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Jurnal Ilmiah Farmasi
Published by Universitas Islam Indonesia
ISSN : 16938666     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
JIF merupakan jurnal yang dikelola oleh Prodi Farmasi Universitas Islam Indonesia, dan diterbitkan dua kali dalam setahun. Jurnal ini dirancang sebagai sarana publikasi penelitian yang mencakup secara rinci sejumlah topik dalam bidang farmasi yang berkaitan dengan farmasi sains dan teknologi serta klinik dan komunitas. Jurnal ini menyediakan sebuah forum sebagai sarana pertukaran gagasan dan dan informasi antar peneliti, akademisi dan praktisi sehingga diharapkan mampu mendukung dan menginisiasi berbagai penelitian terkini yang terkait dengan ilmu kefarmasian. Hasil penelitian yang disajikan dalam jurnal ini diharapkan dapat memberikan kontribusi bagi perkembangan ilmu di bidang farmasi dan kesehatan.
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Articles 269 Documents
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Uji inhibisi aktivitas enzim tirosinase beberapa jenis tumbuhan anggota suku Zingiberaceae Arini Aprilliani
Jurnal Ilmiah Farmasi Vol. 14 No. 1 (2018): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol14.iss1.art05

Abstract

Latar belakang dan tujuan: Paparan sinar UV secara terus menerus dalam jangka lama akan menyebabkan hiperpigmentasi pada kulit. Salah satu pencegahan hiperpigmentasi adalah dengan penghambatan aktivitas tirosinase. Beberapa suku Zingiberceae seperti kunyit, lengkuas dan bangle hantu dapat menghambat aktivitas tirosinase. Tujuan penelitian adalah untuk menguji inhibisi aktivitas enzim tirosinase dari rimpang beberapa jenis tumbuhan anggota suku Zingiberaceae. Selain itu, dilakukan pula terhadap hasil fraksinasi tumbuhan terpilih. Metode: Ekstraksi dengan cara maserasi menggunakan pelarut etanol 96 % dan fraksinasi menggunakan pelarut n-heksana dan etil asetat. Uji penghambatan tirosinase secara in vitro dengan kontrol positif menggunakan alpha-arbutin. Hasil: Hasil uji terhadap 19 sampel tumbuhan menunjukan adanya aktivitas inihibisi tirosinase. Ekstrak etanol rimpang kecombrang, kunci pepet, bangle hitam, temu giring dan jahe merah memberikan nilai inhibisi tertinggi terhadap aktivitas tirosinase dengan persen inhibisi masing-masing sebesar 22,50 ± 1,46 %, 20,75 ± 0,04 %, 19,96 ± 0,03 %, 18,85 ± 0,11 %, 18,63 ± 0,06 %. IC50 ekstrak rimpang kecombrang (761,75 ± 23,1 mg/L) lebih tinggi dibandingkan IC50 yang dihasilkan oleh fraksi n-heksana rimpang kecombrang (575,37 ± 4,1 mg/L), fraksi etil asetat rimpang kecombrang (542,39 ± 12,4 mg/L) dan fraksi air rimpang kecombrang (587,40 ± 2,6 mg/L). Sehingga dapat disimpulkan bahwa ketiga hasil fraksi memiliki IC50 lebih baik dibandingkan ekstrak. Fraksi etil asetat rimpang kecombrang memiliki penghambatan tirosinase lebih baik secara signifikan dibandingkan fraksi air rimpang kecombrang dan fraksi n-heksana rimpang kecombrang.Kata kunci: tirosinase, Zingiberaceae, alpha-arbutin, kecombrang. 
SISTEM PENGHANTARAN OBAT MELEWATI BARRIER DARAH OTAK Syukri, Yandi
Jurnal Ilmiah Farmasi Vol. 1 No. 1 (2004)
Publisher : Universitas Islam Indonesia

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Abstract

ABSTRACTBrain barrier is effective barrier in drug delivery to brain. For effectiveness drug deliveryneed be desaigned a delivery with nanoparticle technology. Nanoparticles are solid colloidalparticles ranging in size from 1 to 1000 nm that are utilized as drug delivery agents. The primaryadvantages of nanoparticle carrier technology is that nanoparticles mask the blood – brain barrierlimiting characteristics of the therapeutic drug molecule. Furthermore, this system may slow drugrelease in the brain, decreasing peripheral toxicity. The method which elaboration in manufacturenanoparticles are emulsion polimerization, interfacial polimerization, desolvation evaporation andsolvent deposition. Currently, report evaluating nanoparticles for brain delivery have studiedanesthetic and chemoterapeutic agent. These studies are reviewed for efficacy and mechanisms oftransport. Physiological factors such as phagocytic activity of the reticuloendothelial system andprotein opsonization may limit the amount of brain delivered drug. Nanoparticle technology appearsto have significant promise in delivering therapeutic molecules across the blood-brain barrier.Key Word : Drug Delivery System, Nanoparticle, Blood-Brain Barrier
Pola penggunaan furosemid dan perubahan elektrolit pasien gagal jantung di Rumah Sakit X Yogyakarta Mawaqit Makani; Ndaru Setyaningrum
Jurnal Ilmiah Farmasi Vol. 13 No. 2 (2017): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol13.iss2.art3

Abstract

IntisariLatar belakang: Gagal jantung merupakan tahap akhir dari seluruh penyakit jantung atau kelainan jantung yang berpengaruh terhadap perikardium, katup jantung, dan miokardium. Furosemid merupakan obat umum yang digunakan pada pasien gagal jantung. Efek diuretik furosemide dapat menyebabkan deplesi cairan dan elektrolit dalam tubuh.Tujuan: Tujuan dari penelitian adalah untuk mengetahui pola penggunaan furosemid dengan melihat kadar elektrolit pasien.Metode: Penelitian ini merupakan penelitian deskriptif dengan rancangan cross sectional. Pengumpulan data yang digunakan dalam penelitian ini adalah data sekunder yang diambil dari data rekam medik pasien di RS PKU Muhammadiyah Yogyakarta Periode 2011-2014 yang memenuhi kriteria inklusi.Hasil: Hasil penelitian ini menunjukkan bahwa furosemid intravena 3x1 (20mg/2ml) paling banyak digunakan pada 32 pasien (45%). Respon terapi furosemid dilihat dari kadar elektrolit yaitu; 7 pasien (17%) mengalami kejadian hiponatremia ringan, 10 pasien (25%) mengalami hipokalemia (4 pasien hipokalemia ringan, 6 pasien hipokalemia sedang). Pemberian furosemid intravena 20 mg / 2ml diberikan pada pasien yang mengalami edema. Sedangkan pemberian furosemid 40 mg oral diberikan pada pasien yang mengalami perbaikan kondisi setelah edema.Kesimpulan: Respon terapi furosemid berdasarkan tinjauan kadar elektrolit ditemukan 7 (17%) hiponatremia ringan, 4 (10%) hipokalemia ringan dan 6(15%) hipokalemia sedang.Kata kunci: gagal jantung, furosemid, respon terapi
EVALUASI TERAPI PADA PASIEN HEPATITIS B DI RSUP Dr. SARDJITO YOGYAKARTA Rahmannisa Wikan Trisnaningtyas; Chynthia Pradiftha Sari; Ndaru Setyaningrum
Jurnal Ilmiah Farmasi Vol. 13 No. 1 (2017): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

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Abstract

 Abstract Chronic viral hepatitis B infection (HBV) is a serious problem because its worldwide spread is quite high. To date, an estimated 350 million people are infected with HBV. This study aims to determine the profile, antiviral compatibility with the guidelines of the Indonesian Heart Research Association (PPHI) in hepatitis B inpatients at the Central General Hospital (Dr). Sardjito Yogyakarta. This was an observational study using cross sectional design. Data was collected from June to October 2015 on medical records of inpatients in 2012-2014 at Dr. Sardjito Yogyakarta. Number of respondents who meet the criteria of 91 patients. Data collected in the form of secondary data, including patient demographic data including age, sex, data treatment of patients (type of drug, dose, frequency and duration). The results are described descriptively and presented in table / percentage form. The hepatitis B patient's therapy profile shows the use of supportive therapy 26,88%, antiviral therapy 3,75%, other therapy 69,37%, antiviral therapy matching data given 100% according to guideline Keywords: Hepatitis B, profile therapy, apppropriatenes Intisari Infeksi kronik virus hepatitis B (HBV) merupakan masalah yang serius karena penyebarannya di seluruh dunia cukup tinggi. Sampai saat ini diperkirakan sekitar 350 juta orang terinfeksi HBV. Penelitian ini bertujuan untuk mengetahui profil, kesesuaian antiviral dengan guideline Perhimpunan Peneliti Hati Indonesia (PPHI) pada pasien hepatitis B rawat inap di Rumah Sakit Umum Pusat (RSUP) Dr. Sardjito Yogyakarta. Penelitian ini merupakan penelitian observasional dengan menggunakan rancangan cross sectional (potong lintang). Pengambilan data dilakukan bulan Juni-Oktober 2015 pada rekam medik pasien rawat inap tahun 2012-2014 di RSUP Dr. Sardjito Yogyakarta. Jumlah responden yang memenuhi kriteria sebanyak 91 pasien.. Data yang dikumpulkan berupa data skunder, meliputi data  demografi pasien meliputi umur, jenis kelamin, data pengobatan pasien (jenis obat, dosis, frekuensi dan durasi). Hasil penelitian digambarkan secara deskriptif dan disajikan dalam bentuk tabel/persentase. Profil terapi pasien hepatitis B menunjukkan penggunaan terapi suportif 26,88%, terapi antiviral 3,75%, terapi lain-lain 69,37%, data kesesuaian terapi antiviral yang diberikan 100% sesuai guideline. Kata kunci: Hepatitis B, , profil terapi, kesesuaian terapi 
Dizziness and nausea vomitting induced by ropinirole therapy in an elderly patient with Parkinson’s disease : a case report Emilia Sidharta; Hanny Cahyadi
Jurnal Ilmiah Farmasi Vol. 16 No. 1 (2020): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol16.iss1.art7

Abstract

Background: Ropinirole is a non-ergoline dopamine agonist drug that is widely used in a therapy for patients diagnosed with Parkinson’s disease. In long-term use, several published studies have mentioned the occurrence of side effects of ropinirole in the therapy of Parkinson’s disease, but there has been no case report on the occurrence of side effects in the form of dizziness and nausea-vomiting, especially in Indonesia. Case Presentation: This case study reported the occurrence of side effects in the form of dizziness and nausea-vomiting experienced by a 74-year-old elderly who was undergoing a treatment in a hospital in Indonesia. The patient was diagnosed with Parkinson's 8 months ago and has been given a combination therapy of levodopa-benserazide and trihexyphenidyl. During such period, no side effects occurred. The therapy was then supplemented with 2 mg ropinirole because the patient complained that his hand started shaking again. Some side effects arose after the addition of 2mg ropinirole; therefore, the side effects were thought to be associated with ropinirole. The assessment methods used were the time-series data collection followed by causality analysis using the Naranjo Scale. The analysis showed a score of 6, indicating Probable. Based on the literature review, side effects such as nausea and vomiting may occur due to the activation of dopamine D2 receptors in the Chemoreceptor Trigger Zone (CTZ) area. The CTZ area consists of several receptors, which are sensitive to the causative agent of emesis and produce information on the vomiting center that has a role in triggering the vomiting reflexes.Conclusion: Analysis using Naranjo Scale shows a score of 6 which indicates a probable association between dizziness, nausea-vomiting and ropinirole in an elderly patient with Parkinson’s Disease.Keywords: ropinirole, Parkinson, case report, elderly, side-effects
Physicochemical characterization of Sargassum polcystum C. Agardh and its activity against dinitrofluorobenzene-induced allergic contact dermatitis in mice Irish Mhel C. Mitra; Ross D. Vasquez; Reginald B. Salonga; Mary Jho-Anne Corpuz
Jurnal Ilmiah Farmasi Vol. 16 No. 1 (2020): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol16.iss1.art3

Abstract

Background: Sargassum polycystum C. Agardh is brown seaweed abundant in the Philippines. Recent studies showed that it has an anti-inflammatory property. However, its efficacy against allergic contact dermatitis (ACD) has not yet been studied and there are no established data regarding its physicochemical properties yet.Objectives: The objectives of this study were to evaluate the topical efficacy of S. polycystum crude polysaccharide (Spcp) using dinitrofluorobenzene (DNFB)-induced ACD murine model and to conduct physicochemical characterization on Spcp.Methods: ACD was induced by sensitizing the BALB/c mice through topical application of 0.5% DNFB on the shaved ventral skin. Spcp (25%, 12.5%, 6.25% w/w) and standard drug (Betamethasone 0.10%) were topically applied on the right ear of the mice for seven days after sensitization and right after the challenge on the eighth day. Seven days after sensitization, the right ear was challenged with 0.2% DNFB. Ear thickness was measured at baseline and 24-hrs post-challenge using a dial thickness gauge. Physicochemical characterization was also performed.Results: The results showed that topical application of Spcp inhibited the swelling produced during 24-hrs post-challenge. The analysis revealed that the 25% Spcp exhibited a statistically significant effect and was comparable with the inhibitory effect of the standard drug, betamethasone (p<0.05). The physicochemical characterization showed that Spcp contains a notable amount of carbohydrates, sulfate, and protein.Conclusion: In conclusion, our results suggest that topically applied Spcp can be an effective natural product to treat allergic contact dermatitis. However, further investigations are required to understand the mechanisms involved.Keywords: allergic contact dermatitis, physicochemical, polysaccharide
Analysis of the level of knowledge of mothers about self-medication to children in Cangkringan District, Yogyakarta Yosi Febrianti; Dessy Melanita; Bondan Ardiningtyas
Jurnal Ilmiah Farmasi Vol. 16 No. 1 (2020): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol16.iss1.art8

Abstract

AbstractBackground: Self-medication refers to an endeavor that is mostly frequently done by society in coping with any symptoms of disease prior to have an aid from medical practitioner. In this case, knowledge about medication and any disease complaints will bring about the impact on the medication use. Insufficiency of mother in understanding about drug and the way of using it in self-medication is potential to be a factor of medication error both for the mothers themselves and for their family. Knowledge required to properly do self-medication is by identifying the active substances, indication, contraindication, dosage and side effect of the medication.Objective: This research is designed to observed the description of the implementation of self-medication, the description of knowledge level of mothers about self-medication and factors determining the knowledge level of mothers. Method: In addition, this research used questionnaires written in accordance with the Guidelines of Free Medicine Use and Limited Free Medicine. Categorization of the knowledge level of mothers is based on the final score of the questionnaires.Results: The result then showed that the knowledge level of the mothers about the general knowledge of medicine was at 61% for those categorized into good knowledge and 39% for those categorized into medium-level knowledge. Meanwhile, in terms of knowledge level of mother about complaint and diseases treatable using self-medication was at 90% for those categorized into good knowledge and 10% for those categorized at medium-level knowledge.Conclusion: The factors determining the knowledge level of mothers included age, educational level and income. On the other hand, the factor that mostly determined the knowledge level of mother was educational level.Keywords: self-medication, knowledge level, YogyakartaIntisari Latar belakang: Swamedikasi adalah upaya yang paling banyak dilakukan masyarakat untuk mengatasi gejala penyakit sebelum mencari pertolongan dari tenaga kesehatan Pengetahuan tentang obat dan keluhan penyakit berdampak pada penggunaan obat. Keterbatasan pengetahuan para ibu akan obat dan cara penggunaannya dalam swamedikasi dapat menjadi sumber terjadinya kesalahan pengobatan (medication error) pada diri sendiri dan anggota keluarganya. Pengetahuan yang dibutuhkan untuk melakukan swamedikasi dengan benar adalah mengetahui bahan aktif, indikasi, kontraindikasi, dosis, dan efek samping pengobatan. Tujuan: Tujuan penelitian untuk mengetahui gambaran pelaksanaan swamedikasi,mengetahui gambaran tingkat pengetahuan para ibu tentang swamedikasi dan mengetahui faktor- faktor yang mempengaruhi tingkat pengetahuan para ibu. Metode: Penelitian menggunakan kuesioner yang disusun berdasarakan Pedoman Penggunaan Obat Bebas dan Obat Bebas Tebatas. Pembagian golongan tingkat pengetahuan para ibu berdasarkan skor akhir kuesioner. Hasil: Tingkat pengetahuan para ibu tentang informasi umum obat, sebanyak 61% ibu tergolong pengetahuan baik dan 39% ibu tergolong pengetahuan sedang. Tingkat pengetahuan para ibu tentang keluhan dan penyakit yang dapat diatasi dengan swamedikasi, sebanyak 90% ibu tergolong pengetahuan baik dan 10% ibu tergolong pengetahuan sedang.Kesimpulan: Faktor yang mempengaruhi tingkat pengetahuan ibu antara lain usia, tingkat pendidikan dan tingkat penghasilan. Sedangkan faktor yang paling mempengaruhi tingkat pengetahuan para ibu adalah tingkat pendidikan.Kata kunci : Swamedikasi, tingkat pengetahuan, para ibu, Cangkringan 
Antidiabetic evaluation of Artocarpus odoratissimus (Moraceae) fruit Kay Ann S. Jonatas; Joseph Mari B. Querequincia; Shiela D. Miranda; Ukoba Obatavwe; Mary Jho-Anne Corpuz; Ross D. Vasquez
Jurnal Ilmiah Farmasi Vol. 16 No. 1 (2020): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol16.iss1.art1

Abstract

Background: Diabetes mellitus causes 4.2 million of deaths worldwide and 79% adults with diabetes are living in low- and middle-income countries. This research providing an alternative therapy through the prevention of postprandial hyperglycemia may help diabetic patients and provide a new utilization model of fruit peel. Artocarpus odoratissimus, commonly known as marang, is an edible fruit found in the southern part of the Philippines. Most of the weight of the fruit is discarded and treated as waste.Objectives: This study aimed to utilize the by-products of marang fruit as a promising pharmaceutical agent by determining the phytochemicals present and in vitro antidiabetic activity of the different parts of the fruit.Methods: Phytochemical screening of phenolics and flavonoids was done through thin layer chromatography. Ten concentrations (2-1000 µg/mL) of the extracts from the peel, pulp, and seeds were evaluated for the in vitro antidiabetic assay using alpha-glucosidase enzyme. Mean percent inhibition was calculated, and data was analyzed using ANOVA. The IC50 estimates were calculated using the program GraphPad Prism version 8.Results: Extracts from the fruit parts of A. odoratissimus contained phenols and flavonoids and were active inhibitors of alpha-glucosidase enzyme. The fruit peel extract of marang was the most potent (IC50 = 48.19 µg/mL) compared to the seed extract, pulp extract, and the standard drug acarbose (p value = 0.035).Conclusion: The fruit waste, the peel and seeds, has an intense activity against alpha-glucosidase enzyme because of their phenols and flavonoid contents.Keywords: alpha-glucosidase, Artocarpus, diabetes, phenolics, fruit peel
Fungal endophytes as the source of medicinal natural product Asih Triastuti
Jurnal Ilmiah Farmasi Vol. 16 No. 1 (2020): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol16.iss1.art6

