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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Core Subject : Science,
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Articles 2,170 Documents
The glucose uptake velocity and ability of target tissue cells in the elderly men (in vivo) Wasilah Rochmah, Wasilah Rochmah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 03 (2002)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Age is one of the impaired glucose tolerance risks. Impaired glucose tolerance may be caused by the decrease of pancreatic B cells insulin secretion, or by the decrease of target tissue cell glucose uptake. Glucose tolerance tests which were conducted in the elderly, showed that plasma insulin concentrations at 120th minute were still higher than at minute-0 (at fasting time), while blood glucose levels were still high. It generated an impression that insulin inefficiency exist in the elderly.Objective: To reveal that the cause of insulin inefficiency in the elderly is due to the decrease of target tissue cell glucose uptake velocity and/or in decrease uptake ability.Methods: Elderly men subjects and young men comparable controls were given 130mU/kg L8M/hour insulin and 20g% dextrose infusion, while blood glucose level have to be maintained in euglycemic situations (euglycemic clamp test). In 30 minutes with stable blood glucose level, the amount of 20% dextrose infusion were calculated as glucose uptake in 30 minute (euglycemic clamp time). From this results the glucose uptake velocity and ability (glucose uptake ability = ratio between glucose uptake velocity and plasma insulin concentration = insulin sensitivity index) between the elderly and the young men were compaired by t-test.Results: Euglycemic clamp tests were conducted to 4 elderly men of 65-74 years old, and 4 young men of 21-30 years old as controls. The results showed that the velocity of glucose uptake by the target tissue cells in those elderly men were significantly (p<0.05) lower (10.08 f 2.34 mg/kg LBM/min.) than young men group (17.78 ± 5.49 mg/kg LBMlmin.). One elderly men showed the lowest difference (0) of insulin sensitivity index compared to one control subject. In the remaining three (75%) subjects the average of insulin sensitivity index (0.11 f 0.03) showed a significant difference (p<0.05) compared to young men group 10.19 f 0.04).Conclusions: The result of this study indicated that the cause of insulin inefficiency in the elderly men was due to the decrease of glucose uptake velocity and ability.Key words: impaired glucose tolerance - pancreas B cells insulin secretion - velocity of glucose uptake ability of glucose uptake - euglycemic clamp technique.
Sensitivity and specificity of patch test in nickle allergic contact dermatitis I Ketut Darya Artana, I Ketut Darya Artana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 29, No 01 (1997)
Publisher : Universitas Gadjah Mada

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Abstract

Objectives of the study are as follows: first to compare the sensitivity and specificity of 5.0% nickle sulfate (NS) prepared by Pharmacy Unit Dr. Sardjito General Hospital and 5% NS International standard, and the second is to determine the ideal concentration of NS that can produce equal sensitivity and specificity with 5% NS International standard. Total number of sample is 86 subjects that have been recruited from Dermatology Clinic Sardjito General Hospital. Forty one patients with nickle allergic contact dermatitis and 45 patients with other dermatitis or healthy volunteers participated in the study. Every subject was tested with 5% NS International standard and 5.5%, 5.0%, 4.5%, 4.0%, 3.5%, 3.0%, 2.5%, 2.0% NS made by Pharmacy Unit Dr. Sardjito General Hospital. Result of the study shows that the sensitivity and specificity of 5% NS International standard are 95.12% and 95.56% respectively, while 5.0% NS made by Pharmacy Unit Sardjito General Hospital are 92.68% and 100% respectively (this differences is not statistically significant). Among different concentration of nickle sulfate made by Pharmacy Unit, 2.5% NS is the optimal concentration that can produce sensitivity and specificity which are equal to 5% NS International standard, both on the first and the second assessments (p = 0,06). Conclusion: 2.5% NS patch test made by Pharmacy Unit, Dr. Sardjito General Hospital can be used as standard material patch test for allergic contact dermatitis caused by nickel.Key word: nickle sulfate - pacth test - nickle allergic contact dermatitis.
The relationship between p53 expression, cell proliferation index and epidermal thickness in psoriasis Sekar Djatiningrum, Sekar Djatiningrum
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 33, No 03 (2001)
Publisher : Universitas Gadjah Mada

