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JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya
Published by Universitas Negeri Malang
ISSN : 25496565     EISSN : 25496573     DOI : 10.17966
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
JC-T (Journal Cis-Trans) : jurnal kimia dan terapannya is published by Universitas Negeri Malang, Faculty of Mathematics and Science, Chemistry Department.
Arjuna Subject : Kimia(semua kategori) - Kimia (Lain-Lain)
Articles 101 Documents
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Pengolahan Limbah Batik dari UKM Batik Blimbing Malang dengan Metode Elektrokoagulasi Menggunakan Elektroda Besi dan Alumunium Jauhar Dziban Assauqi; Hanumi Oktiyani Rusdi; Khalishah Najla Nursyaf; Mohammad Ihya’ Ulumuddin; Nadia Paradipta Asha; Wahyu Aziz Siregar
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 8 No. 2 (2024)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um0260v8i22024p016

Abstract

Industri tekstil di Indonesia mengalami perkembangan pesat, terutama dalam produksi batik sebagai bagian dari industri tekstil. Penetapan batik sebagai warisan budaya tak benda oleh UNESCO memberikan dampak positif pada industri ini. Namun, proses produksi batik menghasilkan limbah cair yang mengandung pewarna sintetis dan bahan kimia berbahaya. Limbah ini, jika tidak diolah dengan benar, dapat mencemari lingkungan dan membahayakan organisme perairan. Metode pengolahan limbah cair batik seperti elektrokoagulasi telah terbukti efektif dalam menurunkan kadar pencemar seperti zat pewarna, BOD, dan COD. Dalam penelitian ini, penggunaan elektrokoagulasi dengan elektroda Fe dan Al berhasil menurunkan kadar COD hingga 96,7%. Selain itu, proses ini juga berpengaruh pada parameter lain seperti suhu, pH, CO2 terlarut, dan DO. Meskipun demikian, masih diperlukan penelitian lebih lanjut untuk memastikan efisiensi dan keamanan penggunaan metode ini dalam mengelola limbah cair batik secara luas.
Optimasi Fermentasi Keratinase oleh Bacillus sp. MD24 dengan Pendekatan Response Surface Methodology Lina Shobrina; Eli Hendrik Sanjaya; Mieke Alvionita; Suharti Suharti
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 9 No. 1 (2025)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um0260v9i12025p001

Abstract

Bulu ayam merupakan limbah yang dapat di daur ulang karena mengandung protein bernilai tinggi yang dapat dimanfaatkan untuk mikroorganisme. Namun, protein pada bulu ayam termasuk jenis protein keratin yang sulit dicerna oleh enzim protease umum karena adanya ikatan disulfida. Enzim protease spesifik yang dapat mendegradasi keratin pada bulu ayam adalah keratinase. Pada penelitian pendahuluan, telah berhasil mengisolasi bakteri penghasil keratinase Bacillus sp. MD24, diukur dengan pendekatan One Factor at A time (OFAT) melalui teknik solid state fermentation (SSF) menggunakan bulu ayam sebagai satu-satunya sumber karbon dan nitrogen, menghasilkan aktivitas keratinase sebasar 17 U/mL pada pH 8 dan 16 U/mL pada suhu 37oC yang diuji dengan substrat kasein. Namun, produksinya sangat dipengaruhi oleh faktor-faktor lingkungan mikroskopis dari bakteri, sehingga untuk memperoleh nilai aktivitas enzim keratinase yang maksimal diperlukan optimasi fermentasi keratinase. Pada penelitian ini, dilakukan dalam beberapa tahapan yaitu (1) Skrining Faktor-Faktor yang Mempengaruhi Fermentasi Keratinase melalui Pendekatan One Factor at A time (OFAT), (2) Penentuan Faktor-Faktor yang Berpengaruh Signifikan dengan pendekatan Plackett-Burman Design (PBD), dan (3) Penentuan Nilai Optimum Faktor-Faktor yang Berpengaruh Signifikan menggunakan pendekatan Central Composite Design-Response Surface Methodology (CCD-RSM). Tujuan penelitian ini untuk mengetahui nilai optimum dari faktor-faktor signifikan yang mempengaruhi fermentasi keratinase oleh Bacillus sp. MD24 menggunakan pendekatan tiga tahapan yaitu OFAT, PBD, dan CCD-RSM sehingga berdampak pada nilai aktivitas keratinase yang optimal. Hasil OFAT skrining tiga faktor yang mempengaruhi fermentasi keratinase oleh Bacillus sp. MD24 diperoleh nilai faktor dengan aktivitas keratinase tertinggi pada kondisi fermentasi yaitu suhu 37oC, pH 8, dan jumlah inokulum 1% (%v/v). PBD memberikan hasil dari ketiga faktor yang memiliki pengaruh signifikan terhadap fermentasi keratinase yaitu suhu dan pH, serta melalui pendekatan CCD-RSM diperoleh nilai optimum pada suhu 37,21oC dan pH 7,99, dengan nilai aktivitas keratinase prediksi sebesar 1848,35 ± 7,39 U/mL mendekati respon yang diamati secara aktual sebesar 1826,15 ± 8,92 U/mL.
Pemekatan Keratinase dari Bacillus sp. 24 untuk Meningkatkan Aktivitas Dehairing Suharti Suharti
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 2 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i22017p001

Abstract

Keratinase dapat diproduksi oleh Bacillus sp MD24 dengan menggunakan bulu ayam sebagai substrat. Ekstrak kasar keratinase tersebut terbukti dapat digunakan sebagai agen dehairing. Namun demikian, proses dehairing memerlukan waktu 72 jam karena rendahnya aktivitas keratinase. Penelitian ini bertujuan meningkatkan kemampuan keratinase sebagai agen dehairing. Dua hal telah dilakukan dalam upaya meningkatkan aktivitas ektrak kasar enzim yaitu peningkatan produksi enzim dan pemekatan enzim. Usaha untuk meningkatkan produksi enzim dilakukan dengan suplementasi media menggunakan sumber karbon tambahan berupa gula sederhana (sukrosa, glukosa, atau fruktosa). Sedangkan pemekatan enzim dilakukan melalui pengendapan enzim menggunakan amonium sulfat dengan tingkat kejenuhan 80%. Enzim hasil pengendapan diuji kemampuannya dalam dehairing kulit kambing pada berbagai waktu inkubasi. Penelitian menunjukkan bahwa suplementasi media dengan karbon sederhana memberikan hasil yang kontradiksi dimana penambahan sukrosa, glukosa, atau fruktosa justru menurunkan produksi ekstrak kasar keratinase. Sedangkan pemekatan mampu meningkatkan aktvitas enzim dari 8,79 U/mL sebesar 20,29 U/mL dan mampu menghilangkan bulu dengan waktu perendaman selama 48 jam.
Kajian Pengaruh Waktu dan pH Optimum dalam Adsorpsi Methyl Violet dan Methylene Blue Menggunakan Abu Daun Bambu Kuntari Kuntari
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 2 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i22017p014

Abstract

Adsorption of methyl violet and methylene blue has been done on using bamboo leaf ash. This researchaims to study the characterization adsorbent by FT-IR spectrophotometry and adsorption with the parametersinteraction time and pH. The concentration of the dye was determined quantitively by UV-Visspectrophotometer. FT-IR spectogram of bamboo leaf ash show that typical fingerprint in IR spectrogram ofaf asdeobent with Si-O and Al-O groups. The adsorption results showed that optimum methyl violet adsorptionat 12 minutes and pH 11 with adsorption capacity of 22.8738 mg/g and adsorption of optimum methylene blueat contact time of 8 minutes and pH 11 with adsorption capacity of 17.9989 mg/g
Aktivitas Antioksidan Ekstrak Biji Buah Areca vestiaria Giseke dan Fraksinya dengan Metode DPPH (2,2-diphenyl-1-picrylhydrazyl) Nur Candra Eka Setiawan; Hilda Amalia
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 2 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i22017p009

Abstract

Antioxidant widely used to prevent degeneratif desease and anti aging. The seed of Pinang yaki fruitscontain some compounds which have antioxidant activity. The purposes of this research were to know the antioxidant activity of ethanolic extracts and fractions of the seed of pinang yaki fruits and the IC50 value of its antioxidant activity. The samples of the seed were prepared separately by soxhlet extraction method with ethanol 96% as the solvent. Then fractionation process used three different solvents are n-hexane, ethyl acetate, and ethanol-water. Futhermore, the calculation of yield, phytochemical screening, and test of antioxidant activity using DPPH method. Yield calculation result showed ethanolic extract, fraction of nhexane, fraction of ethyl acetate, fraction of ethanol-water 17,1%, 10,8%, 18,2%, 52,8%. Phytochemical screening of Flavonoids ethanolic extract, fraction of ethyl acetate, fraction of ethanol-water showed positive contains Flavonoid. IC50 value of ethanolic extract, fraction of n-hexane, fraction of ethyl acetate, fraction of ethanol-water 94,5 µg/mL, 445,7 µg/mL, 44,7 µg/mL, 91,4 µg/mL. The conclusion of this research is extract of ethanol and the fraction of seed of pinang yaki fruits has the antioxidant activity. Fraction of ethyl acetate has the higher antioxidant activity 44,7 µg/mL.
Sintesis dan Karakterisasi Senyawa-senyawa Hidroksi Ester Minyak Jarak Cina (Castrol Oil) Parlan Parlan; Siti Marfu’ah; Yuyun Musabbikha
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 1 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i12017p001

