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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Published by Universitas Gadjah Mada
ISSN : 01261312     EISSN : 23563931     DOI : http://dx.doi.org/10.19106/JMedSci005303202101
Core Subject : Science,
Immunology & microbiology Neuroscience
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 2,170 Documents
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The percentage of macrophage numbers in rat model of sciatic nerve crush injury Satrio Wicaksono; Muhammad Ghufron; Rina Susilowati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 01 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2044.327 KB) | DOI: 10.19106/JMedSci004701201501

Abstract

ABSTRACTExcessive accumulation of macrophages in sciatic nerve fascicles inhibits regeneration of peripheral nerves. The aim of this study is to determine the percentage of the macrophages inside and outside of the fascicles at the proximal, at the site of injury and at the distal segment of rat model of sciatic nerve crush injury. Thirty male 3 months age Wistar rats of 200-230 g were divided into sham-operation group and crush injury group. Termination was performed on day 3, 7, and 14 after crush injury. Immunohistochemical examination was done using anti CD68 antibody. Counting of immunopositive and immunonegative cells was done on three representative fields for extrafascicular and intrafascicular area of proximal, injury and distal segments. The data was presented as percentage of immunopositive cells. The percentage of the macrophages was significantly increased in crush injury group compared to the sham-operated group in all segments of the peripheral nerves. While the percentage of macrophages outside fascicle in all segments of sciatic nerve and within the fascicle in the proximal segment reached its peak on day 3, the percentage of macrophages within the fascicles at the site of injury and distal segments reached the peak later at day 7. In conclusions, accumulation of macrophages outside the nerve fascicles occurs at the beginning of the injury, and then followed later by the accumulation of macrophages within nerve fascicles
Pregnancy after unilateral operation an androblasicuna Mochamad Anwar Mochamad Anwar
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 24, No 04 (1992)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Androblastoma is a rare semimalignant ovarian tumor which occurs most frequently during the reproductive years and is assumed to arise from sexually ambivalent cells noted in the ovary of the 6-7 weeks embryo or to be of teratoid origin. The excessive secretion of testosteron will occur and lead to masculinism, while androgens will suppress normal ovarian function and cause the sign of virilization. A 3-years subferdIc case was a 27 year-old-woman with typical syndrome of androblastoma manifested by virilization and defeminization. The testosteron concentration was 700 ng/dl, prolactin: 8,4 p.g/1, Barr body drumstick (+), while the chromosomal analysis indicated female genotype (46 XX). Simple oophorcctomy was done, the size of the left ovarian tumor was 9 x 7 x 8 cm, encapsulated and there was no adhesion. The uterus and adnexa were normal. The pathological result was malignant. Alkeran cytostatic treatment was given in 5 series of 12,5 mg orally. However, the pregnancy occurred during the treatment. The patient was then managed carefully as a routine procedure without and-cancer drugs anymore. The baby was born normally without any congenital anomaly. The signs of either virilism or masculinism decreased. Hormonal examination showed a normal level of testosteron.Key Words: infertility - androblastoma - cytostatics - ovarian tumor - virilization
Gadjah Mada Stroke Algorithm - Development and validity for distAngushing intracerebral hemorrhagic stroke with acute ischemic stroke or infarction stroke Rusdi Lamsudin Rusdi Lamsudin
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 28, No 04 (1996)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

A prospective study on 229 acute stroke patients was conducted to develop a Gadjah Mada Stroke Algorithm (GMSA). This algorithm was proposed as a clinical strategy for distinguishing intracerebral haemorrhage from acute ischaemic or infarction stroke after onset of stroke in 3 hospitals in Yogyakarta from 16th December 1989 until 15th November 1991. The following investigations have been made: (a) interobserver reliability for questionnaire and clinical examination of stroke patients, (b) interobserver reliability in the interpretation of CT-Scans of stroke patients, (c) validity of every clinical symptom against CT-Scans to define intracerebral haemorrhagic stroke, and (d) validity of seven multiple parallel tests against CT-Scans to define intracerebral haemorrhagic stroke. The GMSA was developed by one of seven multiparallel tests which has the highest validity. A multivariate statistical analysis and validate study showed that decreasing consciousness, headache, and Babinski's reflex at the onset are significantly related to intracerebral haemorrhagic stroke. This study showed that the GMSA was reliable and valid for distinguishing intracerebral haemorrhagic stroke from acute ischaemic or infarction stroke.Key words: Gadjah Mada stroke algorithm - diagnostic test - intracerebral haemorrhagic stroke
Age-Related Variation On Somatotypes Of Javanese People In Y Ogyakarta Province Nani Trilusiana Rahmawati Janatin Astuti Kumi Ashizawa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 04 (2008)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Body form has been related to a variety of behavioral, occupational, health, and physical performance variables, and somatotype is an effective technique for the study of anthropometric variations, which possess constantly recognizable characteristics and are the functional end products of the whole genetic and the developmental complex.Objective: The objective of this cross-sectional study was to describe age-related variations of somatotype in a cross sectional sample of healthy adult and elderly subjects from Yogyakarta Province Indonesia, and to promote the use of technique for the study of somatotype variations in aged populations.Methods: The study groups, consisted of 426 healthy Javanese people (185 males and 241 females) of age 18 - 90 years. The men and women samples were further divided into four groups: 18 - 20 years, 21 - 40 years, 41 - 60 years, and 61 - 90 years. All subjects were born and resident in Yogyakarta Province. Heath-Carter somatotypes were determined for all subjects.Result: The height and weight are significantly greater in males than in females. There was the relationship between age and somatotype component. The mean somatotype of the overall Javanese sample (18-90 years) is 3.0 - 3.2 - 2.9 or center type (SO: 1.2 - 1.3 - 1.3) in males and 4.4 - 3.3 - 2.2 or mesoendomorphic type (SO: 1.2 - 1.5 - 1.3) in females.Conclusion: In general, endomorphy and ectomorphy showed a week and even negative correlation with age group, while mesomorphy show a low but positive correlation. Significant sex differences in somatotype appeared only in endomorphy where females were higher than males. Javanese males somatotypes were less endomorphic and ectomorphic, but more ectomorphic than Javanese females.Key words: somatotype component - body size - variation - Javanese people
Anatomi Untuk Peningkatan Kualitas Hidup T. Jacob T. Jacob
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 21, No 01 (1989)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

The article describes the various divisions of anatomy made according to the need in facing a particular problem, among others the cla.4ification in functional systems and in levels of the living systems. Since anatomy is determined by both genetics and environment, and the total man also depends on his anatomy, efforts to improve the quality of life necessarily involves improvement in genetic and environmental qualities. The quality of life can thus be studied in terms of the functional anatomical systems and the anatomy at different levels of the living systems.There are many indicators developed to measure the quality of life. Mention is made about demographic indicators, socioeconomic indicators, sociomedical indicators, sociodental indices, biocultural indices and biomedical indices. Indicators based on purely anatomical factors are described, such as body height and weight, growth norms, anthropological measurements, body build and body composition, and biological rhythm.Other approaches could be developed by using vertical and horizontal interrelations of the discipline to study the quality of life from the vantage point of anatomy.Key Words: anatomy -- quality of life -- health indicators -- demographic factors -- height-weight indices
Ilmu kedokteran dan kurrirulum pendidikan tinggi farmasi T. Jacob T. Jacob
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 9, No 02 (1977)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

keywords: kurikulum pendidikan tinggi, pelayanan kesehatan, penelitian, ilmu kedokteran, farmasi
Role of Vitamin C on the viabilitys of the keratinocyte and HeLa cell with irradiation of UVB ray. Iryani Andamari Iryani Andamari
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 4 (2004)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background:Ultraviolet B (UVB) exposure is one of the external factors which can cause reduction of cell viabillity through photochemistry reaction and can be managed using antioxydant. Failures of DNA repair and apoptosis have some important roles in photocarcinogenesis.Objective: This research is aimed to know the effect of vitamin C on the viability of UVB irradiated keratinocytes and HeLa cells.Method: This research employed a simple experimental method. Two groups of cells used in this research were: Group I normal foreskin keratinocytes passage III, and Group II HeLa cells. Each group was divided into 25 sub-groups consisting of 2x104 cells each, treated with vitamin C at 0, 6, 12, 40, 200 µg/ml concentrations, and UVB irradiated (Phillips UVB TL4OW/12RS) at 0, 200, 400, 800, 1600 mJ/cm2 intensities. Each treatment was done in quintet. Viable cells were determined based on formazan blue reaction using ELISA-reader 550 nm 24 hours after treatment. The results were analyzed by independent t-test, one-way ANOVA, and multivariate analysis using three-way analysis of variance,Result: Vitamin C at 6, 12, 40, and 200 ug/m1 concentrations significantly increased normal keratinocyte and HeLa cells viability which were not irradiated by UVB, statistically significant, compared to normal keratinocyte and HeLa cells which were not treated with vitamin C. HeLa cells, which were irradiated by UVB at 200, 400, and 800, 1600 mJ/cm2 intensities and treated with vitamin C at 6, 12, 40 and 200 ug/ ml concentrations, statistically significant in decreasing cell viability.Conclusion: Vitamin C at 200 µg/ml concentration, which was given to normal keratinocytes irradiated by UVB at 200, 400, 800, and 1600 mj/cm2 intensities used in this research showed its protection effect, thus enhancing the viability of the cell. Vitamin C 6, 12, 40 ug/ml, which were irradiated with UVB at 200, 400, 800, and 1600 mJ/cm2 intensities, did not show protection effect. HeLa cells were less resistant than normal keratinocytes to UVB irradiation at 200, 400, 800, and 1600 mj/cm2 intensities.Key words: vitamin-C, HeLa cell, keratinocyte, cell viability, UVB
Severe atopic dermatitis with xerophthalmia in children: A case report Sumadiono Sumadiono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 33, No 04 (2001)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

The management of the atopic dermatitis is not easy and in the severe cases it is a chalenge for the physician. It was reported a 6 year and 3 month old boy with severe atopic dermatitis with erythroderma, secondary infection, marasmic type of severe malnutrition with xerophthalmia, suspected iron defeciency anemia, acute gastroenteritis and miliary tuberculosis. The child had suffered from this disease since he was 3 months old, spreading and becoming worse. The clinical manifestations were redness and black appearance of the skin, excoriation and cast in the skin, some pats of the skin chaping and watery, there was a lacerated wound around his nose. There were many relatives with atopic history in his family tree. The patient had allergy to some medicines (ampicillin and sulfa), and also to many kinds of food. There was eosinophilia in the peripheral blood. The patient had been treated with topical skin medicine, systemic antihistamin/corticosteroid, anti tuberculosis, antibiotics, and local treatment for his eyes, but no improvement. According to the discussion between pediatricians and dermatologists the patient was suffered from severe atopic dermatitis, and was suspected had another disease (geno dermatitis). It is important to avoid drugs that had triggerred allergy before. The limitation of the diet had caused severe malnutrtion. Vitamin A had to be given early. Second generation antihistamine was actually needed to be given early and for several months. It was necessary to do many examinations to establish more exact diagnosis, but the patient died before the examinations were done.Keywords : Atopi dermatitis in children - eosinophil - marasmus xerophthalmia - treatment
Effectiveness of subconjunctival mitomycin-C compared with subconjunctival triamcinolon acetonide on the recurrence of progresive primary pterygium which underwent Mc Reynolds method Donny W Chandra Agus Supartoto, Angela Nurini Agni
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 04 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: The main problem in the management of pterygium is how to diminish the recurrence rateafter surgical treatment. Mitomycin-C an antineoplastic, antifibrotic has been used to prevent recurrencerate of pterygium after excision, however, it correlated with some complications. Subconjunctivaladministration before excision has been proposed to avoid them.Objective: To know the effectivity of subconjunctival of mitomycin-C to decrease the recurrence rate ofprogresive primary pterigyum after Mc Reynold method compared with subconjunctival triamcinolonacetonide.Materials and methods: Randomized clinical trial of 41 progresive primary pterygium in Dr Sardjito Hospitaland Dr Yap Eye Hospital. They were randomly assigned to receive subconjunctival 0.1 ml triamcinolonacetonide or 0.1 ml mitomycin-C and underwent pterygium excision one week later using Mc Reynoldmethod. The follow up period was 6 months to detect any recurrence and complication of the drugsResults: The recurrence rate after subconjunctival mitomycin-C and triamcinolon acetonide was 4.7% and25%, respectively, however the different was not statistically significant (p=0.67). There were nostatistical difference in conjunctival hiperemia, lacrimation and granulation. Blepharospasm was significantlydifferent in seven days. The pain after injection and after excision was statistically different between twotwo groups.Conclusion: The recurrence rate of progressive primary pterygium in triamcinolon acetonide group washigher than mitomycin-C group but there was no statistical difference.Key words : Progressive primary pterygium – mitomycin-C – triamcinolon acetonide – Mc Reynold method
Culture of lymphocytes with a medium of green coconut juice Nur Anisah Nur Anisah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 04 (1995)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

The study was aimed at investigating whether there were any difference on lymphocyte growth in in vitro by using RPMI 1640 and green coconut juice as medium. Lymphocytes were collected from spleen and bone marrow of 8 weeks old albino rats (Rattus norvegicus). Rats were sacrificed, subsequently spleen and bone marrow were taken and prepared for single cell suspension. Cells were counted using vital dye (Tripan biru) at 5x106 cells/ml. Twenty petri dishes were used. Half of them were used for spleen and the rest for bone marrow cell culture. In every group of those cells, petri were divided into two groups. One group used RPMI 1640 and the other a green coconut juice. Every petri dish was filled with 2 ml of suspension in cell media. All of the petri dishes were incubated in the incubator at 37°C, 5% CO2 for seven days. After 48 hours and 72 hours culture cells were examined and the percentage of viable cells was counted. Analysis of the viable cells between lymphocyte culture and bone marrow cells using RPMI .1640 and a green coconut juice was compared using Student's t-test and Anava. The results showed the increase of proliferation of viable cells from lymphocyte culture and bone marrow culture in both media hours were observed an after 72. As conclusion there is no significant difference between the growth of lymphocyte and bone marrow using RPMI 1640 and a green coconut juice (p>0,05).Key words: lymphocytes - cell culture - coconut juice - spleen cells - bone marrow cells

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