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INDONESIA
Indonesian Journal of Cancer Chemoprevention
Published by Indonesian Society for Cancer Chemoprevention
ISSN : 23558989     EISSN : 20880197     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Indonesian Journal of Cancer Chemoprevention (IJCC) is an open access, peer-reviewed, triannual journal devoted to publishing articles on Cancer Chemoprevention including Experimental and Clinical Pharmacology, especially concerning Anti-Oxidants, Anti-Aging, Anti-Inflammation, Anti-Angiogenesis, and Anti-Carcinogenesis; Cancer Detection; Stem Cell Biology; Immunology; in vitro and in silico Exploration of Chemopreventive Mechanism; and Natural Products.
Arjuna Subject : -
Articles 334 Documents
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Ethanol Extract of Artocarpus odoratissimus (Tarap) Bark Inhibit Migration of MDA-MB-231 Triple Negative Breast Cancer Cells Eva Rachmi; Nurul Hasanah; Hadi Irawiraman; Annisa Puteri Nuratifah; Fritz Nahusuly
Indonesian Journal of Cancer Chemoprevention Vol 14, No 2 (2023)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev14iss2pp83-93

Abstract

Breast cancer is the most diagnosed cancer in the world, with triple negative breast cancer (TNBC) being one of its subtypes that exhibits an aggressive clinical course, a tendency for early metastasis, and a poor prognosis. Tarap (Artocarpus odoratissimus Blanco) is an endemic plant of East Kalimantan, which possesses anticancer potential. This study aimed to explore the ability of ethanol extract of Tarap bark (EETB) in reducing cell viability, inducing apoptosis and inhibiting migration of MDA-MB-231 cells. Cell viability was observed by 3-(4.5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. EETB -induced apoptosis was analyzed by double staining allophycocyanin (APC) Annexin V with PI kit through flow cytometry. Cell migration was determined through scratch assay. EETB was found to decrease cell viability with an IC50 value of 1607.302 μg/ml (R=0.369). EETB’s ability to induce total apoptosis did not show significant differences across various concentrations. EETB was able to inhibit cell migration, especially at concentrations of 800 mg/ml, both at 24-h and 48-h observations. This finding demonstrates that EETB has a weak effect on reducing the viability and inducing the apoptosis of MDA-MB-231 cells. EETB induced morphological cells type change in to globular type and significantly inhibits two-dimensional migration of MDA-MB-231 cells.Keywords: Tarap, Artocarpus odoratissimus, triple negative breast cancer.
The Study of Molecular Docking and Molecular Dynamics Simulation Chemical Compound of Pycnarrhena cauliflora Diels. as Proapoptosis in Cervical Cancer Anggun Qurrota Aini; Supandi Supandi; Rosa Adelina; Dila Aulia Maharani
Indonesian Journal of Cancer Chemoprevention Vol 15, No 1 (2024)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev15iss1pp63-75

Abstract

Cervical cancer is one of the most common cancers among women worldwide and in Indonesia. B-cell lymphoma 2 (Bcl-2) can play a role in causing cancer by inhibiting apoptosis. The purpose of this study was to analyze the chemical compound of the sengkubak plant (Pycnarrhena cauliflora Diels.), which can act as antiapoptotic inhibitor by binding to the B-cell lymphoma 2 (Bcl-2) receptor. The research was conducted in silico with molecular docking methods and molecular dynamics simulations. Molecular docking used the AutoDock 4.2.6 software and visualization used Biovia Discovery Studio. Molecular dynamics simulation used Gromacs 5.1.2 software and result visualization used Grace. Longipinocarvone was the best test ligand with the smallest ΔGbind value of -6.99 kcal/mol compared to the positive control of Doxorubicin and other compounds which indicating Longipinocarvone’s affinity for binding to the Bcl-2 receptor was better than Doxorubicin. The types of interactions involved in the molecular docking of the chemical compounds of the sengkubak plant and Doxorubicin including hydrogen bonds and hydrophobic interactions. The stability of the bond between the ligand protein complex resulting from molecular docking was analyzed based on the parameters RMSD, RMSF, Radius of Gyration (Rg) values through molecular dynamics simulations. The results of the analysis showed that Longipinocarvone and Doxorubicin had a stable bond with Bcl-2 as indicated by the RMSD and RMSF values meeting the requirements, namely <3 Å (0.3 nm). The Rg graph showed both complexes are stable during simulation and have resemblant ligand-protein movements.Keywords: Cervical cancer, B-cell lymphoma 2 (Bcl-2), Pycnarrhena cauliflora Diels., molecular docking, molecular dynamics.
Apoptosis Induction of SKOV-3 Ovarian Cancer Cells from Pacing Rhizome (Costus speciosus) Through the Modulation of BAX and P53 Genes Expression Alifia Brilliani Hidayah; Nuqya Ashfannada; Aisyah Nur Khasanah; Siti Nur Annisa; Ghea Rachella Tiffany; Retno Murwanti
Indonesian Journal of Cancer Chemoprevention Vol 14, No 2 (2023)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev14iss2pp139-150

Abstract

Costus speciosus or Pacing has been investigated to have cytotoxic effect on several human cancer cells. Current was conducted to determine the cytotoxic activity of ethanol extract of the Pacing rhizome (EP) on SKOV-3 ovarian cancer cells, to investigate EP’s effect on BAX and p53 expression, and predict the inhibition activity on intrinsic pathway. EP were extracted using maceration method with 70% ethanol. The cytotoxic effect was performed using MTT Assay with various concentrations (375 μg/mL, 250 μg/mL, 62.5 μg/mL, 31.25 μg/mL, and 7.8125 μg/mL). The ability of EP to induce apoptosis in SKOV-3 cells was measured using flow cytometry. The BAX and p53 gene expressions on SKOV-3 cells were detected with RT-qPCR after treatment of EP with concentrations of ¼ IC50, ½ IC50, and IC50. The ability of diosgenin to interact with BAX and p53 can be seen from the interaction with the MCL-1 protein and was predicted with molecular docking. The results showed that IC50 of cytotoxic activity was 69.143 μg/mL. EP could induce apoptosis on SKOV-3 cells and can induce BAX and p53 protein expressions. The docking results show that diosgenin has the potential to act as an antagonist for the MCL-1 protein, it can increase apoptosis.Keywords: apoptotic, Costus speciosus extract, SKOV-3, p53, BAX.
Impact of Donor Age on Human Platelet Lysate Quality and its Consequential Effects on HeLa Cell Growth in the Presence of Anti-Cancer Compounds Diani Mentari; Gratiana Ekaningsih Wijayanti; Tuti Sri Suhesti; Katon Muhammad; Ni Ken Ritchie; Diah Nurpratami
Indonesian Journal of Cancer Chemoprevention Vol 14, No 3 (2023)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev14iss3pp189-198

Abstract

An integral aspect of anticancer experimentation involves delineating the optimal dosage of the test compound to ascertain its efficacy in targeting malignant cells. Numerous variables may influence a compound's cytotoxicity, among which is the choice of cell culture medium. Within in vitro settings, supplementary mediums are employed to foster cellular proliferation. Platelet lysate (PL) serves as a growth supplement, presenting an alternative to fetal bovine serum (FBS), primarily due to its incorporation of growth factors such as platelet-derived growth factor (PDGF), a component absents in FBS. The integrity of PL may be subject to various factors, including the age of the donor. This study sought to evaluate the impact of donor age on PL quality. Furthermore, it aimed to discern whether PL derived from platelet concentrate (PC) blood components of different age cohorts influences the IC50 value in anticancer compound assessment. Expired PCs were utilized, subsequently classified into age categories: ≤30 years, >30 years, and a combination of ages. PL analysis encompassed parameters such as pH, blood profile, protein, glucose, and cholesterol levels. The investigation scrutinized the influence of PL quality, as a cellular growth supplement, on the anticancer compound cisplatin's activity against HeLa cells. Findings indicate that donor age influenced the IC50 value of cisplatin on HeLa cells. Notably, elevated cholesterol levels and decreased pH in PL from donor ages >30 years were associated with reduced cisplatin toxicity.Keywords: Cisplatin, Donor Age, HeLa, IC50, Platelet Lysate.
Etlingera elatior Compounds as Anticancer Agents of Breast Cancer Through Inhibition of Progesterone Receptor: An In Silico Study Putri Mahirah Afladhanti; Haidar Ali Hamzah; Muhammad Despriansyah Romadhan; Safa Nabila Putri; Ellen Callista Angelica; Theodorus Theodorus
Indonesian Journal of Cancer Chemoprevention Vol 14, No 2 (2023)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev14iss2pp94-104

