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Microbiology Indonesia

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Published by Perhimpunan Mikrobiologi Indonesia

ISSN : 19783477 EISSN : 20878575 DOI : -

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 398 Documents

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The Life Cycle of Synchytrium pogostemonis on Pogostemon cablin DONO WAHYUNO
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Synchytrium pogostemonis became a serious disease of patchouli cultivation in Indonesia, since it spread widely in many patchouli producing areas in Indonesia. The fungus caused warts on leaves, petioles and young stems of infected patchouli. The infected plant developed rosette habit, lost its vigour, was susceptible to drought period and finally died. Few information regarding the eco-biology and life cycle of the fungus were available The present research aimed at describing the life cycle of S. pogostemonis. The diseased patchouli was obtained through artificial inoculation. Mass inoculation was carried out by placing the healthy patchouli seedling close to diseased patchouli as source of inoculum and was watered regularly using the top head sprinkler . The infected leaves were observed both under disecting and light compound microscopes, and the existing fungal structure were recorded, described and measured. It was observed that S. pogostemonis is a long-cycle type fungus, the sexual reproduction was initiated by zoospores, followed subsequently by development of resting-structure spores, vesicles, sori, and sporangial formation.

Isolation and Characterization of Simian Retrovirus Type D from Macaca fascicularis and M. nemestrina in Indonesia DIAH ISKANDRIATI; UUS SAEPULOH; SILMI MARIYA; RICHARD F GRANT; DEDY DURYADI SOLIHIN; DONDIN SAJUTHI; JOKO PAMUNGKAS
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (AIDS) in Asian macaques. In the past, SRV isolates from macaques had only been identified at the US primate centers, outside the country of origin and after the animals had been introduced into a new environment. In this study, we report the first isolation, cultivation and molecular characterization of the type D simian retrovirus naturally infecting wild caught macaques in their natural habitats in the country of origin, in this case, Indonesia. When peripheral blood mononuclear cells (PBMC) from Macaca fascicularis (Mf) and M. nemestrina (Mn) were co-cultured with Raji human B-cell line, syncytia were observed microscopically and confirmed by immunofluoresence assay using antibody to SRV-2. Immunoblot analysis of purified Mf-ET1006 from cell culture supernatants demonstrated that the viral core and envelope proteins reacted with rabbit anti-SRV. Sequence analysis of Mf isolates in the viral envelope region revealed high homology to SRV-2 (94-96%). On the other hand, the homologies in the envelope region of Mn isolates were less than 80% to SRV-1, SRV-2, SRV-3 and Mf isolates. This study suggests that the isolate from Mn may be different from any other published SRV isolates.

Identification and Selection of Entomopathogenic Fungi as Biocontrol Agents for Aphis gossypii from South Sumatra SITI HERLINDA; CHANDRA IRSAN; REKA MAYASARI; SELLY SEPTARIANI
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Aphid, Aphis gossypii is a vector of curly virus disease. The damage of chili due to its feeding is only 35% and it can achieved 100% if the damage caused by the aphid as a vector. The objectives of this research were to explore, to isolate, to identify, and to select entomopathogenic fungi as biocontrol agents for A. gossypii. The fungi were explored using insect bait in soil and collected infected insects from South Sumatra, Indonesia. Then, the fungi were isolated and identified, and finally the bioefficacy tests were done using 1 x 106 conidia mL-1 against the third instar of A. gossypii. The explorations found 25 isolates of enthomopathogenic fungi consisting 10 isolates of Beauveria bassiana and 15 isolates of Metarhizium anisopliae. Selection of the fungi isolates on the aphid nymphs showed that isolate BPM isolated from Pseudoplusia chalcites caused the highest mortality rate (80.80%), while the lowest (47.20%) was caused by the isolate BAgTb isolated from A. gossypii. The shortest time needed to produce 50% mortality (Lethal Time 50) was 2.54 days (isolate of Chrysodeixis chalcites from Muarasiban). The longest time (3.66 days) was produced by isolate of Tenebrio molitor from Tanjung Raja.

Analyses of Precore and Core Promoter Mutations of Hepatitis B Virus in Patients with Chronic Hepatitis B in Surabaya, Indonesia . JUNIASTUTI; EDUARDUS BIMO AKSONO; TAKAKO UTSUMI; YOSHIHIKO YANO; . SOETJIPTO; YOSHITAKE HAYASHI; HAK HOTTA; FEDIK ABDUL RANTAM; HERNOMO ONTOSENO KUSUMOBROTO; MARIA INGE LUSIDA
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Mutations of precore (A1896) and core promoter (T1762/A1764) of hepatitis B virus can reduce HBeAg production. These mutations are frequently found in the late HBeAg seroconversion. However, it has been a controversy about the role played by precore and core promoter mutations in determining outcome of chronic hepatitis B. In the present study, the variability of precore and core promoter of hepatitis B virus were analyzed using PCR amplification and sequencing, according to the outcome (viral load and HBeAg/anti-HBe) in chronic hepatitis B patients in Surabaya. The study groups included 5 patients with uncomplicated chronic hepatitis B and 10 patients with chronic hepatitis B and liver cirrhosis in Dr. Soetomo Hospital, Surabaya. The control group included 6 blood donors obtained from Indonesia Red Cross, Surabaya. All groups were HBsAg positive. Precore mutation A1896 was predominant in all groups (60%-67% of each), together with precore variant T1858. As reported, precore variant T1858 is a prerequisite for precore A1896 and characteristic for viral genotype. Nevertheless, core promoter mutations T1762/A1764 were predominant only in LC patients (60%). All of these mutations were found mostly after HBeAg seroconversion (anti-HBe+). Of most samples with anti-HBe+, precore mutation was related with low viral load (<105 copies/mL), but core promoter mutations with high viral load (>105 copies/mL). Precore mutation A1896 was predominant in all groups, but core promoter mutations T1762/A1764 were only predominant in LC patients. The precore mutation alone is possible not critical to indicate a poor outcome, the core promoter mutations must be considered also.

Diversity of SCAR Markers of Pyricularia grisea Isolated from Digitaria ciliaris Following Cross Infection to Rice SRI LISTIYOWATI; UTUT WIDYASTUTI; GAYUH RAHAYU; ALEX HARTANA; MUHAMMAD JUSUF
Microbiology Indonesia Vol. 5 No. 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.5.1.1

Cross infection of Pyricularia grisea from grass to rice and vice versa has been reported, but genetic changes are not known yet. This research aimed at estimating the possibility of the genotype alteration in P. grisea dc4 isolated from Digitaria ciliaris, following cross infection to either rice cv. Kencana bali, Cisokan, and IR64 or Panicum repens, Cynodon dactylon, Digitaria sp., and Ottochloa nodosa. The genotypes were analyzed by employing three SCAR markers, Cut1; PWL2; and Erg2. The results indicated that the dc4 was only able to infect Kencana bali, Cisokan, and P. repens. The dc4 had only two out of three SCAR markers, Cut1 and Erg2. Host shift was followed by genotype alteration in two loci of SCAR. Isolates derived from lesions on Kencana bali (dc4-kb) and Cisokan (dc4-c) of the dc4 infection, both lost their Cut1 and gained PWL2. On the contrary, there was no genotype alteration from dc4 to isolate derived from P. repens of dc4 infection (dc4-pr). Neither the isolate dc4-kb that was cross-inoculated to Cisokan nor the dc4-c that was cross-inoculated to Kencana bali showed SCAR marker change. In comparison, race 173 isolate and those derived from Kencana bali and Cisokan did not show genotype alteration. All had two out of three SCAR markers, PWL2 and Erg2. The isolate 173 was adapted to rice. This indicated that genotype diversity of the dc4 might arise following host shift from grass to rice.Pyricularia grisea merupakan cendawan blas yang telah diketahui memiliki kisaran inang luas selain pada padi. Infeksi silang cendawan blas pada rumput ke padi dan sebaliknya telah dilaporkan, tetapi perubahan genetiknya belum dilaporkan. Tujuan penelitian ini menganalisis kemampuan infeksi silang dan perubahan genotipe P. grisea dc4 asal Digitaria ciliaris dalam perpindahannya ke padi cv. Kencana bali, Cisokan, dan IR64 atau rumput Panicum repens, Cynodon dactylon, Digitaria sp. dan Ottochloa nodosa. Genotipe P. grisea dianalisis melalui tiga marka SCAR, yaitu Cut1; PWL2; dan Erg2. Isolat dc4 memiliki 2 marka SCAR, yaitu Cut1 dan Erg2; tidak memiliki PWL2. Isolat dc4 hanya mampu menginfeksi silang Kencana bali, Cisokan, dan P. repens. Turunan isolat dc4 sebagai hasil infeksi silang ke Kencana bali (dc4-kb) dan Cisokan (dc4-c) menunjukkan perubahan genotipenya, yaitu Cut1 tidak teramplifikasi pada keduanya; PWL2 teramplifikasi;, serta Erg2 tetap teramplifikasi. Sebaliknya, turunan isolat dc4 sebagai hasil infeksi silang ke P. repens (dc4-pr) tidak mengalami perubahan genotipe. Turunan isolat dc4-kb sebagai hasil infeksi silang ke Cisokan, maupun turunan isolat dc4-c dari Kencana bali, juga tidak menunjukkan perubahan genotipe, yaitu tetap menunjukkan keberadaan PWL2 dan Erg2. Sebagai pembanding digunakan isolat ras 173 yang diisolasi dari padi. Genotipe isolat tersebut maupun turunannya, sebagai hasil infeksi silang ke Kencana bali dan Cisokan, tidak menunjukkan perubahan. Perubahan genotipe dc4 terjadi mengikuti pergantian inang dari rumput ke padi.

