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Iman Rusmana
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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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INDONESIA
Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 398 Documents
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Isolation and Identification of Endophytic Fungi from Srikaya Plants (Annona squamosa) Having Potential Secondary Metabolites as Anti-Breast Cancer Activity PRASETYAWAN YUNIANTO; SYOFI ROSMALAWATI; INDRA RACHMAWATI; WAHYUDI PRIYONO SUWARSO; WAHONO SUMARYONO
Microbiology Indonesia Vol. 6 No. 1 (2012): March 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (580.415 KB) | DOI: 10.5454/mi.6.1.4

Abstract

Annonaceous acetogenin was extracted from Annona squamosa (Srikaya) seeds. It has cytotoxic activity against cancer cells and lower toxicity compared to other cancer drugs. Endophyte from Annonaceae is expected to have similar extracted metabolites to the host, thus increasing the economic value. This research is a preliminary study to obtain active compounds with potential as anti-cancer agents from endophytic fungi of Srikaya plants. Four endophytic fungal strains were isolated from Srikaya plants (Annona squamosa) and identified based on 28S rDNA sequence. The isolates are SKY II.3.1, SKY I.1.2, SKY II.3.2, and SKY III.3.1, and have similarity with Fusarium sp Vega760, Fusarium sp NRRL 22354 NRRL223, Nectria rigidiuscula, and Fusarium sp BOL35, respectively. The identified isolates were fermented in liquid media for three weeks. The liquid and mycelium were extracted using ethyl acetate. Whole extract of each fermented isolate was partitioned and evaporated to obtain ethyl acetate extract. Cytotoxicity assay of ethyl acetate extract was carried out at level 100 ppm by Methyl Thyazole Tetrazolium (MTT) viability test towards MCF-7 (breast cancer cell). The result indicated that each ethyl acetate extract could inhibit the viability of cell MCF-7 with 11.34, 99.78, 91.48, and 96.84%, for SKY II.3.1, SKY I.1.2, SKY II.3.2, and SKY III.3.1, respectively. Based on the results of cytotoxicity assay on MCF-7 breast cancer cells, endophytic fungi isolates SKY I.1.2, SKY II.3.2, and SKY III.3.1 are potential as sources of anti-breast cancer compounds.
Molecular Analysis of Immune-Escape Mutants of Hepatitis B Virus from Local Clinical Samples CHANDRA JINATA; ERNAWATI ARIFIN GIRI-RACHMAN; DEBBIE SOEFIE RETNONINGRUM
Microbiology Indonesia Vol. 6 No. 1 (2012): March 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.113 KB) | DOI: 10.5454/mi.6.1.2

Abstract

Small hepatitis B surface antigen (sHBsAg) is used as a component of hepatitis B vaccine. Even though this vaccine is known to be effective in preventing hepatitis B disease, natural mutation may induce Hepatitis B Virus (HBV) to form immune-escape mutant. This mutant is not only capable of infecting hepatitis B-vaccinated people, but also causing commercial diagnostic assay failure. Immune-escape mutant is generally detected from amino acid change at Major Hydrophilic Region (MHR) of sHBsAg while the change occurred outside the region may also lead to immune-escape mutant formation. This research was aimed to investigate the presence of HBV immune-escape mutants in local clinical samples in Indonesia. sHBsAg gene of seventeen HBV samples from local patients were amplified by polymerase chain reactions then subjected to two-directional sequencing. The DNA sequences later were analyzed by bioinformatics programs. Fifteen out of seventeen samples were genotype B and subtype adw2, while the other two were genotype C and subtype adrq+. Among fifteen genotype B samples, twelve of them were not immune-escape mutants, two were immune-escape mutants that have been previously reported (Gln129Arg and Met133Leu), and one was a mutant outside MHR that has not been previously reported as an immune-escape mutant (Tyr161Ser). Both samples of genotype C group were not immune-escape mutants. As conclusion, by investigating seventeen local clinical HBV samples, it was known that two of seventeen samples were confirmed as immune-escape mutants and one of seventeen samples was a mutant outside MHR.
Molecular Identification of Yeasts Isolated from Dadih by RFLP-PCR and Assessment on Their Ability in Utilizing Lactate YOGA DWI JATMIKO; MIGUEL DE BARROS LOPES; MARY D BARTON
Microbiology Indonesia Vol. 6 No. 1 (2012): March 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.551 KB) | DOI: 10.5454/mi.6.1.5

Abstract

A wide variety of yeasts have been involved in traditional fermented foods, and potentially contributed to the development of product properties. The existence of indigenous yeasts in dadih, a traditionally fermented form of buffalo milk of West Sumatera, has been reported but an accurate identification is still required to be conducted. This study was aimed to identify yeasts isolated from dadih using a molecular approach, and to evaluate their lactate utilization. A total of 51 isolates were characterized and identified as Pichia jadinii and Candida stellimalicola using PCR amplification of the 5.8S - internal transcribed spacer region combined with restriction fragment length polymorphism (RFLP) analyses and gene sequencing. The former species were the dominant one in this tested product. Their ability to utilize lactate was demonstrated, indicating that they could modify the sensory characteristics of dadih, and hence interact with the indigenous lactic acid bacteria in dadih. The restriction profiles of the dadih yeasts can be used as a data base for rapid identification of yeasts in the future. Further work is still needed to elucidate the dadih yeast ecology.
Sub-Acute Toxicity of Pigment Derived from Penicillium resticulosum in Mice TATANG SOPANDI; WARDAH WARDAH
Microbiology Indonesia Vol. 6 No. 1 (2012): March 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1106.196 KB) | DOI: 10.5454/mi.6.1.6

Abstract

Pigments derived from Penicillium have different toxicities depending on the pigment components. This study was intended to evaluate the sub-acute toxicity of oral exposure of Balb/c mice to Penicillium resticulosum pigment. A total of 50 healthy adult male and female mice were divided into 5 treatment groups and different doses of pigment (0, 125, 250, 500, and 1000 mg kg-1 weight) were orally administered. Oral feeding of pigment with doses 125 to 1000 mg kg-1 body weight daily to adult mice did not cause mortality nor any clinical abnormalities. There were no significant differences in body, liver and kidney weights, nor liver and kidney functions of mice when pigment was given orally with intake doses of 125 to 1000 mg/kg body weight daily for 28 d in comparison to mice without pigment intake (control groups). There is a slight difference in liver histopathology of mice exposed to 500and 1000 mg/kg body weight of pigment for 28 d in comparison to mice control groups, although there were no differences in kidney histopathology. Thus, we can conclude that the pigment of P. resticulosum can be cathegorized as low toxic pigment and well tolerated at dose below 500 mg kg-1 body weight daily for 28 d.
Characterization of Moderately Thermophilic Bacteria Isolated from Saline Hot Spring in Japan YOKO KAWASAKI; MADOKA AOKI; YORIYASU MAKINO; HIROYUKI SAKAI; YUKI TSUBOI; JUNKO UEDA; KEIKO WATANABE; SHUICI YAMAMOTO; NORIO KUROSAWA
Microbiology Indonesia Vol. 5 No. 2 (2011): June 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (31.744 KB) | DOI: 10.5454/mi.5.2.2

Abstract

Twelve strains of moderately thermophilic bacteria were isolated from saline hot springs (55°C, pH 8.3) located in Odaito, Hokkaido, Japan. Based on their 16S rRNA gene sequences, all the strains were closely related phylogenetically each other, indicating that the strains belong to a single species. However, maximal growth temperature and enzymatic characteristics of individual strains were slightly different each other. Some of them grew well at 55°C but others did not. The β-galactosidase activity was also different among the strains. Therefore, the representative strains BEK6 and BEK11 were chosen from individual phenotype groups, and were used for further characterization. The nucleotide sequence of full-length 16S rRNA genes of the representative strains showed 96.6% similarity with Bacillus alveayuensis indicating that the strains belong to a novel species of the genus Bacillus. The cells were Gram positive, and showed both catalase and oxidase activities. The optimal growth temperature and pH of strain BEK6 and BEK11 were around 50°C and 7-8, respectively. They were able to grow in the medium containing 10% NaCl in contrast to B. alveayuensis which can grow in the medium with up to 4% NaCl. The strain BEK11 showed relatively strong protease and amylase activities implying the potential industrial uses of these enzymes under the saline condition.
Guide for Authors Iman Rusmana
Microbiology Indonesia Vol. 4 No. 1 (2010): April 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (47.293 KB) | DOI: 10.5454/mi.4.1.%p

Abstract

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Chitinolytic Bacteria Isolated from Chili Rhizosphere: Chitinase Characterization and Application as Biocontrol for Aphis gossypii
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.909 KB) | DOI: 10.5454/mi.4.3.%p

