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Dentika Dental Journal

dentika
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Published by Universitas Sumatera Utara

ISSN : 1693671X EISSN : 2615854X DOI : -

Core Subject : Health,

Dentistry

d e n t i k a DENTAL JOURNAL is one of the journals managed by TALENTA Universitas Sumatera Utara which first published in 2015. This is an online scientific journal that publishes articles and scientific work from Researches, Case Reports and Literature Reviews in Dentistry and Dental Science. The scopes are varied from Dental Surgery, Dental Forensics, Oral Biology, Oral Medicine, Dental Public Health and Preventive Dentistry, Paediatric Dentistry, Dental Materials and Technology, Conservative Dentistry, Orthodontics, Periodontics, Prosthodontics, to Dental Radiology.

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Articles 390 Documents

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EFFECT OF LATEX AND EXTRACT OF JATROPHA CURCA LINN ON EXPRESSION OF SUBSTANCE P (SP) AND CYCLOOXYGENASE-2 OF DENTAL PULP: EFEK LATEKS DAN EKSTRAK JATROPHA CURCA LINN TERHADAP EKSPRESI SUBSTANSI P DAN COX2 PULPA GIGI Irmaleny; Narlan Sumawinata; Dewi Fatma
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Inflammatory pulpal pain may arise due to the increased pressure inside the pulp or the release of prostaglandin E2(PGE2). In the inflamed pulp, levels of PGE2 and substance P (SP) is higher than those of normal pulp. PGE2 sensitizes all nociceptor while SP can induce the activity of cyclooxygenase-2 (COX- 2) and is an exitatory neurotransmitter. Jatropha curcas Linn latex is widely used for pulpal pain relief. The aim of this study was to evaluate the effect of the latex and extract of J. curcas on the dental pulp expression of COX-2 and SP. Thirty-six pulpitis-induced dental pulp of Macaca fascicularis , were divided into 3 groups: Group 1 served as controls, Group 2 was given latex, and Group 3 was given extracts. The ELISA assay was used to determine the levels of SP and COX-2. SP data was analyzed with ANOVA (p <0.05) while the COX-2 data was analyzed with Mann Whitney. The results showed that the levels of SP (pg/mL) of the control, latex, and extract group were 28.94; 26.22; 28.89 respectively, while levels of COX-2 (ng/ml) of control, latex, and extract group were 0.04; 0.08; 0.10 respectively. In conclusion , J. curcas can reduce the levels of SP, J. curcas latex has lower levels of SP than extract, but does not provide clear results in decreased levels of COX- 2. Further study requires the mechanism of SP, and the concentration of COX- 2 needs to be further investigated using different methods.

PENGARUH PEMBERIAN KOMBINASI SEL PUNCA PULPA GIGI DAN RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2 TERHADAP KADAR FOSFATASE ALKALI PADA PULPA GIGI TIKUS TERINFLAMASI: COMBINATION OF RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN- 2 AND DENTAL PULP STEM CELLS ENHANCED EXPESSION OF ALKALINE PHOSPHATASE ON INFLAMED RATâ€™S PULP Endang W Bachtiar; Mindya Yuniastuty; Aimee Monica; Boy M Bachtiar
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Pulp irritation will form reparative dentin as a defense mechanism. Currently, the materials used to help the defense pulp still have many shortcomings. Therefore, the effect of dental pulp stem cells (DPSC) and rhBMP- 2 combination on alkaline phosphatase (ALP) expresion in rat â€™s dental pulp was analyzed in this research. Lipopolysaccharide was used to irritate the pulp of 12 Sprague-Dawley rats. Materials were then applied to each group and ALP expression was performed on the first and second week. The result showed that in the first week, giving a combination of pulp stem cells with recombinant BMP-2 had not been visible to increase expression of alkaline phosphatase (ALP), a biomarker regeneration of pulp, but the increase in ALP expression occurred in the second week. In conclusion, a combination of pulp stem cells and rhBMP-2 can increase the expression of ALP on inflamed ratâ€™s pulp.

PENGALAMAN KARIES GIGI MOLAR PERTAMA PERMANEN BERDASARKAN RIWAYAT PENDIDIKAN TAMAN KANAK-KANAK: CARIES EXPERIENCE OF PERMANENT FIRST MOLAR TOOTH BASED ON THE PRE- ELEMENTARY EDUCATIONAL BACKGROUND Ristya Widi Endah Yani; Windhy Putri Utami
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Children in pre-educational ages are vulnerable to the caries of the permanent first molar. Early prevention on this problem may be conducted through promotion of oral and dental health to pre-educational children such as in the education of kindergarten. The aim of this study was to observe the diff erence of the number of caries in the permanent first molar in children of 7-8 years old with pre- elementary and non pre- elementary educational backgrounds. The study was an observational study with cross-sectional approach. The population was elementary school students of 7- 8 years old at Patrang District Jember Regency. The samples were obtained using stratified random sampling. Total sample was 314 students, consit of 157 pre-elementary (kindergarten) and 157 non pre- elementary. Data was analyzed using MannWhitey Test. The result showed that caries without experience of permanent first molar in the children of 7- 8 years old with was 0.07 pre-elementary and 0.44 pre- elementary. It can be concluded that there was a significant differencebetween caries experience of the permanent first molar in the children aged 7-8 years old with and without pre-elementary educational backgrounds .

EFEK MENGUNYAH PERMEN KARET YANG MENGANDUNG SUKROSA, XYLITOL, PROBIOTIK TERHADAP KARAKTERISTIK SALIVA: EFFECT OF CHEWING GUM CONTAINING SUCROSE, XYLITOL AND PROBIOTIC TO SALIVA CHARACTERISTIC Moch. Rodian; Mieke Hemiawati Satari; Edeh Rolleta
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Chewing gum can stimulate saliva secretion and increase saliva component. Chewing gum contains of different substances such as sucrose, xylitol and probiotic. The purpose of this study was to analyze the flow rate, volume, viscosity, pH and the number of Streptococcus mutans colonies before and after chewing gum containing sucrosa, xylitol and probiotic. The research was a quasi-experimental research using a random sampling method. Sample was 30 preclinical students of the Faculty of Dentistry, Padjadjaran University. To analyze the difference of characteristic of saliva used Student t test and one way Anova. The results showed that after chewing gum there were significants increasing of saliva volume, saliva flow rate and saliva pH, and significant decreasing of saliva viscosity and the total Streptococcus mutans colony. The difference of the increasing saliva volume, flow rate, pH and de creasing of total Streptococcus mutans colony and viscositas saliva Statistically there were no differences (p>0,005). In conclusion, chewing gum can increase the saliva volume, flow rate, and decrease the saliva viscosity and the total Streptococcus mutans colony.

HYPERDONTIA IN CHILDREN AT THE PAEDIATRIC DENTAL CLINIC: HIPERDONSIA PADA PASIEN KLINIK GIGI ANAK S.Nagarajan M. P. Sockalingam; Alida Mahyuddin; Wong Ling Siew; Zuryany Mohamed Zahid
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

This was a retrospective study that looked at the prevalence, characteristics, gender predilection and common problems associated with supernumerary teeth found in children at the Paediatric Dental Clinic, National University of Malaysia (UKM). Four hundred ninety clinical records of children with relevant radiographs, mean age of 8 years (range 6-12 yearsold) were analyzed. The result showed that the prevalence of supernumerary teeth was found to be 3.1%. One hundred percent of the supernumerary teeth were found among patients of Mongoloid descendents (Malay and Chinese). Males were more frequently affected than females with a ratio of 4.1:1. Of the 22 supernumerary teeth found, 75.0% of the teeth were unerupted, 91.0% were located at the anterior maxillary region, 77.3% were conical in shape and 72.7% were of mesiodens type. Uneruption of permanent tooth and crowding were the common problems associated with the supernumerary teeth. All patients with supernumerary teeth were in the mixed dentition stage. In conclusion, majority of supernumerary teeth found were unerupted, located at the anterior maxillary region, mesiodens in conical shape and commonly seen in Mongoloid males.

EFFECT OF CHITOSAN ON PROTEIN CONTENT IN THE MEDIUM CULTURE OF OSTEOBLASTS EXPOSED TO OXIDATIVE STRESS: EFEK KITOSAN TERHADAP KANDUNGAN PROTEIN DALAM KULTUR MEDIUM OSTEOBLAS YANG DIPAPAR DENGAN STRES OKSIDATIF Shanty Chairani; Sri Utami; Dewi Fatma Suniarti
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Chitosan is a derivative of chitin which has potential for use in bone regeneration and has been reported can stimulate bone formation. Oxidative stress as one cause of bone damage, was found increased in osteoporosis, periodontitis and arthritis. One of the species oxygen reactive (ROS), hydrogen peroxide, has been reported can inhibit osteoblast proliferation. This study was aimed to investigate the effect of various chitosan concentrations on protein content in the culture medium of human osteoblast-like cell line, MG 63, which was exposed to hydrogen peroxide. MG 63 cells were exposed to various chitosan concentrations (% w/v) 0.1, 0.2, 0.4, and 1.6%. Culture cells without chitosan were used as a control. Cells were growth with Î±-MEM medium (37ÂºC, 5% CO2) until they became confluent, then they were exposed to hydrogen peroxide for 4 hours. The protein content in the culture medium was measured by using Bradford protein assay at 655 nm wavelength. The result showed that hydrogen peroxide decreased protein concentration in the medium culture compared with group without hydrogen peroxide. Treatment group with chitosan concentration 0.4% and 1.6% exhibited a significant increasing of protein concentration in osteoblast culture medium compared with control. In conclusion, in osteoblast culture medium chitosan can inhibit the decreasing of total protein concentration which was caused by oxidative stress.

MATRIX METALLOPROTEINASE-8 GENE EXPRESSION IN GINGIVAL CREVICULAR FLUID OF PATIENTS TREATED WITH REMOVABLE ORTHODONTIC APPLIANCES: EKSPRESI GEN MATRIKS METALOPROTEINASE- 8 DALAM CAIRAN KREVIKULER GINGIVA PASIEN YANG DIRAWAT DENGAN PIRANTI ORTODONTI LEPASAN Susilowati; Imam Mudjari
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Orthodontic treatment with removable appliance produces structural and biochemical changes and breaking the balance between the synthesis and breakdown of the periodontium collagen. MMP- 8 plays an important role in the remodeling of periodontal ligament during orthodontic movement. The purpose of this study was to observe the MMP-8 gene in the gingival crevicular fluid (GCF) of patients with removable orthodontic appliance. It is expexted that the result can be used as a reference to decide the proper time for finger spring to be reactivated. A sample of 8 patients wearing removable orthodontic appliances was obtained. The finger springs were activated with 75 grams of force to produce canine distalization. GCF samples were collected from the distal side of upper canines before force application, 1, 2, 3, and 4 weeks after application consecutively. The sample was analyzed by using RT-PCR. Statistical analyses used were univariate analysis and Mann- Whitney U test. The result showed that the expression of MMP-8 in the GCF at t0 was 28.1% but the force application elevated its expression to 62.5 % at t1, and then decreased continously at t2 (37.5%), t 3(34.4%), and to t 4 (31,3%) . There was no statistical significant difference of MMP- 8 gene expression between and to t 2. Inconclusion, the highest level of MMP-8 gene expression due to orthodontic forces of removable appliance was happenend in the first week, but it declined continously in the following weeks. The proper time to reactivate the finger spring was 2 weeks after application.

EKSPRESI INTERLEUKIN -1 ALFA (IL-1Î±) PADA HIPERSENSITIF KONTAK AKIBAT TONGUE PIERCING: INTERLEUKIN - 1 ALFA EXPRESSION IN CONTACT HIPERSENSITIVITY CAUSED BY TONGUE PIERCING Ananta Herachakri; Feby Aryani; Afrini Puspita; Hendri Susanto; Sri Hadiati
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Tongue piercing is increasingly used by teenagers lately. Studies have reported the tongue piercing's side effects on oral health such as poor oral hygiene, too th and periodontal destruction and taste bud destruction. Tongue piercing may cause hypersensitivity which may be caused by the components of tongue piercing. Interleukin (IL)-1Î± expression may increase in sensitization and elicitation phase of contact hypersensitivity. The aim of this study was to know the IL-1Î± expression induced b y tongue piercing. This study used fifteen two-months year old male Wistar rat (Rattus norvegicus) which was divided into two group, control group treated by vaseline (I) and treatment group. Treatment group divided into three groups, named tongue piercing treatment until sensitization phase (IIA), elicitation phase for 24 hours (IIB), and 48 hours (IIC). Inteleukin- 1Î± expression using immunohistochemistry (IHC) staining from tissue specimen was taken from the tongue. The result of this study showed that IL- 1Î± expression in sensitization phase was higher than elicitation phase and was a significant difference of IL-1Î± expression between sensitization and elicitation phase (p<0,05). LSD test result showed there was a significant difference between sensitization phase and 24 hours elicitation phase, between sensitization phase and 48 hours elicitation phase. In conclusion, there was a difference of IL-1Î± expression between sensitization and elicitation phase of contact hypersensitivity induced by tongue piercing.

RELATIONSHIP OF SALIVARY PEROXIDASE ACTIVITY TO CD4+ LEVEL IN HIV/AIDS PATIENTS: HUBUNGAN AKTIVITAS PEROKSIDASE SALIVA TERHADAP TINGKAT CD4 PASIEN HIV/AIDS Febrina Rahmayanti; Yuniardini S Wimardhani
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Human whole saliva contains peroxidases (human peroxidase and myeloperoxidase) which are part of the innate host defence mechanism in oral cavity. The primary function is to catalyse the oxidation of thyocyanate, in the presence of hydrogen peroxidase, that resulting in the end product of wide antimicrobial activity. Patient with HIV/AIDS is often decreased in salivary flow rate, and this condition may also change the salivary composisition. These changes are contributed in oral lesions of HIV/AIDS patients. The aim of this study was to evaluate the salivary peroxidase activities of HIV/AIDS patients. The study involved 98 patients with HIV/AIDS of whom 55 were men and 43 were women. Unstimulated whole saliva was collected and all the patients received a complete oral examination. The saliva samples were analysed with Bradford assay (with Bradford reagent) and salivary peroxidase measurement with microplate reader and read at 450 nm wave length. The results showed that the mean concentration of total salivary protein and peroxidase in this study population was 1169Âµg/ml (SDÂ±186.1) and 6.101unit/mg Protein (SDÂ±1.26) respectively. It showed a tendency that the lower the CD4 level, the higher the total protein concentration as well as salivary peroxidase activity, however the difference was not statistically significant (p>0.05). Patientsâ€™ data on age, sex, salivary flow rate and pH did not significantly influencing the total salivary protein concentration as well as the peroxidase activity (p>0.05). This study showed that salivary peroxidase activity have a correlation with the total salivary protein concentration. There was a tendency of higher total protein concentration and salivary peroxidase activity found in patients with lower CD4 counts but in those with more acidic saliva. In conclusion, salivary peroxidase activity have a correlation with the total salivary protein concentration in HIV/AIDS patients.

PENGARUH MEROKOK TERHADAP PERTUMBUHAN BAKTERI STREPTOCOCCUS MUTANS DALAM SALIVA: EFFECT OF CLOVE CIGARETTES ON THE GROWTH OF SALIVARY STREPTOCOCCUS MUTANS Evy Tri Utami; Juni Handajani; Tetiana Haniastuti
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Smoking may increase dental caries prevalence, meanwhile dental caries is primarly caused by Streptococcus mutans. The aim of this research was to investigate the effect of clove cigarette smoking on the growth of salivary Streptococcus mutans. In this cross-sectional study, twenty healthy male subjects aged 17 to 25 years was volunteered to join this study. Those subjects were divided into smoking and non-smoking group. Subjects in the smoking group had smoked regularly 6 to 9 clove cigarettes per day for 25 to 35 months. Both groups were asked not to brush their teeth at night before sample was taken. Before having breakfast in the early morning, unstimulated saliva was collected into a tube. The collected saliva was diluted using potassium phosphate buffer and then cultured on spesific S. mutans media agar (TYS20B agar). Streptococcus mutans colonies were identified as milky white colonies. After three days of anaerobic incubation at 37oC, the S. mutans colonies were counted. Data was analyzed using unpaired studentâ€™s t test. The result showed that S. mutanscolonies in the smoking group was significantly higher than the non-smoking group (p<0,05). This study indicated that smoking could increase the growth of S. mutans in saliva. In conclusion, smoking increase the growth of salivary streptococcus mutans.

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Contact Name
Dr. Wilda Hafni Lubis, drg., M.Si

Contact Email
wilda.hafny@usu.ac.id

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dentika_journal@usu.ac.id

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Location
Kota medan,

Sumatera utara

INDONESIA