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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

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Effects of hormonehCG following injection of estnanate of reproductive performances ofswamp buffalo (Bubalus bubalis) Polmer Situmorang; Abdul Rachman Siregar
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 4 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Low reproductive performance of buffalo resulted the population growth in Indonesia was only slightly increase during the last 50 year. Reproductive performance can be improved by hormone treatment during synchronization . One study was conducted in Porsea and Siborong-borong to see the effects of hormone hCG on the reproductive performances of swamp buffalo . A total of 34 buffaloes were kept in field grazing and commercial concentrate 4 kg/head/day was given for supplementation in 27 buffaloes and the rest 7 buffaloes treated as control with no supplemeniation . Synchronization performed by two intromuscular (IM) injections of2 ml estrumate in 11 days interval . The buffaloes were divided into 2 groups of 16 buffaloes without hCG (Control group) and 18 buffaloes with hCG (Treated group). Treated group wereinjected with 500 IU hCG intro-muscularly (IM) 24-48 hours following second injection of estrumate . All buffaloes were artificially inseminated (AI) 48 and 72 hours after the second injection of estrumate. Results showed that injection of estrurnate twice in 11 days interval was very effective to synchronize oestrus of buffaloes. The persentage of buffalo showing oestrus was 67 .5 and 85 .3% following 48 and 72 Ins of thesecond injection of estrumate resfectively . The effectiveness of estrumate was significantly affected by body condition of buffaloes where the persentage of oestrus 48 and 72 hours following estrumate injection was significantly higher (P<0.01) in buffaloes with good body condition than those in poor body condition (74.5 and 92.5% vs 42 .9 and 57 .1%). Injection ofhCG following estrumate increased the percentage of oestrus and pregnancy of buffalo. Percentages of oestrus of buffaloes 48 and 72 hours following estrumate injections was significantly higher (P<0 .01) in hCG treatment (83 .3 and 100%) than those in control groups (68 .8 and 81 .3%) . The pregnancy rate of buffalo with good body condition was significantly higher (P<0.01) in group of buffaloes with hCG than those control groups ( 86 .6 vs 50.0%) . It is concluded that injection of hCG following estrumate can improve the reproductive performance ofswamp buffalo Keywords : Swamp buffaloes, hCG, oestrus, body condition

Optimizing artificial insemination on swamp buffalo (Bubalus bubalis) through synchronization of estrus and ovulation Riasari Gail Sianturi; B Purwantara; I Supriatna; Amrozi .; P Situmorang
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 2 (2012): JUNE 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Artificial insemination (AI) program in swamp buffalo will be more efficient by implementing synchronization of estrus and ovulation. By synchronizing of ovulation, AI can be done at a fixed time schedule without concerning to estrus detection. Gonadotropin Releasing Hormone (GnRH) and human chorionic gonadotropin (hCG) have been used in protocols of estrus synchronization to induce ovulation. A study of AI in swamp buffalo was conducted on 83 buffaloes to evaluate the impact of protocol of estrus synchronization on reproductive efficiency of swamp buffalo. The three protocols used were Ovsynch (GnRH-PGF2α-GnRH-AI), convensional (PGF2a-PGF2a-AI) and Select-Synch (GnRH-PGF2a-AI). Inducing of ovulation were done by administration of GnRH or hCG after prostaglandin (PGF2α) injection. AI was done at 18 and 24 hour after the second GnRH injection (66 hours and 72 hours after PGF2α injection) for Ovsynch method and 72 hours after the last PGF2α injection for convensional and Select-Synch methods. Parameters observed were percentage of estrus and pregnancy from the three estrus synchronization protocols and the differences were analysed by statistics. All of buffaloes (100%) in the three synchronization protocols showed estrus behavior prior to AI. The percentage of pregnancy was 64.71; 77.14 and 83.87% for the Ovsynch, convensional and Select-synch respectively and there was no significantly different (P > 0.05) among the three protocols. hCG administration after the last PGF2α also did not affect pregnancy rate, ie: 76.47 vs 77.78% (with hCG vs without hCG) for the convensional and 88.24 vs 78.57% for the Select-Synch. It is concluded that the synchronization of estrus protocols in this study can synchronize the estrous and ovulation and AI can be done in a fixed-timed and could reach better pregnancy rate of swamp buffalo. Key Words: Swamp Buffalo, Synchronization, Estrus, Ovulation, AI

Mutation detection on isotype-1 β tubulin genes of Haemonchus contortus resistant strain to benzimidazole using single strand conformation polymorphism Dyah Haryuningtyas
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 3 (2005): SEPTEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Evidence of anthelmintic resistance of Haemonchus contortus to benzimidazole groups based on Larval development assay (LDA) and Fecal egg count reduction test (FECRT) test has been reported in some areas in Indonesia. Studies on sheep parasite H. contortus have shown that resistance to benzimidazole drugs is correlated with selection for individuals in the population possesing a spesific isotype-1 β tubulin gene. The aim of this study was to determine mutation on central part of isotype-1 β tubulin gene of benzimidazole resistant strain H. contortus using Single Strand Conformation Polymorphism (SSCP). H. contortus worms were isolated from four sheep from two government farms that resistance to benzimidazole have been occurred (SPTD Trijaya, Kuningan, West Java and UPTD Pelayanan Kesehatan Hewan, Bantul, Yogyakarta) and one sheep that susceptible from Cicurug, Bogor, West Java as a kontrol. Resistance status to benzimidazole was reexamined individually with LDA and FECRT before sheep slaughtered. DNA was extracted from female H. contortus worms. A fragment of 520 bp isotype- 1 β tubulin gene was amplified using Polymerase chain reaction (PCR) and then analyze using SSCP. The results showed that there were polymorphism in isotype-1 β tubulin gene among H. contortus susceptible (Cicurug, Bogor, Jawa Barat) and two H. contortus resistant strains from SPTD Trijaya, Kuningan, West Java and UPTD Pelayanan Kesehatan Hewan, Bantul, Yogyakarta. Mutation occurred in the different nucleotide of the two resistant strain. Key Words: Haemonchus contortus, Benzimidazole, Isotype-1 β tubulin gene, SSCP

Utilization of palm oil sludge in poultry diet. 1. Dried palm oil sludge and its fermented product in broiler’s diet A.P Sinurat; T Purwadaria; P.P Ketaren; D Zainuddin; I.P Kompiang
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 2 (2000): JUNE 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Palm oil sludge a by product of palm oil industry is not commonly used in poultry feed due to some limiting factors such as low protein and amino acids content and high fiber content. These limiting factors were expected to be reduced by fermentation technology. Therefore an experiment was conducted to study the use of fermented (FLS) and non-fermented palm oil sludge (LS) for broiler chicken feed. Two hundred and ten day-old broiler chicks were used for this study. The birds were allocated into 35 cages with 6 birds in each cage. Each 5 group of birds were fed with one of 7 experimental diets. All diets were formulated with similar nutrient contents containing either LS or FLS at 3 different levels (5, 10 and 15%) and a control diet with no LS or FLS. The experimental diets were fed for 6 weeks and the performances were observed. Carcass yield, abdominal fat, weight of liver and gizzard were also measured at the end of the trial. The results showed that LS or FLS can be included in broiler’s diet, since the mortality, carcass yield, abdominal fat, liver and gizzard were not significantly affected. Best level inclusion of LS was 5%. Although inclusion of 10-15% LS did not affect growth and feed conversion significantly, the feed intake was significantly depressed. The best level inclusion of FLS was 10%, since higher level (15%) caused growth depression. Key words: Palm oil sludge, fermentation, broilers

Antibacterial activity and retained protein of earthworm meal (Lumbricus rubellus) as feed additive combined with chitosan Ahmad Sofyan; Ema Damayanti; Hardi Julendra
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

This research was conducted to enhance the bacterial growth inhibition of E. coli by using earthworm (Lumbricus rubellus) meal (TCT) which was added with chitosan and its effect on the retained protein in broilers. Inhibition of E. coli growth was tested using dilution method on the nutrient broth by additional 2% TCT combined with 0, 0.5, 1.0 and 1.5% chitosan. Retained protein was measured using broiler fed diet containing 2% TCT (w/w) and added by chitosan 0, 0.5, 1.0 and 1.50% of TCT (w/w). The numbers of 15 broilers Cobb strain 35 days old were arranged on Completely Randomized Design (CRD). Results showed inhibition of E. coli was increased using TCT mixed chitosan. The highest inhibition to E. coli growth obtained from TCT + 0.5% chitosan. Retained protein tended to increase up to 1% (w/w) chitosan. Otherwise, chitosan level more than 1% could reduce protein retention. It is concluded that use of 1% chitosan increased TCT capability to inhibit E. coli and protein retention in the broilers. Key Words: Feed Additive, Chitosan, L. rubellus, E. coli

