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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 11 Documents

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Search results for , issue "Vol. 9 No. 3 (2019)" : 11 Documents clear

The Potential Microbe Agent to Degrade Naphtalene and Phenanthrene in Dumai Oil Sludge : A Growth Response Test Hardianti, Alfi Rizca; Sawitri, Dita Widiyanti; Lusiana, Helga
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

This study aims to find the most appropriate indigenous bacteria as Naphthalene and Phenanthrene degrading agents carried out in the growth response test. This research is very important to find the best bacterial agent, which in the future can reduce the impacts of oil pollution, as well as from the presence of oil sludgeBacterial cultures added in the media as much as 5% (v / v), which had previously been measured OD660nm = 0.5. The growth response test was carried out by growing 20 mL of bacteria in AMS media which was added with PAH substrate, naphthalene or Phenanthrene in various concentrations: 200 ppm, 500 ppm, 800 ppm, and 1000 ppm. In the naphthalene substrate with 24-hour incubation, the best treatment was in isolates A with a concentration of 200 ppm. In the phenanthrene substrate 24 hours incubation, the best treatment was in isolate A at 200 ppm, and at 48 hours incubation. Bacterial isolates F in 24-hour incubation increased growth in all treatments and controls. The bacterial E isolate on the phenanthrene substrate at 24-hour incubation has increased growth, which occurs until the incubation time is 48 hours, both in the control and phenanthrene substrate. E Isolate is tolerant of increasing substrate concentrations up to 1000 ppm, both naphthalene and phenanthrene and has the best growth ability. It can be concluded that isolate E has the best growth ability and is tolerant of increasing substrate concentrations up to 1000 ppm. E isolates were designated as the best isolates of naphthalene and phenanthrene degradation.Keywords: Dumai, Growth Response, Naphthalene, Phenanthrene

CFGWC-PSO in Analyzing Factors Affecting the Spread of Dengue Fever in East Java Province Abdussamad, Siti Nurmardia; Astutik, Suci; Effendi, Achmad
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Fuzzy Geographically Weighted Clustering-Particle Swarm Optimization using Context Based Clustering (CFGWC-PSO) has been developed to clustering in factors influencing the spread of dengue fever in East Java Province. CFGWC-PSO method can overcome slow computing time problems in terms of iterations, and produce accurate data partition with stable. In this research, CFGWC-PSO applied to 11 variables from data on the causes of the spread of dengue fever in East Java Province in 2017. CFGWC-PSO using the FCM method to determine the context variable. Processing used the results of clustering with 2 clusters until 5 clusters. From the three validation index that used to find out the right number of clustering, two clusters gave better clustering results. CFGWC-PSO shows that all districts/cities in cluster 2 become dengue fever endemic areas that need to be considered by the East Java Provincial Government.Keywords: Context-Based Clustering, dengue hemorrhagic fever, Fuzzy Geographically Weighted Clustering-Particle Swarm Optimization.

The Response of Botanical Seeds of Trisula and Biru Lancor Shallot Varieties to Coconut Water Treatment Sudaryono, Tri
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

The use of botanical seeds for seedling cultivation has problems related to the number of produced tubers, where in general botanical seeds on average only produce 1-2 tubers. Another problem with the cultivation of shallots with botanical seeds as a source of seedlings is the low growth of seeds (germination). To overcome the problems that exist in botanical seeds, growth regulators are used. This study aimed to determine the effect of coconut water treatment on the growth and yield of Trisula and Biru Lancor shallot varieties from botanical seeds, carried out from April to August 2018 in Pelem Village in Pare Sub-District, Kediri Regency. The utilized method is soaking the shallot botanical seeds for 4 hours before sowing; the seeds were then sown and planted for a month before being moved to the field. After growing in the field, observations were carried out at 1-week intervals on vegetative and generative growth. Sampling was random, and data was analyzed with t-test/LSD at a level of 5%. The results showed that the botanical seeds of Trisula and Biru Lancor shallot varieties showed different responses to the treatment with young coconut water. The use of coconut water for the Trisula variety led to 70% of plants yielding more than six cloves, while the Biru Lancor variety only had 46.67%. The use of coconut water for the Trisula variety yielded a fresh tuber weight of 71.2 g per plant at harvest; if converted per hectare, the Trisula variety can yield approximately 32 tons of fresh tubers. Meanwhile, the Biru Lancor variety yielded a tuber weight of 57.4 g per plant at harvest, and if converted per hectare, the Biru Lancor variety can yield approximately 26 tons of fresh tubers.Keywords: Botanical seed, Coconut Water, Shallots