Glucose Biosensor Using Selected Indonesian Bacteria DYAH ISWANTINI; NOVIK NOVIK; . TRIVADILA
Microbiology Indonesia Vol. 5 No. 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

Microbial glucose sensors have been developed using Escherichia coli bacterial strains from Japan. However, there is interest in developing local bacteria as glucose sensors in Indonesia. In this research, the stability and the potential of a selected number of Indonesian bacteria as glucose biosensors was explored. Results of this study indicate that three of them, E. coli, Bacillus subtilis, and Thermus filiformis exhibit properties of high viability and stability at high temperature (30-60 ºC). Spectrophotometrical and electrochemical measurements showed significant absorbance values and highly stable current features for E. coli as indicated by its high capacity to produce glucose dehydrogenase. E. coli, B. subtilis, and T. filiformis produced currents of 3.25 µA, 0.2 µA, and 0.02 µA respectively, and E. coli also produced a much higher activity of glucose dehydrogenase. Electrochemical measurement using E. coli-modified carbon paste electrode allowed the determination of glucose concentration of up to 20 mM. Therefore, Indonesian E. coli has a high stability and can be used as a glucose biosensor

Screening of Actinomycetes Producing an ATPase Inhibitor of Japanese Encephalitis Virus RNA Helicase from Soil and Leaf Litter Samples SHANTI RATNAKOMALA; RONI RIDWAN; PUSPITA LISDIYANTI; . ABINAWANTO; UTAMA ANDI
Microbiology Indonesia Vol. 5 No. 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

Actinomycetes are commercially important microorganisms for the production of antibiotics, enzymes, inhibitors of enzymes, and other bioactive secondary metabolites. Some 853 isolates of actinomycetes were isolated from soil and leaf litter samples in Kupang NTT and Enrekang, South Sulawesi. Those isolates were then tested for inhibition of ATPase activity of RNA helicase from Japanese encephalitis virus (JEV), in order to identify a drug candidate for the treatment of JEV infection. Results revealed that 14 isolates have relatively high inhibition-activity on JEV ATPase activity of the JEV-RNA-helicase, which range from approximately 40.0-50.0% inhibition. The highest inhibition-activity was identified in Actinoplanes philippinensis 5-849 with 49. 9% of inhibition-activity and Streptomyces chartreusis 5-095 with 49.2% of inhibition-activity

Genome-Shuffling-Improved Acid Tolerance and Lactic Acid Production in Lactobacillus plantarum for Commercialization LITA TRIRATNA; BUDI SAKSONO; LINDA SUKMARINI; ASEP SUPARMAN
Microbiology Indonesia Vol. 5 No. 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

We applied genome shuffling to improve the acid tolerance of Lactobacillus plantarum, while simultaneously enhancing lactic acid production. The starting populations were mutant libraries generated by gradual low pH adaptation and ultraviolet irradiation and then which were subjected to recursive protoplast fusion. A library of shuffled mutants (fusants) with genetic exchange is achieved by repetition of this process. After three rounds of genome shuffling, we obtained the best performing fusant that grow better at low pH 4.0 and consume glucose faster than does the wild type. In addition, lactic acid production of this fusant was 64% higher than that of the wild type. These results demonstrated that the genome shuffling has been successful in engineering L. plantarum with multiple beneficial improved phenotypes. In the future, this technology is a promising candidate to accelerate poorly characterized strains for commercialization

Optimization of Human Interferon α2b Soluble Protein Overproduction and Primary Recovery of Its Inclusion Bodies RATIH ASMANA NINGRUM; DEBBIE SOFIE RETNONINGRUM; YEYET CAHYATI; HENI RACHMAWATI
Microbiology Indonesia Vol. 5 No. 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

The hIFN2b open reading frame has been constructed and overexpressed in Escherichia coli BL21(DE3). The yields of protein purified using nickel column from inclusion bodies (IB) and total soluble proteins were 3.46 mg and 2.57 mg in 1 L culture, respectively. This research was aimed to obtain optimal condition for high level overproduction of soluble hIFNα2b as well as primary recovery of hIFN2b from IB. We used two different conditions for obtaining soluble protein, i.e. induction temperatures and inducer concentrations, and three different conditions for inclusion bodies, i.e. centrifugation speeds, washing and solubilizing buffers. Induction using 0.5 mM of isopropyl thiogalactopyranoside at 25 °C yielded 8.9 mg hIFN2b in 1 L culture. The best recovery of IB was achieved when 10 000 g was applied for centrifugation, 1% Triton X-100 in 50 mM Tris Cl pH 8.0 as washing buffer, and 8M guanidine HCl in 50 mM Tris Cl pH 8.0 containing 800 mM 2-mercaptoethanol as solubilizing buffer were used. At this optimal condition the yield of hIFN2b from IB was 28.85 mg in 1 L culture. The total recovery of hIFNα2b at optimal condition was 50% from IB and 14% from soluble protein. hIFN2b from IB was refolded by 9 d dialysis in refolding buffer (0.2 mM EDTA, 0.25 mM ditiothreitol, 50 mM Tris and 0.4 M urea pH 8.0).

Ultrastucture of Wolbachia are Found in Somatic and Reproductive Tissue of Drosophila simulans and D. melanogaster ENDANG SRIMURNI KUSMINTARSIH
Microbiology Indonesia Vol. 5 No. 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

Ultrastructural observations shows Wolbachia in the testis of Drosophila melanogaster, where Wolbachia and cytoplasm are excluded from the mature sperm, and also found either in somatics and reproductive organ of D. simulans. Details of the ultrastructure of Wolbachia pipientis from the reproductive and somatic organs of D. melanogaster harbour Wolbachia-induce popcorn-effect and D. simulans harbour Wolbachia-induce cytoplasmic incompatibility are described as follows: the ultrastructure of Wolbachia in both of them were similar, distribution of Wolbachia in D. simulans was not restricted to the reproductive organs, they were also found in somatic tissues (muscle). Wolbachia can be present without causing detrimental effects. They are pleomorphic (rod shaped, elongate, oval or slightly bent). The size varies between 0.04 µm and 0.47 µm in diameter and between 0.26 µm and 1.2 µm in length. In a few cases Wolbachia seem to have been undergoing fission, but are still joint together by a vacuolar membrane. The microorganisms appear to have two membranes, a host membrane and a bacterial membrane. However, in some cases the microorganism seems to be surrounded by only one membrane, the possibility why only one membrane was visible might be due to loss during the embedding process

Page 31 of 40 | Total Record : 398

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Iman Rusmana

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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location
Kota tangerang,

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INDONESIA

Abstract

Abstract

Abstract

Abstract

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Abstract

Abstract

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Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Iman Rusmana

Contact Email rusmana13@yahoo.com

Phone +62217560536

Journal Mail Official microbiology.indonesia@gmail.com

Editorial Address kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location Kota tangerang, Banten INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Iman Rusmana

Contact Email
rusmana13@yahoo.com

Phone
+62217560536

Journal Mail Official
microbiology.indonesia@gmail.com

Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location
Kota tangerang,

Banten

INDONESIA