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Laboratory blood group examination of proteolysis degradation human blood Beta Ahlam Gizela, Beta Ahlam Gizela
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 37, No 01 (2005)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.014 KB)

Background: Blood group examination has many purposes and one of them is identification. In several forensic cases there is incompatibility of blood group in corpse and in other evidences usually used blood group examination is serum agglutination method. From the previous study, it was found that there was increasing osmotic fragility of red cell. For that reason, we need to know how the result of blood group tests in degradation human blood.Objective: The purpose of this study is to know blood groups of proteolysis degradation human blood.Method: This study was an experimental study. The subjects was people that have blood group A, B, AB, and 0. Blood samples were examined serially for blood grouping, when the samples were just taken, after stored in room temperature, with addition of protease enzyme (tripsin) in 20 seconds, 1 hour, 2 hours, 3 hours, and 24 hours, and without protease enzyme after 7 days. The data was analysed using chi-square statistics.Result: This study showed there was significant proportionally different(p<0.05) in blood group chang-ing of non-0 blood group to be 0 blood group after stored the blood in 3 hours with protease enzymeÂ addition (5(16.68%)). In 24 hours, it was showed that all of non-0 blood group changed to 0 blood groupÂ (100%). Blood group examination of the blood without protease enzyme addition stored for 7 daysÂ showed that all of them were observed as 0 blood group.Conclusion: There is blood group changing by agglutination method from non-O blood group to 0 blood group in proteolysis degradation blood.Â Key words: blood group - proteolysis degradation - group changing

Study of medical report on justice examination result at Dr. Sardjito Hospital Beta Ahlam Gizela, Beta Ahlam Gizela
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 2 (2004)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.613 KB)

Background: Every human violation or crime case, in which there is human as a victim needs medical proofing, namely the Medical Report for justice or Visum et Repertum in Indonesia. We also have an obligation in keeping medical confidentiality in medical practice. It is therefore, necessary to study relevance regulation of examination and disclosing medical confidentiality for justice.Objectives: The purpose of this study is to analyse management of Visum et Repertum of life person at Sardjito Hospital Yogyakarta.Methods: Visum et Repertum of life person at Medical Record Department of Sardjito Hospital was reviewed. The MEG protocol for examination and reporting from International Amnesty used as a standard. Results: The study shows that management of Visum et Repertum of life person at Sardjito Hospital was administratively inappropiate. There were some discrepancies between regulation and practice. There is no agreement between each doctors in managing cases. Generally, Visum et Repertum was accepted in content, but for the future an improvement is needed as International Amnesty.Conclusions: Management of life person cases for Visum et Repertum at Sardjito Hospital do not international standard.Key words: visum et repertum - living person - medical confidentiality - informed consent

Predicting time of death by postmortem erythrocyte osmotic fragility test in Sprague-Dawley white mouse Beta Ahlam Gizela, Beta Ahlam Gizela
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 33, No 02 (2001)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.901 KB)

Background: Death cases caused by crime require estimation of the time of death as a guide for searching who is the murderer. The common method has been used is by detecting hypostasis, rigidity, temperature decreasing, and decomposition. These methods are less accurate, since they are influenced by various factors. A new more accurate method is, therefore, required.Objectives: To establish a new method in predicting time of death by searching for relations between erythrocyte osmotic fragility and time of death.Methods: This research used Quasi Experiment Design. The subjects were 31 white male of mice 2Â months old. The mouse blood was taken in a periodic time: ante mortem, 0, 1, 2, and 3 hours postmortem, and their erythrocyte osmotic fragility was detected by Modification Method of Osmotic FragilityÂ Test. The reagent used was Saline Buffer Phosphate in gradual concentration, 0.2%, 0.3%, 0.4%,Â 0.5%, and 0.6%.Results: Data taken from this research were analyzed by regression analysis and ROC curve analysis.There was a significant positive correlation between time of death and erythrocyte osmotic fragility (r2 =Â 0.536, p< 0.01). Erythrocyte osmotic fragility of 0.5% (under curve area=0.826, p<0.01) was a cutÂ off point at 1.5Â hours post mortem (MSS=154.4%).Conclusion: Post mortem erythrocyte osmotic fragility test have a value in predicting time of death.Erythrocyte osmotic fragility test of 0.5% occured atÂ Â Â Â Â 1.5Â hours post mortem.Keywords: time of death - post mortem - erythrocyte osmotic fragility

Postmortem hemoglobin concentration changing in Sprague-Dawley white mouse Beta Ahlam Gizela, Beta Ahlam Gizela
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 33, No 04 (2001)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.538 KB)

Background: Postmortem changes in a death body have a lot of purposes, one of them is predicting the time of death. The common method used to predict the time of death is by detecting hypostasis, rigidity, decreasing temperature, and decomposition.Objectives: To find out postmortem hemoglobin concentration changing pattern.Methods: This research is a preliminary study. We used Quasi Experimental Design. The subjects were 31 white male mice aged of two months old. The mouse blood was taken in a periodic time: antemortem, 0, 1, 2, and 3 hours postmortem, and hemoglobin concentration was examined using Sahli method.Results: Data taken from this research were analyzed by regression analysis and t-test. The result showed that postmortem hemoglobin against time pattern was a curve. The hemoglobin concentration is significantly decreased in the first hour (p<0.05), and then increased 2 hours later (p>0.05). There was no significant difference between antemortem and 0 hour postmortem (p>0.05). There was a significant difference between antemortem and 1, 2, and 3 hours postmortem (p<0.05).Conclusion: Postmortem hemoglobin concentration changing pattern is a curve, not linear. The hemoglo-bin concentration decreased in the first hour since death (statistically significant), then, increased in the second and third hour postmortem (statistically not significant). There is a significant difference between antemortem and 1, 2, and 3 hours postmortem.Keywords: Hemoglobin concentration -postmortem changes - time of death -blood

Using Sleman’s Verbal Autopsy Health and Demographic Surveillance Data to Distinguish Ways of Death Beta Ahlam Gizela; Idha Arfianti Wiraagni; Suhartini; Nurholis Majid Public
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15713

Death can occur anywhere, with or without prior treatment by a healthcare professional. A good and complete death registry will meet the information needs. Such a registry will also enhance community safety efforts and policymaking in the health and population sectors. Analysis of risk factors, signs, and symptoms observed before death provides useful information for estimating diseases or causes of death. Verbal autopsy data must be explored in more depth to protect public law and improve health statistics.Methods: This research used observational design. Data obtained from the Health and Demographic Surveillance System of Sleman. Research subjects were all deaths recorded in verbal autopsy document from 2014-2018. Descriptive statistical analysis was used.Results: Differences in the proportion of gender from 2014-2017 were observed, where the number of women was higher than men. Most deaths were above 65 years of age. Most deaths were natural deaths. Unnatural deaths were caused by traffic accidents, drowning, murder, and others, with the most number of cases occurring in 2014 (12%). Indeterminate deaths occurred annually, with the most cases in 2017 (13%). Conclusions: Verbal autopsy has succeeded in distinguishing between natural and unnatural death.

Laboratory blood group examination of proteolysis degradation human blood Beta Ahlam Gizela Beta Ahlam Gizela
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 37, No 01 (2005)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.014 KB)

Background: Blood group examination has many purposes and one of them is identification. In several forensic cases there is incompatibility of blood group in corpse and in other evidences usually used blood group examination is serum agglutination method. From the previous study, it was found that there was increasing osmotic fragility of red cell. For that reason, we need to know how the result of blood group tests in degradation human blood.Objective: The purpose of this study is to know blood groups of proteolysis degradation human blood.Method: This study was an experimental study. The subjects was people that have blood group A, B, AB, and 0. Blood samples were examined serially for blood grouping, when the samples were just taken, after stored in room temperature, with addition of protease enzyme (tripsin) in 20 seconds, 1 hour, 2 hours, 3 hours, and 24 hours, and without protease enzyme after 7 days. The data was analysed using chi-square statistics.Result: This study showed there was significant proportionally different(p<0.05) in blood group chang-ing of non-0 blood group to be 0 blood group after stored the blood in 3 hours with protease enzyme addition (5(16.68%)). In 24 hours, it was showed that all of non-0 blood group changed to 0 blood group (100%). Blood group examination of the blood without protease enzyme addition stored for 7 days showed that all of them were observed as 0 blood group.Conclusion: There is blood group changing by agglutination method from non-O blood group to 0 blood group in proteolysis degradation blood. Key words: blood group - proteolysis degradation - group changing

Study of medical report on justice examination result at Dr. Sardjito Hospital Beta Ahlam Gizela Beta Ahlam Gizela
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 2 (2004)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.613 KB)

Background: Every human violation or crime case, in which there is human as a victim needs medical proofing, namely the Medical Report for justice or Visum et Repertum in Indonesia. We also have an obligation in keeping medical confidentiality in medical practice. It is therefore, necessary to study relevance regulation of examination and disclosing medical confidentiality for justice.Objectives: The purpose of this study is to analyse management of Visum et Repertum of life person at Sardjito Hospital Yogyakarta.Methods: Visum et Repertum of life person at Medical Record Department of Sardjito Hospital was reviewed. The MEG protocol for examination and reporting from International Amnesty used as a standard. Results: The study shows that management of Visum et Repertum of life person at Sardjito Hospital was administratively inappropiate. There were some discrepancies between regulation and practice. There is no agreement between each doctors in managing cases. Generally, Visum et Repertum was accepted in content, but for the future an improvement is needed as International Amnesty.Conclusions: Management of life person cases for Visum et Repertum at Sardjito Hospital do not international standard.Key words: visum et repertum - living person - medical confidentiality - informed consent

Predicting time of death by postmortem erythrocyte osmotic fragility test in Sprague-Dawley white mouse Beta Ahlam Gizela Beta Ahlam Gizela
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 33, No 02 (2001)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.901 KB)

Background: Death cases caused by crime require estimation of the time of death as a guide for searching who is the murderer. The common method has been used is by detecting hypostasis, rigidity, temperature decreasing, and decomposition. These methods are less accurate, since they are influenced by various factors. A new more accurate method is, therefore, required.Objectives: To establish a new method in predicting time of death by searching for relations between erythrocyte osmotic fragility and time of death.Methods: This research used Quasi Experiment Design. The subjects were 31 white male of mice 2 months old. The mouse blood was taken in a periodic time: ante mortem, 0, 1, 2, and 3 hours postmortem, and their erythrocyte osmotic fragility was detected by Modification Method of Osmotic Fragility Test. The reagent used was Saline Buffer Phosphate in gradual concentration, 0.2%, 0.3%, 0.4%, 0.5%, and 0.6%.Results: Data taken from this research were analyzed by regression analysis and ROC curve analysis.There was a significant positive correlation between time of death and erythrocyte osmotic fragility (r2 = 0.536, p< 0.01). Erythrocyte osmotic fragility of 0.5% (under curve area=0.826, p<0.01) was a cut off point at 1.5 hours post mortem (MSS=154.4%).Conclusion: Post mortem erythrocyte osmotic fragility test have a value in predicting time of death.Erythrocyte osmotic fragility test of 0.5% occured at 1.5 hours post mortem.Keywords: time of death - post mortem - erythrocyte osmotic fragility

Postmortem hemoglobin concentration changing in Sprague-Dawley white mouse Beta Ahlam Gizela Beta Ahlam Gizela
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 33, No 04 (2001)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.538 KB)

Background: Postmortem changes in a death body have a lot of purposes, one of them is predicting the time of death. The common method used to predict the time of death is by detecting hypostasis, rigidity, decreasing temperature, and decomposition.Objectives: To find out postmortem hemoglobin concentration changing pattern.Methods: This research is a preliminary study. We used Quasi Experimental Design. The subjects were 31 white male mice aged of two months old. The mouse blood was taken in a periodic time: antemortem, 0, 1, 2, and 3 hours postmortem, and hemoglobin concentration was examined using Sahli method.Results: Data taken from this research were analyzed by regression analysis and t-test. The result showed that postmortem hemoglobin against time pattern was a curve. The hemoglobin concentration is significantly decreased in the first hour (p<0.05), and then increased 2 hours later (p>0.05). There was no significant difference between antemortem and 0 hour postmortem (p>0.05). There was a significant difference between antemortem and 1, 2, and 3 hours postmortem (p<0.05).Conclusion: Postmortem hemoglobin concentration changing pattern is a curve, not linear. The hemoglo-bin concentration decreased in the first hour since death (statistically significant), then, increased in the second and third hour postmortem (statistically not significant). There is a significant difference between antemortem and 1, 2, and 3 hours postmortem.Keywords: Hemoglobin concentration -postmortem changes - time of death -blood

Pengurusan Jenazah Covid-19: Refleksi Kritis Sebagai Simbol Dakwah Modern, Kesehatan, dan Humanisme Beta Ahlam Gizela; Ahnav Bil Auvaq; Arwani Munib
Islamic Management and Empowerment Journal Vol 3, No 1 (2021): Islamic Management and Empowerment Journal
Publisher : IAIN Salatiga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18326/imej.v3i1.19-36

Pengurusan jenazah memiliki dimensi agama (dakwah) dan medis, terutama dalam konteks pencegahan penyebaran Covid-19.. Tujuan penelitian ini adalah memahami lebih mendalam tentang permasalahan pengaturan prosedur pengurusan jenazah Covid-19, meliputi prosedur pengurusan jenazah dari perspektif fikih Islam dan medis. Pendekatan penelitian yang digunakan adalah studi pustaka. Jenis penelitian ini adalah penelitian kualitatif dengan menggunakan analisis konsep dan komparasi untuk memecahkan masalah. Hasilnya adalah pengurusan jenazah Covid-19 berdasar panduan medis dan fikih Islam memiliki kesesuaian, sehingga kebutuhan masing-masing dapat dipenuhi. Kemudian dalam perspektif humaniora, seperti konflik ideologis dan sosial budaya dapat diberikan alternatif melalui metode dakwah dengan media digital.

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