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Isna Rasdianah Aziz
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Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
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Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 10 Documents
 1 
Search results for , issue "Vol 5 No 1 (2017)" : 10 Documents clear
Identifikasi Bakteri Salmonella sp. Pada Telur yang dijual di Pasar Kota Kendari Provinsi Sulawesi Tenggara Satya Darmayani; Anita Rosanty; Vina Vanduwinata
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3429

Abstract

This study aimed to identify the presence of Salmonella sp. in eggs sold in the market of Kendari. The World Health Organization (WHO) declared Salmonella sp. is a genus of bacteria that is a major cause of food borne illness in the entire world. Two factors that cause Salmonella sp. contained in eggs are internal and external factors that cause diarrhea or gastroenteritis. The sampling technique was done by purposive sampling, the population is the seller of eggs located in the market of Kendari city that is as much as 172 egg sellers with the sample size are 34 egg samples of chicken. In the process of planting samples on Brain Heart Infusion Broth (BHIB) media, all positive samples were cloudy, indicating bacterial growth in the sample. Furthermore, bacteria were inoculated in Salmonella Shigella Agar (SSA) medium which obtained negative results from 34 samples, no Salmonella sp. colony growth on SSA medium. So it can be concluded, the sample of chicken eggs in this study declared not contaminated by Salmonella sp.
Preferensi Pemijahan dan Habitat Ikan Nomei (Harpodon nehereus) di Perairan Juata Laut Tarakan Sebagai Upaya Konservasi Endik Deni Nugroho; Vlorensius Vlorensius; Adelyn Salurapa
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3434

Abstract

Nomei fish (Harpodon nehereus) is a local marine fish found in the territorial waters of Tarakan. This fish is a commercial fish that is widely marketed in dry form, so the fish Nomei become one of the specialties foods of Tarakan. Continuous Nomei fishing will have an impact on the fishermen's incomes that begin to fall and the extinction of these fish in ecosystem habitats. One effort that can be done to preserve the existence of Nomei fish is the basic research related to habitat and Nomei spawning preference that founded in coastal areas of Tarakan, North Kalimantan. The benefits of this research are as a reference in the determination of Nomei spawning habitat based on breeding preferences of Nomei, also as the basis of management and conservation of Nomei declining as well as efforts to conserve local marine fish of Tarakan. This research type is descriptive qualitative, with survey approach. Descriptive qualitative, explaining Nomei spawning preferences based on fish habitat types. The use of descriptive type is expected to determine the spawning preference and the dispersal habitat type of Nomei. Data collection is also inseparable from the source (fishermen) through interviews.
Ektrak Daging Buah Mahkota Dewa Meregenerasi Sel Pulau Langerhans Pada Tikus Putih Diabetes Fitranto Arjadi; Mustofa Mustofa
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3430

Abstract

In Indonesia, the increasing prevalence of diabetes mellitus is accompanied by the increasing financial burden to the patients. It is necessary to find alternative herbal treatment for diabetes, which is effective, cheap, and easy to find: the mahkota dewa. This research aims to find out the histological regeneration of Langerhans islet cells after given by flesh extract of mahkota dewa.This experimental research used complete randomized design (CRD). There were 5 treatments given to the twenty five mice; the negative control group (the normal one), the positive control (male white mice (Rattus norvegicus which were made diabetic by administering injection of 70 mg/kg of body mass of diabetogenic alloxan), the group of diabetic male white mice given 3, 6 and 9 grams/day/200 bodyweights), then were measured glucose in blood at 0, 1st and 4th week and counted Langerhans islet cells every 5 overviews.Statistically results showed that flesh extracts of mahkota dewa can decreased glucose in diabetic mice (r=0,75) with contribute 56,89% in decreasing, regenerated Langerhans islet cells of pankreas {Fcount(17,558)>Ftab(2,870)} with the highest increasing is 3,84 amount and started with 3grams/day/200 bodyweights doses, and the best effectiveness in regenerating was 11,81% consentration or 0,1181grams/day (6.56 grams/day in human).
Induksi Umbi Mikro Kentang Secara In Vitro Pada Suhu Tinggi Dengan Beberapa Tuber Promoter Usman Kris Joko Suharjo; Bambang Gonggo Murcitro; Tunjung Pamekas; Haryuni Haryuni
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3435

