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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

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Viability of bovine demi embryo after splitting of fresh and frozen thawed embryo derived from in vitro embryo production M Imron; A Boediono; I Supriatna
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

In vivo embryo production was limited by number of donor, wide variability respond due to superovulation program and also immunoactifity of superovulation hormone (FSH). Splitting technology could be an alternative to increase the number of transferrable embryos into recipien cows. Splitting is done with cutting embryo becoming two equal pieces (called demi embrio) base on ICM orientation. The objective of this research was to determine the viability of demi embryo obtained from embryo splitting of fresh and frozen thawed embryo. The results showed that demi embryos which performed blastocoel reexpansion 3 hours after embryo splitting using fresh and frozen thawed embryos were 76.9 and 76.2% respectively. Base on existention of inner cell mass (ICM), the number of demi embryos developed with ICM from fresh and frozen thawed embryos were not significantly different (90.6 and 85.7% respectively. The cell number of demi embryo from fresh embryos splitting was not different compared with those from frozen thawed embryos (36.1 and 35.9 respectively). These finding indicated that embryo splitting can be applied to frozen thawed embryos with certain condition as well as fresh embryos. Key Words: In Vitro Embryo, Splitting, Demi Embryo, Cell Number

Molecular characteristic and pathogenicity of Indonesian H5N1 clade 2.3.2 viruses Dharmayanti NLPI; Hartawan R; Hewajuli DA; Hardiman .; Wibawa H; Pudjiatmoko .
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 2 (2013): JUNE 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The outbreak of disease in late 2012 in Indonesia caused high duck mortality. The agent of the disease was identified as H5N1 clade 2.3.2. The disease caused economic loss to the Indonesian duck farmer. The clade 2.3.2 of H5N1 virus has not previously been identified, so this study was conducted to characterize 4 of H5N1 clade 2.3.2 viruses by DNA sequencing in eight genes segment virus namely HA, NA, NS, M, PB1, PB2, PA and NP. The pathogenicity test of clade 2.3.2 viruses in ducks was compared to clade 2.1.3 viruses which predominat circulating in Indonesia. Results of phylogenetic tree analysis showed that the four of clade 2.3.2 viruses isolated in 2012 was the new introduced virus from abroad. Further analysis showed eight genes were in one group with the clade 2.3.2 viruses, especially those from VietNam and did not belong to Indonesia viruses group. The pathogenicity test in ducks showed that virus H5N1 clade 2.3.2 and clade 2.1.3 have similar clinical symptoms and pathogenicity and cause death in 75% of ducks on days 3-6 after infection. Key Words: H5N1 Virus, Clade 2.3.2, Clade 2.1.3, Phylogenetic Tree, Pathogenecity

Strategic control of acute diarrhea of newborn calves Siti Chotiah
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 3 (2012): SEPTEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Economic performance of beef cattle operations can be severely hampered by acute calfhood diarrhea. Accordingly, a study was conducted at Bbalitvet to identify the causal agents, reduce clinical incidence, and increase body weight gain of newborn calves. One potential control is application of suitable vaccines to pregnant cows. The study was begun by identifying cases of diarrhea followed by isolation and identification of the causal agents in 12 beef cattle farms in West Java. A field trial was then designed for controlling calf diarrhea in such farms. Inactive vaccines Ecoli-Closvak polivalen were administered to pregnant cows to increase specific resistance of the newborn calves. At 2 months prepartum, 12 pregnant cows were assigned either to a vaccination or a placebo group, with a booster vaccination 3 weeks prior to parturition. Strict hygenic management was provided to both groups, and all calves were provided adequately with colostrum. Subjects were observed for 5 months, starting from the time of initial vaccination until the calves were 3 months of age. In the initial farm surveys, entero-pathogenic bacteria such as Escherichia coli serotype K99 and Clostridium perfringens type A and C were isolated and identified in fecal samples from 4 beef cattle farms in 3 districts (Garut, Tasikmalaya, Ciamis) and 2 beef cattle farms in 2 districts (Tasikmalaya and Ciamis) of West Java. In the vaccination trial, good immune responses to E. coli and C. perfringens alpha toxin measured by ELISA were observed. Application of effective control of calf diarrhea including vaccination and good livestock management showed good results. No death or signs of diarrhea were found in the new born calves up to 3 months of age. The rate of body weight gain was significantly higher in calves of vaccinated dams than in calves of non-vaccinated dams. Key Words: Strategic control, Calf diarrhea

Development of ELISA technique for detecting Clostridium novyi alpha toxin Lily Natalia
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 3 (1997)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v2i3.69

the investigation of infectious necrotic hepatitis . Modification of the indirect ELISA technique in the system used is an inhibition ELISA for antigen . The test samples were liver fluid from dead animals suspected from infectious necrotic hepatitis. To analyse for alpha toxin, microtiter plates were coated with antigen or alpha toxin . The liver fluid sample thought to contain antigen or toxin was then mixed together with reference antiserum containing specific alpha antitoxin of Cl. novyi. Enzyme labelled antiglobulin was then added, followed by enzyme substrate . The difference in colour change between a reference sample containing no antigen or toxin and the test sample solution indicates the amount of antigen or toxin in the test samples . This is a competitive assay; high toxin concentrations result in less colour at the end of the test . The sandwich ELISA technique was sensitive enough to detect as little as 390 ng/ml toxin in liver fluid sample. These results indicate that the ELISA technique is useful for detecting alpha toxin of Cl. novyi and for diagnosing infectious necrotic hepatitis . Keywords: ELISA technique, Clostridium novyi alpha toxin, infectious necrotic hepatitis

Sensory quality and fatty acids composition of male local duck meat with Santoquin, vitamin E and C suplementation in ration Maijon Purba; E.B Laconi; P.P Ketaren; C.H Wijaya; P.S Hardjosworo
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Fishy odor is one of off odor attributes local duck meat. Off odor reduces consumer preference on duck meat. This experiment was conducted to evaluate effect of fish meal levels and antioxidant in diets on the off odor intensity and fatty acid composition of local duck meat. The experiment was designed based on 2x3factorial experiment. The first factor was two fish meal level: low (TIR) and high (TIT), and second factor was three antioxidant level: none, 150 ppm santoquin (Sq) + 400 IU vitamin E (VE), and 400 IU vitamin E (VE) + 250 mg vitamin C (VC)/kg diet. One hundred and twenty MA (Mojosari♂ x Alabio♀) day old male duck were allocated into six treatments: (1) TIR, (2) TIR + 150 ppm Sq + 400 IU VE, (3) TIR + 400 IU VE + 250 mg VC (4) TIT, (5) TIT + 150 ppm Sq + 400 IU VE, and (6) TIT + 400 IU VE + 250 mg VC. Off odor intensity on raw and boiled meat, and fatty acid composition in boiled duck meat were used as parameters. The result indicated that fish meal levels did not significantly (P>0.05) affect the off odor intensity in raw and boiled meat. However, antioxidants supplementation significantly (P<0.05) reduced off odor intensity in both raw and boiled meat. Antioxidants supplementation in diet prevented the unsaturated fatty acids from lipid oxidation in boiled meat. This experiment suggests that supplementation of 150 ppm santoquin + 400 IU vitamin E or 400 IU vitamin E + 250 mg vitamin C to the duck diets were effective in reducing off odor and hence increased the sensory quality of duck meat. Key words: Local Duck, Antioxidants, Fish Meal, Fatty Acids, Quality Sensory

icacy test of hexane extract of Annona squamosa L seeds for Chrysomya bezziana larvae growth in vitro April H Wardhana; E Widyastuti; A.W.A Wiratmana; S Muharsini; Darmono .
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 4 (2004): DECEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Chrysomya bezziana is primer agent causing myiasis in livestock and human throughout Africa to Asia. At present, treatment of myiasis using chemical synthetic insecticide causing environment and livestock production contaminations. The aim of this study was to evaluate the effect of hexane extract of Annona squamosa L seed to kill C. bezziana as botanical insecticide. Three level and method preparation of larvae (L1, L2 and L3) were carried out. Meat blood mixture (MBM) and larval rearing media (LRM) were mixed with hexane extract of A. squamosa L seed and tested to 625 and 750 larvae (L1and L2 respectively), while for L3 625 larvae were soaked in the solution containing the hexane extract. This last preparation was carried out to observe the effect of toxic contact. Each larvae treatment was divided into 5 levels such as negative control using distillation water (PO), given 0.25% hexane extract (P I); 0.50% (P II); 0.75% (P III) and positive control was given with 0.10% Asuntol®. This assay was to know digest toxic effect of hexane extract of A. squamosa L seed for those larvae. Number of 625 instar III larvae (L3) was soaked in the solution containing the hexane extract of A. squamosa L seed for 10 second, placed into vermicullite and incubated on 36oC. This assay was to know contact toxic effect of them. All of larvae were allowed to become pupae. Parameters oberserve were number of larvae death, pupae weight and number of pupae become into fly. The data was analyzed using Anova (5%) and Z test (5%) then smallest significant difference test (BNT 5%). The results showed that 0.50% of hexane extract of A. squamosa L seed was able to decrease pupae weight for L1 and L2 and to cause fail pupae become fly (P<0.05). Instar III larvae (L3) soaked in PI until P III and not effect to pupae weight, pupae become fly and survival of adult flies (P>0.05). Key words: Chrysomya bezziana, myasis, srikaya, A. squamosa L, in vitro

Vaccination against Chrysomya bezziana: a summary of current knowledge Peter Willadseni
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 3 (2000): SEPTEMBER 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Methods for the culture of the Old World Screwworm Fly Chrysomya bezziana on a small laboratory scale, the production of life stages and tissues suitable for vaccine study, and the in vitro and in vivo assessment of vaccine effects have all been developed. These methods would be applicable to further studies on screwworm control by many alternative technologies. It has been shown to be possible to induce dramatic immunological effects on feeding larvae and to isolate and characterise individual antigens. However, a recombinant vaccine remains elusive. Current understanding of the effects of vaccination is discussed and suggestions made for possible future research directions. Key words: Chrysomya bezziana, Old World screwworm fly, vaccine, recombinant antigen, sheep

Responses of Giving n-Hexane Jaloh Extract on Broiler Chicken which Heat Stress Condition to Expression of iNOS Enzyme in Lung Tissue, Serum level of Glucose and Calcium Sugito Sugito
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Giving of extract n-hexane of jaloh bark (EHJ) can lessen impact heat stress on broiler chicken. The studies of activity of EHJ in lessening heat stress impact at broiler chicken have not been conducted yet. An experiment was conducted to study the effect of extract n-hexane of jaloh bark (EHJ) administration on body temperature, level of calcium and glucose in serum, inducible nitric oxide synthase (iNOS) in lung tissue, and behaviors of broilers exposed to heat stress at temperature 33 ± 1oC. This study used 18 broilers aged 20 days which were divided into 3 groups. The first group was broilers given heat stress without EHJ (CP). The second group was treated heat stress and EHJ at dose 10 mg/kg BW (CP+EHJ). The EHJ was given 1 hour before cage temperature was raised. Broilers in group CP and CP+EHJ were divided into 3 periods of slaughtering i.e. 2 and 4 hours after given heat stress and 2 hours after heat stress termination. Each of slaughtering period consisted of 3 broilers as replication. Result of this research indicated that CP+EHJ lessen stress in the form of restlessness and increased expression of iNOS in the lung tissue. It was assumed that one of mechanism of EHJ in lessening stress on broiler related to forming of iNOS enzyme in the lung tissue. Key Words: Salix, Heat Stress, Calcium, Glucose, i-NOS Enzyme

Nutrient digestibility and beef cattle performance fed by lerak (Sapindus rarak) meal in concentrate ration S. Suharti; D.A. Astuti; E. Wina
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 3 (2009): SEPTEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

This research was aimed to study the use of Lerak fruit meal to improve performance and feed digestibility of beef cattle. The research consisted of two trials (in vitro and in vivo studies). The in vitro trial was screening of bioactive compounds (saponin, tanin, dan diosgenin) in Lerak fruit (including seed) and continued to evaluate the effectivity of these compounds against ruminal protozoa. The in vivo study was done using 12 Ongole Crossbreed cattle which received 1of 3 different treatments: 1) concentrate without Lerak as control, 2) concentrate containing 2.5% Lerak, and 3) concentrate containing 5% Lerak. Anti protozoal activity, daily gain, and nutrient digestibility of beef cattle were measured. Results showed that saponin concentration in Lerak extracted by methanol was higher than that in Lerak extracted by water and Lerak meal, 81.5%; 8.2% and 3.85% respectively. Lerak extracted by methanol have higher antiprotozoal activity in vitro than Lerak extracted by water. In vivo experiment showed that there were no significant differences (P>0.05) of nutrient intake and digestibility in all treatments, that means the ration had good palatability and quality. Average daily gain of PO fed 2.5% Lerak was 20% higher than that of control diet (0.9 kg/day). Key words: Sapindus rarak, Protozoa, Performance, Ongole Crossbreed, Digestibility

The effect of feeding pre-starter on performance efficiency of local chicken (KUB chicken) Sofjan Iskandar; Cecep Hidayat; T. Cahyaningsih
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 3 (2014): SEPTEMBER 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An experiment in feeding pre-starter diets was carried out on local chicken (KUB chicken) raised to the age of 84 days. Four hundred and eighty day-old KUB chicks were allocated to experimental diets of P1 = standard diet without pre-starter; P2 = OASIS® pre-starter for 48 hours feeding; P3 = COBA-1, a mixture of 76.3% yolk powder, 0.76% inulin powder, 7.63 % honey and 15.3% tomato sauce, for 24 hours feeding; P4 = P3 given for 48 hours feeding; P5 = fresh papaya for 24 hours feeding; P6 = P5 for 48 hours feeding; P7 = fasting for 24 hours and P8 = fasting for 48 hours. Following treatment, the chicks were then fed with standard diet, containing 17.5 % crude protein with 2800 kcal ME/kg up to the end of the experiment. Results showed that the group of chicken on pre-starter diet of ripe papaya fruit (P5 and P6), responded better EPEF (European Performance Efficiency Factor) value of 442 and 356 g/bird, respectively in chicken of P5 and P6. This better response was due to particularly higher viability and the efficiency in utilization of feed.

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Home Page

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

Abstract

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Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA