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All Journal HAYATI Journal of Biosciences The Journal of Pure and Applied Chemistry Research JC-T (Journal Cis-Trans): Jurnal Kimia dan Terapannya Chemistry Education Practice Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Hydrogen: Jurnal Kependidikan Kimia Jurnal Ilmiah Kesehatan (JIKA) JPP (Jurnal Pendidikan dan Pembelajaran)

Suharti

Universitas Negeri Malang

Author-ID : 2421015

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Articles

OsDREB2A Gene Polymorphism Does Not Affect Salinity Tolerance Potency of Local Rice Varieties from Banten ‘Ainun Sayyidah Zakiyah; Elhah Nailul Khasna; I Kade Karisma Gita Ardana; Choirun Nita Fikriani; Nina Bunga Anggraini; Dwita Novitasari; Dwi Listyorini; Suharti Suharti
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.123

Indonesia possesses vast coastal agricultural land areas with high salinity. This high salinity is a threat to rice productivity which may decrease to 79.8%. Banten province of Indonesia houses many local rice varieties which have salinity tolerance potency based on its agronomical characters. The adaptability of rice plant in high salinity soil can be affected by OsDREB2A gene, which encodes transcription factors from several salt-tolerant signaling pathway genes. The aims of this study were to find out the possible polymorphism of OsDREB2A gene from Banten local rice varieties. The polymorphism of OsDREB2A analyzed by Single Nucleotide Polymorphism (SNP) and possible molecular structure. OsDREB2A gene was amplified using DNA from Tambleng, Bulu Putih, and Pare Caok varieties as templates through PCR method with a pair of specific primers. DNA fragments obtained were analyzed by means of series of analysis software. From those analysis we obtained 840 bp, 836 bp, and 850 bp gene fragments from Tambleng, Bulu Putih, and Pare Caok varieties, respectively. There is polymorphism of OsDREB2A due to some mutation yet does not massively alter its protein structure. From this study, we suggest that Tambleng, Bulu Putih, and Pare Caok varieties potential for salinity stress resistance genetically.

Analysis of Genetic Potential of Banyuwangi Local Rice (Oryza sativa L.) Based on Relative Expression of Homogentisate Geranylgeranyl Transferase (HGGT) and Granule-Bound Starch Synthase I (GBSSI) Gene Dyati Galuh Pratita; Tita Putri Milasari; Elhah Nailul Khasna; Sunarmi Sunarmi; Evi Susanti; Suharti Suharti; Delia Wahyu Pangesti; Annasa Sabatia; Dwi Listyorini
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.181

Rice nutrition including vitamin and amylose contents become important aspect for many people around the world. Rice with high amylose content (low glycemic index) is good for those with Diabetes mellitus. Tocotrienol, one precursor of Vitamin-E biosynthesis is catalyzed by enzymes encoded HGGT, while amylose biosynthesis is catalyzed by enzymes encoded GBSSI. The objective of this study was to find rice varieties with high tocotrienol and/or amylose content based on the expression of HGGT and GBSSI among eight Banyuwangi local rice varieties. Relative expression of HGGT and GBSSI was measured by qRT-PCR and analyzed using 2ΔCt method. Statistical analysis resulted in the significantly different of HGGT and GBSSI relative expression among samples. Relative expression of HGGT from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, Blambangan A3, Merah Bali, Blambangan A2, Berlian, Janur Kuning, and SOJ A3, respectively; while relative expression of GBSSI from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, SOJ A3, Janur Kuning, Berlian, Merah Bali, Blambangan A3, and Blambangan A2, respectively. Based on this research we conclude that Hitam Melik potentially produces higher tocotrienol and lower glycemic index than other studied varieties.

