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Jurnal Penelitian Tanaman Industri
Published by Kementerian Pertanian
ISSN : 08538212     EISSN : 25286870     DOI : -
Core Subject : Engineering,
Industrial & Manufacturing Engineering
Jurnal Penelitian Tanaman Industri merupakan publikasi ilmiah primer yang memuat hasil penelitian primer komoditas perkebunan yang belum dimuat pada media apapun, diterbitkan oleh Pusat Penelitian dan Pengembangan Perkebunan, DIPA 2011 terbit empat kali setahun.
Arjuna Subject : -
Articles 552 Documents
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DEGRADASI DINDING SEL Phytophthora capsici OLEH ENZIM KARBOSI METIL SELULASE ASAL Trichoderma harzianum KARDEN MULYA; MELLY HARMEN
Jurnal Penelitian Tanaman Industri Vol 9, No 2 (2003): Juni, 2003
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v9n2.2003.74-78

Abstract

Phytophthora capsici Leonian adalah patogen penyebab penyakit busuk pangkal batang lada (Piper nigrum L). Trichoderma harzianum Rifai merupakan agen hayati yang cfektif dan menyebabkan lisis miselia P. capsici. Penelitian ini bertujuan unluk mcngc(ahui peran enzim karboksimetilselulase (CMC-ase) yang diproduksi oleh T. harzianum dalam mendegradasi dinding sel P capsici. Penelitian terdiri atas tiga aktivitas yaitu (a) deteksi produksi enzim CMC-ase, (b) hidrolisis dinding sel P. capsici. dan (c) penggunaan siapan kasar dinding sel (SKDS) P. capsici oleh T. harzianum sebagai satu-satunya sumber karbon dalam media tumbuhnya Aktivitas enzim dideleksi secara kualitatif dengan membandingkan zona bening yang terbentuk pada medium karboksi metil selulosa (CMC) yang diperlakukan dengan satu tetcs iltrat kultur 7 harzianum dan diwarnai dengan larulan congo red, sedangkan aktivitas CMC-ase secara kuantitatif diukur sebagai nilai setara glukosa yang terlepas dari substrat setelah diinkubasi dengan ekstrak kasar CMC-ase. Enzim CMC-ase dickstrak dari kultur filtrat T. harzianum F.51 melalui pengendapan protein dengan 85% ammonium sulfat pada suhu 4°C diikuti dengan sentrifusi dan dialisis. Aktivitas spcsifik enzim tersebut dalam mendegradasi CMC (30,29 unif//g protein) lebih rendah dari aktivitas enzim selulase komersial (97.18 unit/^g protein). Enzim selulase komcrsial dan ekstrak enzim dari kultur 7 harzianum juga dapat menghidrolisis SKDS P. capsici N2 dengan aktivitas masing-masing 31.18 unit dan 19.26 unit. Isolat F-51 tumbuh dan menghasilkan aktivitas enzim serupa manakala karboksimclil selulosa pada media tumbuh diganti dengan SKDS sebagai sumber karbon tunggal Hasil ini menunjukkan bahwa CMC-ase berperan penting dalam mckanisme antagonis T. harzianum terhadap /' capsici. Penelitian lebih lanjut dalam mckanisme produksi enzim ini berpeluang untuk meningkatkan potensi agen hayati.Kata kunci : Piper nigrum L., Trichoderma harzianum Rifai, Phytophthora capsici. karbosimctilsclulasc, agen hayati ABSTRACTDegradation of cell wall of Phytophthora capsici N2 by carboxy methyl cellulose <;/ Trichoderma harzianum ES1Phytophthora capsici l-eonian is a causal agent of foot rot disease on black pepper (Piper nigrum L ). Trichoderma harzianum Rifai is an effective biocontrol agent and causes lyscs on P. capsici mycelium This experiment was aimed to study the role of carboxymethylccllulose (CMC- ase) produced by T. harzianum in degrading P. capsici cell wall. The experiment was composed in three activities (a) detection of the CMC-ase enzyme production, (b) degradation of P. capsici cell wall by crude extract of the enzyme, and (c) utilization of crude cell wall preparation (cwp) of P. capsici by T. harzianum as single carbon source in its growth medium CMC-ase activity was detected qualitatively on carboxymethylccllulose (CMC) medium, and quantitatively it was measured as glucose equivalent released from substrate after treated with crude extract of CMC-ase. CMC-ase was extracted from culture iltrate of T. harzianum F-51 by precipitation of protein with 85% ammonium sulphate at 4°C followed by dialysis with distilled water and liophyllizcd. Activity of the extracted enzyme on degradation of CMC (30.29 unil-'/jg protein) was lower than activity of commercial cellulose (97.18 united g protein) Commercial cclullosc and the extracted enzyme also degraded crude cell wall prepared (CWP) from P. capsici N2 as indicated with the presence of glucose equivalent released from CWP after incubation with the enzyme; with specific activity of 31.18 unit and 19.26 unit respectively. Trichoderma harzianum grown in medium suplemented with the crude cell wall of P. capsici as a single carbon source produced CMC-ase. Those results indicated that CMC-ase has important role on parasitism of T. harzianum on P. capsici Further investigation is required to elucidate mechanism of production of CMC-ase in T. harzianum for improvement of biocontrol activity of the fungus.Key words: Piper nigrum L., Trichoderma harzianum Rifai, Phytophthora capsici, carboxymethylcellulosc, biocontrol
KARAKTERISTIK FISIK DAN KIMIA DARI PRODUK BIOINDUSTRI CANGKANG JAMBU METE (Anacardium occidentale) / Physical and Chemical Characteristics of Bioindustry Products of Cashewnuts Shell (Anacardium occidentale) Andi Saenab; K. G. Wiryawan; Retnani Y.; E. Wina
Jurnal Penelitian Tanaman Industri Vol 22, No 2 (2016): Juni, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n2.2016.81-90

