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Buletin Plasma Nutfah
Published by Kementerian Pertanian
ISSN : 14104377     EISSN : 25491393     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Buletin Plasma Nutfah (BPN) is an open access scientific journal published by The Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), The Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. This peer-refereed journal covering the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation, of all kinds of genetic resources: plants, animals, fishes, insects and microbes. Manuscripts submitted to this journal are those that have never been published in other journals. This journal is published in one volume of two issues per year (June and December). We invite authors to submit the manuscripts to this journal in English or Indonesian. Detail information about the journal, including author guidelines and manuscript template, is available on the website (http://ejurnal.litbang.pertanian.go.id/index.php/bpn). The manuscript should be submitted electronically through our submission system. Buletin Plasma Nutfah (BPN) is an open access scientific journal published by Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. BPN Accredited by Ministry of Research, Technology and Higher Education of the Republic of Indonesia (No. 21/E/KPTP/2018) for period of 2016-2020. This peer-refereed journal covers the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation related to genetic resources: plants, animals, fishes, insects and microbes which has never been published in other Journal
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Articles 296 Documents
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Seleksi Galur Mutan Padi Fatmawati Tahan terhadap Penyakit Blas dan Evaluasi Karakter Agronomi di Rumah Kaca dan di Lahan Sawah Endang G. Lestari; Iswari S. Dewi; Rossa Yunita
Buletin Plasma Nutfah Vol 21, No 2 (2015): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v21n2.2015.p79-88

Abstract

Fatmawati, a rice variety released in 2003, is a new plant type (NPT) of rice that had different characteristic from existing high yielding rice varieties (HYV). The yield potential of this rice variety is 7.5 t/ha. Therefore its cultivated is expected to increase national rice production. However, the dissemination of this variety was hampered in several regions due to its susceptibility to blast disease and high percentage of empty grain caused by partially exserted and enclosed panicle. This research was conducted to evaluate 104 dihaploid mutant lines of Fatmawati for resistance to blast disease and good agronomic characters, especially for panicle exsertion rate. The first research was conducted in the endemic area of blast disease at Cikembar-Sukabumi, West Java. The blast disease resistance lines were then subsequently evaluated for their agronomic characters by planting in the green house and in the field. The results indicated that 40 dihaploid mutant lines were characterized as resistant and moderately resistant to blast (score 3–5). Agronomic characters evaluation in the green house and in the field resulted in 11 dihaploid mutant lines with well exerted panicle (0% of coverage of panicle by flagleaf sheath) and high grains per panicle (229–283 grains/panicle). Those lines were F99, F102, F116, F122, F130, F134, F138, F147, F149, F150, and F153. AbstrakFatmawati, varietas padi yang dilepas tahun 2003, adalah padi tipe baru (PTB) yang mempunyai karakteristik berbeda dari varietas padi unggul baru (VUB). Potensi hasil varietas padi ini adalah 7,5 t/ha. Penanaman varietas ini diharapkan akan meningkatkan produksi padi nasional. Namun, diseminasi varietas ini di beberapa tempat mengalami hambatan karena kerentanannya terhadap penyakit blas dan tingginya persentase gabah hampa akibat setengah tertutupnya atau tertutup semuanya malai oleh pelepah daun bendera. Penelitian ini dilakukan untuk mengevaluasi 104 galur mutan dihaploid turunan Fatmawati terhadap ketahanan blas dan karakter agronomi, khususnya laju keluarnya malai. Penelitian pertama dilakukan di daerah endemik penyakit blas di Cikembar- Sukabumi, Jawa Barat. Galur-galur yang tahan blas kemudian dievaluasi karakter agronominya dengan menanam di rumah kaca dan lapang. Hasil penelitian menunjukkan terdapat 40 galur mutan dihaploid dikategorikan sebagai tahan dan agak tahan penyakit blas (skor 3–5). Evaluasi karakter agronomi di rumah kaca dan lapang menghasilkan 11 galur mutan dihaploid yang malainya sempurna keluar (0% penutupan malai oleh pelepah daun bendera) dan jumlah gabah/malai tinggi (229–283 gabah/malai). Galur mutan dihaploid tersebut adalah galur F99, F102, F116, F122, F130, F134, F138, F147, F149, F150, dan F153.
Analisis Stabilitas Hasil Ubi 27 Genotipe Bengkuang (Pachyrhizus erosus L. Urban) di Jatinangor Jawa Barat Berdasarkan Model AMMI Sosiawan Nusifera; Agung Kurniawan
Buletin Plasma Nutfah Vol 14, No 1 (2008): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v14n1.2008.p19-25

