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All Journal Biotika: Jurnal Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Khizanah al-Hikmah : Jurnal Ilmu Perpustakaan, Informasi, dan Kearsipan Indonesian Journal of Pharmaceutical Science and Technology Biosaintifika: Journal of Biology & Biology Education Hanifiya: Jurnal Studi Agama-Agama Jurnal Kedokteran Hewan Jurnal Bioteknologi & Biosains Indonesia
Madihah Madihah, Madihah
Unknown Affiliation
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Immunology & microbiology Library & Information Science Medicine & Pharmacology Veterinary

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Reversibility Time in Testicular Damage on Male Wistar Rat after Treatment of Averrhoa blimbi L. Fruits Extract as Antifertility Alipin, Kartiawati; Rochman, Indra A.; Malini, Desak M.; Madihah, Madihah
Indonesian Journal of Pharmaceutical Science and Technology Suppl 1, No. 1 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (561.875 KB) | DOI: 10.15416/ijpst.v1i1.16123

Abstract

Belimbing wuluh (Averrhoa blimbi) fruit is commonly used traditionally as antifertility. The bioactive compounds of A. blimbi have been known could inhibit spermatogenesis and development of testes, as well as decrease the quality and quantity of spermatozoa, thus, caused seminiferous tubular atro- phy. This study aimed to determine the reversibility time in testicular damage after the treatment of A. blimbi fruit extract on male Wistar rat. The method employed a completely randomized design (CRD) consists of nine treatments with three replications each. The treatment of the extract at a dose 1140 mg/ kg BW and control group was given the solvent. After 14 days of treatment, the reversibility time was examined in testis histological section by interval of seven days each until 49 days. The results showed that the treatment of A. blimbi fruit extract caused disruptions in seminiferous tubules, i.e. decreased the number of spermatogonium and spermatid, as well as the diameter of lumen and seminiferous tu- bules. The reversibility time was observing at 21 days after the end extract treatments, by increasing the number of of spermatogonium and spermatid, as well as the diameter of lumen and seminiferous tubules that signi cantly different with infertile rat (p<0.05). In conclusion, the reversibility time was 21 days after the treatment of A. blimbi fruit extract as antifertility. Key words: Averrhoa blimbi, rats, reversible time, tubulus seminiferous
Ethnobotanical Study of Medicinal Plants in Karangwangi, District of Cianjur, West Java Malini, Desak Made; Madihah, Madihah; Kusmoro, Joko; Kamilawati, Fitri; Iskandar, Johan
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.5756

Abstract

The knowledge and usage of plant as medicinal remedy by current generation are not as extensive as previous; therefore, many rural communities with restricted modern medical access still rely on traditional medicine. This paper provides significant ethnobotanical information on medicinal plants in Karangwangi Village of Cianjur District, West Java Indonesia. This study aimed to identify plants collected for medical purposes by the local people as well as to document the local names, uses, preparation, and location of these plants. Ethno botanical data was recorded by opting people participation and key informant approach involving semi-structured interviews, group discussions and filling of questionnaires. The results showed a total of 114 medicinal plants belonging to 50 families were identified. Zingiberaceae was the most-frequently cited (nine species), followed by Asteraceae, Euphorbiaceae, and Fabaceae (seven species each). The most-used plant parts were leaves (51.8%), followed by stems (22.9%) and the most common preparations were decoction, poultice and squeezed. Most of the plants were obtained from the house-yard and total of 30 medicinal uses were recorded. The ethnobotanical result documented in this study showed that this area is rich in medicinal plants and these plants are still commonly used for medicinal purposes among the people in their daily lives. Ethnobotanical heritage should be preserved, however, there is a gradual loss of traditional knowledge about these plants in new generation. Further, the findings can be used as baseline information for further scientific investigation for analyzing phytochemical, pharmaceutical and other biological activities for future drug discovery.
KUALITAS, KEMAMPUAN IMPLANTASI DAN VIABILITAS IN-VIVO EMBRIO MENCIT (MUS MUCULUS) GALUR SWISS WEBSTER SETELAH PEMBEKUAN DENGAN METODE VITRIFIKASI Madihah, Madihah; Kusumaningtyas, Hartanti; Boediono, Arief; Sumarsono, Sony H.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.641 KB) | DOI: 10.24002/biota.v11i2.2618

Abstract

Reproductive technologies including in vitro fertilization (IVF), embryo manipulation, gamete and embryo freezing, thawing and embryo transfer were rapidly developed. Vitrification is an embryo freezing technique that is the most developed. In this experiment, we vitrified mouse embryos and then examined the embryos i.e: (i) the quality of the embryos after thawing, (ii) the implantation rate of the embryos and (iii) viability of the embryos in vivo. Morulae and blastocycsts were collected from female mice that were pregnant a day 3,5. The embryos were equilibraten in mPBS +10% etilene glycol. Vitrification was carried out by using VABEDS medium, containing 6-10 embryos that were dropped into a tip of a straw, then frozen in liquid nitrogen for 24 hours. Thawing was carried out by flushing the embryos using mPBS suplemented with 0.5, 0.25, 0.1 and 0 M sucrose. After being incubated in M2 medium at 37oC for 1-2 hours, the recovery embryos were then transferred into the uteri of day 2.5 of pseudopregnat females. The females were then sacrificed at day 16 of gestation and the total implantaion, total life and death fetuses, as well as resorpted embryos, were taken as data. The results showed that vitrification significantly (p<0,05) reduced the quality of the embryos, as well as their implantation rate and the viability of the fetuses, which may be caused by the unoptimal combination of the cryoprotectant in the vitrification medium, temperature and exposure time during vitrification.
Arab Descendant Identity in Media: A Discourse Analysis of Suara al-Irsyad Madihah, Madihah; Sarbini, Ahmad; Muhyiddin, Asep; Wibisono, M. Yusuf
Hanifiya: Jurnal Studi Agama-Agama Vol. 8 No. 1 (2025): Hanifiya: Jurnal Studi Agama-Agama
Publisher : Program Studi Studi Agama-Agama Pascasarjana UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/hanifiya.v8i1.44702

Abstract

This study examines the representation of Arab descendants’ identity in Indonesia through the community-based media Suara Al-Irsyad (MSA). Media play a crucial role in shaping and preserving community identity, particularly within diasporic groups facing globalization and social transformation. This research aims to explore how MSA contributes to maintaining Arab descendants’ cultural identity and the strategies employed to remain relevant in the digital era. Employing a qualitative approach with a descriptive-analytical method, data were collected through direct observation, in-depth interviews with MSA administrators, and document analysis of its publications. Data analysis followed the Miles and Huberman model, encompassing data reduction, data display, and conclusion drawing. The findings indicate that MSA functions not only as an information platform but also as a medium for cultural preservation. Beyond reporting news and Islamic education, MSA constructs an inclusive narrative that highlights the contributions of Arab descendants to national development. To address digital challenges, MSA has implemented modernization strategies by leveraging social media platforms to engage younger audiences. Despite declining interest in community-based media and increasing competition from mainstream platforms, MSA sustains its relevance by balancing traditional values with technological innovation. This research contributes to media and identity studies by demonstrating the critical role of community media in constructing minority group representations in the public sphere. It enriches the discourse on diaspora identity dynamics in the context of media, particularly in navigating globalization and social change. Moreover, the study provides practical implications for media practitioners, scholars, and policymakers in formulating sustainable strategies for ethnic-based media in the digital era.
Learning Through "ARBOR Exhibition": Utilizing the Arboretum of Universitas Padjadjaran for Innovative Learning in Higher Education Rohmatullayaly, Eneng Nunuz; Irawan, Budi; Madihah, Madihah
Khizanah al-Hikmah : Jurnal Ilmu Perpustakaan, Informasi, dan Kearsipan Vol 13 No 1 (2025): June
Publisher : Program Studi Ilmu Perpustakaan UIN Alauddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/v13i1a15

Abstract

The Arboretum Universitas Padjadjaran, as a living collection or museum, is a valuable educational resource for science education, promoting hands-on activities, student-centered learning, and rich social interaction. Aligned with the Outcome-Based Education (OBE) curriculum, the arboretum can be integrated to develop learning innovation, particularly in the “Digitalization of Biological Objects,” which leads to bio-curators’ competencies. Visual arts can be an excellent strategy for learning science, as it combines systematic work with creative thinking. Through this approach, the students can apply theory to real-life experiences. This study aimed to utilize the Arboretum Universitas Padjadjaran to implement innovative strategies for effective learning in higher education through exhibitions. This study used the Research and Development (R&D) method. “ARBOR: The Art and Beauty of Universitas Padjadjaran’s Pollinator” is a photography talk show and exhibition providing information about pollinators, food plants, and host plants in the arboretum and surrounding campus. A total of 135 curated photographs were exhibited digitally. During the exhibition, the students guided over 250 visitors on pollinators and their role in the ecosystem. Our result shows that project-based learning, such as “ARBOR” exhibitions, increased awareness regarding biodiversity and environmental issues and enhanced the students' leadership, communication, creative thinking, innovation, and collaboration skills, demonstrating the inspiring potential projects in higher education.
PERILAKU MAKAN IKAN NILA (Oreochromis niloticus L.) DI KOLAM CIPARANJE UNIVERSITAS PADJADJARAN KAMPUS JATINANGOR Angelica, Irene Teresa; Madihah, Madihah
BIOTIKA Jurnal Ilmiah Biologi Vol 23, No 1 (2025): BIOTIKA JUNI 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v23i1.63387

Abstract

Ikan nila (Oreochromis niloticus L.) adalah ikan air tawar dengan prospek ekonomi tinggi. Analisis perilaku makan ikan dapat memberikan informasi mengenai komposisi pakan alami ikan yang bermanfaat untuk pengembangan protokol budidaya dan pelestarian populasinya. Perilaku makan ikan berkaitan dengan ketersedian jenis pakan di habitatnya. Penelitian ini bertujuan mengetahui perilaku makan ikan nila dari Kolam Ciparanje, Universitas Padjadjaran, Jatinangor berdasarkan hasil analisis frekuensi kejadian makanan dan rasio panjang relatif usus. Frekuensi kejadian makanan menyatakan jenis pakan dan kuantitasnya, rasio panjang relatif usus menyatakan golongan perilaku makan ikan berdasarkan anatomi usus. Metode penelitian ini adalah survei pada 15 ekor ikan nila berukuran 12,6-23,9 cm. Frekuensi kejadian makanan diamati dengan membandingkan jumlah lambung yang berisi suatu jenis pakan dengan total jumlah lambung yang diamati. Rasio panjang relatif usus ikan diukur dengan membandingkan rata-rata panjang total saluran pencernaan dengan panjang total tubuh ikan. Data dianalisis secara kuantitatif dan diinterpretasikan secara deskriptif. Hasil penelitian menunjukkan 21 jenis fitoplankton dan 1 jenis zooplankton pada lambung ikan nila. Fitoplankton yang mendominasi adalah Schroederia setigera pada 14 dari 15 lambung (93.33%), sementara zooplankton yang ditemukan hanya Filinia sp. pada 6 dari 15 lambung (40%). Nilai rasio panjang relatif saluran pencernaan adalah 5.36±. Hasil ini memperkuat penelitian lainnya yang menyatakan kebiasaan makan ikan nila adalah herbivora dengan preferensi pemakan plankton.
INFLUENCES OF INCUBATION TIME AND SUCROSE CONCENTRATION ON MICE (Mus musculus L.) OOCYTE VIABILITY FOR ENUCLEATING PROCEDURE Kaiin, Ekayanti Mulyawati; Gunawan, Muhammad; Madihah, Madihah; Nafisah, Ghina
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.10896

Abstract

This study aimed to find out the optimum incubation time to complete mouse oocyte maturation at Metaphase II (MII) stage and determine the optimum sucrose concentration enabling to induce nuclear swelling for visualization that is important for enucleating process at the initial procedure of somatic cell nuclear transfer (SCNT). In this current study, mice were used as animal model. Completely randomized design was arranged, consists of 2 trials with 4 treatments and 7 replications. In the first trial, the oocytes were cultured at 0-2, 4-6, 8-10, and 12-14 h in 5% CO2 incubator at 37 C. Second, the MII oocytes obtained from previous trial were cultured in M199 medium containing different concentrations of sucrose (0, 1.5, 3, and 6%). The parameters measured were the oocyte viability at various stages, i.e germinal vesicle (GV), metaphase I (MI), anaphase/telophase I (A/T I), and metaphase II (MII), and the viability of swollen nuclear oocytes using Hoechst/PI staining. The results showed that the optimum incubation time required by oocytes to reach MII stage was 12-14 h with a percentage of 57.1412.67%, while the optimum sucrose concentration for nuclear swelling was found at 3% with a percentage of 1000.00%. Our findings provided preliminary results related to the maturation process of the mouse oocyte nucleus, which is meaningful for the initial procedure of SCNT.
BACTERIAL CONTAMINATION TEST IN POWDER-FORMULATED Helicoverpa armige-ra NUCLEAR POLYHEDROSIS VIRUS (HaNPV1) SUBCULTURE Miranti, Mia; Kasmara, Hikmat; Fitriani, Nurullia; Melanie, Melanie; A'yun, Inas Qurrata; Syaputri, Yolani; Doni, Febri; Madihah, Madihah; Rahayuningsih, Sri Rejeki; Azizah, Nabilah Sekar; Hermawan, Wawan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2947

Abstract

The Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV1) is a subculture derived from the original HaNPV, and it has been cultivated in Spodoptera litura larvae as an alternative host. HaNPV1 was subsequently formulated using gypsum and talcum as carrier media. Following this formulation, a bacterial contamination test was conducted to assess the quality of the viral formulation.  The experiment was arranged in the randomized factorial block design (RFBD) with 2 replications. The viral formulations was stored for 16 weeks and the samples were taken every two weeks for contamination analysis. The data was then analyzed with the analysis of variance (ANOVA) and a post-hoc using Duncan’s Multiple Range test. The variable observed was the number of the bacterial colonies cultivated on the specific media i.e., Nutrient Agar (NA), Salmonella Shigella Agar (SSA) and Eosin Methilen Blue Agar (EMB). The results showed that the bacterial contaminants was detected from 0 to 12 weeks of storage time. However, the highest contamination was found in viral formulation after 8 weeks of storage time and the highest bacterial contaminations were recorded from all viral formulation tested in NA. The results indicated that the bacterial contamination were found around 1.45 × 109 cfu/gram and 1.97 × 109 cfu/gram in gypsum and talcum formulations, respectively. On SSA and EMB media, the bacteria contaminants from all formulation found in 8 weeks of storage time, but Salmonella, Shigella, or Escherichia coli (aspathogenic bacteria) were not found. After 12 weeks storage time, there was no indication of  contamination found in all media. Furthermore, Bacillus species was found as a most dominant contaminant in all samples. In conclusion, although the viral formulations using gypsum and talc were not contaminated by pathogenic bacteria such Salmonella, Shigella, or E. coli. Nevetherless, the viral formulation was still easily contaminated by other non-pathogenic bacterial species. Thus, a more standardized and stricted strategy needs to be developed for a better viral formulation product.
Co-Authors A'yun, Inas Qurrata Ahmad Sarbini, Ahmad Angelica, Irene Teresa Arief Boediono Asep Muhyiddin, Asep Azizah, Nabilah Sekar Budi Irawan Desak Made Malini Eneng Nunuz Rohmatullayaly Febri Doni Hikmat Kasmara Johan Iskandar Joko Kusmoro Kaiin, Ekayanti Mulyawati Kamilawati, Fitri Kartiawati Alipin Kusumaningtyas, Hartanti M. Yusuf Wibisono, M. Yusuf Malini, Desak M. Melanie Melanie, Melanie Mia Miranti Muhammad Gunawan Nafisah, Ghina Nurullia Fitriani Perdana, Andi Rochman, Indra A. Sri Rejeki Rahayuningsih Sumarsono, Sony H. Supartinah, Iin Syaputri, Yolani Wawan Hermawan