Abstract

The shell of the cashew nut industry waste has the potential to produce a product that is biofat bioindustry, biochar and biosmoke. The research aims to produce three products bioindustry, observe the physical and chemical characteristics. Eggshell processed into a particle size of 1 cm and 2 mm size, extracted with hexane and extraction evaporated to obtain Biofat. The residue biofat charred shell after the extract to produce Biochar and Biosmoke. Proximate analysis performed on fresh shells, biofat, biochar and biosmoke. The result biofat yield increased three-fold when the size of the shell is reduced to 2 mm (17.81 g/100g BK), while biochar and biosmoke increased two-fold when the size of the shell is enlarged 1 cm (30.82 g/100g BK and 5:13 g/100g BK). Biofat containing coarse fat and high total phenol (94.43 g/100g biofat, 46 mg/100g biofat) and total phenol containing biosmoke quite high (7.2 mg/100g biosmoke) on a large particle size. The results of GC-MS analysis showed biofat and biosmoke acidic anacardic high (74%) and biochar contains minerals especially high ash content (6:56%), Ca (0.69%), and P (12:23%) 2-fold increase in the particle size of the shell smaller than the large. Mineral Fe (695 mg/kg) more contained shells of large particles, whereas the characteristic biosmoke have a high acid content (pH 3). Cashew nut shell bioindustry products have specific physical and chemical characteristics to be used in various fields including livestock, agriculture or food.
INDUKSI TUNAS TABAT BARITO (Ficus deltoidea JACK) SECARA IN VITRO MENGGUNAKAN BENZIL ADENIN (BA) DAN NAPHTHALENE ACETIC ACID (NAA) NATALINI NOVA KRISTINA
Jurnal Penelitian Tanaman Industri Vol 15, No 1 (2009): Maret 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v15n1.2009.33-39

Abstract

ABSTRAKTabat barito (Ficus deltoidea Jack), merupakan salah satu tanamanobat yang dikategorikan langka dan digunakan sebagai bahan afrodisiakwanita. Perbanyakan tanaman secara in vitro dilakukan untuk mendapatkanbahan tanaman dalam jumlah banyak. Penelitian bertujuan untukmendapatkan media terbaik tabat barito dan telah dilakukan di laboratoriumkultur jaringan Plasma Nutfah dan Pemuliaan Balittro, pada bulanJanuari sampai dengan Desember 2007. Penelitian ini dilakukan dalam tigatahap, yaitu : 1) respon tunas pada media perbanyakan, menguji mediamultiplikasi tunas dengan media sitokinin tunggal yaitu : MS + BenzilAdenin (BA) 0,5; 1,0; 1,5 dan 2,0 mg/l; tahap 2) respon tunas pada mediakombinasi sitokinin dan auksin, yaitu : MS + BA 0,5 mg/l + NAA 0,1mg/l; MS + BA 0,5 mg/l + NAA 0,5 mg/l; MS + BA 1,0 mg/l + NAA 0,1mg/l dan MS + BA 1,0 mg/l + NAA 0,5 mg/l. Tahap 3) Daya multiplikasidan penampilan tunas setelah subkultur pada media yang sama. Masingmasingpercobaan disusun dengan rancangan acak lengkap, dan terdiri atas5 ulangan. Parameter pengamatan meliputi jumlah tunas, tinggi tunas danjumlah ruas serta penampilan visual. Hasil penelitian menunjukkan bahwapada tahap pertama, penggunaan media MS + BA 0,5 menghasilkanjumlah tunas yang paling banyak dibandingkan yang lain, tetapi tidakberbeda nyata pada jumlah ruas dan tinggi eksplan. Sementara pada tahapdua, jumlah tunas terbaik didapat pada media dengan auksin rendah baikpada kombinasi sitokinin rendah dan tinggi. Sementara untuk jumlah ruasmedia terbaik adalah media dengan konsentrasi BA tinggi yangdikombinasi dengan NAA. Untuk tinggi tunas, media terbaik adalah MS +BA 1,0 mg/l + NAA 0,5 mg/l, tapi ditemukan eksplan yang menguning.Pada tahap ketiga, dari hasil subkultur kembali terlihat bahwa tunas yangbersumber dari pucuk pertumbuhannya baik sementara tunas yang berasaldari ruas ke-2 dan 3 sebagian menguning.Kata kunci : Ficus deltoidea Jack, tunas, induksi, in vitro, BA, NAAABSTRACTIn vitro shoot induction of Mistleteo fig (Ficus deltoideaJack) in Murashige & Skoog (MS) media with addition ofBA and NAAMistleteo fig (Ficus deltoidea) is one of endangered medicinalplants and used for female aphrodisiac. In vitro multiplication of the plantwas done to find a number of shoots. This experiment was conducted intissue culture laboratory of Germplasm and Breeding of IMACRI fromJanuary to December 2007, and aimed to find best media for shootmultiplication. This experiment was carried out in three steps: step 1)shoot respon in multiplication media using single cytokinin : MS + BA(0.5; 1.0; 1.5 and 2 mg/l); step 2) shoot respon in multiplication media ofcombined cytokinin and auxin : MS + BA 0.5 mg/l + NAA 0.1mg/l; MS+ BA 0.5 mg/l + NAA 0.5 mg/l; MS + BA 1.0 mg/l + NAA 0.1 mg/l andMS + BA 1.0 + NAA 0.5 mg/l; and step 3) viability and visualization ofthe shoots after subcultured in the same media. The experiment wasarranged using completely randomized design with 5 replicates. Theparameters observed were of shoots and nodes, shoot height andperformance. The results in the first step showed that MS + BA 0.5 mg/lmedia resulted in the highest number of shoots, but they were notsignificantly different in the number of nodes and shoots height. In thesecond step, highest number of shoots was found using low concentrationof auxin combined with low and high concentration of cytokinin. Bestmedium for number of nodes was MS with high concentration of BAcombined with NAA. For shoot height, the best medium was MS + BA0.1 mg/l + NAA 0.5 mg/l, but the shoots turned yellow. In the third step,after subcultured, the shoots originated from plant tips performed well,however, those taken from second and third inter nodes partially turnedyellow.Key words : Ficus deltoidea Jack, shoot, induction, in vitro, BA, NAA
SUSTAINABILITY OF TEMANGGUNG COFFEE FARMING SYSTEM IN THE PERSPECTIVE OF GEOGRAPHICAL INDICATIONS I Ketut Ardana
Jurnal Penelitian Tanaman Industri Vol 25, No 2 (2019): Desember, 2019
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v25n2.2019.69-80

Abstract

Geographical Indications (GIs) certificate is required to protect specific flavors of crops in the given region. The study aimed to analyze the sustainability of the coffee farming system basis of Sindoro-Sumbing Java Arabica Coffee and Temanggung Robusta Coffee from the perspective of GIs protection. The study used survey methods with data collection techniques: (1) literature studies, interviews, and discussions with stakeholders, and (2) observation of the application of coffee cultivation and processing. The analytical methods used were (1) analysis of technical and institutional performance, and economic benefits with cross-tabulation and descriptive interpretation, and (2) analysis of the sustainability of coffee farming system using a multidimensional scaling approach. The results showed that the management of coffee farming in the Temanggung Regency was quite sustainable from the perspective of GIs protection with an index value of 66.88. The six dimensions of sustainability, showing fairly sustainable performance with an index of 59.22-74.99. This indicated that a comprehensive improvement is required to sustain the GI protection in Temanggung Regency, i.e., ecology, economy, ethics, institutions, social, and technology. Sensitive lever factors to improve the performance of each dimension are (1) adaptation to climate influence, land conservation, and cropping patterns for ecological dimensions, (2) commodity alternatives and price stability for economic dimensions, (3) logo inclusion on packaging, environmental maintenance, and the authenticity of products for ethical dimensions, (4) optimizing the role of GIPC for institutional dimensions, (5) involvement of stakeholders in the social dimension, and (6) improvements in harvesting methods for technological dimensions.Keywords:   Lever factors, arabica coffee, robusta coffee AbstrakSertifikat Indikasi Geografis (GI) diperlukan untuk melindungi rasa tanaman tertentu di wilayah tertentu. Penelitian ini dilakukan dari bulan Desember 2017 hingga Mei 2018, bertujuan untuk menganalisis keberlanjutan usahatani kopi Arabika Java Sindoro-Sumbing dan Kopi Robusta Temanggung dalam perspektif perlindungan IG menggunakan metode survei dengan teknik pengumpulan data: (1) studi pustaka, wawancara dan diskusi dengan pemangku kepentingan, dan (2) observasi penerapan budidaya dan pengolahan kopi. Metode analisis yang digunakan meliputi: (1) Analisis kinerja teknis,  kelembagaan, dan manfaat ekonomi dengan tabulasi silang dan interpretasi secara deskriptif, dan (2) Analisis keberlanjutan menggunakan pendekatan multidimensional scaling. Hasil penelitian menunjukkan bahwa pengelolaan usahatani kopi di Kabupaten Temanggung cukup berkelanjutan dari perspektif perlindungan IG dengan nilai indeks 66,88. Keenam dimensi keberlanjutan, menunjukkan kinerja cukup berkelanjutan dengan indeks 59,22-74,99 mengindikasikan bahwa untuk meningkatkan keberlanjutan perlindungan IG kopi di Kabupaten Temanggung masih perlu perbaikan pada dimensi ekologi, ekonomi, etika, kelembagaan, sosial, dan teknologi. Faktor pengungkit yang sensitif untuk memperbaiki kinerja masing-masing dimensi adalah: (1) adaptasi terhadap pengaruh iklim, konservasi lahan, dan pola tanam untuk dimensi ekologi, (2) alternatif komoditas dan stabilitas harga untuk dimensi ekonomi, (3) pencantuman logo pada kemasan, mitigasi lingkungan, dan keaslian produk untuk dimensi etika, (4) mengoptimalkan peran MPIG untuk dimensi kelembagaan, (5) soliditas komunitas “Sahabat Kopi” dan keterlibatan stakeholder untuk dimensi sosial, serta (6) perbaikan cara panen untuk dimensi teknologi.Kata kunci: faktor pengungkit, keberlanjutan, kopi arabika, kopi robusta, indikasi geografis.
AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI DARI DERIVAT METIL EKSTRAK ETANOL DAUN GAMBIR (Uncaria gambir) IRMA KRESNAWATY; ACHMAD ZAINUDDIN
Jurnal Penelitian Tanaman Industri Vol 15, No 4 (2009): Desember 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v15n4.2009.145-151