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Abstract

Background: The specific process among psoriatic lesions is keratinocyte hyperproliferation state, and one of the genes that controls cell cycle is wild type of p53 gene. The correlation between p53 overexpression and psoriatic keratinocytes hyperproliferation has not been established yet. Objective: To clarify the relationship between p53 overexpression and psoriatic keratinocyte proliferation index, as well as the epidermal thickness resulted from this proliferation.Methods: 50 paraffin-embedded tissue sections were chosen from block collection of Pathologic Anatomy Department. The p53 overexpression was measured by counting the number of positive cell among 500 cell stained by antibody anti p53. The cell proliferation index was measured by counting the number of black dot among 100 epidermal cells stained by silver nitrate. The epidermal thickness, represented by the thickness of epidermal rete ridges was measured by using calibrated micrometer in hematoxillin stained section.Results: p53 expression was significantly correlated in fairly degree with cell proliferation index (r, = + 0,47; P < 0,001) and significantly correlated in moderately degree with epidermal thickness (r, = + 0,57; P < 0,001). Cell proliferation index was significantly correlated in fairly degree with epidermal thickness (r1 = + 0,37; P < 0,004).Keywords: psoriasis - p53 - cell proliferation index - Ag NOR - epidermal thickness
Effectivity of pharmaca therapy compared to surgery In open-angle glaucoma Budihardjo, Budihardjo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 02 (1999)
Publisher : Universitas Gadjah Mada

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Abstract

Two problems arising in determining an appropriate glaucoma therapy are when to treat and how to treat. Open-angle glaucoma is treated when damage to the optic nerve has been demonstrated in the form of progressive pathologic cupping and or characteristic visual field defects, or when pressure is elevated to an extent that is likely to cause damage to the optic nerve. On the other hand, the interrelationship between medical and surgical therapy (argon laser trabeculoplasty & drainage surgery) is complex. Initial treatment of primary open-angle glaucoma has commonly been medical, with surgery undertaken only if medical treatment fails or is not well tolerated. However, this assumption is currently under study; in some cases initial surgery may prove to be more beneficial. Hence, review article was made to prove how beneficial medical or surgical treatment is.Key words: Primary open-angle glaucoma - medical therapy - argon laser trabeculoplasty - drainage surgery
The combined treatment of vitamin A eye drops, oral vitamin A , and oral doxycycline in meibomian gland dysfunction. Suhardjo, Suhardjo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 04 (2002)
Publisher : Universitas Gadjah Mada

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Abstract

Background: The frequency of meibomian gland dysfunction (MGD) patients is increasing in elderly and especially in female. Meanwhile the relationship of dry eye with meibomian gland dysfunction is close. Dry condition of eye will result in epitheliopathy, susceptibility to superinfection, scarring sequelae, and neovascularization in ocular surface. Vitamin A can prevent epitheliopathy and disturbance of tear film stability. Oral doxycycline inhibits lipase activity of any attendant microorganism.Objective: To evaluate the effect of systemic combined therapy vitamin A and doxycycline with vitamin A eye drops versus vitamin A eye drop and oral doxycycline in the management of meibomian gland dysfunction.Methods: The design of study was double blind randomized clinical trial. Setting was at Dr Sardjito Eye Clinics. The subjects were 60 MGD patients aged 50-79 years old, male and female were enrolled in this study and divided into two groups. The group I received vitamin A eye drops and oral doxycycline of 100 mg for 4 weeks. The group II was treated with oral vitamin Aof 6000 IU two times per day for 3 weeks, vitamin A eye drops, and oral doxycycline of 100 mg for 12th weeks. Both groups were followed at 4, 8, and 12 weeks for clinical signs, tear production (Schirmer test), quality of mucine (ferning test), and quality of ocular surface (rose bengal test).Results: Improvement was obtained in both group, in clinical signs of MGD as well as in tear production, quality of mucine, and ocular surface condition. There was no significant difference between both groups (p=0.58, RR =0.86, 95%, CI 0.21-3.50). The combined treatment of oral vitamin A, vitamin A eye drops and oral doxycycline of 100 mg in 12 weeks results in similar improvement with treatment by vitamin A eye drops and oral doxycycline in 4 weeks in MGD patients. The side effect of doxycycline was gastrointestinal problem in 2 patients.Conclusion: The result of this study supports that four weeks treatment is sufficient in achieving improvement of meibomian gland dysfunction patients.Key words: Vitamin A - doxycycline - meibomian gland dysfunction - four weeks
The effect of combined timolol maleat and p/locarpine acetazolamide on the decrease of intraocular pressure in the treatment of primary glaucoma Sutrisno, Sutrisno
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 29, No 03 (1997)
Publisher : Universitas Gadjah Mada

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Abstract

The study was aimed to compare the effects of combination of timolol maleat 0.5% and pilocarpine 2% to acetazolamide 250mg in the treatment of a primary glaucoma. We studied 13 patients with open angle glaucomas and 11 patients with closed angle glaucomas. This study was carried out in a randomized double blind clinical trial. The patients were divided into two groups, A and B. Group A consisted of patients treated with 250 mg acetazolamide t.i.d. and the group B was treated with combination of 0.5% timolol maleat b.i.d. and 2 % pilocarpine q.i.d. This one-day treatment was stopped and seven days later the treatment was interchanged between the two groups. The intraocular pressures were measured just before and two hours after the treatment. The mean decrease of 10P in group A was 17.042 ± 14.212mmHg and that of group B was 17.873 ± 9.005 mmHg. There was statistically no significant difference (p=0.804) between two groups. The mean decrease of 10P in primary open angle glaucoma in group A was 8.685 ± 6.389 mmHg and that of group B was 15.054 ± 5.994 mmHg. Statistically there was significant difference (p = 0.014). The mean decrease of 10P In primary closed angle glaucoma in group A was 26.918 ± 14.748mmHg and that of group B was 21.205 ± 10.993 mmHg. Statistically there was no significant difference (p = 0.3162). In conclusion, there was no significant difference between the use of acetazolamide 250mg q.i.d. the combination of timolol maleat 0.5% b.i.d. and pilocarpine 2% q.i.d. in reducing the 10P of primary glaucoma.Key words: primary glaucoma - lop decrease - acetazolamide - timolol maleat - pilocarpine.
Predicting time of death by postmortem erythrocyte osmotic fragility test in Sprague-Dawley white mouse Beta Ahlam Gizela, Beta Ahlam Gizela
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 33, No 02 (2001)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Death cases caused by crime require estimation of the time of death as a guide for searching who is the murderer. The common method has been used is by detecting hypostasis, rigidity, temperature decreasing, and decomposition. These methods are less accurate, since they are influenced by various factors. A new more accurate method is, therefore, required.Objectives: To establish a new method in predicting time of death by searching for relations between erythrocyte osmotic fragility and time of death.Methods: This research used Quasi Experiment Design. The subjects were 31 white male of mice 2 months old. The mouse blood was taken in a periodic time: ante mortem, 0, 1, 2, and 3 hours postmortem, and their erythrocyte osmotic fragility was detected by Modification Method of Osmotic Fragility Test. The reagent used was Saline Buffer Phosphate in gradual concentration, 0.2%, 0.3%, 0.4%, 0.5%, and 0.6%.Results: Data taken from this research were analyzed by regression analysis and ROC curve analysis.There was a significant positive correlation between time of death and erythrocyte osmotic fragility (r2 = 0.536, p< 0.01). Erythrocyte osmotic fragility of 0.5% (under curve area=0.826, p<0.01) was a cut off point at 1.5  hours post mortem (MSS=154.4%).Conclusion: Post mortem erythrocyte osmotic fragility test have a value in predicting time of death.Erythrocyte osmotic fragility test of 0.5% occured at      1.5  hours post mortem.Keywords: time of death - post mortem - erythrocyte osmotic fragility
Zingiber officinale volatile oil: clinical trial on Brugia Malayi infection in Kalimantan area Mulyaningsih, Mulyaningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 03 (1999)
Publisher : Universitas Gadjah Mada

