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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 13 Documents

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Search results for , issue "Vol. 9 No. 2 (2019)" : 13 Documents clear

Alcohol Intake Investigation of Adult Rats Based on Sperm Parameters Choirul Anam; Agung Pramana Warih Marhendra; Sri Rahayu
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

The prevalence of alcohol consumption is high among adult males during the reproductive period. The current study aimed to evaluate the impact of chronic administration ethanol on the quality of sperm in the rats. Twelve healthy Wistar male albino rats were randomly divided into 2 groups to represent the 2 phase duration. The first phase lasted 21 days and the second phase lasted 49 days. In each phase, the animals were separated into subgroups: A and B. Subgroup A represented control that received distilled water while subgroup B represented animals that received 7 mL.kgBW-1 of 30% ethanol per day, thrice a week. The data were analyzed using ANOVA (P<0.05). There is no significant difference in sperm concentration and viability. However, there is a significant difference in the motility of spermatozoa between the control group and ethanol-treated group. Thus, the study indicates that ethanol administration may disturb the sperm motility and have no clear effect on its concentration and viability.Keywords: alcohol consumption, reproductive function, sperm quality.

Optimization of Time and Temperature Gelatin Extraction from Pink Perch (Nemipterus bathybius) Head using Response Surface Methodology (RSM) Ulfatul Mardiyah; Simon Bambang Widjanarko; Kiki Fibrianto
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Gelatin from fisheries byproducts was very potential to be used as an alternative gelatin substitute for bovine and porcine gelatin. In this study, optimization of pink perch head gelatin extraction was carried out based on temperature and extraction time. Factorial design and Response Surface Methodology (RSM) were used to optimize the gelatin extraction process of pink perch (Nemipterus bathybius) head. The purpose of this research was to determine the optimum condition of the pink perch head extraction process based on the temperature and time of extraction. The extraction temperature (50°C - 90°C), and the extraction time (3 - 7 hours) were the independent variables. The response variables of this study were the yield of gelatin (%), gel strength (g.bloom) and viscosity (cP). The optimum extraction conditions were obtained at the extraction temperature of 74.40°C for 5.42 hours with a yield of 5.31%, the gel strength of 311.01 g.bloom and the viscosity of 5 cP. Considering its similarity to the commercial gelatin, particularly on their chemical characteristics, it can be suggested that the head of pink perch is a potential alternative of gelatin source. Keywords: Extraction, Gelatin, Optimization, Pink Perch Head

Heavy Metal (Pb) and Its Bioaccumulation in Red Algae (Gracilaria sp.) At Kupang Village, Jabon Sub-District, Sidoarjo District Yatris Rambu Tega; Endang Yuli Herawati; Yuni Kilawati
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Seaweeds have an inherent capacity to absorb heavy metals from marine water. This intrinsic ability allows these organisms to accumulate much amount of heavy metals over time. This study conducted at Kupang Village, Jabon Sub-District, Sidoarjo District, where almost 40% of the area consists of Gracilaria sp. ponds cultured. The purpose of this study was to analyze the Pb heavy metals concentration in water culture and its concentration in holdfast and thallus of Gracilaria sp. In this research, determination of samples in each pond was taken on day 0 (before planting), 20 days (Initial Production) and on day 40th (Post/Harvest). The aquatic parameters include salinity, temperature, acidity (pH), and dissolved oxygen (DO). Quantitative determination of heavy metals on sample using Atomic Absorption Spectrophotometry (AAS). Determinations of heavy metal accumulation in organism using Bioconcentratin factor (BCF) and Translocation factor was used to calculate the Pb heavy metal translocation process from the base to the tip of Gracilaria sp. The results of the study showed the highest Pb concentration found in the second sampling age 20 days, in pond 1 with 7.61 ± 0.18 mg.kg-1, and pond 2 was 5.35 ± 0.09 mg.kg-1. This concentration has not exceeded the threshold value that might have an effect if more than 8.6 mg.kg-1. The highest Pb level at the holdfast of Gracilaria sp. found at age 0 days before planting, which is 3.38 ± 0.23 mg.kg-1 and decreases to post-harvest (age of 40 days) which is 0.84 ± 0.00 mg.kg-1. The Transaction Factor (TF) of Pb heavy metal value from holdfast to thallus is 1,015 thus Gracilaria sp. absorbs heavy metals in high concentrations at the beginning of planting and is able to release it again before harvest time. Keywords: Bioaccumulation, Gracillaria sp., Heavy Metal, Histological, Red Algae.

Dietary Rice Bran Plays A Significant Role in the Hepatoprotective Effect in Hypercholesterolemic Rats Yulianti Antula; Dolly Irnawati Neno; Arie Srihardyastutie; Chanif Mahdi
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Cholesterol is obtained through biosynthesis and diet. When a level of cholesterol is above the normal level, this condition caused hypercholesterolemia. Long-term administration of synthetic chemical drugs can cause liver damage. Therefore, alternative natural medication is needed. One of the alternatives that can be used is the rice bran (RB), which contains antioxidant and crude fiber. This study is aimed at finding out the potential utilization of RB on total cholesterol level, liver enzyme as aspartate aminotransferase (AST) and alanine aminotransferase (ALT), malondialdehyde (MDA), and the changes in liver tissue. This study uses five groups of rats: the negative control group, positive control group, and three therapy groups with the dosage of 270 mg kg-1, 540 mg kg-1, and 810 mg kg-1 of body weight. This study shows that therapy using RB can significantly decrease the cholesterol level, AST, ALT, and MDA (p<0.01). The total cholesterol level is 21%, AST and ALT activities can be reduced to 54% and 64%, the level of MDA reduced to 79% and can repair the liver tissue. This study shows that RB can be effectively used as hepatoprotective in rats with hypercholesterolemia. Keywords: AST and ALT, Hypercholesterolemia, Rice Bran, Total Cholesterol Level.

Phytochemicals and The Ability of Plantago major Linn. Extract to Inhibit The growth of Aeromonas hydrophila Annisa Farhana Dewi; Arief Prajitno; Ating Yuniarti
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Aeromonas hydrophila is a type of gram-negative bacteria that can harm humans and major animals that have poikilotherm properties such as fish and shrimp. These bacteria can cause haemorrhages in fish until death and causing fish farming production to failure. The use of medicinal plants has been trusted by people all over the world to overcome various disease problems, one of which is a disease caused by bacteria. Plantago major L. is known that able to inhibit the growth of both gram-positive and gram-negative bacteria. The purposes of this study were to identify the extracted compounds contained in Plantago major L., using FTIR and UV-VIS spectrophotometer and to evaluate antibacterial activity against A. hydrophila. The results of this study showed that crude extracts of Plantago major L. contained polar compounds such as phenols, flavonoids, saponins, and tannins. The presence of phenols and flavonoids was confirmed by the results of FTIR and UV-VIS. Furthermore, the crude extract of Plantago major L. significantly inhibited the growth of A. hydrophila (P <0.05).Keywords: Antibacterial, Co-culture, Extraction, FTIR, UV-VIS.

Potential of Dayak Onion (Eleutherine palmifolia (L) Merr) Extract As Antibacterial Against Pseudomonas fluorescens Immaria Fransira; Uun Yanuhar; Maftuch Maftuch
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Dayak onions (E. palmifolia (L) Merr) is a herb used as medicine and known to have antibacterial activity. This research aimed to evaluate antibacterial activity of Dayak onion (Eleutherine palmifolia (L) Merr) extract against fish pathogen Pseudomonas fluorescens. The method used in this study was extraction by maceration, UV-Vis spectrophotometry analysis on the extract and antibacterial activity test with Minimum Inhibitory Concentration (MIC) as well as disc diffusion test. The results of this study showed that the yield value was 8.87%. Based on UV-Vis analysis, it confirmed that extract of Dayak onion (Eleutherine palmifolia (L) Merr) contains predominantly flavonoids and phenols with its derivatives, that have antibacterial activity. The results of MIC and disc diffusion test showed potential antibacterial activity against P. fluorescens, as observed from the value of absorbance and inhibition zones that formed. Keywords: Antibacterial, Disc diffusion, Eleutherine palmifolia (L) Merr, MIC, Pseudomonas fluorescens.