Characterization of Protease Soluble Collagen (PSC) From Milkfish Scales (Chanos chanos) Nia Lutfiana Lutfiana; Suharti Suharti; Evi Susanti
The Journal of Pure and Applied Chemistry Research Vol 8, No 3 (2019): Edition September-December 2019
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2019.008.03.506

The aim of this study was to characterize protease soluble collagen (PSC) obtained from milkfish scales, extraction using protease from proteolytic bacteria HTcUM7.1 isolate. The characterization included Fourier Transform Infra Red (FT-IR) spectra, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) profile, Field Emission Scanning Electron Microscopy (FESEM), denaturation temperature by Differential Scanning Calorimetric (DSC) and solubility. The resulting PSC from milkfish scales has white color, fiber with a length of about 20-60 µm, FTIR spectra and SDS-PAGE profile showed that PSC was collagen Type I and denaturation temperature was 145.48 °C, with maximum solubility at pH 1-3 and 1-2 % NaCl. Its high denaturation temperature value allows the collagen to be applied in the fields of medicines and cosmetics.

Surface Behavior of Rhodamin and Tartrazine on Silica-Cellulose Sol-Gel Surfaces by Thin Layer Elution Surjani Wonorahardjo; Anugrah Ricky Wijaya; Suharti Suharti
The Journal of Pure and Applied Chemistry Research Vol 5, No 2 (2016)
Publisher : Chemistry Department, The University of Brawijaya

Physical and chemical interactions are the principles for different types of separation systems as the equillibrium dynamics on surface plays a key-role. Surface modification is a way for selective separation at interfaces. Moreover, synthesis of gel silica by a sol-gel method is preferred due to the homogeneity and surface feature easily controlled. Cellulose can be added in situ to modified the silica features during the process. Further application for to study interaction of rhodamin and tartrazine in its surface and their solubilities in mobile phase explains the possibility for their separation. This paper devoted to evaluate the surface behavior in term of adsorption and desorption of tartrazine and rhodamin on silica-cellulose thin layer in different mobile phase. Some carrier liquids applied such as methanol, acetone, n-hexane and chloroform. The result proves tartrazine and rhodamin is separated and have different behavior in different mobile phase. The retardation factors (Rf) of the mixtures suggest complexity behavior on silica-cellulose surface.

Pemekatan Keratinase dari Bacillus sp. 24 untuk Meningkatkan Aktivitas Dehairing Suharti Suharti
JC-T (Journal Cis-Trans): Jurnal Kimia dan Terapannya Vol 1, No 2 (2017)
Publisher : State University of Malang or Universitas Negeri Malang (UM)

Keratinase dapat diproduksi oleh Bacillus sp MD24 dengan menggunakan bulu ayam sebagai substrat. Ekstrak kasar keratinase tersebut terbukti dapat digunakan sebagai agen dehairing. Namun demikian, proses dehairing memerlukan waktu 72 jam karena rendahnya aktivitas keratinase. Penelitian ini bertujuan meningkatkan kemampuan keratinase sebagai agen dehairing. Dua hal telah dilakukan dalam upaya meningkatkan aktivitas ektrak kasar enzim yaitu peningkatan produksi enzim dan pemekatan enzim. Usaha untuk meningkatkan produksi enzim dilakukan dengan suplementasi media menggunakan sumber karbon tambahan berupa gula sederhana (sukrosa, glukosa, atau fruktosa). Sedangkan pemekatan enzim dilakukan melalui pengendapan enzim menggunakan amonium sulfat dengan tingkat kejenuhan 80%. Enzim hasil pengendapan diuji kemampuannya dalam dehairing kulit kambing pada berbagai waktu inkubasi. Penelitian menunjukkan bahwa suplementasi media dengan karbon sederhana memberikan hasil yang kontradiksi dimana penambahan sukrosa, glukosa, atau fruktosa justru menurunkan produksi ekstrak kasar keratinase. Sedangkan pemekatan mampu meningkatkan aktvitas enzim dari 8,79 U/mL sebesar 20,29 U/mL dan mampu menghilangkan bulu dengan waktu perendaman selama 48 jam.