Abstract

Potato microtubers are the miniature of conventional seed tubers grown in the field. Producing potato microtuber in vitro at high temperature by applying many kinds of tuber promoting substances is similar to producing potato in the field at low elevation by applying the same tuber promoting substances. The objective of this research was to evaluate the performance of seven tuber promoters in inducing potato microtubers at high temperatures. Micro cuttings (6 weeks old) were grown at the liquid media of MS for stolon induction. Six weeks afterward, the media were moved to microtuber inducing media supplemented with tuber promoters ((FD, FD+10 COU, FD+20 COU, FD+30 COU, FD+40 COU, FD+50 COU, and IPB). The media were incubated in the dark at 20 + 2oC and 29 + 2oC. The results showed that, in general, high temperature inhibited crop growth and tuber formation. Individually, tuber promoter formula significantly affected microtuber emergence, in which IPB showed the slowest treatment in producing microtubers, the smallest number of tuber produced, and the smallest size of tubers. The interaction between temperature and tuber promoters were significantly affected the tuber formation, in which the fastest tuber formation (7,0 days ) was found in FD + 40 COU and the highest  percentage of plant producing tubers was found in FD + 30 COU. Moreover, the highest number of microtuber and the heaviest weight of microtuber were found in FD (no Coumarin). It was safely concluded that all tuber promoters were best performed at low temperature. Should anyone wish to make those tuber promoters work better at high temperature, it is recommended to increase the concentration of Coumarin added to the formula of tuber promoting substances.
Pengaruh Suhu Dan Intensitas Cahaya Terhadap Pertumbuhan Jamur Tiram Di Tangerang Fenny Amelia; Jose Ferdinand; Klerenita Maria; Michel Geren Waluyan; Indah Juwita Sari
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3426

Abstract

Oyster mushroom is a mushroom that can be consumed, because it contains many nutrients such as vitamins, minerals, and amino acid. Nowadays, demand for oyster mushroom in Indonesia continues to increase, especially in the lowland like Tangerang. Knowing this, it needs effort to control the habitat that oyster mushrooms can be cultivated. This research was conducted by giving three treatments where the first treatment directly using sun light, the second treatment using a lamp with 5 watts power, and a third treatment using a lamp with 15 watts power. The data obtained are progress of baglog and temperature measurements in each treatment and then the data were analyzed using ANOVA test. With 5% significance level, research results between treatments using direct sunlight with the light from the lamp gives a significantly different effect, while between a lamp with 5 watt power and a lamp with 15 watt power do not give a significantly different effect. Best oyster mushroom growth obtained at treatment using a lamp with 5 watts power.
Uji Daya Hambat Filtrat Zat Metabolit Lactobacillus plantarum Terhadap Pertumbuhan Shigella dysenteriae Secara In Vitro Rizki Putri Andini Rahmah; Meiskha Bahar; Yanti Harjono
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3431

Abstract

Shigella dysenteriae is pathogenic bacteria causes dysentery diarrhea, a diarrhea with mucus and blood in the feces. Currently, drugs or foods containing Lactobacillus have been circulated in the markets and used by people. This research aims to know the in vitro inhibition test of L. plantarum metabolite substances filtrate against growth of S. dysenteriae. This research used experimental design with L. plantarum metabolite substances filtrate as the samples. Antibacterial test method which was used was the cup-plate technique by looking at clear zone of bacteria’s growth around cup-plate and used Mueller Hinton Agar media. The results showed clear zones on filtrate with concentrations 25%, 50%, 75%, and 100% with average inhibition zones 0,284 cm; 0,332 cm; 0,406 cm; and 0,619 cm. Kruskal-Wallis test results indicated there were significant differences of each filtrate concentration against S. dysenteriae (p<0,05). Post Hoc test showed no significant difference between concentrations 25% and 50% (p>0,05). This suggested lactic acid, acetic acid, and bacteriosin presented in L. plantarum metabolite have antibacterial characteristic
Pengaruh Kadar Ampas Tahu Pada Pakan Ikan Gabus Channa gachua Terhadap Kadar Albumin Daging Jefri Permadi; Dhuta Sukmarani; Mudiyono Mudiyono
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3427

Abstract

The need for medical albumin is currently at 91%, which is relatively expensive price of albumin causes many people to switch to a cheap source of albumin of snakehead fish. This led to the exploitation of snakehead fish on a large scale so that the fish populations in natural will experience deterioration. Until now, the snakehead fish has not been cultivated by farmers because the fish is not a commodity featured and high production costs of the feed sector. Meanwhile, the potential for additional material feed nutrients are available quite easily and cheaply from the waste tofu. Utilization of bagasse as a mixture of snakehead fish feed can be an alternative to reduce the cost of the feed without reducing the quality of fish in terms of content snakehead fish albumin. In addition, tofu is used as dietary supplement of snakehead fish is expected to reduce the rate of environmental pollution caused by waste agricultural products. The method was used in this research is experimental. Snakehead fish is reared for 17 days with one control and four treatments of feed that is, commercial feed with a mixture of 20%, 40%, 50% and 70% tofu waste. After 17 days of snakehead fish from each treatment were analyzed its albumin levels. The data analysis using regression and correlation to determine the relationship between the treatments of feed against the observed variables. Results of regression and correlation analysis shows that administration of tofu waste in different concentrations has no effect on the feed exhibited significantly to weight and body length of the fish, the oxygen content, pH, water temperature, weight of the test sample and albumin levels in fish flesh. But the determinant of constant value shows that there is one variable that have relevance to the treatment. Based on Pearson correlation test, it is known that high levels of tofu waste given to the feed associated with increased levels of albumin catfish at each treatment. The highest levels of snakehead fish albumin is 4,835 g/100 g of fish meat on the treatment of 70% tofu waste out on feed, while the lowest levels of albumin is contained in the 2286 control treatment that is 0% tofu waste to the snakehead feed.
Sintesis dan Aplikasi Nanopartikel Kitosan Sebagai Adsorben Cd dan Antibakteri Koliform Swara Yudhasasmita; Andhika Puspito Nugroho
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3432

Abstract

Chitosan is a derivative compound of chitin which has a linear polysaccharide composed of β-(1–4)-linked d-glucosamine and N-acetyl-d-glucosamine. This compound is found in shrimp shell. In nanoparticle form, chitosan has a great antibacterial activity and  adsorption ability rather than normal form. The aims of this study are to study the effect of concentration chitosan nanoparticle to adsorpt cadmium and its antibacterial effect on coliform. This research was started with synthesis of chitosan nanoparticles using acetic acid 2%  and TPP 0,1 %, then the sampel was dried by spray dryer. For cadmium adsorption test was conducted by giving chitosan nanoparticle of 0.1, 0.2, 0.3, 0.4, and 0.5 g, respectively, into  Cd solution with the concentration of 7 ppm and was filtered with theWhatman paper number 42 (n=3). Cadmium contents in the filtrates and pellets were analyzed by using AAS. For antibacterial test with Salmonella typhimurium andEscherichia coliwere conducted by disc diffusion method, contained of 0.1, 0.2, 0.3, 0.4, and 0.5 g, respectively, were dissolved in 50 ml of 1% acetic acid. Data were analyzed by using oneway ANOVA followed by LSD. Based on the results, the optimum adsorption of cadmium present in addition of 0.4 g/ 50mL, can reduce Cd concentration by 98,7%. For the antibacterial test, the addition of 0.4 g/50mL is the largest zone of inhibition Salmonella thypimuriumand  Escherichia coli.
Kajian Kerentanan Beras Dari Padi Gogo Lokal Jambi Terhadap Sitophilus oryzae L. (Coleoptera: Curculionidae) Siti Fanda Rini; Hendrival Hendrival
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3428

Abstract

The research was aimed to determine the susceptibility level rice from the local gogo rice of Subdistrict Tengah Ilir, District Tebo, and Province Jambi to an infestation of S. oryzae pest. The results showed that rice from varieties Tuo and Seribu Naik belong to a category moderate, while rice from varieties Tapang Bedaro and Cemplok belong to a category moderate-susceptible. Rice from varieties Kuku Balam, Rias Besar, Rias Kecil, Korok, Bedaro, Nartih, and Bungin are classified as susceptible to S. oryzae pest infestations.  The result of correlation analysis showed that there was a significant positive correlation between susceptibility index with length rice (r= 0.906**, P<0.01) and shape rice (r= 0.771**, P <0.01) and there was a significant negative correlation with the wide of rice (r= -0.630*, P <0.05).  The susceptibility index was also affected by the number of F1 S. oryzae (r= 0.922**, P <0.01) and a negative correlation was significant with the median development time S. oryzae (r= -0.912**, P<0.01). Dimensions rice such as length rice (r= 0.898**, P<0.01), shape rice (r= 0.911**, P<0.01), and wide rice (r= -0.816 **, P<0.01) also affects the number of F1 S. oryzae of emerging. The dimension of rice is a source of susceptibility to rice against S. oryzae pests.
Derajat Ploidi Jahe Merah (Zingiber officinale Roxb. var. rubrum Rosc.) Hasil Induksi Dengan Kolkisin Meiliana Friska; Budi Setiadi Daryono
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3433

Abstract

Red ginger (Zingiber officinale Roxb. var. rubrum Rosc.) is one of the herb plants that often being used as a spice, traditional medicines, sweets, drinks, and material export commodities. The ginger’s rhizome is the most common because it is good for health freshness, and spice for food. The spicy from the ginger comes from ketone compounds ‘gingerol’. This research aims to produce polyploidy crops in red ginger by observing the changes of phenotype characters on the leaves, stems and rhizome resulted from colchicine induction. This research was based on experimental method with Randomized design with two factors, which were colchicine concentration (0,05%, 0,1% and 0,2%) and incubation time (6, 12 and 24 hours). Ploidy level was observed using flow cytometry technique. The data were analyzed using Analysis of Variance (ANOVA) and statistical data processing was done using F test at α = 5%. If the result shows any significant differences then was followed with Duncan Multiple Range Test (DMRT). The results showed that the change in the ploidy degree of red ginger was seen in K1W2 (0,05 % concentration of incubation time 12 hours) with increasing in ploidy from the diploid (2n) to mixoploid (2n= 2x+4x).

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