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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 8 Documents

Search results for , issue "Vol. 6 No. 2 (2016)" : 8 Documents clear

Microbial Community Composition of Two Environmentally Conserved Estuaries in the Midorikawa River and Shirakawa River Tran Thanh Liem; Mitsuaki Nakano; Hiroto Ohta; Takuro Niidome; Tatsuya Masuda; Kiyoshi Takikawa; Shigeru Morimura
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2016.006.02.02

To provide a general overview of the microbial communities in environmentally conserved estuaries, the top 5 cm of sediment was sampled from the sandy estuary of the Shirakawa River and from the muddy estuary of the Midorikawa River. Higher amounts of organic matter were detected in the Midorikawa estuary sample than in the Shirakawa estuary sample. Measurement of redox potential revealed that the Shirakawa estuary was aerobic and the Midorikawa estuary was much less aerobic. Clone analysis was performed by targeting partial 16S rRNA gene sequences and using extracted DNA from the samples as a template. Various bacteria were detected, among which Gammaproteobacteria was dominant at both estuaries. Unclassified clones were detected in the Gammaproteobacteria group, mainly among samples from the Midorikawa estuary. Other detected bacterial groups were Alphaproteobacteria, Deltaproteobacteria, Chloroflexi, Actinobacteria, and Bacteroidetes. All the Deltaproteobacteria clones were anaerobic sulfate-reducing bacteria. Those aerobic and anaerobic bacteria coexisted in the top 5 cm of the estuary sediments indicating the surface layer have active sulfur and carbon cycle. Abundance of aerobic Gammaproteobacteria may be an indicator for conserved estuaries. Keywords: conserved environment, clone analysis, estuary, microbial community, 16S rRNA gene.

Exploration and Antagonistic Test of Endophytic Fungi from Soybean (Glycine max L. Merr) With Different Resistance to Sclerotium rolfsii Siti Aminatuz Zuhria; Syamsuddin Djauhari; Anton Muhibuddin
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

The research aimed to determine the diversity of endophytic fungi in soybean crop with different resistance against Sclerotium rolfsii and find out their potential antagonist in controlling S.rolfsii by in vitro and in vivo. Materials used in this study were soybean with a variety of Wilis (susceptible variety) and Sinabung (resistant variety). This research was conducted at the Microbiology Laboratory of Central Laboratory of Life Science (LSIH), Brawijya University and in the trial plantation of Malang Research Institute for Food Crops, Lawang, Malang subdistrict in September 2015 until May 2016. Type of experimental design used was Complete Randomized Design with 16 treatments and three times repetitions at in vitro experiment with 16 treatments and four times repetitions at in vivo experiment. Observation on in vitro test is covering to colony morphology of fungal pathogens on PDA medium. On the test of in vivo, it was observed a disease occurrence and effectiveness rate of endophytic fungi. There are 15 species of endophytic fungi produced from isolation, namely Trichoderma sp., Aspergillus sp.2, Aspergillus sp.3, Acremonium sp.1, Acremonium sp.2, Acremonium sp.3, Acremonium sp.4, Fusarium sp.1, Fusarium sp.2, Cephalosporium sp, Microsporum sp., Penicillium sp., and unidentified fungi called W2 and W4. The highest inhibitory of endophytic fungi against S. rolfsii by Aspergillus sp.2 is 89.18% (in vitro) and 61.21% (in vivo), while Trichoderma sp. 91.88% (in vitro) and 63.29% (in vivo). Diversity index value of Wilis variety is higher than Sinabung, i.e. 1.878 and 1.606 respectively. While dominance index value of Sinabung variety is 0.2035 and Wilis is 0.1528. Keywords: Endophytic fungi, diversity, S. rolfsii.

Rosella Flower Decreases the CML Serum and Liver Inflammation of Rats Given Baked-Food Diet Silvy Amalia Falyani; Setyawati Soeharto; Edi Widjajanto; Ardhiyanti Puspita Ratna; Pia Bataif Batmomolin
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2016.006.02.03

Advanced Glycation Endproducts (AGEs) or so-called glycotoxin can be triggered by heated food in which the Maillard reaction occurs. One type of glycotoxins is CML. Accumulated N-Carboxymethyl-Lysine (CML) can cause inflammation of organs, e.g. liver. Rosella flowers contain anthocyanin compound that has anti-glycation and antioxidant effects. This study aimed to determine the effect of ethanol extract of Rosella on CML serum level, IL-6 level, and NF-ƙB activation in the liver of mice fed with baked food. This study used post-test design using 25 Wistar rats aged 3-4 months old that were divided into 5 groups, namely negative control group, positive control group, treatment groups given Rosella extract dose of 200mg.kgBW-1, 300mg.kgBW-1, and 400mg.kgBW-1. Baked feed was given for 12 weeks, and Rosella ethanol extract was administered in the 9th to 12th week. The examination on CML serum and IL-6 of the liver was using ELISA method. Immunofluorescent staining was used to determine NF-ƙB activation in the liver using a confocal microscope. CML serum is proven to increase significantly (p = 0.000). The effective dose of Rosella flower extract to prevent CML Serum increase is 200 mg.kgBW-1, whereas a dose of 400 mg.kgBW-1 can decrease IL-6 level and NF-ƙB activation. Ethanol extract of Rosella flower decrease the levels of N-carboxymethyl-lysine serum, IL-6, and NF-ƙB activation in the liver of rats given baked-food diet. Keywords: Advanced Glycation Endproducts (AGEs), Interleukin-6, N-Carboxymethyl-lysine, Rosella.

The Quality of Ornamental Japanese Carp (Cyprinus carpio var. Koi) After Electroporation as a Gene Material Transfer Method Saidah Luthfiyah; Agoes Soeprijanto; Yuni Kilawati
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Spermatozoa are male gametes that have an advantage in acting as gene transfer media (SMGT), because in transferring genetic material, sperm uses relatively natural vectors. This study was conducted to know sperm quality of Koi after short electroporation with different voltages using Gene Pulser. The study was conducted using a complete randomized design (RAL) with three (3) replications and five (5) treatments, namely: 10 V.cm-1, 20 V.cm-1, 30 V.cm-1, 40 V.cm-1, and 1 control. Electroporation was done with square wave, 1 ms duration of electroporation, 4 times of electroporation shocks with 0.1 s duration. The results showed that electroporation with different voltage levels significantly affected the motility and viability of sperm. Based on orthogonal polynomial analysis, it was known that the treatment affected motility and viability of sperm with a linear equation y = -0.9x + 5, 4 R² = 0.920 and r = 0.96 for motility and y = -11.26x + 91.61 R² = 0.823 and r = 0.91 for viability, meaning that the higher the level of electroporation the lower the viability and sperm motility produced. From the results of this study, it can be suggested that the electroporation of Koi sperm as a gene transfer medium should be performed at a voltage of 10 V.cm-1. Keywords: electroporation, Koi, sperm quality, transgenesis.

The Effect of Treatment Ant Nest (Myrmecodia pendans) on Quail Production and Performance (Coturnix-coturnix japonica) Susan Elfitri Lumban Gaol; Edhy Sudjarwo; Irfan Hadji Djunaidi
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

This research was aimed to determine the effect of ant nest treatment (Myrmecodia pendans) on quail production and performance. Research sample was 250 quails. Ant nest was administered in 5 treatments and 5 replications: P0 (0%) as control treatment, P1 (0.2%), P2 (0.4%), P3 (0.6%), and P4 (0.8 %) treatments. Data were collected from quails at 28 days to 70 days old. Variables measured were the performance and quail's egg production. This study was designed using completely randomized design. Real significant difference was examined utilizing Duncan's Multiple Range Test (DMRT). Analysis of variance result showed that ant nest provides significant influence to feed intake and Hen Day Production (P <0.05). The highest average of feed consumption is treatment P4 (0.8%), whereas treatment P0 (0%) was the lowest average for feed consumption. Feed conversion rate in treatment P4 (0.8%) was more efficient compared to other treatments. Ant nests administering did not result significantly different (P> 0.05) to feed conversion and egg mass. The results of the study concluded that higher ant nest administration is capable of increasing quails feed intake and Hen Day Production. Keywords: basal feed, flavonoids, productivity.