The Antigenotoxic Activity of Brown Seaweed (Sargassum sp.) Extract Against Total Erythrocyte and Micronuclei of Tilapia Oreochromis niloticus Exposed by Methomyl-Base Pesticide Kilawati, Yuni; Islamy, R Adharyan
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Pesticides are widely applied in the agriculture sector to protect crops and pest control. The poisonous substance of pesticides will affect all of the organisms, either target and non-target organisms. Fish can play the role of an indicator of genotoxic presence in aquatic environments. Polysaccharide extracts from sargassum have promising anti-genotoxic potential. This study aimed to analyze the anti-genotoxic activity of brown seaweed (Sargassum polycystum) methanol extract against erythrocyte and micronuclei of tilapia exposed by methomyl-base pesticide. Brown seaweed (Sargassum sp.) purchased from farmers in Sumenep Regency, Madura, East Java, then macerated using methanol 1: 3 (w / v) for 3x24 hours at room temperature. The phytochemical screening was including flavonoids, alkaloids, triterpenes, saponins, and tannins. Tilapia fish (TL ± 9-12cm) purchased from the Technical Application Unit of Freshwater Fish (UPT Perikanan Air Tawar), Sumberpasir, Malang, East Java. The result of this study showed that exposure of methomyl-based pesticides in the concentration of 4.015 ppm indicates the formation of micronuclei of 318.33 ‰. The increased concentration of extract treatment is directly proportional to the decrease of micronuclei. It means that sargassum extract can reduce the genotoxic effect on exposed tilapia by methomyl-based pesticides. The best concentration of Sargassum sp. extract that can reduce genotoxic was D (200 ppm). Keywords: Antigenotoxic, Extract, Methomyl, Pesticides, Sargassum sp., Tilapia.

Effect of Five Types Inert Dust to Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae) in Stored Rice Seeds Astuti, Ludji Pantja; Maula, Rohmatin; Rizali, Akhmad; Mario, Muhammad Bayu
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Rhyzopertha dominica (F.) is one of the primary pests in stored rice. Generally, pest control in the storage was done by spraying and fumigation using synthetic insecticide. The application of chemical insecticide using phosphine can cause resistant to insect pests and toxic to humans. Regarding the negative effect of insecticide application, the alternative control by using natural resources like inert dust is expected to be a good solution to control pests of stored products. This research was aimed to study the effectiveness of five inert dusts, which are: rice husks ash, volcanic ash, giant bamboo leaves ash, corncobs ash, coconut shells ash to adult mortality and the inhibition of population growth of R. dominica on stored rice seeds. The results showed that giant bamboo leaves at 8 g kg-1 caused 100% of mortality and faster than other inert dusts tested. Giant bamboo leaves and rice husks were more effective to suppress the number of eggs, larvae, pupae, and new adults (F1). Inert dust could decrease the hatchability of eggs, inhibit population growth, and decrease the weight of new adults (F1). The damage to the treated rice seeds was significantly lower than untreated rice seeds. Keywords: ash, control, inert dust, silica, stored product pest