Abstract

Chitin, a common constituent of insect exoskeleton, could be hydrolyzed by chitinase. This research was conducted to select rhizobacteria isolated from the rhizosphere of chili pepper that produced chitinase and to examine their chitinase activity in degrading chitin of the Aphis gossypii. A total of 25 rhizobacteria isolates formed a clear zone when grown on chitin agar. Three of them had the highest chitinolytic index and were identified as Bacillus sp. strain I.5, I.21, and II.14. The II.14 was chosen for characterization of chitinase activity. The isolate showed maximum chitinase activity at 48-h-incubation. Maximum temperature and pH of the chitinase activity were 55°C and 7.0, respectively. The cell culture and the enzyme crude extract of the above three isolates were tested against A. gossypii and the result was compared to the control through microscopic observation. Hydrolytic analysis showed that the enzyme crude extract of these isolates were able to degrade chitin of insect exoskeleton since the first 3-h-incubation. Meanwhile, the cell culture treatment on the chitin showed degrading activity after 12 h (Bacillus sp. strain I.21 and II.14), and 9 h (Bacillus sp. strain I.5). Chitin degradation of A. gossypii exoskeleton by enzyme crude extract was better than the cell culture treatment. Chitinases produced by Bacillus sp. strains I.5, I.21, and II.14 are potential as biocontrol agents for A. gossypii.
Bioenergetic Analysis of FLAG Tagged-Subunit 8 of Saccharomyces cerevisiae Mitochondrial ATP Synthase I MADE ARTIKA
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (72.414 KB) | DOI: 10.5454/mi.4.3.%p

Abstract

The majority of cellular energy in the form of adenosine triphosphate (ATP) is synthesized by the F1F0-ATP synthase. The yeast mitochondrial F1F0-ATP synthase is a multisubunit complex that contains at least 17 different subunits grouped into F1 and F0 sectors. Subunit 8 of yeast mitochondrial ATP synthase is a hydrophobic protein of 48 amino acids encoded by the mitochondrial ATP8 gene. Subunit 8 has three distinct domains; an N-terminal domain, a central hydrophobic domain and a C-terminal domain. FLAG tag addition to the C-terminus of subunit 8 and its variants has facilitated elucidation of subunit 8's membrane topology. In order to analyze its detailed structure and function, a set of strains expressing FLAG tagged-subunit 8 and its variants were subjected to bioenergetic analysis at cellular and mitochondrial levels. Results obtained showed that the hydrophobic character of the central hydrophobic domain of subunit 8 is essential for functional coupling between F1 and F0 sectors, hence for mitochondrial ATP synthase function.
Genetic Diversity of Osmophilic Yeasts Isolated from Indonesian Foods with High Concentration of Sugar . RIDAWATI; BETTY SRI LAKSMI JENIE; ITA DJUWITA; WELLYZAR SJAMSURIDZAL
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (932.958 KB) | DOI: 10.5454/mi.4.3.%p

Abstract

Isolation of osmophilic yeasts from a total of 70 samples consisting of jam, sweet condensed milk, honey, sweet soy sauce, and palm sugar was conducted. Sixty-eight osmophilic yeasts were isolated from strawberry jam, pineapple jam, and honey from South Sumatera. No yeast was obtained from condensed milk, honey from Sumbawa, sweet soy sauce, and palm sugar. Sequence analysis based on the ITS region showed that isolates were identified as five species belong to two genera, Candida and Sterigmatomyces. Those isolates were distributed in 5 species, C. metapsilosis, C. etchellsii, C. parapsilosis, C. orthopsilosis, and S. halophilus. C. etchellsii was the predominant species in South Sumatera honey, while C. parapsilosis group was predominant species in jams. Those species were reported as osmophilic yeasts. In both jams and honey we found C. parapsilosis and C. metapsilosis, whilst C. orthopsilosis was found only in pineapple jam. Phylogenetic analysis based on sequence of ITS region showed that most of the osmophilic yeasts (67 out of 68 isolates) were located in the phylum Ascomycota and only one isolate Sterigmatomyces halophilus NN38 from pineapple jam was located in the phylum Basidiomycota.
A Comparison of Serological and Bacteriological Methods for Detection of Mycloplasma gallisepticum in Experimentally-Infected Chickens USAMAH AFIFF
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.834 KB) | DOI: 10.5454/mi.4.3.%p

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to Mycoplasma gallisepticum. Three antigens were used in this experiment. Antigen 1 was prepared from whole cell of M. gallisepticum, antigen 2 was a sodium dodecyl sulfate-solubilized preparation from whole cells, and antigen 3 was prepared by sonication of the whole cell antigen. The assay was then used to detect (anti)-M. gallisepticum antibodies in experimentally-infected chickens compared with serum-plate-agglutination (SPA), haemagglutination-inhibition (HI) tests, and tracheal culture. Data obtained in this experiment showed that there was a correlation between seropositivity and rate of isolation of M. gallisepticum. ELISA was found to be less sensitive, but more specific than SPA, and more sensitive than the HI test. The whole cell antigen gave the highest optical densities but was less specific than the other two antigens. The ELISA using all three antigens successfully identified the M. gallisepticum-infected chickens uniformly and positively through 14-35 days post infection, and correctly identified the control group as negative through the 35 day experimental period. The ELISA obviously has a place in the serodiagnosis of avian mycoplasma. Improved-specificity and -sensitivity of the M. gallisepticum antigen is desirable.

Page 30 of 40 | Total Record : 398

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