Phytochemical Screening and Antimicrobial Activity of Roselle (Hibiscus sabdariffa L.) Flower Extract Against Aeromonas hydrophila Sitti Khairul Bariyyah; Arief Prajitno; Ating Yuniarti
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Medicinal plants as an antimicrobial agent may provide an alternative way to replace the use of antibiotics to control disease agents in aquaculture. Roselle flowers (Hibiscus sabdariffa L.) has been used in many sectors as a source of functional food, natural coloring agents as well as antimicrobial agents. The objectives of this study were to evaluate the phytochemical compound in methanolic extract of roselle flower and their antimicrobial activity against Aeromonas hydrophila. The phytochemical composition of roselle flower was evaluated using phytochemical screening and FTIR. While the antimicrobial activity was performed by using the disc diffusion agar and co-culture with A. hydrophila. The results of phytochemical screening confirmed the presence of alkaloids, flavonoids, saponins, steroids, triterpenoids, and tannins. The results of FTIR revealed that Roselle flower extract had the main phenolic compounds. The result of disc diffusion and co-culture method indicated that the roselle flower extract had antibacterial activity against A. hydrophila. This antibacterial activity depended on the concentration applied.Keywords: antibiotics, Co-culture, Disk diffusion, flavonoid, Hibiscus sp.

Effects of Monosodium Glutamate Oral Administration on LH and Testosterone Levels in Serum of Adult Male Rats (Rattus norvegicus) Riska Annisa; Moch. Sasmito Djati; Sri Rahayu
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

This study aims to determine the effect of monosodium glutamate on LH and testosterone levels in serum of adult male rats (Rattus norvegicus). A total of 12 male Wistar-strained rats divided into three groups with 4 rats per group. Control group was given distilled water, while M1 was given MSG at a dose of 4mg g-1 BW for 15 days and M2 was given MSG at a dose of 4 mg g-1BW for 45 days. All substances were given orally. At the end of the study, rats were sacrificed with a dislocation to draw blood from the heart. Then, the blood was incubated at room temperature to obtain serum. Serum was used to analyze LH and testosterone levels using the ELISA kit. Data were analyzed using one way ANOVA (p ≤ 0.05) with SPPS 16.0 for windows. The results showed that serum LH levels after MSG treatments for 45 days was significantly decreased compared with the control. The serum testosterone levels M2 was significantly difference with the control group (p ≤ 0.01). This study predicts that monosodium glutamate have a direct and indirect effect on male reproductive organs. Keywords: Luteinizing hormone, Monosodium glutamate, testosterone.

Growth and Development of Rhyzopertha dominica Fabricius (Coleoptera: Bostrichidae) on White, Red and Black Rice Dewi Fajarwati; Ludji Pantja Astuti; Toto Himawan
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Rhyzopertha dominica is a polyphagous stored pest to many grains including rice. R. dominica can easily spread from one place to another to infest stored products. The aim of this research was to know the influence of three grains types with different weight to R. dominica growth, development, net reproduction rate and gross reproduction rate on red, black and white rice. The research was conducted in Pest Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, University of Brawijaya Malang. The research result showed that there was an interaction among three grains types with different weight to the number of R. dominica larval, pupal, and newly adult emerged, while the number of eggs, weight of newly adult emerged, the reduction percentage of grain and phase of the egg, larval, pupal and the life cycle period of R. dominica were affected by different grains types factor. The R0value showed that on the next generation, number of female was increase 1.25 times from the previous generation on white rice, while on red and black rice the population was increase up to 2.63 and 2.49 times from the previous generation.Keywords: Growth and Development, Reproduction Rate, Rice, Rhyzopertha dominica.

The Effect of Monosodium L-Glutamate (MSG) Treatment for Short and Long Terms to The Semen Quality of Adult Male Rats Ivakhul Anzila; Agung Pramana Warih Marhendra; Sri Rahayu
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

This study was conducted to investigate the effect of MSG treatment for short and long term on the semen quality of adult male rats. Twelve male adult Wistar rats with 200-300g of body weight (BW) and 3-4 month of age were used in this study. The animals were divided randomly into 3 groups. M0 was used as a control, M1 and M2 were given with MSG 4mg/gBW for 15 andÂ 45 days respectively. The experimental animals were sacrificed on the days 16th (to M1 group) and 46th (to M0 and M2 groups). The epididymis was isolated and semen quality (motility, viability, concentration, and abnormality of sperm) was evaluated. The results showed motility and concentration of M1 and M2 were not significantly decreased compared to M0. MSG treatment also significantly reduced viability and increased abnormality of sperm. Analysis of sperm abnormality character shows that the use of long-term MSG caused a formation of the primary abnormality (round and double head sperm) and increased the secondary abnormality (bent neck, curve tail, coiled tail, headless, and tailless) compared to control. Conclusion, semen quality decreases with consumed MSG for the long term. For this reason, reconsidering the use of MSG as an enhancer for the teste of food is very important.Keywords: epididymis, Monosodium L-Glutamate, semen quality.

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Jehan Ramdani Hariyati

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Jawa timur

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

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Abstract

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Abstract

Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA