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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Published by Universitas Gadjah Mada
ISSN : 01261312     EISSN : 23563931     DOI : http://dx.doi.org/10.19106/JMedSci005303202101
Core Subject : Science,
Immunology & microbiology Neuroscience
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 2,170 Documents
 108   109   110   111   112 
Collagen synthesis on ultraviolet A irradiated human skin fibroblast treated with insulin Febrina Rismauli Panggabean Satiti Retno Pudjiati Yohanes Widodo Wirohadidjojo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 43, No 01 (2011)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Ultraviolet (UV) irradiation from the sun can stimulate premature skin aging because UV irradiation inhibits collagensynthesis, promotes collagen degradation and inhibits fibroblast proliferation. Insulin is capable to stimulate fibroblastgenes collagen expression, DNA synthesis, and collagen synthesis. The effect of insulin in reducing collagen synthesisamong repeated-UVA irradiation on human skin fibroblast has never been studied. This study aims to investigate theeffect of insulin in collagen synthesis among repeated-UVA irradiation on normal human skin fibroblast. To asses thecollagen synthesis collagen degradation, collagen deposition and fibroblast proliferationweremeasured. Experimentalstudy was performed among passage 3 of fibroblast which was isolated from a circumcised skin of a 6-year-old boy.Fibroblasts were irradiated with 3 repeated exposurewith total cumulative dose 9000 mJ/cm2 and treated withinsulin 0.5; 1; 2 μg/mL and placebo. Cellswere then incubated for 48 hours, collagen degradation, collagen depositionand fibroblast proliferation were read colorimetric by using Spectroscopy 550 nm. The effect of insulin 0.5; 1 and2 ìg/mL in collagen synthesis among repeated-UVA irradiation on normal human skin fibroblast with cumulative dose9000 mJ/cm2 was not capable to reduce collagen degradation, nor capable to increase collagen and fibroblastproliferation. Insulin dose 0,5 μg/ml-2 μg/ml among repeated-UVA irradiation on normal human skin fibroblast wasnot capable to increase collagen synthesis.Key words: photoaging-DNA synthesis-proliferasion-aging process-gene expression
The effect of megadosis of iron consumption on the absorption of zinc and copper in rat (Rattus rattus) determined in situ Sukarti Moeljopawiro Sukarti Moeljopawiro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 30, No 03 (1998)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Oral administration of iron pill must be at an accurate dosis because high consumption of iron could impair the absorption of other minerals especially bivalent metals. Therefore, the objective of this study was to evaluate the effect of megadosis consumption of iron on the absorption of zinc and copper. The inorganic minerals were used in this study. The amount of mineral absorption was determined in situ. Forty male weanling rats were used in, this study. Rats were divided into 8 groups of 5, two groups for each treatment. Four ratios of Fe, Zn and Cu were used in this study (Fe : Zn : Cu = 5 : 1 : 1; 10 : 1 : 1; 20 : 1 : 1 and 50 ; 1 : 1). The amount of mineral absorption was determined using in vivo intestinal perfusion, and two perfusion rates were used 0.5 ml/minute and 0.33 ml/minute. It was found that high iron consumption (50 : 1 : 1) could impair the zinc absorption at perfusion rate of 0.33 ml/minute and no zinc absorption occur at perfusion rate of 0.5 ml/minute. However, this high iron consumption did not influence the copper absorption both at perfusion rate of 0.5 ml/minute and 0.33 ml/minute. It can be concluded that high consumption of iron could impair the zinc absorption but not copper absorption.Key words : iron - zinc - copper - mineral absorption - in situ perfusion
Developing and promoting community participation in the control of dengue haemorrhagic fever vectors Sugeng Juwono Mardihusodo Sugeng Juwono Mardihusodo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 19, No 01 (1987)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Dengue haemorrhagic fever (DHF) is caused by dengue virus and transmitted by Aedes aegypei and A e. albopictus mosquitoes. The absence of causative drugs and protective vaccines, therefore, indicates vector control measures as the only alternative for the disease abatement. Various methods of dengue vector control and of developing and promoting community participation in such activities are discussed. Key Words: dengue haemorrhagic fever — Aedes aegypti — Breteau Index — environmental sanitation — Rural Improvement Programme
Peranan Diagnostik Teknik Imunofluoresensi Dalam Dermatologi Hardyanto Hardyanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 12, No 03 (1980)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Diagnostic procedures by means of immunofluorescence techniques have been greatly used in investigations of some diseases which have immunologic background. These tests are now accepted as routine diagnostic procedures in dermatology in advanced countries beside electron microscopy. The main purpose of this paper is to summarize intelligibly immunofluorescence results obtained so far in various skin disorders. The methods of application can be divided into direct and indirect method.The basic principle, technical procedures, some problems and application of immunofluorescence on various skin diseases are discussed.Key Words: immunofluorescence techniques - direct and indirect immunofluorescence -- antigen-antibody tracing - immunologic skin diseases - dermatoses
Drug use study for acute respiratory infection in children under 10 years of age Iwan Dwiprahasto Iwan Dwiprahasto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 37, No 04 (2005)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Acute respiratory infection (ARI) is the commonest illness in children and the leading cause of morbidity and mortality in many developing countries. It comprises approximately 50 % of all illness in children under five years. Even though usually viral in origin and of a self-limiting nature, various study indicate that antibiotic prescribing for ARI is inappropriately high.Objective: This study was aimed to assess general practitioners' (GPs) prescribing pattern for acute respiratory infection in children seen in private practices.Method: This study is carried out in a cross sectional study design. Participants were GPs and pharmacies in Yogyakarta Special Province. Data on patient and measure of respiratory rate were obtained from GPs' office and data on prescribing were searched at all pharmacies in the province. Result: All pharmacies and 92.27% of GPs participated in the study. The average number of drug item prescribed for children with ARI was 3.74. The most widely used drugs for ARI were antibiotics followed by paracetamol, ibuprofen, chlorpheniramine maleate, phenobarbital, vitamin-C, dextromethorphan, glyceryl guaiacolate, dexamethasone, prednisone, vitamin-B6, ephedrine HCI, vitamin BI, and vitamin B2. Medication error was commonly found in the study. More than 60% children with ARI received drugs in either overdose (27.51%) or under dose (41.12%). Wrong preparation were detected in 24.07% prescriptions. It was also found that more than one fifth of children with ARI received drugs in wrong frequency of administration. Risk predictor for prescribing antibiotics were fever, abnormal respiratory rate, and duration of common cold symptom of more than 2 days.Conclusion: ARI in children was often treated unnecessarily. Medication error was also commonly found in the treatment of children with ARI visiting GPs. Physicians must be encouraged to improve their prescribing practices by always catching up with current best research evidence. Key words: acute respiratory infection - inappropriate prescribing - medication error - general practitioners - emergence of resistance
Comparison of serum aminotransferase between gas and gasless laparoscopy cholecystectomy Imam Sofii Hendro Wartatmo Agus Barmawi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 42, No 01 (2010)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Carbondioxide (CO2) insufflations in laparoscopy with gas will increase intraabdominal pressure that influences thehemodynamic, lungs, and kidneys. One of important hemodynamic changes is temporary reduction of hepatic bloodflowbecause of pneumoperitoneum. Pressure caused by pneumoperitoneumcan influence ischemia degree of hepaticcell and cause hepatic enzymes increase. Enzyme that includes in hepatic enzyme is aminotransferase,which consistsof: transaminase (AST) or glutamic oxaloacetic transaminase serum and alanine transaminase (ALT) or glutamicpyruvic transaminase serum. Laparoscopy method by lifting abdomen wall (gasless laparoscopy) without CO2insufflations can decrease the damaging effects of high intraabdominal pressure. This research was an experimentalresearch with single blind randomized clinical trial (RCT) plan, with observation of symptomatic cholelithiasis patientswho underwent cholecystectomy laparoscopic cholecystectomy with gas or CO2 (pneumoperitoneum) or withoutgas (gasless). Hepatic function tests were then held at 24 hours and 72 hours after operation. Research subjectswere symptomatic cholelithiasis patients who fulfilled inclusion and exclusion criteria. Samples needed were 24people in each group. The independent variable was patients with symptomatic cholelithiasis who underwentcholecystectomy laparoscoped with gas compared to those being cholecystectomy laparoscoped without gas. Thedependent variable was aminotransferase enzyme value before operation, and 24, 72 hours postoperation. The datawere analyzed using Kolmogorov Smirnov, independent t-test, pair t-test, and MannWhitney test. It was obtained21 cases for men (43.75%), 27 cases for women (56.25%). The average age of the group laparoscopy with gaswas 47.16±10.76 years old and the group laparoscopy without gas was 45.3±11.48 years old (p>0,05). Theaverage values of AST and ALT 24 hours postoperation of the group laparoscopy without gas were 21.9±7.6 U/L(increase 24%) and 26.3±5.2 U/L (increase 46%) compared to 65.8±18.4 U/L (increase 206%) and 62.8±14.3U/L (increase 280%) in the group laparoscopy with gas (p< 0,05). The average values of AST and ALT 72 hourspostoperation of the group laparoscopywithout gaswere 24.7±8.3 U/L (increase 33%) and 28.9±7.3 U/L (increase17%) compared to 71,5±28,6 U/L (increase 250%) and 75.8±16.9 U/L (increase 360%) in the group laparoscopywith gas (p< 0,05). In conclusion, there were significantly increases of serum aminotransferase values (AST andALT) in cholecystectomy laparoscopy with gas compared to in cholecystectomy laparoscopy without gas.Key words: cholecystectomy laparoscopy – pneumoperitoneum - gasless – serum aminotransferase
Corpus delicti di dalam bidang ilmu kedokteran kehakiman Nawawi Nawawi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 5, No 03 (1973)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

keywords: visum et repertum, corpus delicti
The diagnostic value of fine needle aspiration biopsy and ultrasonography on thyroid nodule in Dr. Sardjito General Hospital, Yogyakarta Wicaksono Probowoso Ishandono Dachlan
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 02 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

As the prevalence of thyroid cancer increases, a diagnostic method that can identify malignancy that warrants further surgical treatment is needed. Fine needle aspiration biopsy (FNAB) and ultrasonography (USG) are preoperative test for diagnosing thyroid tumor. This research aimed to calculate the diagnostic value of FNAB and USG on thyroid nodule patients in Dr. Sardjito General Hospital, Yogyakarta. This was a diagnostic test study using a retrospective designusing data from medical records of patients with thyroid nodules from January 2006 to December 2010. The diagnositic value of FNAB and USG including sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy were calculated and compared with histopathological examination as the gold standard. Ninety medical records data of patients consisting of 71 females and 19 males who were diagnosed thyroid nodule with FNAB or USG before surgery were evaluated in this study. The results of FNAB of 90 patients were as follows: 54 (60.0%) benign, 6 (6.7%) malignant, and 30 (33.3%) follicular neoplasm. Meanwhile, the results USG of 90 patients were as follows: 38 (42.2%) benign, 15 (16.7%) malignant and 37 (41.1%) non determined. The diagnostic value of FNAB was described as follows: sensitivity of50.0%, specificity of 100%, PPV of 100%, NPV of 50.0% and an accuracy of 60.0%, whereas the diagnostic value of USG was described as follows: sensitivity of 81.8%, specificity of87.5%, PPV 60%, NPV 94.5% and accuracy of 50.0%. In conclusion, the diagnostic value of FNAB and USG in stablishing diagnosis of thyroid nodule is still low. The FNAB has higher accuracy compared to USG for diagnosing thyroid nodules.Keywords: diagnostic value - fine needle aspiration - ultrasonography - thyroid nodule - histopathological examination
The effect of combined timolol maleat and p/locarpine acetazolamide on the decrease of intraocular pressure in the treatment of primary glaucoma Sutrisno Sutrisno
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 29, No 03 (1997)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

The study was aimed to compare the effects of combination of timolol maleat 0.5% and pilocarpine 2% to acetazolamide 250mg in the treatment of a primary glaucoma. We studied 13 patients with open angle glaucomas and 11 patients with closed angle glaucomas. This study was carried out in a randomized double blind clinical trial. The patients were divided into two groups, A and B. Group A consisted of patients treated with 250 mg acetazolamide t.i.d. and the group B was treated with combination of 0.5% timolol maleat b.i.d. and 2 % pilocarpine q.i.d. This one-day treatment was stopped and seven days later the treatment was interchanged between the two groups. The intraocular pressures were measured just before and two hours after the treatment. The mean decrease of 10P in group A was 17.042 ± 14.212mmHg and that of group B was 17.873 ± 9.005 mmHg. There was statistically no significant difference (p=0.804) between two groups. The mean decrease of 10P in primary open angle glaucoma in group A was 8.685 ± 6.389 mmHg and that of group B was 15.054 ± 5.994 mmHg. Statistically there was significant difference (p = 0.014). The mean decrease of 10P In primary closed angle glaucoma in group A was 26.918 ± 14.748mmHg and that of group B was 21.205 ± 10.993 mmHg. Statistically there was no significant difference (p = 0.3162). In conclusion, there was no significant difference between the use of acetazolamide 250mg q.i.d. the combination of timolol maleat 0.5% b.i.d. and pilocarpine 2% q.i.d. in reducing the 10P of primary glaucoma.Key words: primary glaucoma - lop decrease - acetazolamide - timolol maleat - pilocarpine.
The effect of topically extravirgin olive oil on the UVB-induced immunosuppression Vohanes Widodo Wirohadidjojo AriefBudiyanto, Irianiwati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 03 (2009)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: UVBradiation may act as an immunosuppressive agent through Langernans cells (LCs)depletion correlated with cyclobuthane pyrimidine dimer (CPD), as the most mutagenic photoproducts. Other studies showed that olive oil can prevent various human cancers, which are defect of immune-surveillance. The effect of olive oil in the UVBinduced LCs depletion is still unclear. Objective: To discover the topical effect of extravirgin-olive-oil in the LCs depletion. Methods: A simple experimental study was performed on foreskins collected from 8 circumcised boys. Each of them was cut into 3 pieces of 0.5 cm2, grouped randomly to: (Olive oil+ UVB)group, (placebo + UVB)group, and unirradiated group. They were cultured in DMEM for 24 hours, irradiated with 100mJs/cm2 UVB, and olive oil or placebo were then applied immediately after irradiation. All specimens were fixed in buffered-formalin, paraffin blocked, and cut to be 2 slides, and each of them was stained with anti-CD 1a or anti-CPD antibody. The pixels of AEC signats were digitally counted based on Photoshop's selected color histogram.The pixel means of various groups were analyzed by Kruskal-Wallis test and post hoc analysis by Mann Whitney test. Results: Olive oil treated groups showed a very significant (P

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