Abstract

ABSTRAKBanyak tanaman yang dilaporkan memiliki kandungan senyawabahan aktif antioksidan dan antibakteri. Salah satu tanaman Indonesia yangmemiliki aktivitas ini adalah gambir (Uncaria gambir). Pada penelitian ini,ekstrak etanol daun gambir diubah menjadi derivat metilnya untukmembuatnya lebih larut dalam lemak dan diamati pengaruh derivatisasitersebut terhadap aktivitas antioksidan di laboratorium kimia organik danpengujian aktivitas antibakteri di laboratorium mikrobiologi UniversitasPadjadjaran. Penelitian ini dilaksanakan di laboratorium Kimia OrganikJurusan Kimia dan laboratorium Mikrobiologi Jurusan Biologi UniversitasPadjadjaran dari bulan Desember 2004 - Juli 2005. Ekstrak gambirdimetilasi  menggunakan  dimetil  sulfat  (DMS)  dan  dimurnikanmenggunakan kromatografi kolom dengan pelarut bergradien (kloroform :metanol = 99:1 ; 98:2 ; 95:5 ; 80:20 ; 70:30; dan 50:50 v/v) dan kemudianmenggunakan kloroform : metanol = 99 : 1 v/v. Aktivitas antioksidanmenunjukkan penurunan yang tampak dari peningkatan, yaitu : IC 50  13,41ppm untuk ekstrak etanol menjadi 121,81 ppm untuk hasil metilasi.Aktivitas antibakteri juga menunjukkan penurunan setelah dimetilasikarena adanya penurunan diameter hambat pertumbuhan bakteri. Duaisolat (isolat 1 dan 2) yang diperoleh dari hasil pemurnian dikarakterisasimenggunakan spektrofotometer UV-Visible dan FT-IR. Hasil yangdiperoleh mengindikasikan adanya senyawa fenolik yang ditunjukkan olehregang –OH pada 3445 dan 3448 cm -1 dan regang CH aromatik pada 3010dan 3030 cm -1 . Isolat 1 memiliki aktivitas antioksidan dan antibakteri yanglebih tinggi dibandingkan isolat 2.Kata kunci : Uncaria gambir, derivat metil, antibakteri dan aktivitasantioksidanABSTRACTThe antioxidant and antibacterial activities of ethanolextract of gambir leaves (Uncaria gambir)There are many plants in Indonesia reported to contain antioxidantand antibacterial substances. One of them having these activities is gambir(Uncaria gambir). In this research, ethanol extract of gambir leaves waschanged into its methyl derivate in order to make it more soluble in fats.The effect of the derivatization on antioxidant activity was observed atorganic chemistry laboratory and antibacterial activity was observed atmicrobiology laboratory of the Padjadjaran University. This research wascarried out in December 2004 to July 2005. Gambir extract wasmethylized using dimethylsulphate (DMS) and then purified usingcoloumn chromatography with gradient solvents (chloroform : methanol =99:1; 98:2; 50:50; 80:20; 70:30; and 50:50 v/v), and then with chloroform :methanol = 99:1 v/v. The antioxidant activity showed a decrease indicatedby an increase of IC 50 from 13.41 ppm for ethanolic extract to become121.81 ppm for the methylated compounds. The antibacterial activity alsoshowed a decrease after methylization due to the decrease of inhibitiondiameter of bacteria growth. Two isolates (isolate 1 and 2) obtained fromthe  purification  steps  were  characterized  using  UV-Visiblespectrophotometer and FT-IR. The results indicated the existence ofphenolic compunds showed by -OH stretching in 3,445 and 3,448 cm -1 ;and CH aromatic stretching in 3,010 and 3,030 cm -1 . Isolate 1 was higherin antioxidant and antibacterial activities than isolate 2.Key words : Uncaria gambir, methyl derivative, antibacterial, antioxidantactivities
PATOGENISITAS ISOLAT Phellinus noxius PADA JAMBU METE DAN BEBERAPA JENIS TANAMAN BERKAYU LAINNYA nFN Supriadi; E. M. Adhi; S. Rahayuningsih; M. Dahsyat
Jurnal Penelitian Tanaman Industri Vol 10, No 1 (2004): Maret 2004
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v10n1.2004.8-11

Abstract

Gejala busuk akar cokelat pada tanaman jambu mete di Sumbawa, khususnya Kecamatan Pekat, Dompu-NTB diasosiasikan dengan serangan Phellinus noxius. Secara ilmiah jamur ini belum dapat dibuktikan patogensitasnya. Penelitian ini betujuan menguraikan hasil penelitian tentang uji patogenisitas isolat P. noxius pada bibit jambu mete dan 6 jenis tanaman berkayu lainnya. Penelitian dilakukan pada tahun 2003 di laboratoium dan rumah kaca Balai Penelitian Tanaman Rempah dan Obat. Isolat P. noxius diperoleh dari tanaman jambu mete sakit Kecamatan Pekat, Dompu-NTB, kemudian diperbanyak pada medium campuran beras jagung (1:1) dalam botol selai (vol. 250 ml.). Biakan inokulum jamur berumur salu bulan diinokulasikan pada pangkal batang dai tujuh jenis tanaman berkayu, yaitu: jambu mete (Anacardium occidentale) jenis Balakrisnan, kayu manis (Cinnamomum casia dan C. burmanii), kopi (Cofea arabtca), jarak pagar (Jatropa curcas). kapok (Ceiba pentandra), dan singkong (Manihot utilissima) yang ditumbuhkan di dalam kantong plastik. Hasil penelitian menunjukkan bahwa enam dai tanaman yang diinokulasi dengan P. noxius menghasilkan gejala penyakit daun menguning dan layu, sama sepeti gejala penyakit di lapangan. Bibit yang diinokulasi mati dalam waktu 2-3 minggu sampai dengan 2 bulan setelah inokulasi. Satu-satunya jenis tanaman yang menunjukkan gejala berbeda dan tidak mati, adalah singkong yang menunjukkan gejala kcrdil. Tanaman jambu mete dan jarak pagar merupakan tanaman inang yang baru untuk P. noxius. karena tanaman lainnya sudah pemah dilaporkan sebelumnya.. Mengingat ganasnya serangan P. noxius pada bibit yang diinokulasi maka kcwaspadaan perlu ditingkatkan untuk mencegah tersebamya penyakit ini ke daerah pengembangan mete lainnya di NTB.Kata kunci: Anacardium occidentale, jambu mete, Phellinus noxius. patogenisitasABSTRACTPathogenicity of Phellinus noxius isolatedfrom diseased cashew and other woody plantsBrown root rot symptom on cashew in Sumbawa, especially in Pekat Distict, Dompu - West Nusa Tenggara is associated with the attack of Phellinus noxius. The pathogenicity of this fungus has not been proven scientiically. This experiment was aimed to analyse the result of pathogenicity test of P. noxius isolate on the seedlings of cashew and 6 other woody plants. This research was done in 2003 in the laboratory and glass house of the Indonesian Spice and Medicinal Crop Research Institute. The P. noxius isolate was obtained from the infected cashew in Pekat District, Dompu - West Nusa Tcnggara, then multiplied in the mixture of rice and com medium in the 250 ml jam bottle. The one month fungus culture was inoculated on the stem base of the seven woody plants, namely cashew (Anacardium occidentale) Balakhrisnan cullivar, cinnamon (Cinnamommum casia and C. burmanii), coffee (Coffea arabica), castor (Jatropa curcas), kapok (Ceiba pentandra) and cassava (Manihot uilissima) grown in plastic pots. The result of this expeiment indicated that the six kinds of plants inoculated with /' noxius showed disease symptoms, such as wilting and yellowing of the leaves and died in about 2-3 weeks up to 2 months ater inoculation. The only plant that was not died but showed different symptom was cassava, its growth was very stunted but not died. The two plants, i.e. cashew and castor were new host plants for P. noxius. Considering the viciousness of /'. noxius atack on the inoculated seedlings, therefore the awareness to prevent the spread of this disease to other cashew plantations in West Nusa Tenggara should be raised.Key words : Anacardium occidentale. cashew, Phellinus noxius. pathogenicity
VARIETAS HIBRIDA HARAPAN TEMBAKAU MADURA SUWARSO SUWARSO; ANIK HERWATI; FATKHUR ROCHMAN; SLAMET SLAMET; BAMBANG SUPRIYADI; YASIN B. E.
Jurnal Penelitian Tanaman Industri Vol 8, No 1 (2002): Maret, 2002
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v8n1.2002.27-31

Abstract

Four hybrids resulted from the first generation of the crossing of madura and oiental tobaccos and Prancak-95 aa a standard vaiety were tested at several locations from 1997 to 2000 randomized block design. The experiments aimed at obtaining promising hybid varieties of the madura tobacco. The yield potency, quality and stability of the hybrids were tested by using the method of yau and HAMBLIN (1994), which was based on lite highest average and the loweat standard deviation. Two promising hybrids, i.e. PIS and PXA had quality and crop indexes higher and had lower content of nicotine compared to that of Prancak 95, and they were more adaptive compared with Prancak-95. The quality indexes of PIS, PXA and Prancak-95 were respectively 79.30 ; 75.65 and 65.02.
PENYIMPANAN IN VITRO TANAMAN PURWOCENG (Pimpinella pruatjan Molk.) MELALUI APLIKASI PENGENCERAN MEDIA DAN PACLOBUTRAZOL I. ROOSTIKA; R. PURNAMANINGSIH; I. DARWATI
Jurnal Penelitian Tanaman Industri Vol 15, No 2 (2009): Juni 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v15n2.2009.84-90

Abstract

ABSTRAKPurwoceng (Pimpinella pruatjan Molk.) adalah tanaman obatlangka asli Indonesia yang dikategorikan hampir punah. Konservasi in situtidak dapat diandalkan karena rusaknya habitat alami (hutan konservasi),sedangkan konservasi ex situ di lapang menghadapi kendala karenapurwoceng sulit dibudidayakan di luar habitat aslinya. Dengan demikian,konservasi in vitro merupakan alternatif yang dapat diterapkan untukmenghindari kepunahan tanaman purwoceng. Tujuan penelitian untukmengetahui efek dari kombinasi perlakuan pengenceran media dankonsentrasi paclobutrazol terhadap pertumbuhan kultur purwoceng, dayaregenerasi dan stabilitas genetik pasca penyimpanan. Penelitian dilakukanpada tahun 2004 di Laboratorium Kultur Jaringan, Balai Penelitian danPengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Bogorselama 9 bulan. Bahan tanaman yang digunakan bersumber dari koleksitanaman purwoceng di Kebun Percobaan Gunung Putri, Balai PenelitianTanaman Rempah dan Obat. Kegiatan penelitian mencakup: (1)Perbanyakan tunas in vitro purwoceng sebagai sumber eksplan denganmenggunakan regenerasi, yaitu media DKW + BA 1 ppm + Thidiazuron0,2 ppm + arginin 100 ppm, (2) Penyimpanan in vitro tunas purwocengdalam media DKW (1, ½, dan ¼ dosis) + paclobutrazol (0, 1, 3, dan 5ppm), (3) Regenerasi kultur purwoceng pasca penyimpanan in vitro padamedia regenerasi, dan (4) Evaluasi karakter sitologi kultur yang telahdisimpan melalui penghitungan jumlah kloroplas sel penjaga stomata.Rancangan percobaan disusun secara faktorial dalam lingkungan acaklengkap dengan 6 ulangan. Hasil penelitian menunjukkan tidak adanyainteraksi yang nyata antara pengenceran media dan konsentrasipaclobutrazol. Periode simpan kultur tidak dapat diperpanjang lebih dari 4bulan karena paclobutrazol mempunyai pengaruh penghambatan pertum-buhan yang sangat kuat sehingga sebagian besar kultur purwoceng mati.Efek residu paclobutrazol masih tampak pada jangka waktu lebih dari 4bulan pada tahap pemulihan, ditandai dengan adanya penampilan roset.Pengamatan ciri sitologi melalui penghitungan jumlah kloroplas selpenjaga stomata menunjukkan bahwa penggunaan paclobutrazol tidakmenyebabkan perubahan tingkat ploidi. Disimpulkan bahwa paclobutrazoltidak sesuai digunakan untuk penyimpanan in vitro purwoceng karenamenyebabkan pertumbuhan yang abnormal (roset) sekalipun pada tahapregenerasi pasca penyimpanan. Selanjutnya disarankan untuk mengguna-kan regulator osmotik, yang mampu meningkatkan potensi osmotik dalammedia dan memperlambat penyerapan nutrisi sehingga masa simpankemungkinan dapat diperpanjang tanpa menyebabkan pertumbuhan yangabnormal pada tahap regenerasi pasca penyimpanan.Kata kunci : Pimpinella pruatjan Molk., penyimpanan in vitro, pengen-ceran media, dan paclobutrazolABSTRACTPurwoceng (Pimpinella pruatjan Molk.) is an Indonesian medicinalplant categorized as endangered plant. In situ conservation is quiteimpossible since conservation forest has been damaged whereas ex situconservation in the field is difficult because the plant needs specificagronomical condition. In vitro conservation is therefore the only choice tobe applied. The objectives of the study were to find out the effects ofcombined  treatment  between  media  dilution  and  paclobutrazolconcentration to the growth of pruatjan cultures, the genetic regenerationand stability after preservation. The research was conducted at the TissueCulture Laboratory, the Indonesian Center for Agricultural Biotechnologyand Genetic Resources Research and Development for 9 months. The plantmaterials were taken from Gunung Putri. The activities included: (1)Propagation of in vitro shoots as explants source in DKW media + 1 ppmBA + 0.2 ppm Thidiazuron + 100 ppm arginin, (2) Preservation of in vitroshoots of pruatjan on DKW (full, half, and quarter strength) +paclobutrazol (0, 1, 3, and 5 ppm), (3) Regeneration of the cultures after invitro preservation, and (4) Evaluation of cytological character of preservedcultures through chloroplast guard cells counting. The experiment wasarranged factorially in Completely Randomized Design with 6replications. The result revealed that there was no interaction betweenmedia dilution and paclobutrazol concentration. Preservation period couldnot be prolonged more than 4 months because this compound stronglyinhibited the growth so that almost none of them could survive longer. Theresidual effect of paclobutrazol was still appeared more than 4 months inregeneration phase assigned by rossette performances. Observation ofcytological character through chloroplast guard cells counting revealedthat paclobutrazol could not change ploidy level of preserved pruatjancultures. It was concluded that paclobutrazol is not suitable for in vitropreservation of pruatjan since it causes abnormal growth on regenerationstep after preservation. Thus, it was suggested to use osmotic regulatorwhich can increase osmoticum potential in media and decrease nutritionabsorption so that preservation period may be prolonged without abnormaleffect on regeneration step after preservation.Key words: Pimpinella pruatjan Molk., in vitro preservation, mediadilution, and paclobutrazol
POTENSI CENDAWAN Synnematium sp. SEBAGAI AGENS PENGENDALI Lawana sp. (FLATIDAE; HOMOPTERA) ELLYDA ABAS WIKARDI; GNR PURNAYASA; SISWANTO SISWANTO
Jurnal Penelitian Tanaman Industri Vol 7, No 3 (2001): September, 2001
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v7n3.2001.84-87

Abstract

Potency of Synnematium sp. as biocontrol agent of Lawana sp. (Flatidae; Homoptera)Research on potency of Synnematium sp. as biocontrol agent of Lawana sp. was carried out from May to June 2001 with a scries of pathogenicity tests in ield laboratory Narmada (Mataram) and Insect Laboratory Balittro, Bogor. The research consisted of two pats ie: (I) inoculation of the fungi on egg cluster of Lawana sp. of different ages, and (2) inoculation of the fungi with various application methods to the imagoes of Lawana sp.. Results showed that Synnematium sp. could infect 0-4 days old of eggs. Eggs more Uian 4 days old could be also infected, however 3-5% could still hatch to be nymphs. Application to parasitized eggs did not affect the parasitoid to hatch. Tests on the adults showed that 3 days ater inoculation with the fungi, adults of Lawana sp. became weak, while the motality occurred ater 5 days inoculation. Colony of Synnematium sp. on PDA media, primarily was white, then dark small balls sclcrotium, and formed synnemata on the top. The presence of sclerotia and synnemata seemed that the fungi is potential to be developed as microbial insecticide material ("micoinsectiside").
PENGARUH PEMUPUKAN Si CAIR TERHADAP PRODUKSI DAN RENDEMEN TEBU / Effect of Liquid Si Fertilizer on Yield and Commercial Can e Content of Sugarcane Djajadi Djajadi; Sulis Nur Hidayati; Roni Syaputra; Supriyadi Supriyadi
Jurnal Penelitian Tanaman Industri Vol 22, No 4 (2016): Desember, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n4.2016.176-181

Abstract

Sugarcane is proved to absorb more Si than any other nutrient from soil; therefore continuous cropping of the plant at the same soil would bring consequences of more Si and organic matter depletion. Silicon (Si) is considered a beneficial nutrient for sugarcane production. Field study was carried out in 2013-2014 at Kempleng village; Purwoasri sub district; Kediri district; East Java. The objective was to find out the effect of frequency and concentration of Si liquid fertilizer on Si and N absorptions, stem diameter and length, yield and commercial cane sugar (CCS) of sugarcane var PSBM 901. Split plot design with three replicates was employed to arrange the treatments. As main plots were frequency of spraying (one time, two times, three times, and four times with interval of 20 days started at 30 days after planting), while as sub plots were concentration of Si fertilizer (0, 15% Si, 30% Si). Results showed that interaction between frequency and concentration of Si affected on all parameters observed. Spraying of sugarcane four times with 30% Si gave highest value of cane yield, rendement, and sugar yield, i.e. 184.16 tons/ha and 8.36%, 15.37 ton/ha respectively.Keywords: sugarcane, yield, commercial cane sugar AbstrakTebu tergolong jenis tanaman akumulator unsur Si, sehingga penanaman secara terus menerus pada lahan yang sama akan berakibat semakin terkurasnya unsur Si dan menurunnya bahan organik dalam tanah. Unsur Si berperan penting terhadap pertumbuhan tebu terutama pada tanah-tanah tropis. Untuk mengetahui efektivitas pupuk Si cair pada tebu, telah dilakukan penelitian lapang pada tahun 2013-2014, yang berlokasi di Desa Kempleng, Kecamatan Purwoasri, Kabupaten Kediri, Jawa Timur. Tujuannya untuk mengetahui pengaruh frekuensi dan konsentrasi pemberian pupuk cair Si terhadap serapan Si dan N, pertumbuhan, produksi dan rendemen tebu varietas PSBM 901. Penelitian disusun dengan Rancangan Petak Terbagi dengan tiga ulangan. Sebagai petak utama adalah frekuensi penyemprotan (1 kali, 2 kali, 3 kali, 4 kali dengan interval 20 hari sekali sejak umur tebu 30 hari). Sebagai anak petak adalah konsentrasi pupuk Si (0, 15%, 30%). Hasil penelitian menunjukkan bahwa interaksi antara frekuensi penyemprotan dan konsentrasi pupuk Si berpengaruh terhadap peningkatan serapan Si dan N, diameter batang, panjang batang, produksi dan rendemen tebu. Penyemprotan pupuk Si pada tebu sebanyak 4 kali (umur 30, 50, 70 dan 90 hari) dengan konsentrasi 30% Si menghasilkan tebu dengan bobot dan rendemen tertinggi serta gula hablur, yaitu masing-masing sebesar 184,16 ton/ha dan 8,36% serta 15.37 ton/ha.Kata kunci: Tebu, Si, produksi, rendemen

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