. Colletotrichum aeschynomenes was associated with C. xanthorrhiza, C. siamense was associated with C. asiatica, and C. tropicale was associated with G. ulmifolia and H. verticillata. The species Curvularia affinis, Diaporthe tectonae, Lasiodiplodia mahajangana, Parengyodontium album, Talaromyces trachyspermus, and Speiropsis pedatospora were found only in G. ulmifolia; while Didymella coffeae-arabicae and Muyocopron laterale were found only in H. verticillata. The endophytic fungi showed inhibition activity against F. oxysporum with inhibition values of 6.0-78.9%, T. trachyspermus JBd10 and C. affinis JBd14 gave the highest inhibition activity.

Characterization of Plant Growth Promoting Rhizobacteria (PGPR) on Capcissum annum Indah Juwita Sari; Indria Wahyuni; Rida Oktorida Khastini; Ewi Awaliyati; Andriana Susilowati; Enggar Utari; I Nyoman Pugeg Aryantha
Jurnal Biodjati Vol 6, No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.13191

Plant Growth Promoting Bacteria Rhizobacteria (PGPR) is one of the potential bacteria to enhance of Capsicum annuum through inhabitation the growth of pathogenic fungi. This study aimed to characterize PGPR in chili plants (Capsicum annuum). PGPR was isolated from the soil habitat of the red chili plant in Cilegon, Indonesia. Screening was then carried out with the dual culture method on Petri dishes and tested through in vivo method on the red chili plant. The selected bacteria were characterized morphologically, biochemically, and physiologically. The results revealed that there were 14 single isolates of bacteria from the roots of the red chili plants. The five single bacterial isolates, namely Azostobacter, Azospirillum, Pseudomonas, Serratia, and Beijerinckia have good potential as PGPR based on multiple culture screening by producing clear zones and positively effect the growth of chili plants.

Lokus SSR Berasosiasi Karakter Tahan Penyakit Mati-Pohon Durian Berdasarkan Bulked Pseudo-Segregant Analysis (SSR Loci Associated to Resistance Traits to Durian Die-Back based on Bulked Pseudo-Segregant Analysis) Panca Jarot Santoso; I Nyoman Pugeg Aryantha; Sony Suhandono; Adi Pancoro
Jurnal Hortikultura Vol 30, No 1 (2020): Juni 2020
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v30n1.2020.p9-20

Penyakit mati-pohon disebabkan cendawan Pythiaceae khususnya Phytophtora palmivora, Pythium vexans, dan Pythium cucurbitacearum menjadi salah satu kendala utama dalam budidaya durian. Di antara upaya pengendaliannya adalah melalui pemuliaan dan seleksi tanaman tahan berbasis molekuler menggunakan marka SSR. Penelitian untuk mengidentifikasi lokus SSR yang berasosiasi dengan karakter tahan penyakit mati-pohon pada durian telah dilaksanakan di Laboratorium Genetika Tumbuhan SITH-ITB dari bulan April sampai dengan Desember 2014. Penelitian dilaksanakan secara bulked pseudo-segregant analysis dua pool DNA durian tahan dan rentan. Amplifikasi lokus SSR menggunakan 77 pasang primer mikrosatelit berlabel fluorescent. Produk amplifikasi dibaca menggunakan GeneMarker v.2.4.0., setiap puncak pancaran fluorescent yang memiliki nilai intensitas tinggi dipilih sebagai alel. Pembandingan panjang alel dilakukan di antara dua pool dan pembanding aksesi tahan. Lokus yang memiliki alel berbeda antara dua pool tetapi memiliki alel sama dengan pembanding dianggap sebagai marka yang berasosiasi dengan sifat tahan durian terhadap Pythiaceae. Hasil analisis ditemukan tiga lokus mDz03F10, mDz4B2, dan mDz3B1 dengan motif berturut-turut (GAA)3.A(GA)4, (GAGT)2ttGAGT, dan (TTTTATG)2(GCCC)2 teridentifikasi sebagai marka yang berasosiasi dengan karakter tahan Pythiaceae. Hasil analisis ini memerlukan satu langkah validasi untuk meyakinkan keterpautan marka dengan karakter target sebelum digunakan sebagai marka molekuler.KeywordsDurian; SSR; BpSA; Tahan; PythiaceaeAbstractDie-back disease caused by Pythiaceae especially Phytophtora palmivora, Pythium vexans, and Pythium cucurbitacearum is one of the obstacles in durian cultivation. An effort to control this disease is through breeding and selection of resistant plants based on molecular assays such as SSR markers. Research to identify SSR loci associated with durian die-back resistance was done at Plant Genetics Laboratory, SITH-ITB from April to December 2014. The research was conducted through bulked pseudo-segregant analysis of two DNA pools, resistance, and susceptible durians. Amplification of SSR loci was carried out by using 77 fluorescent labeled primers. Amplification products were analyzed using GeneMarker v.2.4.0. Fluorescent peak with high intensity was considered as a selected allele. Comparison of allele length was executed amongst two pools and resistance reference. A locus showed different allele between two pools, while it given the same allele to reference was considered as SSR marker associated with Phytiaceae resistance. The analysis were found three loci, mDz03F10, mDz4B2, and mDz3B1 with motif of (GAA)3.A(GA)4, (GAGT)2ttGAGT, and (TTTTATG)2(GCCC)2 recpectively identified as SSR markers associated to die-back resistance. This result, therefore, requires further validation to convince markers association to target traits before they are used as molecular markers.

Assessment of double screening programmes via solid substrate fermentation (SSF) in a flask system and identification of lovastatin potential producer Syarifah A. Rashid; Darah Ibrahim; I Nyoman Pugeg Aryantha
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Local economical substrates namely rice bran and unprocessed brown rice was applied into fermentation condition to produce a potent secondary metabolite compound, lovastatin. A basis condition of fermentation viz. 70% (v/w) of moisture content (adjusted to pH 6.0), 1x107 spore/ml of inoculum size, mixture of 1:1 substrates and 7 days of incubation period, was applied into SSF system. During a preliminary test, all of 72 fungi disclosed positive dark spot onto the thin layer chromatography plate (TLC). In order to verify the existence of lovastatin, the secondary screening which involving high performance liquid chromatography (HPLC) was conducted. Out of 72, only 71 fungi were detected as lovastatin producers and the highest production was stated from SAR I isolate with 68.72±0.84 mg lovastatin/g dry substrate and 0.87±0.03 mg glucosamine/g dry substrate of fungal growth. SAR I isolate was identified via colony and microscopic morphologies. Through the observations, SAR I isolate was identical to Aspergillus niger

Screening and Identification of Mushrooms Growth Promoting Bacteria on Straw Mushrooms (Volvariella volvacea) Indah Juwita Sari; I Nyoman Pugeg Aryantha
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 1 (2021): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.60618

This research aimed to identify the indigenous Mushroom Growth Promoting Bacteria (MGPB) bacteria that can increase the growth of Volvariella volvacea. The research began by isolating indigenous MGPB from planting media of straw mushrooms in Karawang, Indonesia. The screening was performed to select bacterial isolates that can promote the highest growth of mushrooms by dual culture method on PDA media. There were 10 of the 58 highest bacterial isolates that have a positive effect on the vegetative growth of mushrooms. The 23K bacterial isolate was the most significant increase in mycelium growth compared to other isolates and bacteria-induced controls. A bacterial isolate 25K by gene analysis was identified by 16S rRNA (518F primer (5’- CCA-GCA-GCC-GCG-GTA-ATA-CG -3’) and 800R primer (5’- TAC-CAG-GGT-ATC-TAA-TCC -3’). The result from gene analysis shows that there are ~1550 base pairs products. BLAST analysis and phylogenetic tree adjustment results show that the closest diversity of this bacterial isolate 25K is Bacillus thuringiensis serovar konkukian str. 92-27 (equality value = 99%).