The Use of Full-Grained, Fragmented, and Reduced Marine Yeast Powder in Fodder Formulation to Improve the Growth of Eel Fish (Anguilla bicolor) Ria Retno Dewi Sartika Manik; Arning Wilujeng Ekawati; Hardoko Hardoko
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Ikan Sidat or eel fish (Anguilla bicolor) is a popular fish commodity in domestic trade as well as for export commodity. The use of marine yeast as fodder suffers from some weaknesses since it contains nucleic acids and rigid thick cell walls that obstruct the absorbance of nutrition. Optimizing the use of marine yeast as fodder ingredients can be done by fragmenting and reducing the yeast. This research attempts at optimizing the use of marine yeast regarding its rate of growth, fodder efficiency and the survival rate of eel fish. This research employs the complete random design using 4 different treatments and 3 repetition. In the four treatments, the fodder contained protein (45%) and iso energy (3.600 kkal.kg-1 fodder), which were applied to the treatment A 0% (control), B 5% (complete), C 5% (fragmented) and D 5% (reduced). The parameters used in this study were survival rate, specific growth rate, ratio of fodder conversion, ratio of protein efficiency, energy retention, protein retention and rate of metabolism. The data of this study showed the best result in the treatment D, 5% (reduced), showing survival rate value of 95.56%, specific growth at 1.12, food conversion ratio of 2.27, protein efficiency ratio of 0.98, protein retention at 18.26%, energy retention of 16.57, and protein metabolism rate at 81.96%. Keywords: eel fish, growth, marine yeast.

Modified Risk Factors for Coronary Heart Disease (CHD) in Minahasa Ethnic Group From Manado City Indonesia Ester Jeini Nelwan; Edi Widjajanto; Sri Andarini; M. Sasmito Djati
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Coronary heart disease (CHD) is a non-transmitted disease which is particular concern at the global, national, and local level. CHD has become a concern because it has caused a lot of deaths. Risk factors linked with CHD consist of permanent risk factors and variable risk factors. The purpose of the research is to analyze the correlation between variable risk factors and CHD in the Minahasa ethnic society in Manado City. This research was an observational case-control study conducted by Prof. dr. R. D. Kandou Hospital, Manado, from August to October 2016. In total, there were 220 patients included. The sampling used a simple random sampling method and the data obtained were analyzed by chi-squared test. According to the results, hypertension, smoking, and behavioral type were connected to cases of CHD. The correlation degree showed that the respondents suffering hypertension were 5.70 times more likely to suffer CHD, the smoking respondents were 2.25 times more likely to experience, and behavior type A respondents were 2.96 times more likely to suffer CHD. Hypertension, smoking, and behavioral type are linked with CHD, so there should be some promotion and preventive actions from the health governmental institution to society, especially aimed at adults, about the quality of life enhancement by healthy behavior and avoiding CHD risk factors. Keywords: behavioral type, Case-control study, hypertension, smoking behavior.

Lethal Concentration 50 (LC50 - 96 hours) Nile Tilapia (Oreochromis niloticus) exposed Cypermethrin-based Pesticide Shinta Hiflina Yuniari; Asus Maizar Suryanto Hertika; Amin Setyo Leksono
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

In aquaculture of an irrigation, the use of cypermethrin-based pesticide can harm nile tilapia which cultured within and its impact on humans who consume the fish. Thus it is necessary to study to determine the threshold of this cypermethrin-based pesticide. Determining the thresholds was using the test of LC50 - 96 hours (Lethal Concentration 50-96 hour) which tested tilapia that dead by 50%, which can be used as a benchmark threshold for cypermethrin-based pesticide. Test of LC50 - 96 hours consist of preliminary test and advanced test. Lethal Concentration 50 (LC50 - 96 hours) of cypermethrin-based pesticide towards nile tilapia (Oreochromis niloticus) is 0.082 ppm. At these doses, tilapia death reached 50%. This concentration is expressed as a threshold usage of cypermethrin-based pesticide on the environment around the aquaculture of tilapia. Keywords: Cypermehrin-based pesticide, LC5096 hour, Nile tilapia.

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA

Abstract

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA