cover
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota surakarta,
Jawa tengah
INDONESIA
Nexus Kedokteran Translasional
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Arjuna Subject : -
Articles 123 Documents
The Effect of Mangosteen Rind Extract (Garcinia mangostana L) to the Damaging of Hepar Cell of Mice (Mus musculus) Induced by Paracetamol Winda A Panjaitan; S B Widjokongko; Sigit Setyawan
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.207 KB)

Abstract

Background: The antioxidant properties of mangosteen rind extract such as xanthones, vitamin B1, vitamin B2, and vitamin C are estimated can protect hepar from free radicals and reducing NAPQI which produced by paracetamol metabolism. The purpose of this research is to know the effect of mangosteen rind ethanol extract to the damaging of hepar cell of mice induced by paracetamol. Methods: This research was a laboratorial experiment with post test only control group design. Samples of 28 male mice, Swiss webster type, 2-3 months old age and 20 gram of each weight, were divided into 4 groups in random or 7 mice each group. Aquadest were administered to mice in Control Group (KK) and the First Treatment Group (KP1), while mangosteen rind extract were administered to the Second Treatment Group (KP2) and the Third Treatment Group (KP3) with the dose of 10 mg/20 g body weight of mice and 15 mg/20 g body weight of mice. Paracetamol were administered to KP1, KP2, KP3, with dose 5.07 mg/20 g body weight of mice. On day 4th, mice were sacrificed with neck dislocation. Hepar cell slides were made and stained by HE. The hepar cell damage of mice were observed by counting number of necrosis cell. The data was analized by One-Way ANOVA test (? = 0.05) and continued by Post Hoc Multiple Comparisons using Least Significant Differences (LSD) test (? = 0.05). Results: The data showed that average number of necrotic nucleus of KK was 9.71; KP1 was 72.86; KP2 was 14.00; and KP3 was 22.29. The results of One-Way ANOVA test showed a significant difference in all group (p < 0.05). The statistical data result showed a significant difference of necrotic nucleus between K-KP1, K-KP2, K-KP3, KP1-KP2, KP1-KP3, and KP2-KP3 (p < 0.05). Conclusion: The feeding of mangosteen rind was able to decrease hepar cell damage of mice which is induced by paracetamol. Keywords: mangosteen rind extract, paracetamol, the damaging of hepar cell
Antioxidant Effect of Bilimbi Fruit (Averrhoa Bilimbi Linn) Extract on SGPT Level in Mice Induced by Reheated Palm Oil Rosi Dwi Mulyono; R Prihandjojo Andri Putranto; Sarsono .
Nexus Kedokteran Translasional Vol 5, No 2 (2016): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Introduction: Reheated palm oil was known to contain free radicals that cause damage and death of liver cell, antioxidant is needed to prevent. One source of antioxidant is Bilimbi fruit. This research aims to determine the effect of Bilimbi fruit extract and variant in dosage of Bilimbi fruit extract on levels of Serum Glutamic Piruvic Transaminase in mice induced by reheated palm oil. Methods: This research was a laboratory experimental with a post-test only control grup design. The subject of research was Swiss Webster mice, male, age 2-3 mounths, weight + 20 grams. The subject ware chosen by incidental sampling for 30 individuals, devided randomly into 5 groups, each groups consist of 6 mice. The control group (K) was given aquadest (0,3 ml/20 g BW and 0,2 ml/20 g BW). Mice in treated group 1 (P1) was given aquadest (0,3 ml/20 g BW) and reheated palm oil (0,2 ml/20 g BW). Mice in treated group 2 (P2), treated group 3 (P3) and treated group 4 (P4) was given Bilimbi fruit extract with dosage 5,6 mg/20 g BW; 11,2 mg/20 g BW; and 22,4 mg/20 g BW and reheated palm oil (0,2 ml/20 g BB). Bilimbi fruit extract was given 1 hour before reheated palm oil for 14 days. At the day-15, mice ware sacrificed and the animal blood was collected from pleksus retroorbitalis. The result was analyzed statistically by one way ANOVA and followed by LSD test (? = 0,05). Results: Mean of SGPT level in each group was K = 37,5 + 8,256 U/L; P1 = 50,92 + 8,267 U/L; P2 = 37,08 + 5,043 U/L; P3 = 43,34 + 7,834 U/L; P4 = 51,12 + 13,526 U/L. There wes significant difference between K P1, K P4, P1 P2, and P2 P4, there was not significant difference between K P2, K P3, P1 P3, P1 P4, P2 P3, and P3 P4. Conclusions: The extract of Bilimbi fruit decrease SGPT level in mice induced by reheated palm oil. An increase in dose of Bilimbi fruit extract cannot increase the influence to decrease SGPT level in mice induced by reheated palm oil. Keywords: Bilimbi fruit extract, SGPT, reheated palm oil, SGPT.
Pengaruh Pemberian Topikal Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) Terhadap Jumlah Sel Fibroblas Pada Penyembuhan Luka Sayat Pada Kulit Tikus Putih (Rattus norvegicus) strain wistar Dentiko Wasis Aulia; Brian Wasita; Riza Novierta Pesik
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.207 KB)

Abstract

Background: Binahong (Anredera cordifolia (Ten.) Steenis) is a common plant in Indonesia and contain many secondary metabolites such as flavonoids, tannins, saponins, terpenoids, and alkaloids. These secondary metabolite substances were expected to increase the number of fibroblast cells in the wound healing process. This study aims to demonstrate that topical administration of binahong leaf extract jelly on Wistar rats (Rattus norvegicus) skin cut will increase the number of fibroblasts in these wound healing process. Methods: This is a laboratoric experimental study with randomize post test only control group design. The samples were 25 male Wistar rats aged 3 months and given a dorsal incision of 2 cm long and 0.5 cm deep. The rats were divided randomly into 5 groups (positive control, negative control, and 3 treatment group) consisting of 5 rats each. Positive control group were topically treated with patent medicine Bioplacenton. Negative control group was not given any treatment. Treatment groups were topically treated with a binahong leaf extract jelly 2.5%, 5%, and 10% respectively. Bioplacenton and binahong leaf extract jelly is given 2 times a day on its wound for 5 days. On the fifth day, all rats wound were scored using Bates-Jensen Wound Assassment Tool and then sacrificed to make histophatology slides on the part of the injured skin. The slides were stained with Van Giesson staining and the fibroblast in the granulation tissue was calculated. The data obtained was analyzed with Oneway ANOVA (p <0.05). Result: The macroscopic wound scoring and average number of fibroblast cells in each group showed no significant difference (p>0.05). However, in the 2nd treatment groups (binahong leaf extract jelly 5%) showed the highest number of fibroblasts (153.72 (SD 23.39)) than the other groups. Conclusion: Topical administration of binahong leaf extract (Anredera cordifolia (Ten.) Steenis) did not affect the macroscopic wound scoring but it increase the number of fibroblast cells in healing skin cuts on Wistar rats (Rattus norvegicus) even thought it did not reach the significant level. Keywords: wound healing, fibroblast, binahong (Anredera cordifolia (Ten.) Steenis)
The Effect of Beans Extract (Phaseolus vulgaris L.) to LDL Cholesterol Level in White Rats Hypercholesterolemia Model Noni Kartika Sari; Ida Nurwati; Danus Hermawan
Nexus Kedokteran Translasional Vol 5, No 1 (2016): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Introduction: Low Density Lipoprotein (LDL) cholesterol level related with Coronary Heart Disease (CHD) which is the most common death cause in the world. Beans contains flavonoid, phytosterol, saponin, niasin, vitamin C, and fiber which have hypolipidemic effect. This study aimed to determine the effect of beans extract to the LDL cholesterol level in white rats hypercholesterolemia model. Methods: This study was a laboratory experimental research using the posttest only controlled group design, that have done in Histology Laboratorium of Sebelas Maret University Surakarta. Samples of research were 30 male white rats, Wistar strain. Control Group (KK) was given pellets and aquades for 28 days. Group 1 until 4 (KP1-4) were given duck egg yolk 5 gram/200 gram BW white rat/day and PTU 0,01% for 28 days. On days 15-28, Group 2 (KP2) was given simvastatin 0,72 mg/200 gram BW white rat/day, Group 3 was (KP3) given beans extract 100 mg/200 gram BW white rat/day, and Group 4 (KP4) was given beans extract 150 mg/200 gram BW white rat/day. The datas of LDL cholesterol level were analyzed with One-way ANOVA test and Post Hoc test. Result: Result of this study shows that LDL cholesterol levels of KK = 33.83 14.442 mg/dl, KP1 = 54.00 27.943 mg/dl, KP2 = 18.83 7.672 mg/dl, KP3 = 77.33 20.954 mg/dl and KP4 = 80.17 31.923 mg/dl. The statistical analysis with One- way ANOVA shows a significant difference in LDL cholesterol level among the five groups of treatment with p = 0.000 (p < 0.05). Post Hoc Test shows a significant difference in LDL cholesterol level between KP1 KP2 with p = 0.011 (p < 0.05) and non significant difference in LDL cholesterol level between KK KP1 with p = 0.129, KP1 KP3 with p = 0.081, and KP1 KP4 with p = 0.052 (p > 0.05). Conclusions: The beans extract (Phaseolus vulgaris L.) does not give any effect to decrease of the LDL cholesterol level in white rats (Rattus norvegicus) hypercholesterolemia model. Keywords: Beans extract, LDL, Rattus norvegicus, hypercholesterolemia.
Pengaruh Ekstrak Daun Kemangi terhadap Penurunan Kadar Kreatinin dalam Darah Tikus Putih yang Diinduksi Parasetamol Arianto Adi Wibowo; Endang Ediningsih; Samigun .
Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.205 KB)

Abstract

Background: Induced by parasetamol in large quantities will damaging in proximal tubule. Creatinine which should secreted by glomerolus will back in blood stream if occur kidney obstruction that which decreasing in-blood levels of creatinine. The purpose of this research is to find out whether the extract of kemangi has an effect to decrease in-blood levels of creatinine in white rats induced by parasetamol. Methods. The research was an experimental laboratoric study with the post test only controlled group design. This study was done in Setia Budi University. Subjects in this research were tirthty male white rats (Rattus norvegicus), 2-3 month of age and 150-220 gram of each weight. Subjects were divided into 5 groups.Group I as a negative control only received normal saline. Group II as a positive control and group III, IV, and V received parasetamol (150 mg/200 g body weight) every 3 day for 10 days. Group III received extract kemangi at 40mg / 200g body weight every 3 day for 10 days. Group IV received extract kemangi at 80mg / 200g body weight every 3 day for 10 days. Group V received extract kemangi at 120 mg 200 g body weight every 3 day for 10 days. Treatment were conducted on 11st after a 10-day adaptation. Blood sampling via plexus vena orbita of white rats for creatinine levels measured were conducted on 21st day. Then data was analyzed using One-Way ANOVA and LSD. Result. Result of One-Way ANOVA showed that there was a significant different between 5 groups: positive and negative control groups, and three dosages difference treatment (40mg, 80mg, 120mg / 200gr BB). Result of LSD showed that there was a significant different between positive control group and treatment groups; and between control group, but there was not the significant different between negative control group and treatment groups; and each treatment groups. Conclusion. All of the extract kemangi dosages has significantly effect to decrease in- blood levels of creatinine in white rats induced by parasetamol. Statisticaly, there is no significant different in the dose diffenrence at treatment 1, 2 and 3 (40mg, 80mg, 120mg / 200gr BB). Keywords: extract kemangi, decrease in-blood levels of creatinine, parasetamol.
Identification of Herbal Compounds as Thymidylate Synthase Inhibitors that more Potent than 5-Fluorouracil using Molecular Docking Yoga Mulia Pratama; Dono Indarto; Yuliana Heri Suselo
Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (678.827 KB)

Abstract

Background: Development of new selective anticancer drug is an important necessary. Because of that, some effective way to screen herbal compounds which have good potency for developing drug is needed, one of them is using molecular docking methods. In this study, researchers identify herbal compounds as new thymidylate synthase (TS) inhibitors which more potent than 5-Fluorouracil (5-FU) using molecular docking. Methods: Three-dimensional structure samples of herbal compounds from Indonesian herbal database (herbaldb.ui.ac.id) were docked with three-dimensional structure of TS protein using iGEMDOCK program. Then, the compounds which have lower docking score than fdUMP, as standard ligand, clustered using ChemMine. Results: The docking result showed that 27 herbal compounds have docking score lower than fdUMP in iGEMDOCK program. The 27 compounds have higher affinity to fdUMP and it can form a stable complex with TS protein. Protein-ligand complexes showed that all of 27 compounds have same binding site with fdUMP. But folic acid is excluded from the result because it is a TS ligand. fdUMP has similar structure with UMP, a TS ligand, but folic acid which has lowest docking score is not similar with UMP. Conclusions: The 26 compounds, except folic acid, are more potent than 5-FU as TS inhibitors by docking study. Further study to delineate the effectivity of molecular docking is needed. Keywords: 5-Fluorouracil, herbal compound, molecular docking, thymidylate synthase
Pomegranate Extract Does Not Inhibit Sodium Glucose co-Transporter 2 Protein in Vero Cells Mila Ulfia; Dono Indarto; Amelya Augusthina Ayu Sari; Yuliana Heri Suselo
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Backgrounds: Mutation of SLC5A2 gene which encodes sodium glucose co-transporter2 (SGLT2) protein enhances glucose reabsorption on the kidney tubule in some patients with type 2 diabetes (DMT2). Dapagliflozine an oral antidiabetic drug, inhibits SGLT2 activity. Ellagic acid is able to inhibit SGLT2 protein in silico and highly found in pomegranate fruits. The aim of this study was to investigate the effect of pomegranate extracts on glucose levels in a model cell of African green monkey (Vero cell line). Methods: This study was an experimental laboratory with posttest only control group design. 1 x 106 Vero cells perwell were used in five experimental groups: negative control 1 (KKn1), KKn with 20% glucose (KKn2), positive control with dapagliflozine (KKp), ethanol and diethyl ether extract of pomegranate peel (KEDA), methanol extract of pomegranate seeds (BMA). Vero cells were then treated with 125 ppm pomegranate extracts (KEDA and BMA) and incubated for 24 hours. Cell morphology was observed under an inverted microscope with 100 x magnification. Glucose levels in Vero cells were measured using spectrophotometer. Collected data was analyzed descriptively. Result: Morphology of Vero cells was oval, soliter and centered nucleus and did not change during incubation with pomegranate extracts. Glucose levels in Vero cells treated with BMA (28.5 mg/dL) and KEDA (29 mg/dL) were higher than glucose levels in control groups KKp, KKn1, and KKn2 (2.5, 6.5 and 8 mg/dL respectively). Conclusion; Pomegranate extracts do not inhibit SGLT2 protein and increase glucose levels in Vero cells. Purification of pomegranate extracts is required for further investigation of the capability of ellagic acid inhibiting SGLT2 protein. Keywords: Ellagic acid, glucose level, pomegranate, SGLT2, type 2 diabetes.
Metformin increases protein expression of Bax and p21 in WiDr cancer cell line Melani Ratih Mahanani; Dyah Ratna Budiani; Ari Natalia Probandari
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.207 KB)

Abstract

Background: Colorectal cancer is malignancy in the gastrointestinal tract which leads to high morbidity and mortality rates globally. Colorectal cancer is the fourth highest cancer in Indonesia. 5-Fluorouracil (5-FU) is the first choice of chemotherapy for treating colorectal cancer but this therapy isless effective due to severeadverse effects. Metformin, a biguanidediabetes drug, has a potential anticancer, which is able to inhibit growth of some cancer cell lines like breast cancer, prostate cancer, hepatocellular cancer, and nasopharynx cancer. The aim of this study was to investigate protein expression ofBax (pro apoptotic protein) and p21 (cell cycle inhibitor) on colorectal cancer cell line treated with metformin. Methods: This study was a laboratory experimental study with the posttest only control group design. WiDrcancer cell line was cultured in RPMI1640 medium supplemented with 10% fetal bovine serum, 100 U penicillin and 100 g/ml streptomycin and incubatedin 5% CO2for 48 h at 37 C.Following day, cellswere treated with medium only, 7.68 mM5-FU and various doses of metformin(20, 10 and 5 mM) respectively. Protein expression of Bax and p21 was assessed by using immunocytochemistry staining against monoclonal antibodies antihuman Bax and p21.Intensity staining of each cell group was cytological scored and statistically analyzed by using Kruskal Wallis test. Results:Higher intensity score of Bax immunostaining was observed in WiDr cell line treated with three doses of metformin, compared to that of incontrol WiDr cell line and reached significantly difference with p= 0,00. Interestingly, these intensity scores of immunostaining were higher than the intensity score of immunostaining in WiDr cell line with 5-FU treatment. Moreover, administration of metformin increased intensity staining score against p21 monoclonal antibody with negatively dose dependent manner. WiDr cell line administered with 5 mM metformin had the highest intensity score of immunostaining but the intensity score was lower than the intensity score of immunostaining in WiDr cell line with 5-FU treatment. Conclusion: Metformin up regulates protein expression of Bax and p21 in WiDr cancer cell line and may become a promising anticancer. Further investigation is required to confirm the up regulation of both proteins. Keywords: Bax,colorectal cancer, metformin, p21
Protective Effect of Orthosiphon stamineus Benth Leaves Extract against Reheated palm oil-induced Liver Damage in Mice Fitroh Annisah; Bambang Widjokongko; Siti Utari
Nexus Kedokteran Translasional Vol 2, No 2 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.207 KB)

