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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

112 113 114 115 116

Identification of volatile compounds from myiasis wounds and its responsesfor Chrysomya bezziana April H Wardhana; Sukarsih .; R Urech
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 1 (2005): MARCH 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Development of attractant for screwworm fly was required in myiasis control on livestock. The purpose of this study is to identify of volatile compounds from myiasis wound infested with Chrysomya bezziana larvae including to assess their responses in both cage and room assays. Both Friesian-Holstein heifer (FH) (animal 1) and Bali cattle (animal 2) were used as myiasis model. The artificial wounds (8-10 cm) were conducted on the rump of both animals and infested with about 200 eggs of C.bezziana. Odours from the infested wound were collected on day 1 and 3 for animal1 and day 3 and 5 for animal 2, post C. bezziana larvae infestation. Two different collection devices were used: firstly, absorption onto Tenax kept in steel tubes, whichwas attached to a collected bowl. The volatile organic compounds were collected from the wound and the surrounding animal hide by flowins the air through the inlet and outlet. Secondly, a solid phase micro extraction (SPME) device was inserted into bowl with passive (no air flow) odour collection. Gass chromatography/mass spectrometry was used to identify volatile compounds from wound. The compounds of the wound on animal 1, collected on day 1, produced only minor quantities of compounds (nonanal, decanal, hexanal and heptanal). Minor components such as DMDS and DMTS were only detected on day 3. The compounds of the wound on animal 2 was more varied and had a peculiar strike-like smell on day 3 and 5. They included indole, phenol, acetone, various sulfides (DMS, DMDS, DMTS), alcohols (butanol, 3-methylbutanol), aldehydes and acids. These compounds were selected and formulated into attractant (B92) then tested in both cage and room assays using SL-2 as control. Respond of flies was analyzed by ANOVA 5% (cage assay) and T test 5% (room assay). The result showed that the fly response to B92 was very low compared to SL-2 in cage assays (P<0.05). The addition of B92 to SL-2 could not increase the catch of flies in the cage assays (SL-2+B92=10:1; 10:3), there was no difference between SL-2 and B92/SL-2 in room assay, the fly response still low (P>0.05). Key Words: Volatile Compound, Myiasis, Wound, Chrysomya bezziana

Antibody response and protection of inactivated-local isolate vaccine for infectious bronchitis in laying chicken Risa Indriani; Darminto .
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 2 (2001): JUNE 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Infectious bronchitis (IB) is an acute highly contagious viral respiratory disease of poultry caused by Coronavirus. IBV infection consists of many serotypes and can only be controlled by vaccination. An effective IB vaccine should be prepared from local isolates, due to the antigenic variation among serotypes. The aims of this research were to develop inactivated IB vaccine derived from IBV local isolate and to determine the efficacy of that vaccine in layer flocks. Five layer chicken groups were used in this experiments, group I was vaccinated with commercial IBV live vaccine thrice, group II was vaccinated with commercial IBV live vaccine once and repeated with inactivated local IBV isolate twice, group III was vaccinated with commercial IBV live vaccine once and repeated with commercial inactivated twice, group IV was vaccinated with IBV live vaccine once, and group V was not vaccinated. After the chickens reached at a stable egg production they were challenged with IBV local isolates. Antibody responses were examined by means of haemagglutination hibitition (HI) test and HI titres were expressed as log2 of the reciprocal of the highest dilution of serum causing inhibition of a log2 HA titre of 2. The mean titres of antibody responses of chicken in group I, II, III, IV, and V was 4.9 ± 0.87, 6.8 ± 0.97, 7.7 ± 0.46, 2.9 ± 0.94, and 2.0 ± 1.67 respectively. The levels of protection against challenges were determined by viral isolation, this in group I, II, III, IV, and V was 63, 73, 60, 50, and 0% respectively. Clinical symptom of egg quality was slightly reduced in group I, IV, and V and it were unchanged in group II and III. Group II gave better in number of egg production than the other groups. The results indicated that the IBV inactivated localisolate vaccine gave high titres of antibody and higher protection rates than that of commercial IBV inactivated vaccine. Inaddition, IBV local isolate vaccinated group prevented from declining egg production after challenged with IBV local isolate. Key words: Infectious bronchitis, layer, antibody titre, vaccine, challenge virus

Performance of native chicken given free choice feed I.P Kompiang; Supriyati .; M.H Togatorop; S.N Jarmani
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 2 (2001): JUNE 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An experiment was conducted to study the effect of free choice feeding on the performance of native chicken and to determine its protein and energy requirements for growth. Eight hundreds and seventy 2-weeks old native chicken were used, and divided into three treatment groups, with 5 replicates: (I) Complete feed (II) Choice feed A (two levels of protein, similar in energy content) and (III) Choice feed B (energy and protein sources). Feed and water were given ad libitum during the 10 weeks trial. Feed intake (protein and energy) and body weight were recorded biweekly. Body weight gain (BWG) of treatment I was 852 + 33 grams/head, similar to treatment II, 858 + 28 grams/head, and both significantly (P<0.05) better than treatment III (800 + 42 grams/head). Energy consumption and its energy efficiency ratio (EER) value were similar for all treatments, 9226 + 149; 9203 + 739, and 8706 + 383 kcal/head and 10.84 + 0.34, 10.75 + 1.03, and 10.89 + 0.27 kcal/gram for treatment I, II and III, respectively. There was a significant (P<0.05) difference between treatment on protein intake, 556 + 8, 506 + 15, and 454 + 25 grams/head for treatment I, II, and III, respectively. Protein energy ratio (PER) value of treatment III (0.57 ± 0.02) was similar to treatment II (0.57 ± 0.02) and both were significantly better (P<0.05) than treatment I (0.65 ± 0.02). It was concluded that the native chicken, given a correct choice of feed, has an ability to determine its energy and protein requirements. During the growth periods, 2-12 weeks old, the bird require feed with 16% protein and 2900 kcal metabolized energy/kg. Key word: Native chicken, feeding system, free choice

Polymorphism of Calpastatin gene and its effect on body weight of local sheeps C Sumantri; R Diyono; A Farajallah; I Inounu
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The objectives of this research were to identify polymorphism of calpastatin gene and to investigate any association of calpastatin genotype on body weight of local sheeps. A total number of DNA samples were collected from 288 heads of local sheeps from 8 populations. Two local sheep samples were medium tail sheeps (MTSs) of a Garut fighting type from Ciomas/Bogor (29) and a Garut meat type from Margawati (29). The remaining six local sheep population were one thin tail sheep (TTS) from Jonggol (36); and five fat tail (FTSs) from Indramayu (43), Madura (43), Sumbawa (26), Rote (36) and Donggala (46) respectively. Genomic DNAs of those blood of local sheeps were extracted by a standard phenol-chloroform protocol and amplified using a polymerase chain reaction (PCR) technique. PCR reaction was carried out in a thermocycler (Takara PCR of Thermal Cycler MP4) and PCR products were digested with Msp 1 enzyme restriction using a Restriction Fragment Length Polymorphism (RFLP) technique. The PCR-RFLP products were separated at 8% polyacrylamide gel electrophoresis (PAGE). A silver-staining method then was applied to detect fragments. Genetic variations between local sheep populations were calculated based on frequencies of genotypes and alelles. The association between genotype of calpastatin gene and body weight of local sheeps were calculated by General Linear Model method by SAS version 6.12. A length of 622 base pairs (bp) of the calpastatin gene of the Indonesian local sheeps was successfully amplified by the PCR technique. An MspI restriction enzyme cut the PCR product into two different length fragments, those were 336 bp and 286 bp designated as M allele of the CAST-Msp1; whilst that unsuccessfully cut PCR product resulted one fragment 622 bp designated as N allele of the CAST-Msp1. Locus of the CAST-Msp1 gene in most local sheeps studied was polymorphic, the exception was in the FTS from Rote of which monomorphic. The highest frequency of the M allele was in the fighting Garut sheep from Ciomas (0.29), whilst the lowest was in the FTS from Rote (0.00). However, frequencies of the M allele of FTSs from Sumbawa and Madura were similar (0.04). Further, frequencies of the M allele of local sheeps from Margawati, Jonggol, Indramayu, and Donggala were 0.24, 0.16, 0.13 and 0.12 respectively. The highest frequency of MN genotype was observed in the Garut fighting sheep from Ciomas (0.58), but the lowest was in the FTS from Rote (0.00). The heterosigosity was observed differently among populations. The highest heterosigosity was also identified in the Garut fighting sheep in Ciomas (0.43), whilst FTSs both in Sumbawa and Madura were for the lowest (0.08). Results of this study showed that there was a definit association betwen calpastatin genotype and body weight of male sheeps from which the MN genotype significantly related to a higher body weight compared to that of the NN genotype. Key Words : Local Sheeps, Calpastatin Gene, Polymorphism, Live Weight

Effects of irradiation on the survival of bacterial contaminants in food Lily Natalia; A. Priadi; Z. Irawati
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 1 (2009): MARCH 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The primary concern about microbial safety of irradiated food is the survival of pathogenic spore forming bacteria. Clostridium sporogenes was selected as the spore forming test organism for conducting inoculated pack studies for its similarities to the most toxigenic Cl. botulinum, in radiation resistance. Minimum radiation dose applied (45 kGy under cryogenic condition, in -790C) was determined to eliminate Cl. sporogenes spores and other bacterial contaminants in different kind of Indonesian chicken and beef dishes. In separate studies, irradiation doses of 3 – 7 kGy at cryogenic condition was used to improve the microbiological safety of a number chilled prepared meals. The dishes or ready to eat foods were packaged in air impermeable pouches. Irradiation process was carried out after inoculation on chicken and beef dishes with certain amounts of Cl. sporogenes spores. The evaluation of colony count differences between the irradiated and unirradiated foods revealed the effect of radiation on the survival of bacterial spores or other bacterial contaminants. It was demonstrated that a minimum dose of 45 kGy under cryogenic condition eliminated the spore of Cl. sporogenes, Bacillus spp and Staphylococcus spp. Irradiation at doses 5-7 kGy significantly reduced some potential pathogenic microorganisms in samples without affecting quality up to 3 months of storage at the refrigeration temperature. Key Words: Food Safety, Irradiation, Bacterial Contaminants

Concentrate supplementation for crossbred bulls to increase profitability of smallholder fattening operations in East Java, Indonesia Ratnawati D; Cowley F; Mayberry D; Poppi D
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 1 (2015): MARCH 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Growth rates of bulls in Indonesia raised in smallholder fattening systems are generally low due to inadequate nutrition. This study compared bull growth and farmer profit between two feeding management systems, namely traditional feeding (as control) and intervention feeding by supplementation with a high quality concentrate. Bos taurus-Ongole cross bred bulls (n=46) aged 1.5-2 years, owned by smallholder farmers, from two villages in East Java were used in this study. The bulls were divided into two treatment groups; control and intervention. Farmers in the intervention group were provided with a concentrate containing onggok, copra and palm kernel cake. Farmers were instructed to feed 4 kg DM of concentrate/bull/day in addition to their existing roughage diet for 161 days or until sold. No changes were made to the feeding or management of the bulls in the control group. Mostly control group farmers used local forages and agricultural by product as a feed, without any supplementation. The daily feed cost and the cost of 1 kg of liveweight gain was higher in the intervention group (IDR 8827 and IDR 11990, respectively) than that of the control group (IDR 2606 and IDR 5543, respectively). Moreover, the average daily gain of bulls in the intervention group (0.82 kg/bull/day) was higher than that of bulls in the control group (0.52 kg/bull/day), resulting in a greater profit for the fattening period. Daily income minus feed costs was higher for farmers in the intervention group (IDR 24182/day), compared to farmers in the control group (IDR 15774/day). It is concluded that although there were additional costs for feeding high-quality feeds, but higher growth rates of bulls resulted in greater profits for smallholder farmers.

Pathogenicity of local isolates of Erysipelothrix rhusiopathiae serotypes 1, 2, 6, ! 1, 12, and type N in mice and pigs Siti Chotiah
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 4 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Six local isolates of Erysipelothrix rhusiopathiae of serotypes 1, 2, 6, 11, 12 ; ?,.;d type N isolated from the tonsil of healthy pigs at Kapuk, Jakarta, slaughter house were examined for their pathgb-;ucity for mice and pigs . Forty-two groups of mice, each contains ten mice and twenty-one pigs divided into seven groups were used as experimental animals. Isolates of serotype 1, 2, 6, 11, 12, and type N were pathogen for rnil.e with LDSO of 10", 102.", 10°6 ,103.° , 10 32 and 105A colony forming unit (C .F .U .), respectively. All (100%) pigs inoculated with isolate of serotype 1 and 33.3% pig inoculated with isolate of serotype 2 were induced general urticarial lesions with depression, anorexia and followed by death. One of three (33.3%) pig inoculated with isolate of serotype 6 was induced generalized urticarial lesions without any other clinical signs. Isolates of serotype 6, 11, 12, and type N were capable of inducing localized urticarial lesions in two out ~f three (66.7%), all (100%), two out of three (66.7° n all (100%) pigs, respectively . All pigs in control uninoculated Key words : Erysipelothrix rhusiopathiae, pathogenicity, mice, pigs

Infection of Ornithobacterium rhinotracheale (ORT) in chickens in Indonesia Adin Priadi; Lily Natalia
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 1 (2006): MARCH 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Ornithobacterium rhinotracheale is a bacterium identified as a new species in 1994 and generally associated with respiratory distress in chickens. From 214 of sinus swabs, tracheal swabs, lungs, airsac, liver heart blood samples and yolk sacs of chickens suffered from respiratory distresses, 6 isolates of O. rhinotracheale were isolated. These isolates were obtained from tracheal swabs of broiler chickens aged between 28-35 days old and broiler breeder of 32 weeks old. Upon incubated on blood agar for 48 hours at 37oC in a 5% CO2 atmosphere, round, convect and grey colonies with diametres of 1-2 mm were observed. The bacteria were pleomorphic, Gram negative rods, negative catalase and positive oxidase. Biochemically, the bacteria did not change potassium nitrate, tryptophan, glucose, arginine, urea, esculin, gelatine, arabinose, mannose, mannitol, N-acetyl-glucosamine, maltose, gluconate, caprate, adipate, malate, citrate and phenyl-acetate in API 20 NE system but β –galactosidase was produced. In the API 20 NE system, the isolates were identified as 0020004, 0060004, 0020104 codes. Tracheitis, air sacculitis, pneumonia and cheesy air sacs were pathological changes generally found in chickens infected with O. rhinotracheale. Trachea is the most important organ for the isolation of O. rhinotracheale. Key Words: Ornithobacterium rhinotracheale, Infection, Chicken, Indonesia

Metabolism in compensatory growth: VI. Effect of energy yielding substrates Prapti Mahyuddin
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 1 (2002): MARCH 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An experiment was designed to investigate the effect of different rates of glucose infusion into animals fed a maintenance diet supplemented with undegraded protein (HCHO-casein). Twelve Merino wether Iambs were divided into 3 treatment groups receiving different rates of glucose infusion, 10 mmolelh, 20 mmolelh and 30 mmolelh. The intake and digestibility of dry matter were not affected by glucose infusion. The infusion of glucose increased N retention by reducing both faecal and urinary N. It was estimated that per unit of glucose infused, animal retained 0.15 g N/mmole glucoselh. The efficiency of N retention were 28%, 35% and 44% for glucose infusion rate of 10 mmolelh, 20 mmolelh and 30 mmolelh respectively. Urea entry rate decreased as glucose infusion rate increased. The estimation of protein spared by glucose infusion calculated from N retention and urinary excretion rate gave a value of 20 g and 25 g per 100 g glucose infused respectively. The proportion of glucose entry rate (GER) that could potentially derived from amino acids reduced as the rate of glucose infusion increased, being 21%, 17% and 14% for 10 mmolelh, 20 mmolelh and 30 mmolelh of glucose infused respectively. The GER, percentage of glucose oxidized and its contribution to CO2 production increased as the rate of glucose infusion increased. However, CO2 entry rate was not significantly affected by rates of glucose infusion. Glucose uptake by the hind-limb muscles increased with increasing rates of glucose infusion and strongly related with both GER and plasma insulin concentration. There was a tendency for circulating essential amino acids to reduce as the rate of glucose infusion increased, and was more prominent for branched chain amino acids (BCAA) at 30 mmolelh of glucose infusion. The reduction of amino acids in the blood circulation occurred concurrently with the reduction in plasma urea concentration and urea entry rate indicated increased net incorporation of amino acids into protein. Key words: Compensatory growth, glucose, N retention, amin acid

The effectiveness of garlic extract (Allium sativum linn) in controlling aflatoxicosis in laying hens Romsyah Maryam; Yulvian Sani; Siti Juariah; Rachmat Firmansyah; Miharja .
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 4 (2003): DECEMBER 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Aflatoxicosis is a disease generated as the consequence of aflatoxin contamination in foods and feeds. Garlic (Allium sativum Linn.), a natural spice known to reduce the toxicity of aflatoxins in poultry. Twenty five laying hens were used to study the effectiveness of garlic extract in reducing aflatoxicosis. The animals were divided into 5 groups i.e (1) control group, (2) group treated with 0.4 mg/kg BH, (3) group treated with 0.4 mg/kg BH and 4% garlic extract in feed, (4) group treated with 5 mg/kg BH, and (5) group treated with 5.0 mg/kg BH and 4% garlic extract in feed. Body weight gains, eggs production, and the activity of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), and γ-glutamytransferase (γ- GT) enzymes were observed every week. The residue levels of the aflatoxin and metabolites were measured in the eggs using high performance liquid chromatography (HPLC). The results indicated that group treated with 0.4 mg AFB1/kg BH dan 5.0 mg AFB1/kg BH resulted in decreasing body weight gains, egg production, and increasing the level of GOT, GPT and γ-GT. The addition of 4% garlic extract in the feed was effective to improve the body weight gain and egg production only in the group of chicken treated with 0.4 mg AFB1/kg BH. However, it decreased the enzymes activities of the GOT, GPT, and γ-GT, as well as reduced the aflatoxin residues and metabolites in the groups at both aflatoxin levels (0.4 and 5.0 mg/kg BH). Aflatoxin residues decreased up to 42.2% for the group treated with the low dose of AFB1 (0.4 mg/kg) and 49.0% for the group treated with the high dose of AFB1 (5 mg/kg). Key words: Garlic, aflatoxicosis, laying hens, aflatoxin residues

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