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Jurnal Penelitian Tanaman Industri
Published by Kementerian Pertanian
ISSN : 08538212     EISSN : 25286870     DOI : -
Core Subject : Engineering,
Industrial & Manufacturing Engineering
Jurnal Penelitian Tanaman Industri merupakan publikasi ilmiah primer yang memuat hasil penelitian primer komoditas perkebunan yang belum dimuat pada media apapun, diterbitkan oleh Pusat Penelitian dan Pengembangan Perkebunan, DIPA 2011 terbit empat kali setahun.
Arjuna Subject : -
Articles 552 Documents
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STABILITAS HASIL BEBERAPA GALUR WIJEN . SUPRIJONO; RUSIM MARDJONO; HADI SUDARMO
Jurnal Penelitian Tanaman Industri Vol 10, No 4 (2004): Desember, 2004
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v10n4.2004.127-130

Abstract

Penelitian dilaksanakan pada musim tanam 2003 di 4 lokasi yaitu, Kabupaten Lumajang, Bojonegoro, Nganjuk dan Sragen. Penelitian ini betujuan untuk memperoleh galur-galur unggul yang dapat beradaptasi dengan lingkungan pengembangan wijen dengan produktivitas tinggi. Rancangan yang digunakan adalah rancangan acak kelompok dengan tiga ulangan. Perlakuan tcrdiri dari 12 galur harapan yaitu SI.14, SI.16, SI.18, SI 20, SI.21, SI.24, SI.25, SI.26, S1.28, SI.31, SI.13, SI.40, dan sebagai pembanding digunakan 2 varietas komersial yaitu Sumberrejo 1 dan Sumberrejo 2. Hasil penelitian menunjukkan bahwa terdapat 4 galur unggul yaitu galur SI.14, SI.16, SI.18, SI.24 potensi hasil sama dengan varietas Sbr. I mempunyai adaptasi luas (galur stabil). Empat galur lainnya dapat bcrproduksi tinggi apabila ditanam pada kondisi lingkungan sesuai (spesifik lokasi). Galur SI. 21 dan SI 25 sesuai untuk daerah Nganjuk dan Sragen, galur SI 20 untuk daerah Bojonegoro dan Nganjuk, dan SI 28 untuk daerah Lumajang.Kata kunci : Wijen, Sesamum indicum L., galur, hasil, spesifik lokasi ABSTRACT Yield Stabibity of Sesame LinesThe experiment was conducted at four locations of sesame development area, viz. Lumajang, Bojonegoro, Nganjuk and Sragen districts, in 2003 planting seasons. The aim of this experiment was to find out the sesame lines having high productictivity and suitable for the development areas. Twelve promising lines, namely SI.14, SI.16, SI.18, SI.20, SI.21, SI.24, SI.25, SI.26, SI.28, SI.3I, SI.13, SI.40, and two control varieties (Sumberrejo 1 and Sumbenejo 2) were evaluated in randomized block design with three replications. The result of this experimental found out 4 superior lines (SI 14, SI 16, SI 18, and SI 24) that have the same potential as Sbr I variety, gave broad adaption to all locations (stable lines). Nevertheless the other four lines evaluated showed as the specific location lines. Those lines arc SI 21 and SI 25 that are appropriate for nganjuk and Sragen; SI 20 that is appropriate for Bojonegoro and Nganjuk, and SI 28 that is appropriate for Lumajang.Key words : Sesame, Sesamum indicum L., line, yield, specific location
TANGGAP BEBERAPA KLON NILAM (Pogostemon cablin Benth.) TERHADAP NEMATODA PELUBANG AKAR (Radopholus simitis Cobb.) YANG NURYANI; CHEPPY SYUKUR; RITA HARNI; YELNI TITIS; REPIANYO REPIANYO; IKA MUSTIKA
Jurnal Penelitian Tanaman Industri Vol 5, No 3 (1999): Desember, 1999
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n3.1999.103-108

Abstract

Response of some patchouli clones (Pogostemon cablin Benth.) to burrowing nematode (Radopholus similis Cobb.)One of the seious poblems on patchouli is infection of plant parasitic nematode. Nine clones of Aceh patchouli (Pogostemon cablin Benth.) namely KJ (oiginated from tissue cultue) Meulaboh-2, Sidikalang, Lhokseumawe-2, Cisaroni . Cirateun, Tapak Tuan. Aceh Merah. Situak-2, and one clone of Java patchouli (P. heyneanus Benth.) namely Giilaya, were tested to infection of buowing nematode (Radopholus similis Cobb.) in the glasshouse of Research Institute for Spice and Medicinal Crops, Bogor fom July to November 1997. Each clone was inoculated with 500 of R. similis. An expeiment have been conducted to know the esponse of some patchouli cultivars against nematode. The expeiment was arranged in a Completely Randomized Design with thee replicates. The esults showed that the infection of R. similis reduced root weight greater than that of shoot weight, the colour of leaf became reddish or dark purple. Based on the development rate of nematode and level of damage, Giilaya appeared to be resistant, whereas Sidikalang, Cisaroni and Situak-2 clones wee slightly susceptible. KJ. Lhokseumawe-2, Cirateun. Tapak Tuan and Aceh Merah clones were susceptible, whereas Meulaboh-2 were highly susceptible against R. similis. Characteistic of Java clone that esistance and Aceh clone that shyhtly susceptible to R. similis may be useful for providing new vaieties with high content of oil and resistant to nematode.
PERBAIKAN REKOMENDASI PAKET PHT UNTUK PENGENDALIAN HAMA Helicoverpa armigera Hbn. PADA TANAMAN KAPAS SKI HADIYANI; DA SUNARTO; A. A A. GOTHAMA; S. A. WAHYUNI
Jurnal Penelitian Tanaman Industri Vol 9, No 2 (2003): Juni, 2003
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v9n2.2003.63-69

Abstract

Rekomendasi pengendalian hama terpadu (PUT) hama utama lanaman kapas, akan tetapi secara tcknis dan ekonomis masih sulit diterapkan oleh petani. Unluk memperbaiki rekomendasi itu maka dikaji efisiensi pemanfaatan parasitoid Trichogrammatoidea armigera N&N. HeUcoverpa armigera nuclear polyhedrosis virus (//oNPV). dan insektisida nabati serbuk biji mimba (SBM) terhadap // armigera. Penelitian dilaksanakan pada musim lanam 199899 di kebun Instalasi Penelitian Tanaman Tembakau dan Serat, Asembagus, Situbondo, Jawa Timur. Rancangan penelitian adalah acak kelompok dengan 6 perlakuan, dan 3 ulangan Ukuran plot adalah 50 m x 50 m Pada rekomendasi standar rakitan komponen PITT, perlakuan yang ditambahkan adalah (1) penyemprotan SBM. (2) pelepasan T. armigera ■ penyemprotan SBM, (3) pelepasan '/'. armigera ' penyemprotan insektisida kimia, (4) penyemprotan faNPV I penyemprotan insektisida kimia. (5) penyemprotan insektisida kimia, dan (6) lanpa perlakuan (kontrol). Penyemprotan SUM, //oNPV, dan insektisida kimia dilakukan apabila populasi ulat // armigera mencapai ambang pengendalian (4 tanaman lerinfestasi per 25 tanaman). dan pelepasan 7 armigera dilakukan apabila populasi telur //. armigera mencapai ambang pelepasan (25 telur per 25 tanaman) Penyemprotan SBM, pelepasan T. armigera dan penyemprotan //oNPV dapat ditambahkan pada rekomendasi standar rakitan komponen PHT. Penambahan penyemprotan SBM pada rekomendasi standar rakitan komponen PITT adalah paling cisien diantara tiga komponen PITT yang ditambahkan, memberikan eisiensi (Marginal Regional Rate of Return. MRR) scbasar 3.64 dengan produktivitas kapas berbiji 1 562 kgha (meningkatkan 392 kg atau 25%) dan biaya penyemprotan Rp 172 267 (menurun Rp 259 883 atau 60%).Kata Kunci : Azadirachla tndica A. Juss, Trichogrammatoidea armigera N&N, WoNPV, HeUcoverpa armigera Hbn . PITT, Gossypium hirsutum I. ABSTRACT Improved 1PM recomendation package for controlling Helicoverpa armigera Hbn. on cotonAn experimcnl on Ihe use of IPM components for controlling HeUcoverpa armigera Hbn. as ihe pests of cotton was conducted in Asembagus Research Station during cotton planting season 1998/99 Ihe objective of this experiment was to assess the usefulness of Trichogrammatoidea armigera N&N., //oNPV, and botanical insecticide (neem seed powder) in improving the IPM recommendation package, so that it can be adopted by farmers This experiment was arranged in randomized blok design with 6 treatments and 3 replicates. Ihe size of each plot was 50 m X 50 m Ihe treatments comprise: (1) neem spray; (2) T. armigera release neem spray; (3) T. armigera release i insecticide spray; (4) //(A'I'V spray I chemical. (5) chemical; and (6) control (unlrcatment) were added lo the standar IPM recommendation package. Neem, /YoNPV, and chemical spray was done whenever population of H. armigera larvae reached action threshold (4 infected plants 25 plants) and release of T. armigera was done whenever population of H. armigera eggs reached "release threshold" (25 cggs/25 plants) Neem seed powder spray, T. armigera release, and //oNPV spray could be added to (he standar IPM recomendation package. Ihe most efficient among this three components was neem seed powder spray. Which gives eicjency Marginal Rate of Return (MRR) 3 64, cotton seed productivity I 562 kgha (increase 392 kg or 25%) and spraying cost Rp 172 267 (decrease Rp 259 883 or 60%).Key words: Azadirachla indica A. Juss., Trichogrammatoidea armigera N&N, 7/aNPV, Helicoverpa armigera Hbn , IPM, Gossypium hirsutum I.
RESPON KAPAS (Gossypium hirsutum L.) TERHADAP PUPUK KALIUM PADA VERTISOL DI BANGKALA JENEPONTO PETER TANDISAU; MUHAMMAD BASIR NAPPU; M. AZIS BILANG
Jurnal Penelitian Tanaman Industri Vol 4, No 4 (1998): November, 1998
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v4n4.1998.119-123

Abstract

Response of cotton (Gossypium hirsutum I.) to potassium fertilizer at vertisol Bangkala JenepontoThe study on the response of cotton o potassium on upland was carried out in vertisol Bangkala Jeneponto, South Sulawesi. The experiment was held on January 1994 to July 1994. The teatments consisted of seven rates of Potassium fertilizers viz : 0, 15, 30, 45, 60, 75 and 90 kg K20/ha or equal to 0, 25, 50, 75, 100, 125 and 150 kg KCI/ha. The teatments were arranged in a randomized block design, with four replications. The esearch results indicated that cotton responded to potassium applications. Potassium ertilizer could improve cotton esponded o potassium applications. Potassium ertilizer could improve coton growth and yield component, and seed cotton production. The response curve was still linear. The moe KCI applied, the better the cotton growth and yield component, and the seed cotton yield. The application of Potassium ertilizer 125 kg KCI or 75 kg KjO/ha on coton planted on vertisol Bangkala Jenepono produced seed coton yield I 911 kg/ha.
KORELASI SIFAT FISIK DAN KIMIA TANAH DENGAN INTENSITAS PENYAKIT BUSUK PANGKAL BATANG TANAMAN LADA / Correlation of Physical and Chemical Soil Characteristics with Intensity of Foot Rot Disease of Black Pepper La Ode Santiaji Bande; Abdul Wahab; Bambang Hadisutrisno; Susamto Somowiyarjo; Bambang Hendro Sunarminto
Jurnal Penelitian Tanaman Industri Vol 22, No 2 (2016): Juni, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n2.2016.63-70

Abstract

Foot rot disease of black pepper caused by Phytophthora capsici is the most destructive disease in Southeast Sulawesi. The disease intensity is 61.2%. This disease is difficult to control because the pathogen is soil borne and influenced by various complex environmental conditions. The study aimed to determine the correlation of physical and chemical soil characteristics with disease intensity of foot rot of black pepper. Disease intensity was assessed from the subplots of black pepper plantation of 15x15 m2 consisting of 36-40 plants. Soil samples were randomly taken from the rhizosphere of the plants in each sub plot, mixed and taken as a composite. The physical and chemical analyses were conducted in the laboratory of Physics and Chemistry of the Faculty of Agriculture, Gajah Mada University. Disease intensity and soil characteristics were analyzed its correlation using a path analysis. The results showed that soil fertility in the study area was low. The path analyses indicated that physical and chemical properties that correlated with low disease intensity were high cation exchange capacity (CEC), base saturation (BS), potassium and phosphor available as well as moisture at field capacity, whereas those induced disease development were high content of clay fraction, sand fraction, porosity, total N, C-organic and salinity. The control strategy for foot rot disease in Southeast Sulawesi was possibly by increasing CEC, BS, potassium and phosphor available, as well as by improvement of soil texture that can lower high fraction of clay, sand and soil porosity
EVALUASI KETAHANAN AKSESI KENAF (Hibiscus cannabinus L.) TERHADAP PENYAKIT LAYU Fusarium oxysporum SCHLECT GEMBONG DALMADIYO; CECE SUHARA; SUPRIYONO SUPRIYONO; SUDJINDRO SUDJINDRO
Jurnal Penelitian Tanaman Industri Vol 6, No 2 (2000): September, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v6n2.2000.29-32

Abstract

Evaluation on the resistance of kenaf accessions (Hibis¬ cus cannabinus /..) to Fusarium oxysporum SchlectResistant variety is one of the most important components controlling of fusarium wilt disease on kenaf caused by Fusarium oxysporum Schlect. To ind out resistant variety an evaluation on kenaf accessions was conducted in the laboratory and screen house of Phytopathology, RITFC, Malang in June-December 1997. The results of the selection on 77 accessions showed that 41 accessions were highly resistant, 1 2 accessions were resistant, 7 accessions were moderate, 12 accessions were susceptible, and 5 accessions were highly susceptible Three resistant and highly resistant accessions were namely 85-9-73, DS/005 H, and FJ/004 He could inhibit F. oxysporum growth about 23.40- 32.43 mm and its discolorisation about 0.0-13.4%.
STATUS TANAMAN, PRODUKSI DAN PENGGUNAAN CENGKEH SYAFRIL KEMALA
Jurnal Penelitian Tanaman Industri Vol 10, No 2 (2004): Juni 2004
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v10n2.2004.59-65

Abstract

Penelitian ini betujuan untuk mengetahui (1) luas dan keadaan lanaman pada status TBM, TM dan TR secara nyata, (2) pcndugaan produksi dan penggunaan cengkch "agregate" untuk tahun-tahun yang akan datang. Hasil yang diharapkan adalah dipeolehnya da(a yang lebih baik karena data BPS/Dirjenbun cenderung over estimate. Penelitian ini menggunakan mctode survci untuk pcndugaan luas dan produksi pada daerah sentra produksi (Sulawesi Utara, Jawa Tengah, dan Lampung), seta pendugaan penggunaan cengkch di Jawa Tengah dan Jawa Timur. Pada tiap propinsi diambil 3 kecamatan dan tiap kecamatan yang terpilih diambil 3 desa, dengan kiteria produksi tinggi, sedang dan rendah. Sedangkan penggunaan cengkch diambil secara purporsivc (terarah) atas kontribusi pabrik rokok. Jumlah petani sampel yang diambil 230 dan jumlah pabrik 9 buah (3 besar, 3 sedang dan 3 kecil). Penelitian dilakukan pada bulan April 2001 sampai Desember 2001. Analisis data dilakukan secara statistik dengan mctode kuadrat terkecil untuk pcndugaan produksi dan penggunaan (2) penghitungan status tanaman (TBM, TM, TR) dilakukan dengan metode matematis (pcrsamaan identitas). Hasil penelitian menunjukkan bahwa populasi lanaman yang belum menghasilkan (TBM) tidak ada, tanaman menghasilkan (TM) adalah 40% dan tanaman rusak/mati (TR) adalah 60%. Dari status TR yang berpcluang/potcnsial produktif hanya 34% dan 66% rusak berat. Dari status tanaman menghasilkan TM distibusinya 63.78% tcrgolong tua (>25 tahun). Produktivitas tanaman per ha makin menurun dan meningkat untuk per pohon. Data Disbun/BPS temyata over estimate ± 14% dibandingkan dengan data survci. Dari hasil pendugaan produksi dan penggunaan untuk tahun 2010 akan terjadi ekses demand yang cukup besar yang tidak dapat ditutupi oleh impor. Bila lidak terjadi perubahan teknologi dan kebijakan pemerintah akan terjadi kekurangan cengkch.Kata kunci : Cengkeh, status tanaman, produksi, penggunaanABSTRACT Status ofplant, production and usage of cloveThis research was aimed to find out (1) the size and situation of crop status at TBM. I'M and TR manifestly, ( 2) lo estimate the production and usage of clove " agregate" for the following years. The objective of Ihe research was lo obtain better data because the data of BPS/DG Estate tended to be over estimate. This research used survey method to estimate the size and the production of the clove area of central production (North Sulawesi, Central Java, and I .ampiiiig). and also to estimate the usage of clove in Central Java and East Java. At every province was taken 3 disticts and every district was taken 3 villages, with the criteria, high, medium, and low production. While the clove usage was taken by purposive of cigarete factory contibution. The number of farmers taken for sample were 230 and the number of factories were 9 (3 big, 3 medium and 3 small) The research was conducted in April 2001 to December 2001. Data analysis was conducted statistically with smallest square method for the estimating production and usage, while the enumeration of crop status (TBM, TM, TR) was conducted with mathematical method (equation of identity ). 'The result of the research indicated that there is no crop population which have not yet yielded (TBM) yielded crop (TM) was 40%, and damaged/death crop (TR) was 60%. From the TR status which have oppotunity to be productive was only 34% and 66% damaged. Of yielded crop status (TM) the distribution was 63.78% was old (>25 year). Crop productivity per ha decrease, however the productivity per plant increased. Data of BPS/DG estate was over estimate ± 14% compared to survey data. Fom the estimation of the production and usage of clove, for the 2010 Ihere will be highly demand which cannot be met by import. If there were no suppoting technology and government policy, there will be lack of clove supply.Key words : Clove, crop status, usage and production
KETAHANAN BEBERAPA KELAPA HIBRIDA TERHADAP PENYAKIT BUSUK PUCUK HENDRIK F. MANGINDAAN; MIFTAHORRAHMAN MIFTAHORRAHMAN; HENGKY NOVARIANTO
Jurnal Penelitian Tanaman Industri Vol 5, No 2 (1999): September, 1999
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n2.1999.46-50

Abstract

Resistance of some hybrid coconuts to bud rot diseaseBud rot disease by Phytophthora palmivora has caused significant loss lo coconut farmers because the pathogen could kill the palms in relative short time. This disease mainly attacks hybid coconut of PB-121. To anticipate the coconut development in the futue, it is necessary to find supeior coconut or hybrid coconut which resistant to bud rot disease. This research was conducted to test the resistance of 25 combinations of hybrid coconuts to the disease. The research used randomized block design with 25 kinds of hybids, 19 replications with 3 palms for each eplication. The trial was done at Paniki Instalation, North Sulawesi The hybrids were planted in November 1993. The observation of disease for 5 years showed that bud rot symptoms was irstly irst appeared at 40 months old hybrids i.e. 4 palms of the cross GKN x PYT, GRA x PYT, GHJ x WAT, and GHJ x DSA. Until ive yean ater planting there were 17 palms died because of the bud rot. In general, the hybrids of the crossing which used male parent of WAT wee the most susceptible. While the hybrids of the crossing of GSK as a female paent showed resistant to bud rot disease.
EVALUASI KETAHANAN HIBRIDA SOMATIK NILAM TERHADAP PENYAKIT LAYU BAKTERI (Ralstonia solanacearum) NASRUN NASRUN; NURMANSYAH NURMANSYAH; HERWITA IDRIS
Jurnal Penelitian Tanaman Industri Vol 15, No 3 (2009): September 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v15n3.2009.110-115

Abstract

ABSTRAKPenelitian evaluasi ketahanan hibrida somatik nilam terhadappenyakit layu bakteri (Ralstonia solanacearum) telah dilaksanakan dilaboratorium dan rumah kaca KP Laing Solok dari bulan Januari sampaiDesember 2007. Penelitian bertujuan untuk mendapatkan nomor hibridasomatik nilam yang tahan terhadap penyakit layu bakteri. Kegiatanpenelitian meliputi pengambilan sampel tanaman nilam terinfeksi penyakitlayu bakteri di lapangan, dan isolasi dan perbanyakan isolat bakteripatogen di laboratorium. Uji patogenisitas isolat bakteri patogen pada bibitnilam dilaksanakan di rumah kaca. Selanjutnya perbanyakan bibit nilamhibrida somatik, inokulasi bibit dengan bakteri somatik, dan inkubasi bibittersebut dilaksanakan di rumah kaca. Penelitian ini menggunakan beberapanomor nilam hibrida somatik sebagai perlakuan yang disusun dalamrancangan acak lengkap (RAL) dengan tiga ulangan. Parameter yangdiamati adalah masa inkubasi menunjukkan gejala awal penyakit untuk ujipatogenisitas isolat bakteri patogen. Masa inkubasi menunjukkan gejalaawal penyakit dan kematian serta intensitas penyakit. Selanjutnya jugadiamati pertumbuhan tanaman dan produksi daun basah untuk pengujianketahanan nomor hibrida somatik nilam terhadap bakteri patogen. Hasilpenelitian menunjukkan bahwa hibrida somatik 2IV/4 dan 9II/21 sertavarietas Girilaya sampai akhir pengamatan tidak menampakkan gejalapenyakit. Sementara itu hibrida somatik 2IV/6; 9II/34; 2IV/9; 9IV/3; dan9IV/6 menunjukkan gejala awal penyakit (118,5 – 133,6 hari setelahinokulasi ”HSI”) dan kematian bibit (130,5 – 182,2 HSI) paling lamadengan intensitas penyakit (29,6 – 48,2%) lebih rendah dibandingkandengan hibrida somatik lainnya. Selanjutnya, penyumbatan pembuluhkayu sangat rendah yaitu 5,20 – 9,50% pada bagian akar, tetapi padabagian pangkal batang dan pucuk tidak ada penyumbatan. Pertumbuhandan produksi daun basah lebih tinggi dibandingkan dengan hibrida somatiklainnya, yakni tinggi tanaman 78,00 – 104,00 cm; jumlah daun 66 – 112daun, dan produksi daun basah 55,00 - 99,19 g. Sebaliknya hibridasomatik 9IV/4; 9II/23; 9II/33; 9IV/14; dan 2IV/1 memperlihatkan gejalaawal penyakit (32,3 -105,84 HSI) dan kematian bibit nilam (41,5 – 125,0HSI) lebih cepat, dan intensitas penyakit lebih tinggi (75 – 100%)dibandingkan hibrida somatik lainnya termasuk varietas Sidikalang.Penyumbatan pembuluh kayu oleh bakteri patogen sebesar 12,46 – 31,25%pada bagian akar, 6,10 – 22,50% pada bagian batang, dan 4,17 – 6,25%pada pucuk. Begitu juga dengan pertumbuhan dan produksi daun basahlebih rendah dibandingkan dengan hibrida somatik lainnya, dengan tinggitanaman 42,20 – 61,85 cm, jumlah daun 24 - 37 daun, dan produksidaun basah 18,00 - 41,20 g. Berdasarkan hasil penelitian ini terpilih duanomor hibrida somatik yaitu (2IV/4 dan 9 II/21) yang tahan terhadappenyakit layu bakteri, dan lima nomor hibrida somatik yaitu (9IV/6, 9IV/3,9II/34, 2IV/9, dan 2IV/6) yang toleran terhadap penyakit layu bakteri dirumah kaca.Kata kunci : Pogostemon cablin Benth., nilam, penyakit layu bakteri,evaluasi ketahanan, hibrida somatikABSTRACTEvaluation on the Resistance of Hybrid Somatic PatchouliPlant to Bacterial Wilt Disease (Ralstonia solanacearum)The study on the resistance evaluation of somatic hybrid patchouliplant to bacterial wilt disease (Ralstonia solanacearum) had beenconducted in green house KP.Laing Solok from January to December2008. The objective of this study was to find the somatic hybrid numbersof patchouli plants resistant to bacterial wilt disease. The studied activitieswere collection of patchouli plant that was infected by bacterial wiltdisease in the field. Isolation and culture of the pathogen bacterial wereconducted in the laboratory and virulence test of pathogen bacterial onpatchouli plant was in the green house. In addition, sub culture of thesomatic hybrid seeds of patchouli plants, inoculation of these seeds bybacterial pathogen, and incubation of them were done at green house. Thestudy used some numbers of somatic hybrid as treatments that werearranged in completely randomized design (CRD) with three replicates. Asparameters were incubation period showing the first disease symptom forpathogenicity test of bacterial pathogen. Incubation period showed thefirst symptom died plant and diseases intensity. Plant growth and leafproduction were also observed for the study of number resistance ofsomatic hybrid patchouli plant to bacterial pathogen. The results showedthat somatic hybrid 2 IV/4 and 9 II/21 and Girilaya varieties did not showany symptoms. However, somatic hybrid 2IV/6, 9II/34, 2IV/9, 9IV/3, and9IV/6 showed the first symptom (118.5 – 133.6 days after inoculation”DAI”) and dead period (41.5 – 125.0 DAI) were the longest and thedisease intensity (29.6 – 48.2%) was lower than other somatic hybrid.Occluded xylem vessel by pathogen bacterial on root was lowest 5.20 –9.50%, but it was not found on base and young stems. They are alsohigher in the growth and leaf production than another somatic hybridpatchouli plant with 75.00 – 104.00 cm plant height, 66 -112 leaves/plant,and fresh leaf production of 55.00 – 99.19 g/pot. However, somatic hybrid9 IV/4, 9 II/23, 9 II/33, 9 IV/14, and 2 IV/1 showed earlier diseasesymptom, quicker seed death and higher disease intensity (75 -100%) thanother somatic hybrid patchouli plants including Sidikalang variety. Inaddition they had occluded xylem by bacterial pathogen 12.46 - 31.25% onthe root, 6.10 - 22.50% on the stem, and 4.17 – 6.25% on young stem.Plant growth and leaf production were lower than other somatic hybridpatchouli plants as of was 42.20-61.85 cm plant height; 24-37 leaves/plant,and fresh leaf production 18.00-41.20 g/pot. According to these results,experiment selected two numbers of somatic hybrid (2IV/4 and 9II/21)which were resistant to bacterial wilt disease, and five numbers of somatichybrid (9 IV/6, 9IV/3, 9II/34, 2IV/9, and 2IV/6) that were tolerant tobacterial wilt disease in the glass house.Key words: Pogostemon cablin Benth., patchouli, bacterial wilt disease,resistant evaluation, somatic hybrid patchouli plant
METODE EKSTRAKSI DNA PADA Jatropha spp. TANPA MENGGUNAKAN NITROGEN CAIR / DNA Extraction Method of Jatropha spp. without Liquid Nitrogen Kristianoto Nugroho; Rerenstradika T. Terryana; Habib Rijzaani; Puji Lestari
Jurnal Penelitian Tanaman Industri Vol 22, No 4 (2016): Desember, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n4.2016.159-166

Abstract

Physic nut is one of potential plant which produces biofuel. It is necessary to do an intensive breeding program either conventionally or molecularly based to develop new varieties of physic nut. All this time the physic nut DNA extraction methods always used liquid nitrogen to destroy the plant tissue. Liquid nitrogen is not always available, especially in remote areas. Physic nut has high latex content which makes extraction process more difficult. The aim of this study was to find the technique of DNA extraction which can give a good result without using liquid nitrogen. This research was conducted in Molecular Biology Laboratory, ICABIOGRAD Bogor from November to Desember 2015. There were five Jatropha species from Thailand which used in this research e.g. Jatropha curcas, Jatropha podagrica, Jatropha gossypifolia, Jatropha multifida, and Baliospermum solanifolium. The extraction method used modification CTAB by addition of PVP, sodium metabisulphite, sucrose and ascorbic acid. The young leaves were used as the part to be extracted. The results showed that the modified method could produce a good quality and quantity of DNA. The banding pattern of DNA amplification clearly visible under UV light. This method can reduce the dependency of liquid nitrogen. AbstrakEkstraksi DNA merupakan salah satu ta hap penting dalam kegiatan pemuliaan berbasis molekuler. Jarak merupakan salah satu tanaman dengan kandungan getah cukup tinggi sehingga proses ekstraksi DNA cukup sulit dilakukan. Selama ini metode ekstraksi yang dikembangkan umumnya menggunakan nitrogen cair untuk menghancurkan jaringan tanaman. Nitrogen cair merupakan senyawa yang cukup sulit untuk didistribusikan ke laboratorium yang letaknya jauh dari kota. Tujuan penelitian ini adalah untuk memperoleh teknik ekstraksi DNA jarak yang mampu menghasilkan DNA dengan kualitas dan kuantitas yang baik tanpa menggunakan nitrogen cair. Penelitian ini dilaksanakan di Laboratorium Biologi Molekuler, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian (BB Biogen), Bogor pada bulan November hingga Desember 2015. Terdapat lima spesies jarak asal Thailand yang digunakan pada penelitian ini yaitu Jatropha curcas, Jatropha podagrica, Jatropha gossypifolia, Jatropha multifida, dan Baliospermum solanifolium. Ekstraksi DNA dilakukan melalui modifikasi buffer CTAB melalui penambahan senyawa PVP, natrium metabisulfit, sukrosa dan asam askorbat. Bagian tanaman yang digunakan berupa daun yang masih muda. Hasil penelitian menunjukkan DNA jarak yang diekstraksi dengan metode ini mempunyai kualitas dan kuantitas yang baik serta mampu diamplifikasi dengan baik. Metode ini mampu mengurangi ketergantungan terhadap nitrogen cair yang ketersediaannya terbatas di laboratorium yang lokasinya jauh dari kota.Kata kunci: Jatropha spp., ekstraksi, DNA, nitrogen cair

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