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Brigitta Laksmi Paramita
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,193 Documents
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Kandungan Fenol dan Aktivitas Antioksidan Makroalga Bentik Caulerpa racemosa (Forsskal) dari Teluk Hurun, Lampung Joko Santoso; Diini Fitriani; Yusli Wardiatno
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2592

Abstract

Caulerpa racemosa is a green benthic macroalga that mainly grows in tropical regions which is expected to bask of strong ultraviolet radiation from sunlight. This circumstance can cause to increase levels of reactive radical species. To reduce and/or protect, organism like macroalga may change its metabolism and stimulate to produce some active compounds, therefore, tropical macroalgae are estimated possessing a large number of active compounds such as antioxidant. In this experiment, edible green benthic macroalga Caulerpa racemosa grown in different water condition were used. The content of total phenol and antioxidant activity of ethyl acetate extract were performed. Pearson correlation between waters condition and antioxidant activities i.e. total phenol and DPPH inhibition were also analyzed. Caulerpa racemosa grown in station 2, exposed in strong radiation from sunlight, had the highest content of total phenol and percentage of DPPH inhibition, with their values were 12.60% and 46.43% respectively. Sunlight intencity in waters had strong positive correlation to the total phenol content and reducing activity of DPPH, however, parameters of nitrate and ammonia had strong negative correlation.
Purifikasi Kolagenase dari Usus Bandeng (Channos channos, Forskal) Tatty Yuniarti; Tati Nurhayati; Agoes Mardiono Jacoeb
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2593

Abstract

Collagenase was purified from intestines of milkfish (Chanos chanos, Forskal) by extraction,ammonium precipitation, ion exchange chromatography on DEAE Sephadex A-50 and gelfiltration on a Sephadex G-100 column. The purification and yields were 114.731 fold and1.26% when compared to those in the starting-crude extract. The molecular mass of milkfishserine collagenase was estimated to be 14.63 kDa and 27.46 kDa.
Kepekaan Cacing Laut Ophryotrocha diadema (Polychaeta: Dorvilleidae) terhadap Cemaran Metil Merkuri (MeHg) Markus Talintukan Lasut; Henneke Pangkey
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2594

Abstract

Susceptibility of the marine polychaete Ophryotrocha diadema (Polychaeta: Dorvilleidae) towards the neurotoxic methyl mercury (MeHg) contamination was studied in an experimental chamber, which was aimed to assess and compare the susceptibility level of the organism based on its generations (F0, F1, F2, and F3). Seven variables of growth and reproduction aspects were applied as indicators in this study; they were: 1) individual growth, 2) first time the egg laid, 3) number of eggs per individu, 4) number of eggs per egg mass, 5) number of eggs to larva per egg mass, 6) number of mortality per egg mass, and 7) reproductive potential. Observation was conducted on the treatment (MeHg in concentration of 0,00025 ppb) and the control (no MeHg) to each of the generations (F0, F1, F2, and F3). Data obtained were analysed for average and standard deviation. Comparison of susceptibility within the generations was calculated using the variable of reproductive potential. The results showed that there were differences between the treatments and the control for all of the variables. Comparison on the susceptibility of the polychaete within the generations to MeHg contamination was F0<F1=F3<F2. It was concluded that the F2 generation had the highest susceptibility among the others.
Struktur Komunitas Moluska Bentik di Perairan Sekitar PLTU Grati, Pasuruan, Jawa Timur Ucu Yanu Arbi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2595

Abstract

Observation on marine benthic molluscs around PLTU Grati waters in Pasuruan was conducted on May and December 2004. The aim of this study was to determine condition and community structure of benthic molluscs living in those areas. Samples were collected using Eckmann grab and sieved through 0.5 mm mesh-sized. The number of molluscs species is 28 species, consisting of 16 species class of gastropoda and 12 species class of pelecypoda. A diversity index (H) ranged 0.845 to 1.158, an evenness index (J) was 0.856 to 1 and a richness index (D) was 19.562 to 31.949. Littorina undulata (Littorinidae) was a dominant species and was relatively widely distributed. In general, this result showed that around PLTU Grati waters had a relatively low benthic molluscs diversity.
Keanekaragaman Lumut di Kawasan Cagar Alam Dungus Iwul, Jasinga, Jawa Barat Florentina Indah Windadri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2596

Abstract

Exploration and collection of the mosses species in Dungus Iwul Nature Reserve were conducted. Thirty eight species including 26 genera and 11 families were recorded. Three species had categories as endemic species i.e Fissidens teysmannianus and Orthomnion javense as endemic species in Java and Hyophila javensis as endemic species in Malesia. Ctenidium lychnites was recorded also as new species record in Java.
Penempelan Larva Siput Laut (Haliotis varia) : Suatu Percobaan di Laboratorium Medy Ompi; Nickson Kawung; Calvyn Calvyn; F. A. Sondak
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2597

Abstract

Settlement of abalone (Haliotis varia) focusing on substrate quality and larval behavior were studied. Four types of substrate, shell and mucous, algae Gracilaria sp and Halimeda sp, and plastic petri-dish were used to test whether the larva might respond and settle on those substrates. At the same time, the mortality of larvae was also determined during the settlement. Without petri-dish, substrate of shell and mucous, algae, as well as polyvinyl chloride (PVC) plate were tested to find out substrate preferences when settlements occured. The result showed that variation of substrata, shell and mucous, algae and plastic “petri-dish” possessed a stimuli responded by larva to settle. Shell and mucous as well as substrate algae were attractive among of substrata, since high of settlement on it were recorded. High larval mortality on shell and mucous as well as on substrata of algae were recorded. Substrate of shell and mucous and alga were favorable to substrata when settling. Several factors affecting settlement of larval abalone were discussed.
Enkapsulasi dan Regenerasi Kalus Embriogenik Mangga (Mangifera indica L.) Kultivar Bapang dan Gadung 21 Tri Muji Ermayanti; Robertus Nugroho; Hamim Hamim
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2598

Abstract

The mother plant and genetic variability of Indonesian mango need to be conserved. Encapsulation is one of in vitro conservation used for many plant species. The aim of this research was to study the regeneration of encapsulated mango cultivar Bapang and Gadung 21 embryogenic callus after storage at -14, 3−5, and 26−27oC for 0, 1, 2, 4, and 8 weeks. Embryogenenic callus was treated with 3% Na-alginate (in liquid 3M), then it was dropped into 100 mM CaCl2. Encapsulated callus beads were dehidrated and stored at -14, 3−5, and 26−27oC for 0, 1, 2, 4, and 8 weeks. After storage, the callus was cultured in 3M medium containing 2,4-D at 0, 1, and 2 mg/l for the regeneration. The results showed that after 8-week storage, callus of Bapang culticar was more viable (67.3%) and resulted more number of somatic embryos (191.6%) than Gadung 21 cultivar. The callus which was cultured in 3M medium without addition of 2,4-D was more viable (20.9%) and had more number of somatic embryos (1062.5%) than that which was cultured on medium containing 2,4-D. After 2-week storage, callus had viability of 7.6%. No storage callus formed more somatic embryos than storage callus. Storage at 26−27oC gave higher viability as well as higher number of somatic embryo than stored at -14 and 3−5 oC. The callus did not regenerate into shoots after storage at -14oC. Embryogenic callus could be stored at 3−5 and 26−27oC for 4 weeks.
Pengaruh Ekstrak Daun Nerium oleander L. terhadap Mortalitas dan Perkembangan Hama Spodoptera litura Fab. Heri Prabowo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2599

Abstract

Research was conducted at the Laboratory of Entomology, Tobacco and Fiber Crops Research Center from June to July 2009. Tests were conducted on the larvae of Spodoptera litura second instar. Concentration of leaf extract of N. oleander used was 0; 2x105; 3x105; 4x105; and 5x105ppm. Treatment was done by spraying method in the spray chamber. Treatment was arranged in a completely randomized design (RAL) with 4-time repetition. Each repetition used 25 larvae. Observed parameters included the death for every 24, 48, 72, and 96 hours; length, width, and weight of larvae after 96 hours after treatment. By 96 hours after treatment with the concentration of leaf extract 0; 2x105; 3x105; 4x105; and 5x105ppm it was capable of causing mortality S. litura, respectively for 0; 48.33; 53.33; 66.67%, dan 76.67%. By 96 hours after treatment extract concentration required to cause mortality S. litura was 25, 50, and 95% respectively of 2.35x105, 4.44x105, and 9.49x105ppm. After 96-hour treatment the concentration of leaf extract 0; 2x105; 3x105; 4x105; and 5x105ppm could cause the length, width, and weight of S. litura, respectively for 0.89 mm; 0.91 mm; 0.93 mm; 0.94 mm; 0.95 mm; 0.15 mm; 0.16 mm; 0.17 mm; 0.18 mm; 0.19 mm, 0.011 g; 0.012 gr; 0.013 g; 0.014 g and 0.016 gr. The use of N. oleander leaf extract caused mortality of S. litura between 13.33−76.67% at 24 to 96 hours after treatment. Leaf extract of N. oleander potentially caused mortality S. litura. The use of this leaf extract could also inhibit the length, width, and weight of larvae.
Peran Ovicidal Herbal Serbuk Biji Pepaya (Carica papaya L) Matang Terhadap Telur Cacing Ascaris suum Ida Ardana
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2600

Abstract

An in vitro experiment to determine the effect of ground mature papaya seeds on characteristics of Ascaris suum eggs was conducted employing a Randomized Block Design. Four doses of the ground preparation – 0 mg (as control), 285 mg, 570 mg, and 855 mg- in 40 ml of physiological saline solution – were used to soak 100 eggs. Six replications were made. Worm eggs were obtained from the uteruses of ascariasis pigs slaughtered at the local abattoir. The eggs characteristics observed after application of the treatment were the embryo formation ability and the damage of egg cell layers. Statistical analysis was applied to the data collected using Analysis of Variance followed by Least Significance Test. The results showed that the ability of treated eggs to form embryos was significantly lower than that of control. Such a decrease in embryo formation ability could be related to the formation of granule (albumen coagulation) in the egg cell layers after the treatments. All treated eggs possessed such granules.
Penggunaan Operkulum dalam Penentuan Umur pada Rhinoclavis sinensis Gmelin 1791 (Gastropoda: Cerithiidae) Felicia Zahida; Jusup Subagja
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2601

Abstract

Age determination of the sample specimen is very important for population dynamic research of R. sinensis (Gastropoda : Cerithiidae). Operculum is a “hard” part stick on the dorsal portion of the foot of gastropods. Operculum appeared on the day of the snails are born and have their own specific shape, size, and materials composition. The aim of this research was to describe the use of operculum in age determination of R. sinensis. The method used in this research was: the operculums were soaked into saturated sodium hydroxide, washed and mounted them in Canada balsam, and observed using binocular microscope. Regression analysis was used to find the relationship between length of operculum to length of shell, and number of adventicious layers of operculum to length of shell. The research resulted that the length of operculum was comparable with the shell length, with the regression line of Y=0.113X +1.898 (R2=0.857). The growth of adventitious’ layers of the operculum coincided with the growth of the shell that in the second year, the growth of adventitious layers was two layers a year. At the age of three and four, the growth was one layer a year. During the fifth, sixth and seventh years, the growth was only a layer within three years. The regression line was Y=0.168X (R2=0.872).

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