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Brigitta Laksmi Paramita
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,193 Documents
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Pemanfaatan Ekstrak Daun Kecubung (Datura metel L.) Sebagai Pembius Ikan Koi (Cyprinus carpio L.) Pada Saat Pengangkutan Sapto Eko Hariyanto; F. Sinung Pranata; Yuniarti Aida
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 1 (2008): February 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i1.2617

Abstract

Either decorative fish or consumption fish would be costlier in price if sold in a state of life. However both having the same problems that is death at the time of transportation process. From former research has been obtained information that transportation of fish in a state of life can be done by using material anaesthesia either experiencing and also artificial. Usage of chemical material as fish anaesthetic is felt able to give unfavourable effect to quality and fish health. Purpose of this research knows concentration of metel thorn apple leaf extract and soaking stripper that is most effective in anaesthesia of fish koi (Cyprinus carpio L.) and knows does metel thorn apple leaf extract can lessen fish mortality koi (Cyprinus carpio L.) at the time of transportation of long distance. Initial concentration which will be applied is 0,4%, 0,1%, 0,06%, 0,03% and 0% as control, because concentration doesn't have an effect on concentration is boosted up to become 0,4%, 0,7% and 1% with soaking stripper 4, 8, 12 hours. Result of research shows concentration of 0,7% and soaking stripper of 8 hour, this time is most effective in anaesthesia of fish koi (Cyprinus carpio L.) because level of survival rate 100% with induction time stripper and recovery time is stripper. Research is continued with test trasportasi compared to control, where fish is not anaesthetized packed into plastic poke and given with pure oxygen like treatment that is usualy is done the fish farmers in process of transportation. Result of this research indicates that, treatment of fish anaesthetized with metel thorn apple leaf extract level of pass of life reachs 91,667% while controlling 66,667%. From result of inferential research that usage of metel thorn apple leaf extract at the time of transportation of proven long distance can lessen fish mortality koi (Cyprinus carpio L.).
Kualitas, Kemampuan Implantasi dan Viabilitas in-vivo Embrio Mencit (Mus muculus) Galur Swiss Webster Setelah Pembekuan Dengan Metode Vitrifikasi Madihah Madihah; Hartanti Kusumaningtyas; Arief Boediono; Sony H. Sumarsono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i2.2618

Abstract

Reproductive technologies including in vitro fertilization (IVF), embryo manipulation, gamete and embryo freezing, thawing and embryo transfer were rapidly developed. Vitrification is an embryo freezing technique that is the most developed. In this experiment, we vitrified mouse embryos and then examined the embryos i.e: (i) the quality of the embryos after thawing, (ii) the implantation rate of the embryos and (iii) viability of the embryos in vivo. Morulae and blastocycsts were collected from female mice that were pregnant a day 3,5. The embryos were equilibraten in mPBS +10% etilene glycol. Vitrification was carried out by using VABEDS medium, containing 6-10 embryos that were dropped into a tip of a straw, then frozen in liquid nitrogen for 24 hours. Thawing was carried out by flushing the embryos using mPBS suplemented with 0.5, 0.25, 0.1 and 0 M sucrose. After being incubated in M2 medium at 37oC for 1-2 hours, the recovery embryos were then transferred into the uteri of day 2.5 of pseudopregnat females. The females were then sacrificed at day 16 of gestation and the total implantaion, total life and death fetuses, as well as resorpted embryos, were taken as data. The results showed that vitrification significantly (p<0,05) reduced the quality of the embryos, as well as their implantation rate and the viability of the fetuses, which may be caused by the unoptimal combination of the cryoprotectant in the vitrification medium, temperature and exposure time during vitrification.
Pemanfaatan Kitin Udang Sebagai Penyerap Timbal dan Tembaga pada Limbah Cair Industri Batik di Solo Martinus Dwi Cahyanto; Yuniarti Aida; F. Sinung Pranata
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 1 (2008): February 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i1.2619

Abstract

The waste of shrimps in the form of skin, head, and tail that is disposed from shrimp processing and disposal are usually thrown away with out being processed, therefore the wastes may cause environmental pollution, especially from the unpleasant stench. Shrimp wastes contain compounds called chitin and can be utilized to absorb heavy metals which are mainly from industrial wastes. The batik industries in Solo usually utilize synthetic colors, because synthetic colors are cheaper and can make the products look more satisfying. Nevertheless, the liquid wastes from these batik industries are usually still colorful when it is disposed and are usually hard to be degraded by nature. Moreover, these wates contains heavy metals such as lead (Pb) and copper (Cu) that can endanger the human health. This research is conducted in order to notify the performance of chitin in order to absorb heavy metals such as lead (Pb) and copper (Cu). There are five treatments that is conducted in this research by adding chitin powder as much as 0 g (control), 1, 2, 3 and 4 g into the sample of batik liquid wastes. The lead (Pb) and copper (Cu) substance of the sample will then be measured using the AAS (Atomic Absorption Spectrophotometer), then the lead (Pb) and copper (Cu) substance of each treatment will be compared with the control. The result of the research shows that chitin can reduce the content of lead and copper in batik liquid wastes. Based on the research result, it is concluded that the addition of 4 g of chitin powder into the sample of batik liquid wastes can give the most maximum result, i.e. the reduction of the Pb content by 6.138 ppm from the control of 17.773 ppm into 11,635 ppm, and the reduction of the Cu content by 3.779 ppm from the control of 7.903 ppm into 4.104 ppm.
Pengaruh Kombinasi Tapioka dan Tepung Kedelai terhadap Kualitas Sosis Ikan Kakap Putih (Lates calcarifer Bloch) Prima Novi Agatha; Ekawati Purwijantiningsih; F. Sinung Pranata
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 1 (2008): February 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i1.2620

Abstract

Sausage is food product made from mix ture of minced meat (contain meat more than 75%), flour or starch with or without additional spices, and other allowed food additive. Sausage made from meat usually has a higher content of fat, which in many cases usually caused serious health problem. To reduce the fat content in sausages, the researchers try to combine cassava starch with soybean flour. Soybean flour was used in combination with cassava starch because it reduced of cholesterol in blood, reduced oil absorption while frying. Soybean flour is a kind of food material that has higher content of protein but low fat, and it has complete essential amino acids. This research was conducted to find out the influence of cassava starch and soybean flour combinations to produce good quality of seabass sausages. In this experiment, cassava starch and soybean flour combination were as follows 90:10; 80:20; and 70:30. The result showed that the combination of cassava starch with soybean flour increase water content, ash content, protein content and fat content but elasticity and carbohydrate content were decrease.
Produksi Fitoaleksin pada Tusam (Pinus merkusii Jungh. et de Vriese) sebagai Respon Infeksi Fungi Mikorisa S. Widyaningsih; S. M. Widyastuti; Sumardi Sumardi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i2.2621

Abstract

The experiment aimed to detect (1) mycorrhizal infection induced phytoalexins production of the root of tusam and (2) its activity in suppressing a dumping off fungi, Fusarium sp and Rhizoctonia solani. Production of phytoalexins was detected from the extracts of mycorrhizal root of old trees and 4, 6 and 8 week-old seedlings in alcohol using a UV-spectrophotometer. The antifungal activity of phytoalexins was studied by inoculating the pathogenic fungi into mycorrhizal seedlings of tusam. The results showed that the spectra of phytoalexins of extracted mycorrhizal and non-mycorrhizal roots ranged from 203.2 to 204.6 nm. Higher antifungal concentrations of antifungal compound was obtained from the root extracts of old trees and 4 week-old seedlings, indicated by the higher spectrophotometric absorbance (0.315 and 0.324 g-1 root ml-1 ethanol), compared to that extracted from non-mycorrhizal root of the same plant origin. Mycorrhizal roots significantly suppressed the development of Fusarium sp. and Rhizoctonia solani.
Pengaruh Ragi dan Waktu Fermentasi terhadap Produksi Alkohol Secara Fermentasi Berbahan Baku Gaplek Ubi Kayu (Manihot utillisima) P. Kianto Atmodjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 1 (2008): February 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i1.2622

Abstract

The research of the ragi and the fermentation time effect on alcohol production by cassava gaplek has been done. The aim of this research were to find out the best time fermentation and trade mark commercial ragi to produce alcohol. Time fermentation were 1 and 2 weeks. The ragi trade marks were NKL and MK. The result showed that the alcohol production was 15-17% in a week, and 18-20% in two week fermentation. The alcohol quantity which produced by both NKL and MK ragi were not significantly different.
Observations on Natural Foods and Nutrition Content of Critically Endangered Turtle (Leucocephalon yuwonoi) in Central Sulawesi Awal Riyanto; Wirdateti Wirdateti; Suprayogo Soemarno
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i2.2623

Abstract

Kura-kura Sulawesi (Leucocephalon yuwonoi) adalah satu dari dua jenis kura-kura endemik Sulawesi dengan penyebaran yang terbatas. Sejauh ini data natural historinya sangat minim, padahal sangat dibutuhkan dalam upaya penangkaran satwa yang berstatus kritis IUCN ini. Penelitian ini bertujuan untuk mengungkap tentang pakan di alam beserta kandungan nutrisinya. Survei lapangan dilakukan di kawasan Bangkir dari tanggal 23 Mei sampai 5 Juni 2004 dan di Moutong dari tanggal 11 sampai 19 Oktober 2004. Data pakan di alam diperoleh dari analisis sampel feces dan hasil wawancara kepada para pemburu kura-kura beserta pengumpul. Kepastian nama jenis pakan diperoleh dengan melakukan identifikasi sampel tumbuhan pakan di Herbarium Bogoriense. Data kandungan nutrisi diperoleh melalui analisis proksimat sampel pakan yang terkoleksi selama survei. Hasil penelitian ini menunjukkan bahwa terdapat 32 jenis tumbuhan yang jadi sumber pakan di alam, variasi kandungan nutrisi yang cukup besar, kandungan lemak rendah berkisar antara 0.74 - 8.33% (2.41+2.03) dari berat kering. Berdasarkan dominansi, keberadaan di habitat (multiple season) dan tingginya kandungan energi diduga kuat bahwa Colocasia esculenta, Limnocharis flava and Ipomoea aquatica merupakan pakan utama di alam.
Bahan Tambahan Pangan (Kajian Buku) F. Sinung Pranata
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 1 (2008): February 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i1.2624

Abstract

Perkembangan yang pesat di bidang industri makanan telah membuat Wisnu Cahyadi membuat edisi baru dari buku Bahan Tambahan Pangan. Buku ini merupakan upaya peningkatan buku sebelumnya (edisi pertama). Pada buku edisi kedua ini ada beberapa kata yang direvisi dan penambahan materi baru, khususnya pada lampiran, yaitu tentang Persyaratan Penggunaan Bahan Tambahan Pangan Pemanis Buatan dalam Produk Pangan. Kelebihan buku ini adalah tidak hanya menjelaskan tentang bagaimana cara penggunaan dan persyaratan bahan tambahan pangan, tetapi mencakup analisis dan aspek kesehatan.
Purifikasi dan Karakterisasi Krom Reduktase Bacillus sp LKA9 Alimuddin Ali; Endang Sutariningsih Soetarto; J. Sri Widada
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i2.2625

Abstract

Chromate reductase is one of the potential enzymes for hexavalent chrom detoxification. Most of the enzyme is produced by bacteria, especially Bacillus. The aim of this research was to study chromate reductase activity isolated from Bacillus sp LKA9. Bacillus sp LKA9 was isolated from leather tannery liquid waste and used as a model in the experiment. Bacillus sp LKA9 was isolated through enrichment culture using Salt Base Solution containing 3 mM K2CrO4. Chromate reductase was isolated from bacteria by growing on a liquid medium containing chrom hexavalen (Cr VI) through several steps. The first step of the isolation process was to use the precipitated process using ammonium sulphate (30-80%). The next step, crude enzymes from the first step was partially purified through DEAE-Cellulose of Ion Exchange Chromatography Column. Diphenylcarbazide methods was used to examine the activity of enzyme fractions. The result of the experiment revealed that all protein could be precipitated by ammonium sulphate, and the cytoplasmic fraction at saturation of 50-70% showed high enzyme activity. Purified enzymes showed an increase activity 69,385 times to that of crude enzymes. The enzyme optimal had temperature and pH were 350C and 5; respectively. KM of enzyme was 0,0075 mM, and Vmax was 2500 mol/minute/mg protein. Enzyme activity was not inhibited by Cu2+, but an ion Ag2+ and Hg2+ inhibited the enzyme activity un-competitive. The activity of enzyme was demonstrated on SDS-PAGE by appearing typically band with molecular weight 29,26 kDa, it was assumed as chromate reductase.
Fitoekstraksi Sianida pada Centrosema pubescens Benth yang Tumbuh di Limbah Tailing Terkontaminasi Sianida Fauzia Syarif; Nuril Hidayati; Titi Juhaeti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i1.2626

Abstract

Contamination of mined soil and water affects not only to agriculture system but alsofood chains and epidemiological problems. As soil metal can not be biodegraded,remediation of soil heavy metal risks has been a difficult and expensive goal. Presentlythere are several different strategies available for the clean up and restoration ofcontaminated soils. One approach to minimize risks from some toxic pollutants isphytoextraction using hyperaccumulator plants, known as phytoremediation. In thisresearch Centrosema pubescens was studied to examine its potency ashyperaccumulator against cyanide. Although cyanide is not categorized as heavymetal, its presence is considered as one of important toxic pollutants in theenvironments. Detoxification of cyanide (CN) contaminated soils and waters withplants seems to be a feasible option. Centrosema pubescens which proven tolerant anddominant in the CN contaminated environment was used in this research. The plantswere grown in tailing waste media added by 0, 10, 20 and 30 ppm CN. Different pHlevels were applied, i.e.+ 6 and +5. The results showed that the plants were capable ofgrowing under high level of CN. The results indicated that Centrosema pubescens canbe considered as high tolerance and potentially effective in accumulating CN, i.e up to3.564 ppm in roots and up to 3.564 ppm in shoot with concentration ratio (shoot/root)up to 1.13. The plants also indicated high tolerance by producing high biomass underhigh level of CN, i.e up to 36.517 g (under 20 ppm CN).

Page 79 of 120 | Total Record : 1193

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