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Abstract

Background: According to the fact, there is chance that the Zingiber officinale volatile oil can be developed for treatment elephantiasis in man. However, up to now there was no study yet on the efficacy of the phytopharmaca against filaria.Objective: The clinical trial was designed to know the effect and the safety of application of Zingiber officinale volatile oil as anti-filariasis agent in the treatment of Brugia malayi infection in Kalimantan area.Methods: In this study, 40 patients suffered from filariasis malayi were divided into 2 groups. Zingiber officinale volatile oil in honey syrup was given to each patient of group I. Honey syrup as plasebo was given to each patient of group II. The treatment was given for 28 consecutive days each. The density of microfilariae before and after treatment of each group were recorded and assessed using Pre-test and Post-test by using probit analysis.Results: This study showed that the average value of microfilaria density before and after treatment count per 60 ul blood in placebo group were 64.9 ± 96.4 and 90.4 ± 122.0. While in volatile oils group were, 92.1 ± 118.3 and 56.6 ± 77.0 respectively. MfD50 of placebo group before and after treatment is 27.75, and 44.99. MfDso of volatile oils group before and after treatment is 44.42, and 37.06.Conclusion: Zingiber officinale volatile oil can be developed for treatment elephantiasis in man.Key words : microfilaria density - phytopharmaca - volatile oil - filariasis malayi - elephantiasis.
The effect of megadosis of iron consumption on the absorption of zinc and copper in rat (Rattus rattus) determined in situ Sukarti Moeljopawiro, Sukarti Moeljopawiro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 30, No 03 (1998)
Publisher : Universitas Gadjah Mada

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Abstract

Oral administration of iron pill must be at an accurate dosis because high consumption of iron could impair the absorption of other minerals especially bivalent metals. Therefore, the objective of this study was to evaluate the effect of megadosis consumption of iron on the absorption of zinc and copper. The inorganic minerals were used in this study. The amount of mineral absorption was determined in situ. Forty male weanling rats were used in, this study. Rats were divided into 8 groups of 5, two groups for each treatment. Four ratios of Fe, Zn and Cu were used in this study (Fe : Zn : Cu = 5 : 1 : 1; 10 : 1 : 1; 20 : 1 : 1 and 50 ; 1 : 1). The amount of mineral absorption was determined using in vivo intestinal perfusion, and two perfusion rates were used 0.5 ml/minute and 0.33 ml/minute. It was found that high iron consumption (50 : 1 : 1) could impair the zinc absorption at perfusion rate of 0.33 ml/minute and no zinc absorption occur at perfusion rate of 0.5 ml/minute. However, this high iron consumption did not influence the copper absorption both at perfusion rate of 0.5 ml/minute and 0.33 ml/minute. It can be concluded that high consumption of iron could impair the zinc absorption but not copper absorption.Key words : iron - zinc - copper - mineral absorption - in situ perfusion
Phototoxicity inhibition effect of vitamin c and glutathione against several photosensitizers Rosmelia, Rosmelia
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 35, No 3 (2003)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Ultraviolet B radiation on the skin, in the presence of photosensitizers such as quinolones, sulfacetamide, and non-steroid antiinflammatory.could induce phototoxicity due to cell membrane photo-oxidation. Antioxidants, such as vitamin C and glutathione, have been reported capable to neutralize free radicals and prevent oxidative reactions. Scientific report about the role of such antioxidants in the prevention of phototoxicity due to photosensitizers on above, is still limited.Objectives: To compare phototoxicity inhibition effect between vitamin C and glutathione and phototoxicity potency of several photosensitizersMaterials and Methods: A simple experimental design is used to compare phototoxicity potency of 0.08 mg/ml ofloxacin, 0.004 mg/ml furosemide, and 0.02 mg/ml ketoprofen, and effects of 0.2 mg/ml vitamin C, and 0.12 mg/ml glutathione on phototoxicity reactions of erythrocytes exposed to 1.6 J/cm2 UVB. Phototoxicity reaction was measured by hemoglobin released by targeted erythrocytes using spectrophotometer.Results: Hemoglobin released by photosensitizers were ofloxacin 6.760±.1.669, furosemide 6.532±1.271, ketoprofen 6.710 ±1.826 g/dI respectively. There was no significant difference of haennoglobine released among them (p>0.051. Addition of vitamin C could reduce phototoxicity of photosensitizer very significantly (p

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