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Jurnal Tanaman Industri dan Penyegar
Published by Kementerian Pertanian
ISSN : 23561297     EISSN : 25287222     DOI : -
Jurnal Tanaman Industri dan Penyegar (JTIDP) published by Indonesian Center for Estate Crops Research and Development is a peer-reviewed and open access journal that publishes significant and important research from area of agricultural science on industrial and beverage crops.
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Articles 407 Documents
Potensi Trichoderma spp. dalam Menekan Perkembangan Penyakit Busuk Pucuk Vanili di Pembibitan Taufiq, Efi
Jurnal Tanaman Industri dan Penyegar Vol 3, No 1 (2012): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v3n1.2012.p49-56

Abstract

Penyakit busuk pucuk vanili (BPV) yang disebabkan oleh Phytophthora capsici merupakan kendala pada pembibitan dan pertanaman vanili. Pengendalian penyakit umumnya menggunakan fungisida sintetik yang harganya mahal dan menyebabkan pencemaran lingkungan. Pengendalian penyakit dengan agens hayati sudah dikembangkan dan berhasil mengatasi penyakit busuk pangkal batang pada tanaman vanili.  Penelitian ini bertujuan untuk mendapatkan isolat Trichoderma dari tanah, rizosfer dan jaringan tanaman vanili sebagai agens hayati terhadap P. capsici secara in vitro dan in vivo (pembibitan). Penelitian dilakukan di Laboratorium Mikologi Departemen Proteksi Tanaman IPB, Laboratorium Penyakit Balai Penelitian Tanaman Rempah dan Obat di Bogor, dan Rumah Kaca Balai Penelitian Tanaman Industri dan Penyegar di Sukabumi. Trichoderma spp. diisolasi dari risosfir dan jaringan tanaman vanili dari Serang, Sukabumi, dan Batu. Pengujian antagonisme in vitro dilakukan pada 17 isolat Trichoderma spp. menggunakan metode dual culture dan metode kertas cakram, sedangkan pengujian  in vivo dilakukan pada 6 isolat Trichoderma spp. menggunakan media jagung dan metode penyemprotan suspensi konidia agens hayati.  Peubah yang diamati adalah kejadian dan tingkat keparahan penyakit busuk pucuk pada vanili.  Penelitian menggunakan rancangan acak kelompok dengan 3 ulangan.  Hasil penelitian diperoleh 114 isolat jamur; 97 dari rizosfir, 11 dari tajuk dan 6 dari akar (endofit).  Hasil pengujian patogenisitas menunjukkan bahwa 63 isolat bersifat patogenik  pada tanaman vanili (umumnya  genus Fusarium) dan 51 isolat  tidak patogenik (umumnya Trichoderma).  Daya hambat in vitro isolat Trichoderma spp. terhadap P. capsici berkisar antara 44,5-73,5%, sedangkan dengan metode cakram daya hambatnya  6,3-75%. Keefektifan 6 isolat Trichoderma spp. menekan perkembangan penyakit busuk pucuk pada bibit vanili berkisar antara 66,67-68,00%. Hasil penelitian mengindikasikan bahwa ada beberapa isolat Trichoderma spp yang berpotensi sebagai agens hayati untuk menekan perkembangan penyakit busuk pucuk vanili yang disebabkan oleh P. capsici.   Potential of Trichoderma spp. To suppress development of the shoot rot disease of vanilla in nurseriesABTRACT Shoot rot disease of vanilla (BPV) is caused by Phytophthora capsici is a constraint on the seedling and planting vanilla. Disease control generally use synthetic fungicides are expensive and cause environmental pollution. Disease control with biological agents has been developed and successfully overcome the base of the stem rot disease in vanilla plants. This study aims to obtain isolates of Trichoderma from soil, rhizosphere and plant tissue vanilla as a biological agent against P. capsici in vitro and in vivo (seedling). Mycological Research conducted at the Laboratory of Plant Protection Department of IPB, Laboratory Center for Disease Spices and Medicinal Plants Research in Bogor, and Greenhouse Crops Research Institute for Industry and freshening in Sukabumi. Trichoderma spp. isolated from plant tissue,  rizosphere and vanilla from Serang, Sukabumi, and Batu. Invitro antagonism test performed on 17 isolates of Trichoderma spp. using the dual culture method and the method of the paper disc, whereas in vivo tests carried out on six isolates of Trichoderma spp. using corn media and methods of spraying conidia suspensions of biological agents. Observed variable is the incidence and severity of shoot rot disease in vanilla. Research using randomized block design with three replications. The results obtained 114 isolates of the fungus; 97 of the rizosphere, 11 of the canopy and 6 of the root (Endophytic). The results of pathogenicity tests showed that 63 isolates are pathogenic on plants vanilla (generally  Fusarium) and 51 isolates were not pathogenic (generally  Trichoderma). The inhibition of in vitro isolates of Trichoderma spp. against P. capsici ranged from 44.5 to 73.5%, while the disc method hambatnya power from 6.3 to 75%. The effectiveness of six isolates of Trichoderma spp. suppress the development of bud rot disease in vanilla seeds ranged from 66.67 to 68.00%. The results indicate that some isolates of Trichoderma spp as potential biological agents to suppress the development of shoot rot disease of vanilla caused by P. capsici.
Keragaman Genetik Beberapa Genotipe Teh Berdasarkan Penanda RAPD (Random Amplified Polymorphic DNA) Martono, Budi; Udarno, Laba
Jurnal Tanaman Industri dan Penyegar Vol 1, No 1 (2014): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v1n1.2014.p1-6

Abstract

Informasi keragaman genetik dan ketersediaan plasma nutfah teh (Camellia sinensis) diperlukan dalam perakitan varietas unggul. Keragaman genetik berdasarkan penanda DNA dapat memberikan hasil yang lebih konsisten karena tidak dipengaruhi lingkungan. Dalam penelitian ini sebanyak 9 genotipe teh dianalisis keragamannya menggunakan enam penanda RAPD (OPA 03, OPA 05, OPB 04, OPB 06, OPC 06, dan OPD 08). Penelitian dilakukan mulai bulan Maret sampai Mei 2013 di Laboratorium Terpadu Biotrop Bogor. Perhitungan koefisien kesamaan genetik dan analisis gerombol dilakukan dengan menggunakan perangkat lunak NTSYSpc versi 2.02. Sebanyak 54 lokus penanda RAPD berhasil diamplifikasi menggunakan enam primer dan 47 lokus di antaranya memiliki alel yang polimorfik (87,04%). Hasil analisis gerombol berdasarkan kesamaan genetiknya mengelompokkan 9 genotipe ke dalam enam kelompok. Empat kelompok (I, II, IV, V) masing-masing terdiri atas satu genotipe, sementara dua kelompok yang lain yaitu kelompok III dan VI masing-masing beranggotakan tiga dan dua genotipe.Kata Kunci: Camellia sinensis, diversitas genetik, penanda RAPDThe availability of diverse tea (Camellia sinensis) germplasms as well as the information about their genetic diversity is required for plant breeding program. Genetic diversity analysis based on DNA marker is known to be more effective since the markers provide more consistent results. In this study, nine tea genotypes were evaluated for their genetic diversity using six Random Amplified Polymorphic DNA (RAPD) markers (OPA 03, OPA 05, OPB 04, OPB 06, OPC 06, and OPD 08). The study was conducted from March to May 2013 in the Integrated Laboratory of Biotrop Bogor. The estimation of genetic similarity and the cluster analysis were done using NTSYSpc version 2.02. Of the six RAPD markers used in this study, a total of 54 RAPD marker loci have been successfully amplified. In which, 47 loci (87.04%) were polymorphic and subsequently used for the evaluation of tea genotypes. The results of cluster analysis showed that those tea genotypes were clustered into six groups. Each of four groups (I, II, IV, V) consisted of only one genotype. Meanwhile, the other two groups (III and VI) had three and two genotypes, respectively.
Analisis Faktor Penentu Adopsi Benih Unggul Karet Sudjarmoko, Bedy; Listyati, Dewi; Hasibuan, Abdul Muis
Jurnal Tanaman Industri dan Penyegar Vol 4, No 2 (2013): Buletin Riset Tanaman Rempah dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v4n2.2013.p117-128

Abstract

Adopsi benih unggul karet untuk perluasan dan peremajaan tanaman pada perkebunan rakyat masih sangat rendah. Padahal benih unggul menjadi salah satu faktor penting dalam peningkatan produktivitas dan kualitas produk, serta dalam efisiensi produksi. Penelitian ini dilakukan untuk mengidentifikasi faktor-faktor yang mempengaruhi adopsi benih unggul karet, khususnya pada perkebunan rakyat dalam rangka mendukung kebijakan pengembangan karet nasional. Penelitian dilaksanakan di Kabupaten Sarolangko, Jambi pada Januari-Desember 2012. Data yang digunakan adalah data primer dan sekunder. Analisis data menggunakan model persamaan struktural/Structural Equation Modeling (SEM). Hasil penelitian menunjukkan bahwa adopsi benih unggul karet dipengaruhi secara langsung oleh harapan petani (Product Performance Expectancy/PPE), Social and Technical Influence (STI), dan harga benih unggul karet. Untuk meningkatkan adopsi benih unggul oleh petani karet, diperlukan pengembangan atribut-atribut benih unggul, terutama umur panen dan kualitas benih. Percepatan proses adopsi benih unggul karet juga dapat dilakukan melalui kegiatan diseminasi teknologi, penyebaran buku-buku panduan, petunjuk teknis, petunjuk pelaksanaan, leaflet dan poster, serta teknik advokasi melalui kelompok tani.Kata Kunci: Karet, benih unggul, adopsi, SEMThe adoption of rubber superior seeds by farmers for replanting and new planting is still very low. Whereas, the superior seeds to be one important factor in increasing the productivity and quality of products, as well as in production efficiency. The objective of study was to determine factors affecting the adoption of rubber superior seeds, especially to support national policy in developing of smallholder rubber development. The study was conducted in Sarolangko District, Jambi on January-December 2012. The primary and secondary data was used in this study, and then analyzed with Structural Equation Modeling (SEM). The results showed that the adoption of rubber superior seeds was directly affected by expectations farmers (Product Performance Expectancy/PPE), Social and Technical Influence/STI, and price of rubber superior seeds. To increase the adoption of rubber superior seeds it need the development of their attributes, especially in harvest old and quality of seeds. Also, to accelerate the adoption of rubber superior seeds can be done by the extentions in using of rubber superior seeds, and by dissemination of technologies through the handbooks, guidelines/technical manual/leaflet/poster/brochure, as well as the advocacy technic through the farmer groups.
EVALUASI KETAHANAN SEPULUH AKSESI JAMBU METE TERHADAP PENYAKIT BUSUK AKAR FUSARIUM Supriadi, Handi; Taufik, Efi; Harni, Rita
Jurnal Tanaman Industri dan Penyegar Vol 2, No 2 (2011): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Abstract

Cashew is a commodity that has an important role in improving the welfare of farmers, especially in areas with dry climates and poor nutrients. One obstacle in the development of cashew nut is the presence of Fusarium  attacks that can shut down the plant in the nursery. Research evaluation of resistancies of ten accessions of cashew against Fusarium root rot disease, which aimed to get the accessions that resistant to Fusarium root rotdisease has been conducted in the Laboratory and Greenhouse of Indonesian Spices and Industrial Crops Rresearch Institute (ISICRI) January to December 2009. Research using completely randomized design with 10 treatments and three replications. Treatments were the type of accsession namely Arsyad Labone, Sri Lanka,JT-21, Laode Head, Laode Gani, Sleman Red, Nigeria, Laode Kase, JN 26, NDR-31. The result obtained one cashew accessions highly resistant namely JN-26, and two accessions that resistant that is Laode Gani, and Sleman Red with root rot disease incidence of each 0; 6.67, and 9.52% at the age of two months after inoculation.
EVALUASI PRODUKSI DAN FISIKO-KIMIA MINYAK CENGKEH ZANZIBAR GORONTALO Supriadi, Handi; Syafaruddin, Syafaruddin; Bermawie, Nurliani; Hadad EA, Mochamad
Jurnal Tanaman Industri dan Penyegar Vol 3, No 3 (2012): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v3n3.2012.p269-276