Aktivitas Antijamur Fusarium oxysporum Schlecht dari Tanaman Asli Indonesia Yenni Karlina; Sukrasno Sukrasno; I Nyoman Pugeg Aryantha
Acta Pharmaceutica Indonesia Vol. 38 No. 3 (2013)
Publisher : School of Pharmacy Institut Teknologi Bandung

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Telah dilakukan penelitian aktivitas antijamur ekstrak metanol dari tujuh belas simplisia, yaitu rimpang dringo (Acorus calamus L.), umbi lapis bawang putih (Allium sativum L.), rimpang lengkuas (Alpinia galanga (L.) Wild.), rimpang kunyit (Curcuma domestica Val.), daun kayu manis (Cinamomum burmanii), daun sereh wangi (Cymbopogon nardus), herba urang aring (Eclipta alba), daun sirih (Piper betle L.), daun ketepeng (Cassia alata L.), rimpang temu putih (Curcuma zedoaria (Christin) Rosc.), rimpang temulawak (Curcuma xanthorrhiza Roxb.), rimpang jahe (Zingiber officinale Rosc.), bunga dan daun cengkeh (Syzygium aromaticum(L.) Merrill and Perry), kulit buah manggis (Garcinia mangostana L.), herba babadotan (Ageratum conyzoides L.),dan daun sirih merah (Piper crocatum ) terhadap pertumbuhan jamur Fusarium oxysporum Schlecht. Uji aktivitas antijamur menggunakan metode difusi agar dengan menghitung prosentase penghambatan pertumbuhan radial miselium jamur pada hari ke tujuh. Konsentrasi ekstrak metanol yang digunakan untuk uji aktivitas antijamur, yaitu 2,5%, 5%, dan 10%. Ekstrak metanol daun sirih, bunga dan daun cengkeh memiliki aktivitas antijamur tinggi, yaitu 76-100%, Ekstrak metanol kunyit, dringo, temulawak, jahe memiliki aktivitas < 50%. Ekstrak metanol babadotan, bawang putih, lengkuas, ketepeng, temuputih, sereh wangi, sirih merah, manggis, kayu manis, dan urang aring tidak memiliki aktivitas antijamur Fusarium.Kata kunci : Antijamur, Fusarium oxysporium, tanaman obat.AbstractAntifungal activity of methanol extracts from 17 crude drugs, i.e. rhizome of Acorus calamus, bulbs of Allium sativum L, rhizome of Alpinia galanga L., rhizome of Curcuma domestica Val., leaves of Cinamomum burmanii, leaves of Cymbopogon nardus, leaves of Eclipta alba, leaves of Piper betle L., leaves of Cassia alata L., rhizome of Curcuma zedoaria Christin., rhizome of Curcuma xanthorrhiza Roxb, rhizome of Zingiber officinale Rosc., flower and leaves of Syzygium aromaticum, peel of Garcinia mangostana, herbs of Ageratum conyzoides, and leaves of Piper crocatum against fungi growth Fusarium oxysporum Schlecht. Antifungal activity was tested using agar diffusion method by calculating the percentage inhibition of fungal mycelium radial growth on the seventh day of incubation. Concentrations of the methanol extracts used were 2.5%, 5%, and 10% w/v. Methanol extract of Piper betle leaf, flower and leaf of clove, had a high antifungal activity 76-100%. Methanol extract of turmeric, dringo, ginger, had activity <50%. Methanol extract of babadotan, garlic, galangal, candle bush, white turmeric, citronella grass, red betel, mangosteen, cinnamon, and bhringraj did not have antifungal fusarium activity.Keyword : Antifungal activity, Fusarium oxysporium , medicinal plants.

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