Carcass percentage and quality of broilers given a ration containing probiotics and prebiotics Muhammad Daud; Wiranda G Piliang; I. Putu Kompiang
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 3 (2007): SEPTEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Probiotics is a feed additive in the form of life microorganisms that balance microorganism population in the digestive tract. While prebiotics is a feed substance which is not digested, and selectively improves growth and activity of useful microbes in large intestine. The objectives of this research were to study the carcass percentage and carcass quality of broilers given a ration containing probiotics and prebiotics. Four hundred eighty day-old chicks of broiler Arbor Acres strain were divided into four dietary treatments and three replications (40 birds / replicate). Ration used was consisted of: R1 = basal ration + 0.01% antibiotics (Zinc bacitracin), R2 = basal ration + 0.2% probiotics (Bacillus spp), R3 = basal ration + 0.2% probiotics + 0.5% prebiotics and R4 = basal ration + 0.5% prebiotics (katuk leaves). The variables observed were: carcass percentage, fat content in the abdomen, liver and carcass, cholesterol content in the liver, carcass and blood serum. The results showed that the carcass quality of broiler received probiotics and prebiotics either independently or the combination was significantly (P<0.05) different. The fat content of liver and thigh, and breast cholesterol of R3 was significantly (P<0.05) lower than that of the control (R1). It is concluded that combination of probiotics and prebiotics were able to decrease the carcass fat and cholesterol content at six week of age. Key Words: Probiotics, Prebiotics, Carcass, Broilers

SDR-2 as a strong candidate vaccine for brucellosis in animals Maxs U.E Sanam
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 3 (2001): SEPTEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Various mutant strains of Brucella suis type-1 have been recently developed including SDR-2 and SD-7. This research was aimed at revealing the course of infection and serological reactions, as well as the protection capacity of these mutant strainscompared to the reference vaccine strain 19, and the virulent strain B. suis type-1 in Quackenbush mice as a model. Antibody reactions were measured by an Enzyme-linked Immunosorbent Assay (ELISA) and degree of infection was determined by bacterial spleen count. The results showed that the SD-7 was unable to perform infection in mice. Whereas SDR-2, strain 19 and the virulent B. suis type-1 were able to colonize the mice spleens with varying rate of infections. The inoculation of SDR-2 to the mice produced mild infection and lasted shorter than the virulent strain even with the reference vaccine strain 19. The number of SDR-2 and strain 19 organisms were sharply dropped at week-12 post inoculation while that of virulent strain was significantly remained high. Serological responses induced by SDR-2 was the lowest followed by those of strain 19, and the virulent strain. On the challenge with a virulent B. suis, histological examinations of the spleen of the control mice revealed that there was a marked depletion of lymphoid cells and reticuloendothelial hyperplasia in lymphoid follicles. However no significant pathological changes were observed in groups inoculated with either SDR-2 or Strain 19. Enumeration of survival challenge organisms in the spleens clearly demonstrated that SDR-2 provided significant protection (2.17 Log10) to the animals which was comparable to that provided by strain-19 (2.20 Log10). In conclusion, SDR-2 has a potential as a vaccine for use in pigs against Brucella suis infection. Furthermore SDR-2 offers some advantages over strain 19 in that it is less virulent and induces less antibody responses than the strain 19 and thus may have application in other animals. However, furher study on its efficacy as a vaccine for brucellosis in pigs as the primary host for B. suis needs to be assessed. Key words: Brucella suis, mutant strains, SDR-2 vaccine, strain 19, antibody

The effect of “patikan kebo”(Euphorbia hirta L) preparations on erythrocyte number, haemoglobin level and haematocrit value of chicken infected with Eimeria tenella April H Wardhana; E Kencanawati; Nurmawati .; Rahmaweni .; C.B Jatmiko
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 2 (2001): JUNE 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Patikan kebo” (Euphorbia hirta L) is a medicinal plant mostly used to treat gastro-intestinal track problems empirically. The aim of this study was to determine the effect of distillation, infusion and extract of “patikan kebo” (Euphorbia hirta L) preparations on erythrocyte number, haemoglobin level and haematocrit value in chickens that were infected by Eimeria tenella. Twenty four Hubbard strain broiler chicken raised from one-day-old until three-week-old were used for this study and divided randomly into 4 groups: P 0 (control), P I (distillation treatment), P II (infusion treatment), and P III (extract treatment). A total of 5.000 oocyst of E. tenella were infected into each chicken and the dose of Euphorbia hirta L was 500 mg/kg Wb. A completely randomized design was used and the data were tested by ANOVA. The result indicated that distillation, infusion and extract preparations of Euphorbia hirta L had the potency to increase erythrocyte number, haemoglobin level and haematocrit value. Key word : Eimeria tenella, Euphorbia hirta L, erythrocyte, haemaoglobin, haematocrit

Development of a nested PCR for detection of Bovine herpesvirus-1 (BHV-1) in bovine nasal secretion and semen Muharam Saepulloh; R.M Abdul Adjid; I Wayan T. Wibawan; Darminto .
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A nested polymerase chain reaction (nPCR) assay for detection of Bovine herpesvirus-1 (BHV-1) in bovine semen and nasal secretions was successfully developed. The nested Polymerase Chain Reaction was based on external and internal primers from the viral gD glycoprotein gene. This nPCR assay was 1000-fold more sensitive than using PCR external primer. The nested PCR has a detection limit as low as 5 ag/ml pure BHV-1 DNA and 100,75 TCID50/500 mL BHV-1 infected cells. On the other hand, PCR using external primer had detection limit of about 5 fg/ml pure BHV-1 DNA. Specificity studies showed that nPCR could only detect BHV-1, whereas BHV-4, PRV, PI-3 and BRSV can not be detected. In addition, nPCR was also capable in detecting BHV-1 in nasal secretion samples from animal without clinical signs. A total of 405 samples consisted of 381 nasal secretion and 24 fresh semen samples have been tested with the nPCR. The result revealed that from 381 nasal secretion samples tested, 14 samples showed to be positive (3.68%), consisting of 13 out of 294 (4.42%) nasal secretion samples collected from Pangalengan West Java, and 1 out of 87 (1.54%) samples collected from Bogor. Furthermore, 2 out of 11 (18.18%) extended semen samples collected from Bogor and 2 out of 13 (15.38%) fresh semen samples collected from Pasuruan also showed to be positive. In addition, the nPCR was faster and easier to perform than the standard viral isolation test. It is concluded that, the nPCR can be used as test of choice for routine diagnosis of BHV-1. Key Words: Nested PCR, BHV-1, Semen, Glycoprotein D Gene, TCID50

Milk production capacity of priangan prolific sheep: II. The lactation curve B Tiesnamurti; I Inounu; Subandriyo .; H Martojo
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 1 (2003): MARCH 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A calculation was made for the lactation curve of Priangan sheep using Wood equation to find out how much differences actual milk production of uncorrected ewes compared to those corrected using parity and litter size born. Milk production was measured using weighing of the lambs before and shortly after suckling, with average of daily and total milk production were 519.5 g/head and 43.6 kg head-1 lactation-1, respectively. The average of parameter a, which reflected milk production at the beginning of the lactation period was 6.296 that equivalent to 571.5 g/head and significantly affected (P<0.05) by parity. The b parameter which reflected the rate of milk increment at the beginning of the lactation period was found to be 0.528 and significantly affected (P<0.05) by parity. The c parameter which reflected the rate of milk decline at the end of lactation period was found to be -0.20 and significantly affected (P<0.05) by parity. The average persistency which reflected the duration of maximum milk production was found to be 22.58 days without any significant contribution of parity and the number of lamb born. The average time to reach maximum milk production was found to be at week 3.5 after lambing without any significant contribution of parity and litter size. The average estimation of maximum milk production was 708.4 g/head and significantly affected (P<0.05) by ewe parity. Key words: Milk production, lactation curve, Priangan sheep

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Ahmadi Riyanto

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medpub@litbang.deptan.go.id

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ahmadi_puslitbangnak@yahoo.com

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Kota bogor,

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