Efektivitas POGIL pada Pembelajaran Kesetimbangan Kimia terhadap Keterampilan Proses Sains dan Hasil Belajar Siswa dengan Kemampuan Awal Berbeda Ali Amirul Mu'minin; I Wayan Dasna; Suharti Suharti
Hydrogen: Jurnal Kependidikan Kimia Vol 8, No 1 (2020): Hydrogen: Jurnal Kependidikan Kimia
Publisher : Universitas Pendidikan Mandalika Mataram

Studying hydrocarbon material requires ability in problem solving so students are able to identify, analyze, and describe abstract chemical concepts and principles into concrete. The purpose of this study was to the improving of students' hydrocarbon problem solving abilities through the SAVI learning model assisted by puzzle media. The research design used in this study was pre-experimental with a research design namely One Group Pretest-Posttest Design.The study was conducted at SMAN 7 Mataram, Jl. Adi Sucipto, Ampenan Utara, Kec. Ampenan, Mataram City, West Nusa Tenggara. The subjects of this study were students of class XI Science. The research instrument include syllabus, lesson plans, worksheets, and multiple choice test items. Data were analyzed using the N-Gain test to find out the increased ability to solve hydrocarbon problems. The results showed that the level of N-Gain was in the range of 0.33 with a moderate category which meant an increase in students' problem solving abilities after using the SAVI model assisted by puzzle media. Thus, it can be concluded that students' problem solving abilities can be improved through the application of SAVI learning models assisted by puzzle media.

Pemberian Dietilnitrosamin dan Karbontetraklorida Pada Gambaran Histologi Ginjal Tikus (Rattus Norvegicus) Almas Dwi Khairana; Ricadonna Raissa; Wibi Riawan; Suharti Suharti; Hendra Susanto; Aulanni’am Aulanni’am
Jurnal Ilmiah Kesehatan (JIKA) Vol. 4 No. 1 (2022): Volume 4 Nomor 1 April 2022
Publisher : Sarana Ilmu Indonesia (salnesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36590/jika.v4i1.242

Diethylnitrosamine (DEN) is a metabolic product of various foods and beverages in which there are preservatives in the form of nitrate and nitrite. Nitrosamines are found in various products such as alcoholic beverages, processed meats, cigarettes, and cosmetic products. DEN is toxic and carcinogenic to organs such as the liver, lungs, kidneys, skin, stomach, and blood. In addition to giving DEN compounds, there are also CCl4 compounds as tumor promoter agents known as genotoxic substances that can increase the risk of genetic errors and stimulate cells to become malignant neoplasms. Administration of DEN and CCl4 induction can damage various organs of the body, one of which is the kidney. In a healthy body, angiogenesis plays a role in wound healing and the formation of new tissue. However, angiogenesis also contributes to carcinogenesis or the uncontrolled growth of cancer cells, and is malignant when the body is contaminated with toxic substances. Therefore, to determine the effect of the administration of DEN and CCl4 observations were made using the immunohistochemical method, to see differences in the histology of the kidneys of experimental animals, the positive control group, and the negative control group based on the VEGF angiogenesis factor. From these results, there are significant differences. In the negative group, there was no necrosis and high-value VEGF expression, but in the positive group, there was necrosis and a decrease in the value of VEGF expression compared to the negative control group.

Model Mental Mahasiswa Pada Fenomena Penghilangan Karat Melalui Elektrolisis Sunniarti Ariani; Effendy Effendy; Suharti Suharti
Chemistry Education Practice Vol. 3 No. 2 (2020): November
Publisher : FKIP University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/cep.v3i2.2104

Penelitian ini bertujuan untuk menggali model mental mahasiswa dalam memahami fenomena penghilangan karat melalui elektrolisis. Penelitian ini merupakan penelitian deskriptif dengan metode penelitian cross-section. Subyek penelitian adalah mahasiswa semester III dan V Program Studi Pendidikan Kimia FKIP Universitas Mataram yang dipilih dengan purposive sampling. Model mental mahasiswa digali menggunakan tes tertulis dan wawancara semistruktur. Model mental mahasiswa dianalisis menggunakan teknik constan comparative. Hasil penenlitian menunjukkan bahwa sebagian besar mahasiswa mengembangkan model mental inisial dalam memahami fenomena penghilangan karat melalui elektrolisis. Selain itu, ditemukan bahwa seiring dengan bertambahnya usia individu, model mental yang dikembangkannya mendekati model mental saintifik.Kata Kunci: model mental, karat, elektrolisis.

Gerakan Bersih-bersih Pantai Balekambang dari Sampah Plastik Nazriati Nazriati; Yudhi Utomo; Fauziatul Fajaroh; Suharti Suharti; Danar Danar; Endang Ciptawati
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol 5, No 2 (2020): July 2020
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v5i2.3573

The beach is an attractive place to unwind from your daily routine. The beauty of the beach must always be maintained so that comfort can still be obtained for everyone who visits it. The beach becomes a comfortable place if cleanliness is maintained. Plastic waste, if any along the coast, can be carried by the waves into the ocean. Plastic waste brought to sea can be consumed by marine biota because marine biota cannot distinguish between plastic and food waste. This community service activity intends to inspire visitors (tourists), managers and traders at Balekambang beach to care about the environment. A well-preserved environment will be a legacy that will be left on the next generation. The survey results show that visitors understand the meaning of waste and are willing to dispose of waste in its place. DOI: https://doi.org/10.26905/abdimas.v5i2.3573

Development and Validation of Higher Order Thinking Skills Based Instrument on Reaction Rate Subject Nevia Pradani; Suharti Suharti; Hayuni Retno Widarti
JPP (Jurnal Pendidikan dan Pembelajaran) Vol. 28 No. 2 (2021)
Publisher : Lembaga Pengembangan Pendidikan dan Pembelajaran, Universitas Negeri Malang in Collaboration with Asosiasi Pendidik dan Pengembang Pendidikan Indonesia (APPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar

This development aims to produce a product in the form of a HOTS-based instrument on the reaction rate subject. The development model used refers to the ADDIE development model carried out in three stages: analysis, design, and development. The developed question instruments were validated by the expert validator and then ran a small-scale test. Data analysis obtained value validation of instruments at 93.33% and included very good criteria. The 15 items developed were declared valid and reliable with the Cronbach's Alpha value equal to 0.805. As much as 10 items are classified as moderate, 1 item is classified as easy, and 4 items are classified as difficult and 3 items out of 15 items have an ineffective distracting effect. Based on these results, it can be concluded that the question instrument is suitable for use.

Co-Authors Ali Amirul Mu’minin Almas Dwi Khairana Annasa Sabatia Anugrah Ricky Wijaya Aulanni'am, Aulanni'am Choirun Nita Fikriani Ciptawati, Endang Danar Danar Delia Wahyu Pangesti Dina Yuli Agustin Dwi Listyorini Dwita Novitasari Dyati Galuh Pratita Effendy Effendy Elhah Nailul Khasna Endang Budiasih Evi Susanti Fauziatul Fajaroh Findiyani Ernawati Asih Gusti Wahyuni Hayuni Retno Widarti Hendra Susanto I Kade Karisma Gita Ardana I Wayan Dasna Nazriati Nazriati dkk. Nevia Pradani Nia Lutfiana Lutfiana Nina Bunga Anggraini Noni Asmarisa Punaji Setyosari Ricadonna Raissa Suhadi Ibnu Suhadi Ibnu Sunarmi Sunniarti Ariani Surjani Wonorahardjo Sutopo Sutopo Tita Putri Milasari Verdiana Somi Koten Wibi Riawan Widy Ika Parastuti Yudhi Utomo ‘Ainun Sayyidah Zakiyah

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