The Effect of Moringa oleifera Leaves and VipAlbumin® on The Immune System of Diabetes Mellitus Balb/C Mice Model Almabrouk, Suheer Khalleefah; Dwi, Noviana; Rahayu, Sri; Rifaâ€™i, Muhaimin
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Diabetes Mellitus is known as a disease grown worldwide rapidly prevalent day by day. This disease caused by a chronic hyperglycemic condition and also glucose intolerance resulting from defects in insulin secretion, insulin action, or both. Moringa oleifera is one of the plants used in most developing countries for traditional medicine for treating diabetes. In this research, Moringa oleifera mixed with VipAlbumin®, taken from snakehead fish albumin. Albumin found as an antioxidant against ROS. This research aimed to evaluate the effect of Moringa oleifera mixed with VipAlbumin® supplement towards the profile changing of T-cell, (CD4+ CD8+) B220+, CD4+ IFN-γ, and TNF-α. The experiments were done by dividing Balb/c mice into five groups and induced with 100 mg.BW-1 STZ. Afterward, the mix of Moringa oleifera and VipAlbumin® orally administrated into five different doses. Negative control contains of healthy mice, Positive dose administrated with 145 mg.kg bw-1 STZ, Dose 1 of 100 mg.kg bw-1 (Mo) + 416.25 mg.kg bw-1 (A), Dose 2 of 150 mg.kg bw-1 (Mo) + 208.15 mg.kg bw-1 (A), Dose 3 of 50 mg.kg bw-1 (Mo) + 624.375 mg.kg bw-1 (A). One way ANOVA was applied to analyze the data with p-value 0.05% and combined with Tukey test using SPSS version 16 for Windows. The results showed that a relative number of CD4 and CD8 T cells decreased in dose 3 of Moringa oleifera and Albumin, as well as B220 in dose 3 gave a significant decreased compared to healthy mice (p<0.05). The inflammation showed decreasing after treatment with dose 3 of Moringa oleifera and albumin extract. Taken together that proinflammatory cytokines decreased after treatment compared to a positive group. Keywords: Albumin, Inflammation, Moringa oleifera, T cells.

Molecular Detection, Histopathology, and Scanning Electron Microscopy of Myxobolus koi Infecting Cyprinus carpio Koi Soelistyoadi, Rachmat Noer; Yanuhar, Uun; Maftuch, Maftuch
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Myxobolus koi is a type of parasite that infects many freshwater fish through myxospores, resulting in a disease called myxobolosis. This research aimed is to investigate the clinical symptoms that occur in the gills of infected Koi carp (C. carpio). The utilized methods in this study are molecular detection, histopathology, and Scanning Electron Microscopy (SEM). Koi carp samples that are suspected to be infected with Myxobolus koi show several clinical signs, such as swollen and pale-colored gills and gill covers (opercula) that do not close completely, as well as white spots that appear on the edge of the lamella. The results of this study showed that through histological observations, there were changes characterized by hyperplasia of the primary lamella cartilage that envelops parasitic cysts and the encapsulation process that surrounds the cysts by gill cartilage in infected fish;SEM showed oval and elongated spores of a size of ± 12 µm (800x). TheDNA template from gill tissues in clinically infected and uninfected fish was examined byPCR testing with primers ERB 1 (Forward) and ERB 10 (Reverse); the results of electrophoresis in infected fish were detected at 2000 bp. Keywords: Cyprinus carpio, Histopathology, Molecular Detection, Myxobolus koi, Parasite, SEM.

Analysis Activity of Elephantopus scaber Leaves Extract Against Quantitative Changes of Lymphocytes Cells in BALB/c Mice After Induction of DMBA and Estrogen Rochmatika, Lailiyavina; Widyarti, Sri; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

The carcinogenic compounds such as DMBA known for having the ability to promote the DNA damage that affects the growth regulator genes. Therefore, uncontrolled cell growth will occur and change the immune system. Elephantopus scaber extract is considered as an alternative way of handling breast cancer since it capable to affect the work of the immune system. Hence, this study aims to determine the effect of E. scaber extract on quantitative changes of CD4+, CD8+ T cells and proinflammatory cytokines CD4+IFNγ+ and CD4+TNF-α+ after induction of DMBA and estrogen. In vivo experiments were carried out by using 5-6 weeks old of female mice BALB/c strain. The extract of E. scaber leaf powder was obtained from Material Medica Batu, Malang. The experimental animals were divided into five groups such as negative control group (K-), positive control group (K+), mice treated with E. scaber extract for a week (group 1), mice treated with E. scaber extract for 2 weeks (group 2), and mice treated with E. scaber extract for 3 weeks (group 3). Induction of DMBA and estrogen was conducted by injection of DMBA (7.12 dimetylbenz (α) anthracene) 0.56 mg.kgBW-1 and estradiol hormone 0.0504 mg.kgBW-1. Mice were treated with E. scaber extract with a dose of 50 mg.kgBW-1. Lymphocytes were isolated from spleen and observed the CD4+, CD8+ T cells and proinflammatory cytokines CD4+IFN-γ+ and CD4+TNFα+ T cells through flow cytometry analysis. One-way ANOVA (p <0.05) and SPSS were used to analyze this data. The results showed that the induction of DMBA and estradiol hormone affected mice fur loss. It also decreased the immune system by lowering the number of CD4 and CD8 T cells. However, E. scaber extracts increased the number of pro-inflammatory cytokines TNF-α. Furthermore, the DMBA and estradiol induction also promoted the number of CD4+IFN-γ+ in the first week, but decreased the number of CD4+IFN-γ+ at weeks 2 and 3, then increase the number of CD4+IFN-γ+. So, the treatment of E. scaber extracts demonstrated the ability to restore the homeostasis of the immune system in the first and second weeks. Keywords: CD4+, CD8+, DMBA, estrogen, E.scaber, IFN-γ+, TNF-α+