Abstract

AbstractMassive exploration of medicinal plants as a source of medicinal raw materials and high demand for traditional medicines on the market has been a threat to biodiversity and plant species. To respond to the challenge of more efficient access to chemical diversity in a sustainable way, researchers have begun to focus their research on renewable sources, under-explored, but that have the prospect as the reservoir of new structures of bioactive metabolites, namely fungal endophytes. Fungal endophytes grow within the internal tissue s of the plant, without causing pathogenic symptoms have succeeded in producing secondary metabolites with diverse chemical structures and pharmacological activities such as antibacterial, antifungal, insecticide, antioxidant, anti hyperlipidemia, cytotoxic and anticancer. However, under conventional laboratory conditions, a plethora of secondary metabolites encoded in fungal endophytes were not produced presumably because the genes responsible for the secondary metabolites biosynthetic are not transcribed (remain silent). Several methods have been explored to activate these silent genes, including optimization parameters of fermentation, co-culture techniques, precursors/ plant extracts feeding, the addition of epigenetic modifiers such as DNA methyltransferase (DNMT) or histone deacetylase (HDAC) inhibitors, and genetic manipulation of biosynthetic and regulatory genes. The approaches in culture techniques are expected to bridge the debate in drug discovery and natural material production from endophytic fungi.Intisari Eksplorasi besar-besaran tanaman obat sebagai sumber bahan baku obat dan tingginya permintaan akan obat tradisional di pasaran telah menimbulkan permasalahan dalam biodiversitas dan ancaman bagi spesies tanaman. Peneliti bahan alam telah mulai memfokuskan penelitannya pada sumber terbarukan yang belum tereksplorasi namun memiliki prospek sebagai penyedia keanekaragaman struktur kimia, yaitu jamur endofit. Jamur endofit hidup di dalam jaringan tanaman tanpa menimbulkan simptom patogenik dan telah dilaporkan menghasilkan metabolit sekunder dengan struktur kimia yang beragam dengan aktivitas farmakologi yang luas seperti antibakteri, antijamur, insektisida, antioksidan, antihiperlipidemia, sitotoksik dan antikanker yang sangat potensial untuk dikembangkan dalam industri farmasi. Dalam pengembangannya, pemanfaatan jamur endofit memiliki beberapa kendala, utamanya dalam teknik kultur / fermentasi dalam rangka mengaktifkan gen penyandi biosintesis metabolit sekunder yang relatif inaktif selama kultur. Metode untuk mengaktifkan gen diam (silent gene) dapat dilakukan dengan beberapa cara yaitu: optimasi parameter fermentasi, teknik ko-kultur, penambahan prekursor atau zat antara ke dalam media kultur, penambahan modifikator epigenetik seperti inhibitor DNA methyltransferase (DNMT) dan atau inhibitor histone deacetylase (HDAC), dan manipulasi genetik. Pendekatan dalam teknik kultur jamur endofit diharapkan dapat menjembatani permasalahan dalam penemuan obat dan produksi bahan alam dari jamur endofit. Kata kunci: Jamur endofit, Aktivitas farmakologi, Aktivasi gen diam 
Evaluation of the hepatoprotective effect of methanolic extract of Caulerpa lentillifera against acetaminophen-induced liver toxicity in juvenile zebrafish (Danio rerio) Kimberly D. Codorniz; Rose Emielle M. Marquina; Alexandra Dominique G. Nolasco; Paula Denise D. Palencia; Sigfredo B. Mata
Jurnal Ilmiah Farmasi Vol. 16 No. 1 (2020): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol16.iss1.art4

Abstract

AbstractBackground: Liver injury is a common reason for drugs to be withdrawn from the market. Treatment options for common liver disease are limited, and therapy with modern medicines may lack effectiveness. Caulerpa lentillifera may have strong antioxidant systems that protect the plant from oxidative damage caused by the environment.Objectives: The main objective of this study was to evaluate the hepatoprotective effect of the methanolic extract of C. lentillifera against acetaminophen-induced liver toxicity in juvenile zebrafish (Danio rerio). Methods: Juvenile zebrafish (aged 1–3 months) were exposed to 10 mikromolar and 25 mikromolar acetaminophen (N-acetyl-p-aminophenol; APAP) to induce liver damage. C. lentillifera methanolic extracts (0.01 mg/L, 0.02 mg/L and 0.03 mg/L), were concomitantly added to individual tanks containing 0.01 M or 0.025 M APAP. The positive control group was treated with N-acetylcysteine/NAC (0.01 M) and silymarin (0.01 mg/L, 0.02 mg/L and 0.03 mg/L). Hematoxylin and Eosin (H&E) staining revealed the extent of liver injury through the presence of hepatic necrosis, vacuolization, leukocyte infiltration, and ballooning. The antioxidant mechanism of hepatoprotective activity was assessed by a DPPH free radical scavenging assay.Result: C. lentillifera extracts reduced the mortality of juvenile zebrafish when simultaneously exposed to APAP. Upon histopathological examination of the liver tissue of juvenile zebrafish, the group treated with the 0.01 M APAP together with the highest concentration of C. lentillifera extract showed minimal liver injury compared to the groups exposed 0.025 M APAP. However, the DPPH free radical scavenging assay performed using 24–36 mg/mL C. lentillifera extracts showed a minimal effect on the free radical scavenging activity.Conclusion: The histopathological analysis of the liver showed that C. lentillifera extract prevented the progression of liver damage caused by APAP. The results of DPPH free radical scavenging assay indicated that the hepatoprotective activity of C. lentillifera extract might have other antioxidant mechanisms aside from free radical scavenging. In order to effectively assess the improvement in the survival rate of juvenile zebrafish, longer exposure in the treatments is recommendedKeywords: C. lentillifera; juvenile zebrafish; hepatoprotective; drug-induced liver injury (DILI) 

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