Abstract

Breast cancer is the leading cause of cancer-related death in women globally. Progesterone receptor (PR) is known as the prime example of receptors amenable to targeted breast cancer drug therapy. Etlingera elatior is an herbal plant that has been renowned to have anticancer effect. This study aimed to identify the potential compounds derived from Etlingera elatior as anticancer agents of PR in breast cancer using molecular docking method. This study used fifteen compounds from Etlingera elatior along with lonaprisan as the comparative drug. The PR was downloaded from RCSB, whereas compounds and lonaprisan were from Pubchem. The drug-likeness test based on Lipinski’s rule of five was conducted using SwissADME. Toxicity analysis using admetSAR 2.0 was used to predict toxicological profile of the compounds. Compounds and lonaprisan were docked on PR using AutoDock tools 1.5.6 and AutoDock Vina 1.1.2. Molecular interactions were visualized by Discovery Studio v16. A total of nine compounds met the criteria as drugs based on drug-likeness and toxicity tests. All nine compounds except caffeic acid and vanillic acid had higher binding affinities on PR compared with lonaprisan. Ergosterol peroxide exhibited the highest binding affinity on PR with values of -9.8 kcal/mol. Moreover, ergosterol peroxide-PR interaction had thirteen hydrophobic bonds and a hydrogen bond with amino acid residues were found in the active site of PR. Most of the compounds found in Etlingera elatior have the potential to be anticancer agents of PR in breast cancer with ergosterol peroxide being the most potential compound. Further in vitro and in vivo research are needed.Keywords: breast cancer, ergosterol peroxide, Etlingera elatior, progesterone receptor, in silico.
Bioinformatic Test and Pharmacokinetic Profile Prediction of Gnetin-C Compound in Melinjo (Gnetum gnemon L.) Seeds Toward Colorectal Cancer Ivana Kinanti Kenyori; Rifki Febriansah
Indonesian Journal of Cancer Chemoprevention Vol 15, No 1 (2024)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev15iss1pp18-25

Abstract

Cancer is one of the unresolved health problems in the world, including Indonesia. One of the most common types of cancer is colorectal cancer. Melinjo (Gnetum gnemon L.) is one of Indonesian local commodities which has many benefits including its potential to be developed as an anticancer agent. Through bioinformatics and molecular docking technology, the aim of this study was to investigate the functions of gnetin-C from melinjo against colorectal cancer. PkCSM database were used to search the ADMET (absorption, distribution, metabolism, elimination and toxicity) properties and stitch-string database were used to identify common genes related to colon cancer. During identification of colon cancer related genes, STAT3 protein showed the highest degree score. Furthermore, molecular docking was carried out to find out the interaction between the STAT3 protein and gnetin c compound found in melinjo seeds. From the docking stage, the pose with the best affinity energy was obtained with a docking score of 1,966 kcal/mol. this shows that the compound gnetin c has the potential to be used as a new anticancer agent from natural ingredients.Keywords: Melinjo, Gnetum gnemon, colon cancer, bioinformatics, molecular docking.
Citrus sinensis Peel Extract Synergistically Enhances the Cytotoxic Effect of Chemotherapeutic Agents on HepG2 Cells Shofa Khamdanatuz Zufairo; Desty Restia Rahmawati; Edy Meiyanto; Ratna Asmah Susidarti
Indonesian Journal of Cancer Chemoprevention Vol 14, No 3 (2023)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev14iss3pp151-159

Abstract

Doxorubicin (DOX) and cisplatin (Cis), non-specific chemotherapeutic agents used for hepatocellular carcinoma (HCC), are frequently combined with synthetic or natural agents to enhance their cytotoxic effects. Citrus sinensis peel extract (CPE) serves as a natural source of flavonoids, including sinensetin (SIN), which has the potential to increase the efficacy of DOX and Cis. This study aimed to observe the effect of CPE and SIN one of CPE compounds, in enhancing liver cancer cell susceptibility to doxorubicin and cisplatin. The assays conducted in this study included a phytochemical analysis of CPE using TLC, cell viability assays against HepG2 cells using MTT assay in both single and combination forms, and cell viability assays on Vero cells. The result confirmed the presence of SIN as one of the compounds in CPE. Both CPE and SIN, when used individually, exhibited moderate cytotoxic effects on HepG2 cells with IC50 of 101.09 μg/mL and 83.13 μM, respectively, while showing no cytotoxic effect on Vero cells. Cis demonstrated significant cytotoxicity against HepG2 cells with an IC50 of 7.86 μM. DOX exerted a strong cytotoxic effect on both HepG2 and Vero cells, with the IC50 of 2.52 μM and 13.98 μM. It was observed that CPE was able to synergistically enhance the cytotoxic effects of DOX, and SIN synergistically increased the cytotoxicity of Cis, particularly against HepG2 cells, with CI<1.0.Keywords: CPE, SIN, Cisplatin, Doxorubicin, HCC.
Analysis of SARS-CoV-2 spike-Induced Syncytia with Lifeact-GFP as Biosensor Using High-Content Screening Instrument for Automated Syncytia Counting Fauziah, Dita; Septisetyani, Endah Puji; Yerizel, Eti; Kastian, Ria Fajarwati
Indonesian Journal of Cancer Chemoprevention Vol 15, No 2 (2024)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev15iss2pp127-136

Abstract

SARS-CoV-2 is believed to cause cytopathic effects in forming multinucleated cells, known as syncytia. Syncytia due to SARS-CoV-2 infection found in lung tissue samples of COVID-19 patients represents a case of COVID-19 with a poor prognosis. Therefore, it is very important to study the mechanism of syncytia formation and to test candidate materials that can inhibit the occurrence of syncytia and potentially be applied in the treatment or prevention of COVID-19. Since syncytia counting and analysis are time-consuming, we utilized a high-content screening (HCS) instrument in this study to automate syncytia analysis. We used 293T cells transfected with plasmids to express the SARS-CoV-2 spike, human angiotensin-converting enzyme-2 (hACE-2), and a plasmid encoding lifeact-GFP as an F-actin biosensor to facilitate syncytia analysis using the HCS instrument. In this study, syncytia analysis was carried out using HCS software. The HCS application categorizes cells as multi-nuclei by counting the number of cell nuclei stained with DAPI in cells that emitted green fluorescence due to lifeact-GFP expression. Syncytia analysis is time-consuming because of the calculation of the number of syncytia formed in a confluent cell monolayer culture. Hopefully, utilizing the HCS platform can accelerate the test of syncytia inhibition after various treatments using test compounds.Keywords: 293T cells, high-content analysis, SARS-CoV-2, spike, syncytia.
Prediction of Secondary Metabolite Compounds in Lotus (Nelumbo nucifera Gaertn.) Targeting Androgen-α Receptors for Breast Cancer Treatment Using Molecular Docking Siagian, Virginia Heaven Mariboto; Destia, Melsa; Andini, Nasywa Putri; Yustiandini, Benedicta Andrea; Adhinagoro, Bagus; Salsabila, Shela; Muchtaridi, Muchtaridi
Indonesian Journal of Cancer Chemoprevention Vol 15, No 2 (2024)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev15iss2pp137-149

Abstract

Breast cancer (BC) is a malignant tumor that grows in the breast tissue and can spread to other organs. BC is often found at an advanced stage and therefore has a poor prognosis. With 68,858 cases, BC is the most common type of cancer in Indonesia. The development of breast carcinoma is strongly influenced by steroid hormones and their receptors, such as estrogen, progesterone, and androgen. Androgen receptors (AR) are found more (70-90%) compared to estrogen receptors (60-80%) and progesterone receptors (50-70%). Therefore, research on natural compounds that inhibit cancer cell proliferation influenced by AR needs to be increased. The lotus plant (Nelumbo nucifera Gaertn.) has been used as a traditional herbal medicine and food in Asia. Bioactive compounds in lotus have therapeutic potential against BC. In the pharmacophore screening results, five hit compounds were found: isorhamnetin, luteolin, catechin, kaempferol, and apigenin. The compound with the best pharmacophore fit score value is isorhamnetin with a value of 41.31, while the compound with the best binding affinity to AR is kaempferol with a binding affinity of -9.44 and an inhibitor constant of 121.12 nM.Keywords: breast-cancer, androgen-α receptor, lotus, molecular docking.
Evaluation of the Potential In Vitro effects of Plantago major L. on Wound Healing in Human Umbilical Vein Endothelial Cells (HUVEC) Amalia, Latifa; Murwanti, Retno; Hertiani, Triana; Purnomo, Kurnia Rahayu
Indonesian Journal of Cancer Chemoprevention Vol 15, No 2 (2024)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev15iss2pp87-95

Abstract

 The treatment of skin wounds remains a major concern in the field of medicine, particularly in the case of chronic wounds resulting from various disorders such as diabetes. The utilization of herbs or herbal preparations for the purpose of healing skin wounds presents a therapeutic challenge within the realm of traditional medicine. Plantago major L. is known to have bioactive compounds that have wound healing activity such as aucubin. This study aimed to determine the in vitro wound healing potential of Plantago major L. extract (PLE). The study involved several assays, including phytochemical examination of PLE using TLC, cell viability testing using MTT assay, and wound healing testing using scratch assay on human umbilical vein endothelial cells (HUVEC). The results confirmed the presence of aucubin as one of the compounds in PLE. It was observed that PLE with 125 μg/mL exhibited the highest wound closure percentage at 90.66%. This study shows that PLE possesses wound healing capabilities.Keywords: Plantago major L., PLE, cytotoxic assay, wound scratch assay, HUVEC.

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