Effect of Pb and Cd Elicitors on Growth and Content of Vetiver Oil Adventitious Root In Vitro of Vetiver (Vetiveria zizanioides L. Nash) Ivani, Trecy; Widoretno, Wahyu
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

This research aimed to evaluate the effect of Pb and Cd elicitors on root growth and essential oil content of vetiver oil on the root culture of vetiver (Vetiveria zizanioides L. Nash). Roots were induced by culturing of the shoot on MS media supplemented with 0.1 mg.L-1 kinetin and 7 mg.L-1 NAA. Elicitation was done by culturing roots on MS media with 0.1 mg.L-1 kinetin and 7 mg.L-1 NAA + 0.1 mM Pb or Cd. Roots then observed for fresh weight, dry weight, number, and root length, and vetiver oil contents were analyzed using GC-MS. The addition of Pb and Cd heavy metal elicitor affected the formation and the content of vetiver oil compounds of root culture. Pb elicitor increased the number of roots, while Cd elicitor increased root length. However, the addition of Pb and Cd elicitor on culture media decreased the fresh weight and root dry weight. Fifty eight vetiver oil compounds in adventitious roots of vetiver plants were identified. The main compounds of vetiver oil in roots culture were Khusimone (6.94%), Khusimol (6.05%), Khusimene (4.85%), α Vetivone (3.70%), β Vetivone (3, 53%), Vetiverol (3.22%), Prezizaene (2.35%), and Zizaene (1.91%). Elicitation with Pb and Cd increased the composition of the main compounds of vetiver oil. Cd elicitor increased the composition of the main compound vetiver oil higher than the Pb elicitor. Keywords: adventives root, elicitor, in vitro, Pb and Cd, vetiver oil

Phytochemical Screening by FTIR Spectroscopic Analysis and Antibacterial Activity of Sesbania grandiflora Ndahawali, Suryaningsih; Andayani, Sri; Hardoko, Hardoko
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Sesbania grandiflora is a medicinal plant that has been used by many Indonesian people. They have the ability as antibacterial because they contain bioactive compounds that inhibit bacterial growth. Sesbania grandiflora also contains other active compounds such as tannins, saponins, steroids, flavonoids, and terpenoids. This study aims to determine the antibacterial activity of extracts, n-hexane fraction, ethyl acetate fraction and water fraction of Sesbania grandiflora against Edwardsiella tarda using the well diffusion method in different concentration (75 ppm, 150 ppm dan 225 ppm), and knowing the active compounds of FTIR absorption bands. MIC (minimum inhibitory concentration) value of extract, n-hexane, ethyl acetate, and water fractions respectively was 0.51 %, 1.11 %, 0.97 %, dan 0.79 %. InIibitory zone diameter of the n-hexane fraction is most active against Edwardsiella tarda bacteria. The results of FTIR identification showed that n-hexane fraction contained flavonoids and terpenoids, as seen from absorption bands that function as antibacterial compounds.Keywords: antibacterial, Edwardsiella tarda, FTIR, Phytochemical Sesbania grandiflora.

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Home Page

OAI Link

Editorial Team

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Reviewer

Google Scholar

Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA