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Brigitta Laksmi Paramita
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,193 Documents
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Karakterisasi Kromosom Sel Heterohibrida Dengan Teknik Karyotiping I Ketut Berata
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2647

Abstract

The aim of the research was to characterize the chromosomal structure of the heterohybride cells by karyotyping technique. Heterohybride cells were made by fusion of Bali cattle lymphocyte cells with mouse myelomma in selective medium which contains hypoxanthin, aminopterin and thymidine. Those cells which showed growth then were identified, selected and isolated for characterizing its chromosomal structure by karyotyping technique. The result showed that all of the chromosomal structure of the heterohybride cells were diploidy. The average of diploidy chromosome of heterohybride cells were 97 diploid Bali cattle lymphocyte cells and myelomma of mouse. The conclusion of the research is the heterohybride cells do not contain abnormal chromosome and are most potent to develop as candidate of Jembrana diseases vaccine.
Effect of AMF Inoculation on the Growth of Combretocarpus rotundatus (Miq) on a Peat Soil from Central Kalimantan (For Restoration Ex-Mega Rice Project Central Kalimantan) Burhanuddin Burhanuddin; Siti Kabirun; Bostang Radjagukguk; Sumardi Sumardi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2649

Abstract

Diperlukan teknik silvikultur dengan memanfaatkan jamur mikoriza arbuskula (JMA) untuk memulihkan hutan rawa gambut yang terdegradasi. JMA berperan penting pada pertumbuhan dan ketahanan hidup pohon-pohonan. Penelitian ini bertujuan untuk mengetahui keberadaan dan bentuk dari JMA di hutan rawa gambut yang sudah terdegradasi pada blok C bekas pengembangan lahan gambut satu juta ha di Kelampangan Kalimantan Tengah. Kondisi lahan hanya ditumbuhi vegetasi jenis-jenis pioner. Contoh tanah diambil dibawah perakaran lima jenis tanaman pioner (Melastoma sp, Combretocarpus sp, Acacia sp, Cratoxylon sp, dan Nephrolepsis sp), kemudian contoh tanah dihitung jumlah spora dengan metode saringan basah. Jumlah spora terbanyak ada di bawah perakaran tanaman Melastoma sp, kemudian Acacia sp, dan Combretocarpus sp. Didapatkan tiga genus JMA: Glomus, Gigaspora, dan Acaulospora. Penelitian di rumah kaca bertujuan untuk mengetahui pengaruh inokulasi JMA jenis Glomus sp TD15, Glomus sp D32, dan Gigaspora sp pada semai perepat (C. rotundatus). Hasil penelitian menunjukkan JMA memberikan pengaruh pada parameter semai yang diukur. Jenis JMA Glomus sp TD15 meningkatkan pertumbuhan semai perepat yang ditunjukkan oleh parameter tinggi semai, diameter semai, dan jumlah daun per tanaman. Pada media gambut semai C. rotundatus sangat tergantung pada asosiasi JMA dalam meningkatkan pertumbuhan tanaman.
Pengaruh Pemberian Angkak Monascus purpureus terhadap Kualitas Mikrobiologis Feses Tikus Putih Hiperlipidemia T Yulinery; R. Hardiningsih; N. Nurhidayat
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2650

Abstract

Angkak is defined as rice fermented by Monascus purpureus, a lovastatin and pigmen producer. The objective of this research was to observe the effect of angkak powder to the digestion system represented by the quality of faeces. The in vivo bioassay was carried out using 20 Sprague-Dawley strain mice into 5 groups. First group was given angkak respectively 0.01g, 0.1g, 0.5g, and two groups of control (positive and negative). The faeces were examined each week for a month. The number or erytrosit, bacteria, and fungus were examined microscopically. The results showed that 0,01g, 0,1g, 0,5g of angkak did not significantly affect of bacteria, fungus and erythrocyte on faeces. The data suggest that the administration of angkak might be useful and safe for the hypercholestrolemic treatment.
Pemeliharaan Planaria Dalam Perkembangbiakan Secara Vegetatif Hertien Koosbandiah Surtikanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2651

Abstract

Test organism which is used for bioassay must fulfill some criteria such as: high sensitifity, widely available, wide distribution, biology background, successfully maintained in laboratory, and known history of culture in laboratory. This research has been done to study regeneration (vegetative) process of Planaria under laboratory condition. This is studied because of limited number of planaria population in clean freshwater. In order to study regeneration process, combination of two treatments (water conductivity and division-cutting type) were done to obtain optimum culture of Planaria. Four water conductivity (100, 200, 300 and 400 μS/cm) and four division-cutting types (whole longitudinal, half longitudinal, transversal above pharynx and transversal mid-pharinx) were used. Each individual of Planaria was exposed with those treatments for 10 days (October, 2002). Initial and completed growth were observed. The result showed that, regeneration process of Planaria took 3-6 days to get full growth after transversal cutting (mid-pharynx). Whole summary revelead that Planaria is easy to maintain in laboratory. Therefore, Planaria may be used as an alternative bioindicator in evaluating water pollution.
Sebaran dan Kondisi Karang Batu di Perairan Kecamatan Wori, Sulawesi Utara Jemmy Souhoka
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2652

Abstract

Hard or stony coral is one important component to structure the coral reef ecosystem and play a significant role for its marine life and its surrounding. Therefore, the condition of reef ecosystem needs to be maintained. Research on stony coral condition and distribution in waters of Wori Village, North Sulawesi, was conducted in April 2008. Line Intercept Transect was applied to assess the coral condition. A 50 m line transect was set on the 5 - 7 m depth horizontally coastal line with assumption that in this depth, coral growth well. The results of this study show that the condition of coral was between 1,00 and 53,44% indicating the condition ranged from bad to good category. Stony coral distribution was found up to 20 m depth, with the highest diversity and evennes indices were 1,06 at station 3, and 0,56 at station 4, respectively. Numbers of stony coral were 99 species belonging to 40 genus and 14 families.
Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Sub-unit 2 Mitokondria Rini Widayanti; Niken Satuti Handayani; I. Made Budiarsa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2653

Abstract

Tarsius is an endemic species in Indonesia that is endangered. In-situ and ex-situ conservation of this species would yield better results if this genetic make up and diversity is determined. The objective of this ressearch was to study the specific genetic marker on COX2 gene of Tarsius sp. Sequencing of PCR product using primer COX2F and COX2R resulted in base sequence of 513 nts. Results of COX2 fragments sequencing were put on multiple alignment with other primates from Genbank with aid of software Clustal W, and were analyzed using MEGA program version 4.1. Eight different amino acid sites were found (amino acid no. 5, 6, 13, 14, 30, 35, 44 and 168). The genetic distance based on nucleotide COX2 calculated using Kimura 2-parameter model indicated that in the smallest genetic distance 0%, biggest 6.8% and average 2.3%. The phylogenetic tree using neighbor joining method based on the sequence of nucleotide and amino acid COX2 reveded differentiation among Tarsius from Lampung and Tarsius from Sulawesi, but could not be used to differentiate among T. dianae (from Central Sulawesi) and T. spectrum (from North Sulawesi).
Pengaruh Pemberian Ekstrak Kulit Batang Ailanthus altissima terhadap Perubahan Sel Hati dan Ginjal Mencit Praptiwi Praptiwi; Emma Sri Kuncari; Dewi Ratih Agungpriyono; Chairunnisa Chairunnisa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2654

Abstract

The aim of this study was to determine the toxicopathological effect of Ailanthus altissima extract in liver and kidney of the mouse. The treatments in this study were: (1) negative control (carboxy methocellulose), extracts with concentration of (2) 1 mg/kg BW, (3) 10 mg/kg BW, (4) 100 mg/kg BW, and (5) 1000 mg/kg BW. Every treatment consisted of 5 mice. After receiving A.altissima extract for seven day consecutively, the mice were then euthanized with overdose of ether. The liver and kidney were taken and then fixed within 10% solution of Buffer Neutral Formalin (BNF). Histopathology slides of liver and kidney were processed and stained with Hematoxylline Eosin. The cell degeneration and cell death were observed with video photo microscope. The lesion of cell death and degeneration of hepatocytes and epithelium of kidney proximal tubule were counted. The data were analyzed statistically with ANOVA test continued with Duncan test. The result showed that cell necrosis of hepatocytes was not significantly different (P>0.05) compared to control treatment, while cell necrosis was increasing in the treatment of ≥ 10 mg/kg BW in tubuli of the kidney.
Kepadatan Populasi dan Sebaran Cacing Tanah di Lahan Sawah Sistem Pertanian Organik, Semi Organik dan Konvensinal Ea Kosman Anwar; RDM. Simanungkalit; Edi Santoso; Sukristiyonubowo Sukristiyonubowo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2655

Abstract

Earthworm is one of the soil macrofauna, which consumes organic matter for body growth and life. Therefore the earthworm can be soil organic status and soil fertility indicator. On the other hand, at present, the organic farming system is developing and positively accepted by people as yield consumer, and yield price is higher than conventional yield price. The experimen to study the earthworm population rate and spread methode was conducted in 2008 planting season, in West Java, with RBD (Randomized Block Design). Three farming system as treatment i.e Organic farming, Semi organic and Conventional farming system and six location as replication. The result showed that Semi organic farming system is the most earthworm population rate and the earthworm distribution is random model.
Uji Antimikroba Fraksi Ekstrak Metanol, Etil Asetat dan n-Heksana Daun Tabar-Tabar (Costus speciosus) dan Toksisitasnya Terhadap Larva Udang Dwi Suryanto; Tata B Kelana; Sri Wahyuni
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2656

Abstract

A Study on antimicrobial examination of fractions of methanol, ethyl-acetate, and n-hexane extract of tabar-tabar (Costus speciosus) leaf to bacteria (Bacillus sp., Staphylococcus aureus, Escherichia coli, and Serratia marcescens) and yeast (Candida albicans), and their toxicity to brine-shrimp (Artemia salina) larvae has been done. Examination of antimicrobial activities to the microbes were done by agar diffusion method, while test for toxicity was done by exposing 2 days-old larvae to certain concentration of the extract. Preliminary tests on phytochemical compounds in leaf were done by Mayer’s, FeCl3, Mg-HCl, foam, and 10% NaOH in methanol tests. The results showed that methanol leaf extract of tabar-tabar in general inhibited more in the growth of gram-positive Bacillus sp. and S. aureus rather than to other tested microbes. Compared to methanol and ethyl-acetate fraction, n-hexane fraction of the leaf inhibited more Bacillus sp. and E. coli. Interestingly, C. albicans was highly inhibited by n-hexane fractions. LC50 of methanol extract, ethyl-acetate, and n-hexane fraction, were 45.53, 478.37, and 824.20 ppm, respectively. Preliminary test on phytochemical compounds showed saponin was only detected in fractions of methanol and ethyl acetate extracts, steroid was detected in fraction of methanol, while triterpenoid was detected in fraction of n-hexane extract. Phenolic was detected in all extract fractions, while flavonoid, kumarin, and alkaloid on the other hand were not detected.
Analisis Keragaman Genetik Dyera costulata (Miq) Hook.f. Berdasarkan Marka “Random Amplified Polymorphic DNA” Yuyu Suryasari Poerba; Elizabeth A. Widjaya
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2657

Abstract

Dyera costulata (Miq.) Hook.f (Apocynaceae) is a large tree of the lowland tropical rain forest of Southeast Asia that occurs in Thailand, the Malay Peninsula and on the islands of Sumatera and Borneo. Its economic value was in its latex, used as gum chile in the manifacture of chewing gum. Nowadays the timber of this species is largely utilized for the manufacture of pencils and picture frames. The information on genetic diversity of the species is very limited. Hence studies were initiated and genetic diversity were estimated using Random Amplified Polymorphic (RAPD) markers in 47 accessions of Dyera costulata procured from different geographical regions of Jambi. Four selected Operon primers (10 mer) generated a total of 90 consistent amplification products ranging from 150 bp to 2.8 Kb. The cluster analysis showed that the 47 individuals were separated into one main cluster and one individual. The range of genetic dissimilarity value among samples was from 0.06 to 0.71, while genetic distance among populations was from 0.17 to 0.42. These values showed that those 47 accessions of D. costulata from Jambi was genetically originated from diverse population.

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