Abstract

Background: Orthosiphon stamineus Benth contains polyphenols, terpenoids, oleanolat acids, and sterols which were thought to protect the liver from free radicals. In present study, Orthosiphon stamineus Benth leaves extract was evaluated for its protective effect on reheated palm oil -induced liver damage in mice. Methods: This was laboratory experimental research with the post test only controlled group design. Twenty five male Swiss webster mice were equally divided into five groups: group I (Kn) received aquadest, group II (Kp) received reheated palm oil, and grup IIIV (P1-P3) received reheated palm oil and multilevel doses of Orthosiphon stamineus Benth leaves extract (3.5 mg, 7 mg, and 14 mg) for 14 days. Mice were sacrificed on the 15th day and histological preparation was made to evaluate histological damage on liver. Liver histological features were assessed by counting the number of hepatocyte on centrolobuler zone undergoing pyknosis, karyorhexis, and karyolysis. Data were analyzed using the One Way ANOVA test (? = 0.05) and the Post Hoc Multiple Comparisons (LSD) test (? = 0.05). Results: The mean liver histological damage score was significantly higher on group of Kp= 74.40 2.074, while group of Kn= 38.20 3,564 as the less. P2 group had fewer numbers of liver histological damage (57.00 4.472) compared to the P1 group (61.00 2.550) and the P3 group (58.60 4.775). The results of One Way ANOVA test showed significant difference between the five groups (p = 0.000) and the results of LSD test showed significant differences between each other group (p < 0.005), except the group of P1-P2, P1-P3, and P2-P3 (p > 0.005). Conclusions: Orthosiphon stamineus Benth leaves extract showed protective effect against the hepatotoxicity induced by reheated palm oil in mice. Orthosiphon stamineus Benth leaves extract with the dose of 7 mg/25 g body weight of mice showed a higher hepatoprotective effect than the dose of 3.5 mg/25 g body weight of mice and 14 mg/25 g body weight of mice. Keywords: Orthosiphon stamineus Benth leaves extract, reheated palm oil, histologic liver damage.
The Effect of Ethanol Extract of Cats Nettle Leaf (Acalypha indica Linn) to The Mortality of Aedes aegypti Larvae Yunika Varestri Anugrah Rizki; FX. Bambang Sukilarso Sakiman; Yusuf Ari Mashuri
Nexus Kedokteran Translasional Vol 5, No 1 (2016): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Introduction: The incidency of Dengue Hemorrhagic Fever (DHF) in Indonesia is high. The best way of prevention is cutting the infection chains, one of them is using larvasida. Methanol extract of cats nettle leaf is known as the killer of Aedes aegypti larvae in India. Here, the study is to examine if ethanol extract of cats nettle leaf in Indonesia has same larvasida affect toward Aedes aegypti. It is because the cats nettle leaf in Indonesia may have different variations. Methods: The study was a laboratoric experimental which was performed in Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga, Central Java. The subject was cats nettle leaf which was obtained from Sleman, Yogyakarta. Also, the indicator was 750 third instar Aedes aegypti larvas which were obtained from B2P2VRP Salatiga, Central Java. Ethanol extract of cats nettle leaf was obtained from Pharmacology Laboratory of Gadjah Mada University. The study was a post test only control group design with 1 control group and 5 treatment groups. Each group was consisted of 25 larvae and was examined four times. Aquades of 100 ml was administered to the control group. While, the ethanol extract of cats nettle leaf with concentration of 0,03%, 0,06%, 0,09%, 1,2% and 1,5% were administered to the treatment group. After 24 hours, observation and calculation of total larvae death were established. The data was analyzed by using Probit Regression and Curve Estimation. Results: The percentage of dead larvae in 0,03%, 0,06%, 0,09%, 1,2% and 1,5% of cats nettle leafs ethanol extract were 58%, 63%, 71%, 91% dan 94% respectively. The statistic result of Probit Regression shows that LC50 and LC99 are on 0,029% and 0,61% concentration respectively. Also, the Curve Estimation result shows that there is effect of ethanol extract of cats nettle leaf to the mortality of Aedes aegypti larvae with tight correlation R = 0,888 and the linear regression equation is Y = 0,478 + 3,073 X. Conclusion: The ethanol extract of cats nettle leaf induced effect to the mortality of Aedes aegypti larvae with LC50 on 0,029% concentration and LC99 on 0,61% concentration. Keywords: Aedes aegypti larvae, etanol extract of cats nettle leaf, LC50 and LC99

Page 12 of 13 | Total Record : 123