Abstract

Research aimed at estimating yield stability of 27 yam bean (Pachyrhizus erosus L. Urban) genotypes in Jatinangor was conducted at dry and rain seasons at the experimental station of Faculty of Agriculture Unpad, Jatinangor. Field trials at dry season was started from February to August 2006 and at rain season started from November 2006 to May 2007. Field plot consisted of four sets arranged in Randomized Block Design with 27 genotypes collected from various Indonesia regions and its ancestor from Central and South America as treatment and replicated twice. The four field trial sets were differed based on season and reproductive pruning treatment and considered as four different environment. Character observed was tuber weight per plant (g). Data was analysed with AMMI (additive main effect and multiplicative interaction). Result indicated that B-23/EC040 was highest yielding genotype, but less stable. In contrast, B-33/J, B-26/NS, B-10/EC550, and B-94/ENT were moderate yielded but had higher level of stability. Environment IV (rain season; pruning) was good environment where genotypic variation seemed more consistent with B-23/EC040 as best genotype. Best genotype in discriminating environment (I) was B-55/CJ, and in environment II was B-80/ENT. Whereas in Less discriminating environment III, B-15/EC104 was the best genotypeAbstrakPenelitian yang bertujuan untuk mengetahui stabilitas hasil dari 27 genotipe bengkuang (Pachyrhizus erosus L. Urban) telah dilakukan pada musim kemarau dan musim hujan di Kebun Percobaan Fakultas Pertanian Unpad, Jatinangor. Percobaan pada musim kemarau berlangsung sejak Februari-Agustus 2006 dan percobaan pada musim hujan berlangsung sejak November 2006-Mei 2007. Percobaan terdiri atas empat set, masing-masing disusun dalam rancangan acak kelompok dengan 27 genotipe bengkuang yang dikoleksi dari berbagai wilayah Indonesia dan genotipe leluhurnya dari Amerika Tengah dan Selatan sebagai perlakuan dan diulang dua kali. Empat set percobaan tersebut dibedakan berdasarkan kombinasi musim dan perlakuan pemangkasan sink reproduktif, atau representasi dari empat lingkungan yang berbeda. Karakter yang diamati adalah bobot ubi per tanaman. Data dianalisis dengan model AMMI (additive main effect and multiplicative interaction). Hasil penelitian menunjukkan B-23/EC040 adalah genotipe berdaya hasil tertinggi, namun kurang stabil. Sebaliknya, B-33/J, B-26/NS, B-10/EC550, dan B-94/ENT adalah genotipe dengan hasil di atas rata-rata namun memiliki stabilitas yang lebih tinggi. Lingkungan IV (musim hujan dengan pemangkasan) adalah lingkungan baik di mana variasi genotipe terlihat lebih konsisten dengan B-23/EC040 sebagai genotipe terbaik. Genotipe terbaik pada lingkungan I adalah B-55/CJ, pada lingkungan II B-80/ENT, dan pada lingkungan III B-15/EC104.
Variasi Genetik Jeruk Keprok SoE (Citrus reticulata Blanco) Hasil Radiasi Sinar Gamma Menggunakan Penanda ISSR Farida Yulianti; C. Martasari; nFN Karsinah; Tangguh Hartanto
Buletin Plasma Nutfah Vol 16, No 2 (2010): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n2.2010.p134-139

Abstract

Genetic Variation of Keprok SoE Mandarin (Citrus reticulata Blanco) Resulted from Gamma Iradiation Based on ISSR Markers. SoE mandarin is one of the best mandarin from Indonesia which has been chosen as one of mandarin for import substitution. The citrus quality could be improved through breeding program, one of this program was mutation breeding using gamma rays irradiation. The research was aimed to obtain information of SoE mandarin genetic variation derived from gamma rays irradiation using ISSR marker. The research was conducted at Breeding and Tissue Culture Laboratory of Indonesian Citrus and Subtropical Research Institute (ICISFRI) from May to September 2008. Five ISSR primers were used to amplify DNA samples. Matrix data was counted and dendrogram of samples was established using UPGMA and SAHN methods. The resulst showed that 3 of the primers indicated polymorphism. About 22 locus were amplified from 3 primers, 9 (40.9%) locus showed polymorphism. The genetic similarity of SoE mandarin derived from gamma rays irradiation were 73-100%. AbstrakJeruk keprok SoE merupakan salah satu jeruk keprok unggulan Indonesia untuk mensubstitusi jeruk impor. Kualitas jeruk dapat ditingkatkan melalui program pemuliaan, salah satunya adalah melalui pemuliaan mutasi dengan menggunakan sinar gamma. Tujuan penelitian ini adalah untuk mendapatkan informasi tentang variasi genetik jeruk keprok SoE hasil radiasi sinar gamma menggunakan penanda ISSR. Penelitian dilaksanakan di Laboratorium Pemuliaan dan Kultur Jaringan, Balai Penelitian Tanaman Jeruk dan Buah Subtropika (Balitjestro) pada bulan Mei-September 2008. Lima penanda ISSR digunakan untuk mengamplifikasi sampel DNA. Pengelompokan tanaman di dalam dendrogram dihitung menurut UPGMA menggunakan metode SAHN. Hasil penelitian menunjukkan bahwa tiga penanda mengindikasikan adanya polimorfisme. Dari 22 lokus yang terbentuk dari tiga penanda, sembilan lokus (40,9%) menunjukkan polimorfisme. Tingkat kesamaan genetik jeruk keprok SoE hasil radiasi sinar gamma berkisar antara 73-100%.
In Vitro Medium-Term Conservation of Several Carnation Accessions through Nutrient Modification Kurniawan Budiarto; Budi Marwoto; Rudy Soehendi
Buletin Plasma Nutfah Vol 19, No 1 (2013): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n1.2013.p%p

Abstract

Modification of osmotic pressure of in vitro medium-term canbe applied to conserve carnation accessions. This successfulmethod inspired the use of nutrient modification technique tohave the same function as far as these concerned. The researchwas then, conducted to evaluate in vitro media for preservingseveral carnation accessions in low temperature conditions.The research was carried out at Indonesian Ornamental CropsResearch Institute (IOCRI) from January 2007 to March 2008.A randomized completely block design with 25 replicationswas used to accomplish the combination of two factors. Thefirst factor was six commercial carnation accessions, namelycv. Light Pink Candy, Aicardy, Torpedo, Malaga, President,and Rendezvouz. While the second factor dealt with fourformulations of conservation media i.e. ½ MS + DMSO 3% +4% sucrose, modified hyponex + 4% sucrose, modifiedhyponex (no sucrose) and ½ MS + 4% sucrose (control). Theresults showed that medium term in vitro conservation forcarnation were successfully conducted up to 10 months usingin ½ MS + DMSO 3% + 4% sucrose and modified hyponex +4% sucrose without any significant differences and decreasesin viability when transferred in to shoot induction media. In theabsent of sucrose, however, the plantlet survivals were only upto 8 months. No significant variation on viability and numberof survival plantlet was observed among the carnationaccessions in all media tested.
Sebaran dan Potensi Keruing (Dipterocarpus spp.) di Pulau Siberut, Sumatera Barat N. M. Heriyanto; M. Bismark
Buletin Plasma Nutfah Vol 20, No 2 (2014): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v20n2.2014.p85-92

Abstract

Distribution and Potency of Keruing (Dipterocarpus spp.) on the Island of Siberut, West Sumatra. Nur. M. Heriyanto and M. Bismark. Distribution and potency of Dipterocarpus spp. (keruing) were studied in Siberut Biosphere Reserve area, covering Sot Boyak and Bekemen forest village, Siberut National Park, West Sumatra. Six research plots of 50 m x 50 m (0.25 ha) were laid down. The results showed that Dipterocarpus spp. density at the tree level were 31 trees/ha at Sot Boyak and 38 trees/ha at Bekemen forest village with important value index of 63.83% and 77.54% respectively. The strongest association of other trees to Dipterocarpus spp. were Hopea dryobalanoides Miq., Baccaurea bracteata Muell. Arg. and Endospermum diadenum Miq. as reflected respectively by 0.69, 0.67 and 0.56 of Ochiai index. Dipterocarpus spp. were commonly located on the slopes and ridges. The best grouping was on 50-59% Slopes. Slope relationship with the population distribution of Dipterocarpus spp. showed by the equation of Y = 5.83 ln (x) + 3.44 with R2 = 0.90. Regeneration of Dipterocarpus spp. in natural forest is highly dependent on the recruitment population. Saplings recruitment population was 556 individuals/ha, and seedling was 3,434 individual/ha. AbstrakSebaran dan potensi Dipterocarpus spp. (keruing) diteliti di kawasan Cagar Biosfer Siberut yang meliputi hutan Desa Sot Boyak dan Bekemen, Taman Nasional Siberut, Sumatera Barat. Contoh plot penelitian dibuat dengan ukuran 50 m x 50 m (0,25 ha) dengan enam ulangan. Hasil penelitian menunjukkan, kerapatan Dipterocarpus spp. tingkat pohon di hutan Desa Sot Boyak 31 pohon/ha dan Bekemen 38 pohon/ha dengan indeks nilai penting masing-masing 63,8% dan 77,5%. Asosiasi terkuat dengan pohon lain terhadap keberadaan Dipterocarpus spp. adalah Hopea dryobalanoides Miq. indeks Ochiai 0,69, jenis Baccaurea bracteata Muell. Arg. (indeks Ochiai 0,67) dan Endospermum diadenum Miq. (indeks Ochiai 0,56). Habitat Dipterocarpus spp. berada di lereng dan punggung bukit, pengelompokannya terbaik pada kelerengan 50– 59%. Hubungan kemiringan lahan dengan sebaran populasi Dipterocarpus spp. mengikuti persamaan Y = 5,83l n(x) + 3,44 dengan R2 = 0,90. Regenerasi Dipterocarpus spp. di hutan alam sangat bergantung pada populasi anakan. Populasi anakan untuk tingkat belta sebesar di 556 individu/ha, tingkat semai 3.434 individu/ha.
Seleksi Galur Kentang dari Progeni Hasil Persilangan nFN Kusmana; Eri Sofiari
Buletin Plasma Nutfah Vol 13, No 2 (2007)
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v13n2.2007.p56-61

Abstract

Selection of progenies and lines of potato. Selection was performed at Indonesian Vegetable Research Institute (IVEGRI), Lembang (1250 asl) in 2006. Seven F1 progenies resulted from previous crossing were grown with population ranging from 50 to 200 seedlings. From the tuber yield had been selected 183 accessions. Out of these selected accessions, 173 accessions were planted with population ranging from 5 to 30 tubers. There were 55 lines showed promising as selected based on tuber yield that more than 300 g/plant, shallow to medium depth of tuber eyes, medium to large tuber size and good taste. There were four lines showed good quality for potato chips. AbstrakSeleksi dilakukan di Kebun Percobaan Balai Penelitian Tanaman Sayuran, Lembang (1250 m dpl) pada tahun 2006. Tujuh progeni F1 hasil persilangan ditanam dengan populasi 50-200 tanaman. Hasil seleksi pertama terpilih 183 aksesi, kemudian yang berhasil ditanam kembali sebanyak 173 aksesi. Tiap aksesi ditanam 5-30 umbi. Hasil seleksi kedua terpilih 55 galur dengan hasil lebih tinggi dari 300 g/tanaman, mata umbi dangkal dan medium, ukuran umbi relatif besar, dan rasa enak. Empat galur di antaranya sangat cocok untuk dijadikan bahan baku kripik kentang.
Pangasiid Catfishes of Indonesia Rudhy Gustiano
Buletin Plasma Nutfah Vol 15, No 2 (2009): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v15n2.2009.p91-100

Abstract

Pangasiidae are economically important riverine catfishes generally occurring in freshwater from the Indian subcontinent to the Indonesian Archipelago. Morphologically, they are recognized by a laterally compressed body, two pairs of barbels, a short dorsal fin with two spines, a well developed adipose fin, a long anal fin, and a strong pectoral spine. The systematics of this family are still poorly known. Consequently, the lack of this basic information is a great barrier in understanding the biology and hence the study of the aquaculture potential of species, the improvement of their seed production and growth performance. Objectives of the study are to present all species and genera of pangasiid catfishes of Indonesia including their distribution and detailed identification. AbstrakKelompok ikan pangasius atau pangasiidae merupakan jenis penting ikan “catfish” (bersungut) yang hidup di air tawar dan tersebar dari daratan India hingga kepulauan Indonesia. Secara umum ikan ini memiliki bentuk tubuh memanjang dan ramping, dua pasang sungut, sirip punggung yang relatif pendek dengan dua duri keras, sirip lunak tambahan yang berkembang sempurna sebagai pengontrol renang, dan tulang sirip dada yang kuat. Secara sistematik, kelompok ikan pangasius masih belum dikenal secara baik. Oleh karena itu, kurangnya informasi dasar yang dimiliki pada kelompok ikan ini merupakan hambatan utama untuk memahami sifat-sifat biologinya, pengembangan budidayanya, produksi benih, dan perbaikan keragaannya. Tujuan dari studi yang dilakukan adalah untuk menyajikan semua spesies dan genus ikan pangasius yang ada di Indonesia mencakup distribusi, sebaran, dan kunci identifikasinya secara detail.
Agronomics Characteristics and Its Correlation of New Plant Type Promising Rice Lines Angelita P. Lestari; Buang Abdullah; Ahmad Junaedi; Hajrial Aswidinnoor
Buletin Plasma Nutfah Vol 17, No 2 (2011): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v17n2.2011.p96-103

Abstract

Karakteristik Agronomi dan Korelasinya pada Galurgalur Harapan Padi Tipe Baru. Dalam program pemuliaan tanaman padi diperlukan pengetahuan tentang karakter dan korelasinya dengan hasil gabah. Penelitian dilakukan untuk mempelajari karakter tanaman dan korelasinya dari 35 galur harapan PTB dengan Ciherang dan Sintanur sebagai varietas pembanding, ditanam di dua lokasi, Bogor dan Pusakanagara, pada MT 2009 (MK dan MH). Rancangan yang digunakan adalah acak kelompok dengan tiga ulangan. Bibit umur 21 hari ditanam satu bibit per lubang, jarak tanam 20 cm x 20 cm, pada petak berukuran 2 x 5 m2 per baris. Karakter tanaman bervariasi antargalur dan beberapa di antaranya berbeda nyata dengan varietas pembanding Ciherang dan Sintanur, kecuali untuk hasil gabah. B11742-RS*2-3-MR-34-1-2-1 merupakan galur dengan postur tanaman terendah, hasil gabah rendah, dan umur terpendek. Sebagian besar galur mempunyai karakter padi tipe baru (PTB). Hasil gabah berkorelasi positif dengan semua karakter, tetapi hanya berkorelasi nyata dan positif dengan tinggi tanaman, berkisar antara 91,4-120,7 cm. AbstrakIn a plant breeding program, knowledge of the character and interrelationships among yield and yield contributing characters are necessary. This study was carried out to identify the plant character and its correlation between yield-correlated traits of 35 NPT rice promising lines with Ciherang and Sintanur as check varieties, planted in two locations, Bogor and Pusakanagara and two seasons (2009 dry and wet season, DS-WS). Those lines were planted in Randomized Complete Block Design (RCBD) arrangement, in three replications. The 21-days-old seedling planted one seed per hole, spacing 20 cm x 20 cm, with a plot size of 2 x 5 m2 per line. Plant characters varied and were significantly different from Ciherang and Sintanur as check varieties except for grain yield. B11742- RS*2-3-MR-34-1-2-1 was the line that had the lowest plant height, grain yield, and the shortest growth duration. Most of the lines had character as new plant type variety. Grain yield positively correlated with all characters, but only significantly and positively correlated with plant height at range from 91.4- 120.7 cm.
Kemampuan Berbunga, Tingkat Keguguran Bunga, dan Potensi Hasil Beberapa Varietas Kedelai Suyamto Suyamto; Musalamah Musalamah
Buletin Plasma Nutfah Vol 16, No 1 (2010): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n1.2010.p38-43

Abstract

Field experiments toidentify the flowering ability, flowering duration, percentageof flower abortion, and yield potential of several soybeanvarieties were conducted at two environments at Kendalpayak-Malang Research Station in April-July 2006 (dry season I) andGenteng-Banyuwangi Research Station (dry season II) in July-October 2006. A total of 20 soybean varieties were evaluatedby using randomized complete-blocks design with threereplications. The plot size was 0.8 m x 4.5 m with plantspacing 40 cm x 15 cm. The rate of fertilizers applied was 50kg urea, 100 kg SP36, and 75 kg KCl per ha. Plant control wasconducted intensively as recommended. Several charactersobserved were time to flower, flowering duration, number offlower, number of pod, number of filled pod, number of emptypod, seed yield/plant, and 100 seed weight. Combined analysisof variance show that all characters oberved were significantlyaffected by varieties. Flower abortion of soybean varietiesrange from a low of 6.6% for Lawit to a high of 39.1% forKawi. Lawit variety was used as high yielding potential parentwhich has lowest percentage of flower abortion (6.6%) andhighest percentage of flower to pod (93.4%).
Evaluasi Pertumbuhan Enam Varietas Mangga Merah Komersial yang Disambung dengan Teknik Top Working pada Dua Varietas Batang Antara Rebin Rebin; Lukitariati Sadwiyanti; Djoko Sudarso; Karsinah Karsinah
Buletin Plasma Nutfah Vol 20, No 1 (2014): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v20n1.2014.p1-10

Abstract

Evaluation of Growth of Six Commercial Red Mango Varieties Grafted by Top Working Technique on Two Interstock Varieties. Rebin, Lukitariati Sadwiyanti, Djoko Sudarso, and Karsinah. The objective of the research was to evaluate the growth and leaf anthocyanin content of six commercial red mango varieties that grafted by top working technique on two interstock varietties. The extensive development of commercial red mango as new superior variety in production center, especially in Java, faces limited land constraint. Meanwhile, the existing mango plants in growers is dominated by Arumanis and Durih varieties that are now old and less productive. In addition there are many local mango plant that are not good quality in their growth. In the condition mentioned, replacing less productive mango variety with commercial red mango through top working technology is a wise solution alternative. Research was conducted at Cukurgondang Experimental Field 50 m asl, Pasuruan, East Java from May until December 2009. The research was arranged in Split Plot Design where the main plot were two interstock varieties i.e.: Arumanis-143 and Durih, meanwhile the sub plot were six commercial red mango scions i.e.: Ken Layung, Marifta-01, Garifta Orange, Garifta Merah; Garifta Kuning and Garifta Gading. Each tratment were repeated three times. The results indicated that there were five varieties of red mango varieties compatible with Arumanis-143 interstock i.e.: Marifta-01, Garifta Orange, Garifta Merah, Garifta Kuning, and Garifta Gading. While Durih interstock was suitable for Ken Layung, Garifta Orange and Garifta Merah. Interstocks were not influence on leaf color of scion (both young and mature leaves). Anthocyanin content of Garifta Orange, Garifta Merah, and Garifta Gading leaves on Arumanis-143 interstock and on Durih interstock were higher than their mother plant respectively. The effect of Arumanis-143 was beter than Durih interstock on both vegetative character and leaf antocianin content of scion. AbstrakTujuan penelitian ini ialah mengevaluasi pertumbuhan dan kadar antosianin daun enam varietas mangga merah komersial yang disambung melalui teknik top working pada dua varietas batang antara. Pengembangan mangga merah komersial sebagai varietas unggul baru secara ekstensifikasi di sentra produksi, terutama di Jawa, mengalami kendala terbatasnya lahan. Sementara itu, pertanaman mangga yang ada di petani didominasi oleh varietas Arumanis dan Durih yang tanamannya sudah tua dan kurang produktif. Di samping itu banyak juga tumbuh mangga lokal yang kurang berkualitas. Dalam kondisi yang demikian, penggantian varietas mangga yang kurang produktif dengan mangga merah komersial melalui teknologi top working merupakan sebuah alternatif solusi yang bijaksana. Penelitian dilaksanakan di KP. Cukurgondang (Pasuruan) 50 m dpl, mulai Mei-Desember 2009. Perlakuan disusun dalam Rancangan Petak Terpisah (Split Plot Design) dengan Petak Utama berupa dua varietas batang antara, yaitu Arumanis-143 dan Durih. Sedangkan Anak Petak berupa enam varietas batang atas mangga merah komersial, yaitu Ken Layung, Marifta-01, Garifta Orange, Garifta Merah, Garifta Kuning, dan Garifta Gading. Setiap perlakuan diulang sebanyak tiga kali. Hasil penelitian menunjukkan bahwa lima varietas batang atas yaitu Marifta-01, Garifta Orange, Garifta Merah, Garifta Kuning, dan Garifta Gading kompatibel pada batang antara Arumanis-143. Sementara itu, batang antara Durih sesuai untuk batang atas Ken Layung, Garifta Orange, dan Garifta Merah. Batang antara tidak berpengaruh terhadap warna daun (baik daun muda maupun daun tua). Kadar antosianin daun pada varietas Garifta Orange, Garifta Merah, dan Garifta Gading yang di top working pada batang antara Arumanis-143 dan Durih lebih tinggi dibanding dengan kadar antosanin daun dari pohon induknya. Pengaruh batang antara Arumanis 143 lebih baik dibanding batang antara Durih terhadap sifat vegetatif maupun kandungan antosianin daun batang atas.

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