Abstract

Cengkeh Zanzibar Gorontalo telah lama dijadikan sebagai sumber benih oleh petani cengkeh di Provinsi Gorontalo karena cengkeh tersebut memiliki keunggulan yaitu berproduksi tinggi dan aroma bunganya disukai oleh banyak pabrik rokok  kretek. Penelitian ini bertujuan mengevaluasi produksi, fisiko-kimia minyak dan karakteristik morfologi 17 pohon induk cengkeh Zanzibar Gorontalo yang sudah digunakan sebagai sumber benih oleh petani. Kegiatan dilaksanakan di Desa Taludaa, Kabupaten Bone Bolango, Gorontalo, mulai Januari sampai September 2012, dengan metode survei. Hasil penelitian menunjukkan bahwa cengkeh Zanzibar Gorontalo memiliki produksi bunga basah lebih tinggi dibandingkan cengkeh Afo dan komposit Zanzibar Karo. Pohon induk tersebut memiliki mutu bunga cengkeh yang baik dengan kadar minyak atsiri 19,94-23,00% dan kadar eugenol total 87,43-93,00%. Ukuran bunga lebih besar dengan aroma bunga khas, serta seragam dalam penampilan karakter vegetatif dan generatif tanaman.EVALUATION OF GORONTALO ZANZIBAR CLOVE PRODUCTION AND ITS PHYSICO-CHEMICAL ESSENTIAL OILABSTRACT The Clove of Zanzibar Gorontalo has been used as a source of seeds by farmers in Gorontalo province due to high production and its scents preferred by many cigarette industries. A research was carried out to evaluate production and physico-chemical properties of essential oil of Zanzibar clove trees grown. The study (in form of surveys) was focused on the morphological characters (vegetative and generative) of 17 mother trees of Zanzibar clove from January to September 2012 at Taludaa village, Bone Bolango district, Gorontalo. The results showed that the fresh yield of Gorontalo Zanzibar clove was 133,46 kg/tree/year being much higher than those of Afo and Zanzibar Karo composite varieties, 103.0 and 47.0 kg/tree/year respectively. In addition to high in yields, the clove mother trees have good quality in essential oil (19,94-23,00%) and total of eugenol content (87,43-93,00%), larger in flower sizes, distinctive in floral aroma, and homogeny in performance of vegetative and generative characters.
Analisis Perdagangan Kopi Indonesia di Pasar Internasional Meiri, Anggi; Nurmalina, Rita; Rifin, Amzul
Jurnal Tanaman Industri dan Penyegar Vol 4, No 1 (2013): Buletin Riset Tanaman Rempah dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v4n1.2013.p39-46

Abstract

Indonesia merupakan salah satu negara produsen dan eksportir kopi terbesar di dunia. Akan tetapi, pertumbuhan volume ekspor kopi di Indonesia lebih rendah dari pertumbuhan produksinya. Rendahnya ekspor kopi Indonesia disebabkan oleh banyak faktor yang mempengaruhi perdagangan kopi Indonesia di pasar internasional. Tujuan dari penelitian ini adalah menganalisis faktor-faktor yang mempengaruhi perdagangan kopi Indonesia di pasar internasional dan menerangkan potensi perdagangan kopi Indonesia di negara tujuan. Metode analisis yang digunakan adalah analisis data panel dengan gravity model dan analisis potensi perdagangan. Hasil penelitian menunjukkan bahwa peubah yang berpengaruh signifikan terhadap ekspor kopi Indonesia adalah GDP riil/kapita Indonesia, GDP riil/kapita negara tujuan, jarak ekonomi antara Indonesia dengan negara tujuan, dan keanggotaan WTO. Sementara itu, Indonesia memiliki potensi untuk melakukan ekspansi perdagangan kopi ke negara-negara tujuan di masa yang akan datang karena perdagangan kopi Indonesia di negara tujuan masih under trade. Implikasi kebijakan adalah Indonesia harus meningkatkan pangsa pasar dengan memprioritaskan untuk mengekspor kopi ke Mesir dan Aljazair karena kedua negara tersebut memiliki pertumbuhan GDP riil/kapita yang tinggi dan perdagangan kopi Indonesia di Mesir dan Aljazair masih under trade.Kata Kunci: Kopi, ekspor, gravity modelIndonesia is one of the largest coffee producer and exporter in the world. However, the growth of Indonesia’s coffee export volume is lower than that of its production. Indonesia’s coffee export is low due to many factors affecting the Indonesia’s coffee trade in international market. The purposes of this study are to explain the factors that influence Indonesia’s coffee trade in international market and the trade potential of Indonesian coffee in destination countries. The analysis methods used are analysis of panel data with gravity model and trade potential analysis. The results showed that variables that significantly influence Indonesia’s coffee exports are real GDP/capita of Indonesia, real GDP/capita of destination countries, economic distance between Indonesia and destination countries, and WTO membership. Meanwhile, Indonesia has potency to expand coffee trade to the destination countries in the future. Therefore, Indonesia should increase the market share by prioritizing of exported coffee to Egypt and Algeria having high in real GDP/capita growth rate.
Potensi Rizobakteria dan Fungisida Nabati Untuk Pengendalian Penyakit Jamur Akar Putih Tanaman Karet Nasrun, Nasrun; Nurmansyah, Nurmansyah
Jurnal Tanaman Industri dan Penyegar Vol 2, No 2 (2015): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v2n2.2015.p61-68

Abstract

The white root fungus (WRF) disease caused by Rigidoporus microporus is the main disease that reduce rubber production. The use of biological agents, such as rhizobacteria and botanical fungicide is expected to control WRF disease. The research aimed to determine the potential of rhizobacteria and botanical fungicide with the active compound of citronellal, geraniol, eugenol, and catechin to suppress the growth of R. microporus and control WRF disease. The in vitro studies were conducted at the laboratory of KP. Laing, Solok, whereas in planta tests at rubber plantations in Sijunjung, West Sumatra from April to November 2012. The in planta tests used the factorial designs, which were arranged in a randomized complete block design with 2 factorials and 3 replications. The first factor was 4 treatments, using Bacillus sp. Bc94, Pseudomonas fluorescens Pf55, a combination of Bacillus sp. Bc94 and P. fluorescens Pf55, and without rhizobacteria. The second factor was 3 treatments, using biofungicide formula: formula F1, formula F2, and without botanical fungicide. The parameters assessed were inhibition of R. microporus growth and  development of WRF disease. The results showed that Bacillus sp. Bc94, P. fluorescence Pf55 and the combination of citronellal, geraniol, eugenol, and catechin had the highest inhibitions on R. microporus growth: 89.54%, 90.49%, and 81.39%, respectively. The combinations of Bacillus sp. Bc94 and P. fluorescens PF55 with botanical fungicide formulas (F1 and F2) significantly inhibited the WRF disease intensity up to 80.95%–82.91%.
ANALISIS MANAJEMEN RANTAI PASOK BENIH JAMBU METE (STUDI KASUS DI KABUPATEN FLORES TIMUR) Hasibuan, Abdul Muis; Wahyudi, Agus
Jurnal Tanaman Industri dan Penyegar Vol 2, No 2 (2011): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Abstract

East Flores is sourced reference of superio cashew seeds, so that this region is potentially a superior seed producer of cashew for development program in the future. This study aimed to analyze supply chain management (SCM) and development strategy of cashew superior seed. The research was conducted in East Flores, East Nusa Tenggara Province in June - August 2010. The data used are primary and secondary data. Analysis used model of supply chain management. The results showed that the demand for cashew seed is dependent on the procurement project undertaken by the government. The time often not coincide with the harvest of cashew seeds. Supply chain management applications has been running optimal although more are caused by government intervention in terms of distribution of seeds, so that the optimization process of SCM has not been produced from a process of good business activity. Strategies that can be achieved are: (i) government needs to encourage the process of seed certification by an official agency, (ii) developing the seed garden in an amount sufficient to consider the projected seed demand in the future, either in the form of seeds and scion, (iii) the farmer / farmer group, the owner of seed source should be able to apply the seed stock management so that demand outside the crop seed remain to be fulfilled, (iv) the breeder needs to make improvements in a poly bag seed distribution methods, (v) The Government needs to introduce grafting technology, particularly to the breeder and farmer groups, (vi) The Government needs to do the planning and mapping needs and promoting improved s eed varieties MPF 1. 
Keragaman 17 Aksesi Plasma Nutfah Kakao Berdasarkan Penanda Morfologi dan Molekuler Syafaruddin, Syafaruddin; Nasution, M A
Jurnal Tanaman Industri dan Penyegar Vol 3, No 2 (2012): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v3n2.2012.p177-184

Abstract

Kakao merupakan tanaman potensial, informasi lengkap termasuk molekuler sangat diperlukan untuk pengembangan ragam selanjutnya. Tujuan penelitian adalah mengevaluasi keragaman genetik, hubungan kekerabatan dan identifikasi kultivar tanaman kakao baik secara morfologi maupun molekuler.  Penelitian dilaksanakan di lapangan, yaitu di Kabupaten Pinrang dan Luwu Provinsi Sulawesi Selatan, serta di Laboratorium Biologi Molekuler, BB-Biogen, Bogor. Penelitian dimulai Maret sampai Desember 2011. Bahan tanaman yang digunakan 17 aksesi kakao. Selain pengamatan karakteristik morfologi, dilakukan juga analisa molekuler dengan beberapa tahapan: Isolasi DNA mengikuti metode CTAB Doyle dan Doyle, pemurnian DNA mengikuti metode Sambrook dan Russel, penetapan kualitas DNA, dan Reaksi amplifikasi dan elektroforesis mengikuti metode Williams. Sedangkan untuk uji similaritas digunakan software N-Tsys. Hasil penelitian menunjukkan bahwa dari 46 pola pita yang dihasilkan oleh 8 primer diperoleh 36 pita polimorfisme (78%) dan 10 pita monomorfik. Analisis kluster terhadap 14 karakter utama morfologi tanaman kakao menghasilkan dendrogram dengan koefisien kemiripan berkisar antara 65-98% atau terdapat keragaman genetik sebesar 2-35%.  Sedangkan analisis kluster terhadap 46 pola pita DNA menghasilkan dendrogram dengan koefisien kemiripan berkisar antara 64-91% atau terdapat keragaman genetik sebesar 9-36%. Hasil analisis secara morfologi maupun molekuler, keduanya menunjukkan variasi yang sempit.  Variation of 17 Cocoa Accessions Germplasm Based on Morphological and Molecular Marker ABSTRACT Cocoa is one of other important crops of Indonesia. Comprehensive information of cocoa is therefore needed, including molecular information for crop improvement.The purpose of this experiment was to find out information of genetic variation, genetic relationship and cultivar identification of cocoa by using morphology character and molecular analysis. The experiment was conducted at field in South Sulawesi and Molecular Biology Laboratory of BB-Biogen, Bogor since March till December 2011. Genetic material used were 17 accessions of cocoa, and other material were chemical substances. Besides morphological characteristics, molecular markers were also analyzed by using several steps: DNA isolation uses CTAB methods by Doyle and Doyle, DNA purification uses Sambrook and Russel methods, and amplification and electrophoresis reaction uses Williams methods. Whereas genetically similarity were analyzed by using N-Tsys. Result showsed that of 46 band patterns of DNA is resulted from 8 primers yielded of 36 band patterns of polymorphism (78%) and 10 band patterns of monomorphism.  Based on the cluster analysis of 14 main morphologycal characters, it was obtained a dendrogram with similarity coefficient about 65-98% or genetic variation about 2-35%. While cluster analysis to 46 band patterns of DNA was obtained a dendrogram with similarity coefficient about 64-91% or genetic variation about 9-36%. Based on morphologycal and genetic characters, both the results show narrow variation.
Rootstock Growth and Green Budding Success of Rubber Plant in Different Sizes of Polybag and Growing Media Rusli Rusli; Nana Heryana; Saefudin Saefudin
Jurnal Tanaman Industri dan Penyegar Vol 1, No 3 (2014): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v1n3.2014.p141-148

Abstract

Volume and type of growing media is important to support the growth of rubber seedling (Hevea brasiliensis) for rootstocks. The objective of this study was to determine the growth of rootstock and green budding success of rubber plants in different size of polybag and growing media. The research was carried out since January to December 2013 at the Pakuwon experimental garden, Parungkuda District, Sukabumi Regency, West Java. The study used a randomized block design (RBD) in factorial with two factors and three replications. The first factor is the sizes of polybag: (1) 30 ´ 20 cm, (2) 35 ´ 20 cm, and (3) 40 ´ 20 cm. Meanwhile, the second factor is the growing medium that consists of the mixture of soil and cow manure with 4 proportions: (1) 1 : 0, (2) 3 : 1, (3) 2 : 1, and (4) 1 : 1. Observations were made on the growth of pre-budding rootstock including seedling height, seedling diameter, number of leaves, and the success percentage of green budding. The results showed that the size of polybag has positive effect on the growth of the rubber seedling as rootstock at 6 months old after planting. Moreover, the use of large sizes of polybag (30 x 20–40 x 20 cm) was good for the growth of rootstocks that would be used for green budding. In addition, growing media that consisted of soil and cow manure at a comparison of 1 : 1 gave the highest effect on the growth of rootstock as well as the highest percentage of green budding success. However, there is no interaction between the size of polybag and growing media to the growth of rootstock and green budding success.

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