Abstract

The utilization of castor oil as a lubricant is very limited, because it was only used on the machine hydraulic. This is because their highly viscosity. In addition, the double bond in ricinoleic acid (cys-12-hydroxy-9-oktadecenoic acid) as the main constituent of castor oil is easily oxidized. The conversion of double bonds in the castor oil into another group will increase its resistance to oxidation. This research aims to synthesize the compounds of the hydroxyl ester derivatives of castor oil by converting the double bond in the fatty acid into hydroxyl and ester groups. This study consists of two main steps: 1) epoxidation of castor oil with performic acids, 2) opening of the epoxide ring with formic acid to form hydroxyl ester compound. Analysis and identification of the compounds is done by thin layer chromategraphy and infrared spectrometry. Characterization of compounds synthesized using the refractive index and viscosity. The results showed that the hydroxyl ester compounds can be synthesized from castor oil by epoxide double bonds and followed by ring opening of epoxides with carboxylic acids. Based on the character of the refractive index and viscosity is known that hydroxyl ester derivatives of castor oil could potentially be used as a
Sintesis dan Karakterisasi Senyawa Kompleks dari Kadmium nitrat dan 1,3-bis(difenilfosfino)propana dengan Stoikiometri 1 : 1 Wawan Setiawan; Fariati; Effendy
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 2 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i22017p019

Abstract

Coordination compound of cadmium nitrate and dppp has not been reported. The purpose of this research are to synthesize coordination compoound of cadmium nitrate and dppp with 1:1 stoichiometry and determine its structure. The coordination compound obtained is colourless cry stalline solid having beam shape with melting point of 272-275̊C. Results of electrical conductivity and qualitativeC fkekfksnitrate ion tesindicates that the compound is a molecular one. EDX analysisshow the empirical formula C27H26CdN2O6P2. The accepted compound is [Cd(ONO2)2(dppp)] with distorted tetrahedral structure and average free bond energy -47.5 kJ/mol.
Pembuatan Bioetanol dengan Cara Hidrolisis Menggunakan Kertas Koran Bekas serta Pemurnian Menggunakan Agen Pengering (MgSO4, Na2SO4, dan CaCl2) Charisma Nurwiyono Putri; Budi Utami
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 1 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i12017p010

Abstract

This research was performed to: (1) utilize newspaper as raw material for bioethanol production and (2) examine the effectively of bioethanol purification of newspaper by using Na2SO4, CaCl2, and MgSO4 substances. This research was used by experimental method. First step of bioethanol production wasconducted using a pretreatment process to break down the lignin content. Hydrolysis of newspaper was done enzymatically using the cellulose enzyme. Bioethanol purification method of newspaper was done through destillation and desiccantion methods using MgSO4, Na2SO4, and CaCl2 as drying agent. Bioethanol characterization was determined by Gas Chromatography/Mass Spectroscopy (GCMS) and Fourier Transform Infrared Spectroscopy (FTIR). The results of this research indicated as follows: (1) newspaper can be used as raw material for second generation of bioethanol production with pretreatment process, enzymatical hydrolysis, fermentation, and destillation and (2) NaSO4, CaCl2, and MgSO4 substances can be used to purify bioethanol with MgSO4 as the most optimum substances for purifying bioethanol from newspaper.
Penentuan Ketidakpastian Pengukuran Kadar Kafein pada Biji Kakao (Theobroma Cacao L.) Menggunakan Spektrofotometri UV-Vis Devita Wijiyanti; Thorikul Huda
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 2 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i22017p022

Abstract

A study on the determination of uncertainty in measurement of caffeine content in cocoa beans by UV -Vis spectrophotometer method. The aim of this study was to determine the uncertainty of measurement of caffeine content in cocoa beans. Caffeine in cocoa bean extract was analyzed using UV-Vis spectrophotometer at 273 nm wavelength, obtained by linear regression equation y=0,0497x+0,0028 with determinant coefficient R2 0,9996. The results showed that caffeine content of 26.40 mg/g. LOD and LOQ values were 0.52 mg/L and 1.73 mg/L. The contributing factors of uncertainty are concentration (c), mass (m), dilution factor and precision with an estimated uncertainty of measurement of 3.11 mg/g. Test results can be reported with a value of 26.40 ± 3.11 mg/g.
Aktivitas Antibakteri Metabolit Sekunder dari Ekstrak Metanol Biji Alpukat (Persea americana Mill) Rini Retnosari; Sutrisno Sutrisno; Karim Handoyo
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 1 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i12017p016

Abstract

An antibacterial activity of secondary metabolite has been isolated from methanol extract of avocado seed (Parsea americana Mill). The isolation process has started with extracting using methanol, then fractioning and purifying by chromatography technique such as vacuum liquid chromatography and gravitation column chromatography. The white solid of K-1 component has been isolated from this process. The melting point of K-1 component was 67-70˚C and dissolved in chloroform, ethyl acetate, and ethanol. K-1 component has λmaks at 219,0 nm (π→π* transition) and 344,0 nm (n→π* transition). Based on IR spectrum interpretation, this component contained –OH group, -C=O group of ester, -CH bond, and C=C bond. K-1 component was predicted as saponin group. The antibacterial activity assay against Escherichia coli and Staphylococcus aureus indicated that methanol extract of avocado seed and K-1 component showed antibacterial activity toward both bacteria, so that this component has future chances for traditional medicine development.

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