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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Published by Universitas Gadjah Mada
ISSN : 01261312     EISSN : 23563931     DOI : http://dx.doi.org/10.19106/JMedSci005303202101
Core Subject : Science,
Immunology & microbiology Neuroscience
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 2,170 Documents
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Effect of swimming and asthmatic exercise on forced expiratory volume in 1 second (FEV1) and levels of cortisol hormone in asthmatics patients Achmad Djunaedi, Rahmaya Nova Handayani, Denny Agustiningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Bronchial asthma is one of the allergic diseases characterized by reversible bronchial narrowing dueto bronchial hyperactivity and obstruction of respiratory tract. The prevalence and hospitalizationrates of bronchial asthma keep increasing from year to year, causing asthma to be the top ten causesof morbidity and mortality in Indonesia. The decrease of the forced expiratory volume in 1 second(FEV1) is one of the indicators of the respiratory tract obstruction. Moreover, the cortisol hormonelevel is also associated with asthma. Asthma can be treated not only with pharmacological interventionbut also physical exercises. The aim of the study was to assess the increase of FEV1 and cortisolhormone level after swimming and asthmatic exercise on asthmatic patients. This was a quasiexperimental study using pre and post test control group design. Two groups of 10 asthmaticpatients each conducted swimming and asthmatic exercise as intervention. On pre, middle and postswimming or asthmatic exercise, those two groups underwent the measurement of FEV1 using aspirometer and cortisol hormone levels using ELISA methods. The result showed that the value ofFEV1 and cortisol hormone levels increased significantly after swimming and asthmatic exercise(p<0.05). Moreover, the improvement of FEV1 value and cortisol hormone levels of asthmaticpatients in swimming were higher than asthmatic exercise. In conclusion, swimming and asthmaticexercise can increase the value of FEV1 and the levels of the cortisol hormone.Keywords : swimming - asthmatic exercise - FEV1 - cortisol hormone - asthma
he Role of red blood cell indices to identify iron deficiency in anemic pulmonary tuberculosis patient Tri Ratnaningsih, Tri Ratnaningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 01 (2008)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: iron deficiency anemia (IDA) is often associated with infectionlinflammation disease, such as pulmonary tuberculosis. The biochemical parameters tests of iron metabolism are expensive relatively and not always available in some health centers.Objective: The aim of this study was to know the role of red blood cell indices to identify iron deficiency on pulmonary tuberculosis patient with anemia using a sTfR-based biochemical classification of ACD and ACD with iron deficiency (COMB!).Methods: The patient population consisted of 68 consecutive anemic pulmonary tuberculosis patients at the Sardjito Hospital and Balai Pengobatan Penyakit Paru-Paru 0.1. Yogyakarta, Indonesia.Result: Of 68 subjects, 53 had anemia of chronic disease (ACD) and 15 had iron deficiency anemia (COMBI). It was founded a significant difference between ACD group and COMBI group. There were significant correlations between red blood cell indices and sTfR. The most valid parameter to diagnose 10 is MCHC < 31.55 g/dL (Sn= 67%, Sp= 75%), followed by MCV < 81.65 fL (Sn= 62%, Sp= 73%), MCH < 25.75 pg (Sn= 67%, Sp= 70%), and lastly ROW> 14.95 (Sn= 73%, 57%). The diagnostic performances of this parameter at levels commonly used: MCV < 76 fL (Sn= 13%, Sp= 85%), MCH < 27 pg (Sn= 100%, Sp= 45%), MCHC < 31g/dL (Sn= 13%, Sp= 85%), and RDW > 15 (Sn= 67%, 60%).Conclusion: The usage of red blood cell indices can help us to identify iron deficiency in anemic pulmonary tuberculosis patients. For practical reason, more convenience if we use the value that is widely accepted. However, the red blood cell indices cannot be used solely, but must be combined for a greater accuracy.Keywords: red blood cell indices - soluble transferrin receptor - iron deficiency - anemia - pulmonary tuberculo
Activity of -terpineol as a potential anticancer candidate: cytotoxicity, proapoptotic and antiproliferative evaluation in TD47 cell lines Mustofa, Puguh Indrasetiawan Indwiani Astuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Previous study has shown that -terpineol has a cytotoxic effect on NCI-H69 cancer cell line.This study aimed to evaluate cytotoxicity, proapoptotic, and antiproliferative activities of -terpineol in T47D cell lines. The cytotoxic activity of -terpineol was determined using MTT cellviability assay, while apoptosis was tested by the acridine orange-ethidium bromide stainingmethod. The cell cycle was evaluated by flowcytometry method. The results showed that -terpineol had cytotoxic effect on the T47D cells with the IC50 value of 135.00 ± 8.74 M.Furthermore, -terpineol induced the cells apoptosis in a dose-dependent manner. Flowcytometryanalysis showed that -terpineol induced the T47D cell distribution in Go/G1 phase, howeverinhibited cell distribution in S phase. This study suggests that -terpineol has a potential anticanceractivity.
Diagnostic test of mammography in a palpable mass of the breast Bambang Udji Djoko Rianto, Rosalia Sri Sulistijawati Henry Kusumo Husodoputro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 02 (2008)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Breast cancer is the most common cancer in Indonesia after cervical cancer. The diagnosis of breast cancer is based on triple diagnostics, clinical diagnostic, mammograph, and fine needle aspiration biopsy (FNAB). Mammograph is a non-invasive radiologic imaging to demonstrate microcalcification as an early sign of breast cancer.Objective: The aim of this study was to measure the validity of mammograph in a palpable mass of the breast.Methods: Study design for this study was a diagnostic test that compared mammograph with histopatology result as the gold standard. Study was performed between March 2006 and September 2007 in Sardjito General Hospital, Yogyakarta. There were 79 palpable masses of the breast that were included as the subjects of this study. They were mammographed and examined histopatologically. Descriptive analysis with McNemar non parametric test with 2x2 table was performed by a computer software program to calculate sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), positivelikelihood ratio (positive LR) and negative likelihood ratio (negative LR). .Results: Sensitivity, specificity, PPV, NPV, positive LR and negative LR of mammograph in diagnosing a breast palpable mass were 87.18%,87.50%,87.18%,87,50%,7, and 0.146, respectively Conclusion: Mammograph had a high validity in diagnosing breast palpable masses.Key words: breast palpable mass - mammograph - diagnostic test - validity.
Sexual dimorphism of pelvic girdle: pelvimetry and pelvic types in Javanese Etty Indriati, Etty Indriati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 01 (2007)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Each population has certain phenotypic traits that are unique compared to other populations. Pelviss size and morphology are two of them. A western formula of obstetric conjugate = diagonal conjugate - 1.5 to 2.0 em; may not be applicable to Javanese population; and sexual traits in Western pelves may not entirely be the same with Indonesians.Objective: to get a formula for obstetric conjugate, and conduct various pelvic measurements in order to find out the metric pattern as well as the distribution of pelvic types in Javanese.Material and Methods: 27 pelves consisting of 6 females and 21 males housed at the Department of Anatomy, Embryology and Anthropology were studied by connecting the sacrum with the right and left pelvis forming pelvic girdles. Superior inlet, diagonal conjugate, angle of greater sciatic notch, diameter and width of pelves were among the metric studied.Results: Widely used traits such as greater sciatic notch that are used in Western literature to differentiate male and female pelves in Caucasian is not consistent for Javanese pelves. The most consistent skeletal traits for sexing female pelvis are wide subpubic angle (> 75 degree), preauricular sulci, flat posterior pubic symphisis, and short ischiopubic rami. The common type of ginecoyd pelvis in Javanese (48%) is in accord with western pelvis reaching 50%. Compared to the western obstetric conjugate, Javanese obstetric conjugate has a slightly different formula = diagonal conjugate - 1 to 2.6 cm.Conclusions: Several traits in sexual dimorphic of pelvic bone in Javanese do not match with skeletal traits widely used in Western literature, portraying the importance of studying regional human biological variation. The obstetric conjugate of Javanese pelves is not entirely the same with Western formula, though the difference is not significant.Key words: Javanese, pelves, sexual dimorphism, obstetric conjugate
The different expressions of urokinasetype plasminogen activator, urokinasetype plasminogen activator receptor, and plasminogen activator inhibitor-1. Several scores of cell pleomorphism, number of mitosis and keratiniza Totok Utoro, Irianiwati, Ahmad Ghozali
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 03 (2008)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Urokinase plasminogen activator (uPA) system plays important role in cancer invasion and metastasis. The real function of PAI-1 is to protect extracellular matrix from degradation. But, there are still arguments about the function of PAI-1. In colorectal cancer, breast cancer, and liver cancer, high expressions of uPA and PAI-1 are related to poor prognosis of the patients. In squamous cell carcinoma of the cervix, the role of uPA system in its relation with histological grading (consists of several scores of cell pleomorphism, number of mitosis and keratinization index) are still unclear.Aim: To find out the different expressions of uPA, uPAR, PAI-, and ratio of uPA!PAI-1 among several scores of cell pleomorphism, number of mitosis and keratinization index of cervical cancerMethods: Thirty five paraffin blocks of squamous cell carcinoma of the cervix were grouped histologically based on the score of cell pleomorfism, number of mitosis, and keratinization index. The specimens were stained immunohistochemically with MoAb against uPA, uPAR, and PAI-1 with chromogen DAB and counterstain Meyer Hematoxyllin. The expression of uPA, uPAR, PAI-1, and ratio of uPA/PAI-1 were counted based on the number of positive expression tumor cells!100 tumor cells in five microscopic fields. The different expression of each variable will be analyzed with One Way Anova analysis.Result: There were statistically insignificant differences between the expressions of uPA, uPAR, PAI-1, and ratio of uPA/PAI- based on the score of cell pleomorfism (p = 0.463, 0.731, 0.541, 0.460, respectively), keratinization index (p = 0.207, 0.062, 0.231, 0.576, respectively), and nlimber of mitosis (p = 0.359, 0.125, 0.428, 0.340, respectively) of squamous cell carcinoma of the cervix.Conclusion: The result suggested that the expressions of uPA, uPAR, PAI-1, and ratio of uPA/PAI-1 did not have important roles in cells pleomorfism, number of mitosis, and keratinization index of cervical cancer.Key words: uPA - uPAR - PAI- - ratio of uPA!PAI-1 - cell pleomorphism - number of mitosis - keratinization index - cervical cancer
Comparasion of Sport Urine Protein Creatinine From Three Different Time Urine Samples in Children With Primary Nephrotic Pungky Ardani, Jusuf Hartanto Mohammad Juffrie
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Universitas Gadjah Mada

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Abstract

Background: First voiding of morning urine protein/creatinine ratio on a single voided urine sample correlates well with 24 hour urine collection and can be used to detect and monitor proteinuria in children. Objective: Aim of this study was to determine whether the random urine samples can be used as reliable indicator for spot urine protein/creatinine ratio.Methods: 40 primary nephrotic syndrome children aged 1-16 years admitted to the Department of Pediatric, Dr Sardjito hospital were included in this study. Urine samples from three different times were collected: first voiding of morning urine, noon voiding and night voiding. The three samples were analyzed for protein level, creatinine level and protein/creatinine ratio of spot urine. The protein/creatinine ratio was compared and correlation was calculated according to the three different time collections.Results: There was no significant difference of noon and night protein/creatinine ratio compared with first voiding of morning urine protein creatinine ratio (p> 0.05). The correlation of protein/creatinine ratio between the three samples was significantly high (r=0.95, 0.96 and 0.98, p < 0.01).Conclusions: For quantifying proteinuria, there is convincing evidence that the random urine samples can be used to predict the level of protein excretion as measured in a 24-hour sample.Keywords: Proteinuria, protein, creatinine ratio, primary nephrotic syndrome
Targeted phototherapy for skin diseases Arief Budiyanto, Sa’adatul Huriyah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Phototherapy is a therapeutic strategy in dermatology for treating several skin diseases.Conventional phototherapy has some disadvantages, hence a targeted phototherapy that emitsselective laser or ultraviolet (UV) light and targets specific area of affacted skin has been developed.Targeted phototherapy is considered to be more aggressive and has higher efficacy. Severaltargeted phototherapy devices emit one or several types of light. The use of targeted phototherapyhas been studied in vitiligo, psoriasis, eczema, keloid, hypopigmented scar or striae alba, andoral lichen planus.
Diabetes mellitus type 1 in congenital rubella syndrome: a case report E.S. Herini, Carina Lisa Madarina Julia
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 04 (2008)
Publisher : Universitas Gadjah Mada

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Abstract

Corina Lisa, Madarina Julia, E.S. Herini . Diabetes Mellitus Type 7 in Congenital Rubella SyndromeDiabetes mellitus type 1 has been reported as a very rare delayed manifestation of congenital rubella syndrome. We reported a confirmed case of congenital rubella syndrome that developed diabetes mellitus at the age of seven years. The girl had been complaining of diabetes symptoms since six months before admission, but had been misdiagnosed. The patient finally came with symptoms of diabetic ketoacidosis, a life threatening complication of diabetes mellitus. Diabetes mellitus type 1 as a delayed manifestation of congenital rubella syndrome has to be kept in mind to prevent missing the diagnosis.Key words: rubella infection - congenital rubella syndrome - diabetes mellitus type 1ABSTRAKCorina Lisa, Madarina Julia, E.S. Herini - Diabetes Mellitus Tipe 7 pada Sindroma Rubella KongenitalDiabetes melitus adalah salah satu manifestasi lambat sindrom rubella kongenital yang jarang, tetapi perlu diwaspadai. Kami melaporkan satu kasus sindrom rubela kongenital yang mengalami diabetes melitus tipe 1 pad a usia 7 tahun. Meskipun gejala diabetes telah dikeluhkan sejak enam bulan sebelum masuk rumah sakit, penyakit diabetes tetap tidak terdiagnosis. Penderita akhirnya datang dengan gejala dan tanda ketosidosis diabetikum, suatu komplikasi diabetes mellitus yang mengancam jiwa. Manifestasi lambat sindrom rubella yang berupa diabetes mellitus tipe I harus diwaspadai untuk mencegah tidak terdiagnosanya penyakit tersebut.
The droplet size changes of 1% propofol before and after the storage procedure for 6 and 24 hours periods Djayanti Sari, Basuki Rahmat Bambang Suryono S
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Propofol (2,6-diisopropylphenol) is a popular anesthetic agent for induction and anestheticmaintenance. Propofol preparation is formulated as a lipid macroemulsion that is thermodynamicallyunstable and degraded over time, causing the enlargement of droplet size. The degradation is fasterwhen propofol emulsion preparation package has been opened. This droplet enlargement results indecreasing propofol releasing-rate and its concentration. The risk of embolism occurs whenever themean droplet size (MDS) exceeds Food and Drug Administration (FDA) limit (<0.45) and fat globulepopulation percentage >5 m (PFAT5) is <0.05%. In the daily practice, some propofol emulsionpreparation are often used although they are already opened and saved more than 24 hours. The aimof this study was to evaluate the droplet size changes of propofol emulsion preparation duringstorage. This was an observational study using cohort prospective design. The droplet size changesof sixteen propofol preparation samples were evaluated before and after storage procedure for 6 and24 hours in the refrigerator at the temperature of 4ºC. The droplet diameter was determined under alight microscope. Mean droplet size before and after storage were calculated and analyzed by oneway analysis of variance (ANOVA), followed by Tukey’s post hoc test with 95% confidence interval(p<0.05). The droplet diameter of propofol emulsion preparation increased significantly (p<0.05)after storage procedure for 6 h (MDS value were: 247 ± 22 nm) and 24 h (278 ± 29 nm) comparedto before storage (225 ± 24 nm). The PFAT5 at each interval time was 0%. There was no color andhomogeneity changing at each interval time (n=16/100% each time interval). In conclusion, there isa change of propofol emulsion preparation droplet size after storage procedure for 6 to 24 hours attemperature of 4ºC compared to before storage.

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