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Brigitta Laksmi Paramita
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,193 Documents
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Variasi Genetik pada Rusa Sambar (Rusa unicolor) di Penangkaran, Kabupaten Penajam, Kalimantan Timur Wirdateti Wirdateti; Bram Brahmantyo; Gono Semiadi; Andi Reksodihardjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2602

Abstract

Sambar deer (Rusa unicolor) is the largest Indonesian deer species. The largest population of captivation is located at Penajam district, East Borneo (UPTD). First population was introduced in 1990 with four individuals. The use of molecular marker was aimed to identify and characterise the level of genetic diversity within the UPTD population as well as to identify a possible of botolneck population genetic status. This study discussed the relevance of the result for management purposes of captivation. The results indicated that sambar deer populatin (n=38) had an average genetic distance (d) in population as 0.006 with nucleotide diversity (π) being 0.0159). A total of 43.48% of the population was homogeneous that showed no nucleotide differences among individuals.
Pengaruh Pupuk Nitrogen dan Ethephon terhadap Pertumbuhan, Pembungaan dan Hasil Padi Lokal (Oryza sativa L. cv. Rojolele) Diah Rachmawati; Maryani Maryani; Tri Setyaningsih
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2603

Abstract

Physiological studies on Rojolele rice were conducted after the application of nitrogen fertilizer and ethephon at different doses. Application of nitrogen fertilizer is one of the most important measurements to improve plant growth and yield of rice. Ethylene which has flowering initiate roles are induced by ethephon. The objective of this study was to analyze the effect of nitrogen fertilizer and ethephon on growth, flowering and yield of rice (Oryza sativa L. cv. Rojolele). The experiment was carried out using Complete Randomized Design with 2 factors, which each factor consisted of four levels. The first factor was doses of urea as nitrogen source 0, 50, 100 and 150 kg ha-1. The second factor was doses of ethephon 0; 100; 200 and 400 mg l-1. Measured parameters were plant height, tiller number, plant biomass, root shoot ratio, flowering time, yield and grain size. The application of nitrogen fertilizer improved the plant growth of Rojolele rice. Plant height, tiller number, plant biomass, panicle length, and number of grains increased by the increasing doses of nitrogen, but this factor did not significantly affect on grain size. Ethephon application reduced plant height and delayed flowering time. However, it simultaneously increased tillers when compared at the same N dose. Interaction between nitrogen fertilizer and ethephon gave significantly effects on the plant height and number of grains per panicle, but there were no significant effect on the rice grain size.
Pola Pergerakan Perilaku Agonistik Lobster Air Tawar Cherax quadricarinatus (Von Martens, 1868) Tarjono Tarjono; Mufti P. Patria
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2604

Abstract

This research on movement patterns of agonistic behavior in crayfish Cherax quadricarinatus was conducted at laboratory and used an aquarium size 25 cm x 10 cm x 15 cm. The patterns of agonistic behavior were recorded by using a CCD and digital camera. The recorded data were analyzed using The Adobe Premier computer program and using Jaccard similarity to solve the reciprocal movement patterns. Clustering based on the UPGMA (Unweighted Pair Group Method Average) indicated that the dissimilarity value was about 0,98 and the moving pattern of the agonistic behaviors were grouped into five groups, namely: 1) threatening; 2) arranging the strategy; 3) testing the enemies capability; 4) fighting; 5) avoiding and defending.
Analisis Keanekaragaman Isolat Bacillus thuringiensis yang Patogenik terhadap Serangga Hama Kubis (Crocidolomia binotalis) dengan Pendekatan Sistematika Numerik Christina L. Salaki; Jesmandt Situmorang; Langkah Sembiring; Niken Handayani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2605

Abstract

Diversity of B. thuringiensis (Bt.) isolates pathogenic to C. binotalis was determined by using Numerical Systematic Method. Ten isolates were taken to represent 34 pathogenic isolates along with two reference strains namely B. thuringiensis serovar kurstaki and B. thuringiensis serovar israelensis. The test isolates were examined for 89 phenotypic characters by using convensional method for colonial and cell morphology (37 characters) as well as physiological characteristics (3 characters) but biochemical characterization (49 characters) was conducted by using commercial API-50 CHB procedures. All phenotypic characters existed in one of two mutually exclusive states and were either scored plus (1) of minus (0). The binary data were prepared in Programmer’s File Editor (PFE) software. The data then were analysed by using the Multi Variate statistical Package (MVSP) Plus-Version 3.1 using the Simple Matching Coefficient (SSM). Clustering was achieved using the UPGMA algorithm. The results were presented as dendrograms. It was obtained that the test isolates were clearly assigned to two distinct multimembered clusters defined by 79.6 similarity level (S-level) in the SSM, UPGMA analysis. The two distinct clusters represented by each of two widely known different group of Bt. strains, namely serovar israelensis and serovar kurstaki. The first cluster contained reference strain of B. thuringiensis serovar israelensis, and two of the isolates (Slk2.3, and YPPA1) and the second cluster contained another reference strain of B. thuringiensis serovar kurstaki, and 8 of the isolates. Therefore, it strongly suggested that the application of numerical-fenetic analysis could provide a tool to unravel the strain diversity belong to B. thuringiensis.
Interpretasi Genetik Pola Pita Isozim pada Cherax quadricarinatus Hasil Budidaya di Purwokerto dan Bogor Dian Bhagawati; Muh. Nadjmi Abulias
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2606

Abstract

A study on genetic interpretation of isozyme band patterns of C. quadricarinatus cultured in Purwokerto and Bogor showed the good visualization of aspartat transaminase, alkohol dehidrogenase, malat dehidrogenase, esterase and acid phosphatase. Twelve loci resulted low level of polymorphism (P= 0,25) and also low valve of heterozygosity (H= 0,09). It indicated that the genetic diversity of both populations was low.
Khemotaksis Rhizobakteri Osmotoleran pada Rizosfer Tanaman Kacang Hijau (Vigna radiata, L.) Yekti Maryani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2607

Abstract

This research has an objective to study chemotaxis of osmotolerant rhizobacteria strains Al-19 dan M7b in greenpea plant. These isolates were used to inoculate greenpea plant. The study on chemotaxis of osmotolerant rhizobacteria was conducted by CFU method in order to count the number of osmotolerant rhizobacteria AL-19 dan M7b in rhizosphere. Visualization of those isolates on root surface used fluorescence microscope and agglutination reaction with exudates of greenpea root. Result of the study showed that both isolates of osmotolerant rhizobacteria Al-19 dan M7b were found in rhizosphere of greenpea with low-density. Simple carbohydrate is substrat that is essential for rhizobacteria to grow thus the chemotaxis of both rhizobacteria is considered as metabolism - dependent. It means that it is not only as digested material but also function as affinity substance. These isolates gathered on the root surface weakly and did not make glutination reaction. This condition indicated that these isolates could not form colony on root surface of greenpea.
Potensi Mangrove dan Manfaatnya bagi Kelompok Etnik di Papua Agustina Arobaya; Freddy Pattiselanno
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2608

Abstract

significant contribution to those who living in and around mangrove forests. Like any othercommunities who occupied areas surrounding forest sites, Papuan ethnic groups really connectand integrate with the forest environment such as mangrove forests. This paper is a review ofrelated literatures that compiled from different research which have been conducted inUniversitas Negeri Papua (UNIPA) on utilization of mangrove by local ethnic groups and its rolefor other life biota in Papua. The results indicated that mangrove plays a very important rolefor local tribes in Papua as food sources, construction materials and source of energy,traditional medicine sources and wildlife habitat that are usually consumed by the communities.Ecology-based tourism or ecotourism as one leading sector of mangrove forests in Papua,though has a good potential, has not been optimizedly used.
Skrining Senyawa Antibakteri dari Beberapa Jenis Rumput Laut Coklat (Phaeophyceae) dari Pantai Drini, Daerah Istimewa Yogyakarta Boy R. Sidharta; P. Kianto Atmodjo; E. Mursyanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 1 (2008): February 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i1.2614

Abstract

This present research was trying to determine the antibacterial compounds from several brown seaweeds from Drini beach. Previous studies have shown that several seaweeds from this beach were proved to have antibacterial activities. Therefore, it needs to be studied whether the extracts of several brown seaweeds utilizing three (3) kinds of extraction solutions such as aquadest, ethanol, and hexane, will also show antibacterial activities. The three solutions were chosen based on the difference of polarization activities. Research design utilized was randomized complete block design and the data compiled was treated by Anova and followed with Duncan’s Multiple Range Test (DMRT) with degree of confidence 95%. Microbes utilized in this research were Escherichia coli and Bacillus subtilis, that were common pathogenic bacteria known to human and animals. The research found six (6) species of brown seaweed during the research period, i.e. Sargassum dotyi, S. ohnoi, S. abbottiae, S. bataanense, Caulerpa prolifera, and Laurencia papillosa. This present research also revealed that ethanol was the best extraction solution to extract the secondary metabolite from the brown seaweeds found. The best antibacterial activities was shown by S. dotyi extract’s to inhibit the two (2) microbes used in the research compared to other seaweed extracts. However, this result was still low and significantly different compare to pure antibiotics utilized, i.e. penicillin and streptomycin.
Pengaruh Heat-Moisture Treatment dan Penambahan Natrium Tripolifosfat terhadap Kualitas Mi instan dari Pati Ubi Jalar (Ipomoea batatas (L) Lam) Chatarina Lilis Suryani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i2.2615

Abstract

The objective of the present study was to evaluate the effect of heat-moisturetreatment (HMT) and sodium tripolyphosphate (STPP) addition on the quality ofinstant noodles from sweet potato starch. The diversification of raw material used innoodle production often necessitates processing modifications. Heat-moisturetreatment was applied to native sweet potato starch (HMTSPS) in three conditions(1100C; 10, 20 and 30 min) and STPP was added in three level (0,60; 0,75 and 0,90%).HMTSPS, STPP, water, lecitin and CMC were mixed. The mixture was pelleted,steamed and extruded. The extruded noodles were dried using a microwave oven. Thenoodles were evaluated both when dry and after cooking. The HMT method andSTPP addition improved noodle quality. Swelling index, solid loss and cooking loss ofthe noodles was decreased, while the texture and the elastisity of the noodles washigher. But noodles made with added of STTP more than 0,75% had low elastisity.The best instant noodle prepared by HMT method for 20 min and added of STTP0,75%.
Kualitas Keripik Bengkuang (Pachyrrhizus erosus (L.) Urb.) dengan Variasi Konsentrasi Ekstrak Bunga Rosela (Hibiscus sabdariffa Linn.) Martina Setyasih; F. Sinung Pranata; Ekawati Purwijantiningsih
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 1 (2008): February 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i1.2616

Abstract

Yam bean chips have crispy texture and sweet taste. In this research, rosella extract is added in the mold of yam bean chips as natural preservaties, fiber and sources anthocyanin. The aim of this research is to know the effect of various consentrations of rosella extract on chips qualities. Research design utilized was randomized complete factorial design and the data compiled was treated by Anova and followed with Duncan’s Multiple Range Test (DMRT) with degree of convidence 95%. Chips with 0,67% rosella extract added has longer shelf life than chips with out rosella extract added. This Chips contain 0,59% water, 2,6% protein, 38,84% fat, 3,69% ash, 54,16% carbohydrate, 1,17 mg/100g ascorbic acid, 1,4 mg/l anthocyanin, 1,17% acid total, 86,74%fiber crude, 53,47 level of lightness and total microorganism 30 CFU/G.

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