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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

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Astudy on the pathogenicity of Eimeria tenella in village chickens in South Kalimantan Hamdan, Salfina A; Siswansyah, D.D
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 4 (1998)
Publisher : Indonesian Animal Sciences Society

A study on the pathogenicity of various isolates of coccidia (Eimeria tenella) was carved out in village chickens . The isolates were collected from different districts, namely Hulu Sungai Selatan (HSS), Hulu Sungai Tengah (HST), Tapin, and Tanah Laut, South Kalimantan . The result showed that the pathogenicity of E. tenella isolated from two weeks old chickens in HSS district was higher than isolates from the other three districts of the same age of chickens . Further study showed that the isolates of E. tenella from HSS district appeared to cause mortality rates of 64 .3%, 42 .9%, 28 .6%, and 7 .1%, and caecal lesions scores of +4, +3, +2, and +2, in 1, 2, 3, and 4 months old chickens respectively . The body weight gains of the four different age groups of chickens infected with 200,000 oocysts were very significantly lower (P<0.01) than the control group. Â Keywords: Pathogenicity, Eimeria tenella, village chicken, South Kalimantan

Improvement of nutritive value of acacia mangium bark by alkali treatment Wina, Elizabeth; Toharmat, Toto; Astuti, Widia
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 3 (2001)
Publisher : Indonesian Animal Sciences Society

Bark, especially from Acacia mangium is a by-product from wood processing industries that commonly found in Indonesiaand in big amount will cause environmental problems. One of the alternatives to utilize bark is for animal feed. The aims of this experiment are to improve the nutritive value of bark by alkali treatments (urea and sodium hydroxide) and to determine the level of substitution of elephant grass by bark. The experiment consisted of 3 in vitro studies and 1 in sacco study. In vitro studies consisted of 1) the use of urea or NaOH by wetting and incubation-method, 2) the use of different concentration of Na OH (0-4%) by soaking method, 3) determination of substitution level of elephant grass by treated bark. In sacco study was conducted at 0, 6, 12, 24, 48 and 72 hours of incubation to compare the degradation of treated bark to elephant grass. The results show that urea treatment did not improve DM or OM digestibilities of bark. Soaking bark in 4% NaOH solution was more effective than wetting and incubation-method in improving in vitro digestibility. (49.26% vs19.56% for soaking and dry-method, respectively). In sacco studyl shows that treated bark had a very high solubility at 0 hour incubation but the degradation at 72 hours incubation was not significantly different from that of 0 hour incubation. The gas produced at in vitro study of treated bark was very low indicated that there was no degradation of bark at all. The level of substitution of elephant grass by treated bark up to 30% gave a non-significant digestibility value to that of 100% elephant grass. In conclusion, bark after tannin-extraction was a better feedstuff for animal feed. The soaking method in 4% NaOH solution improved the digestibility of bark significantly and the level of substitution of elephant grass by treated bark was 30%. Â Key words: Bark, acacia mangium, urea, sodium hydroxide, digestibility

Metabolism in compensatory growth. V. Effect of undegraded protein in compensatory growth Mahyudin, Prappti
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 3 (2001)
Publisher : Indonesian Animal Sciences Society

An experiment was designed to study the effect of increasing availability of amino acids in growing animal fed maintenance diet and which previously subjected to underfeeding. Twelve wether Iambs were divided into 3 treatment groups, each was fed pelleted lucerne (Medicago sativa). The treatments were: diet at maintenance energy level (M), M + 60 g formaldehyde treatedcasein(M + HCHO-casein) and ad libitum. The increase in protein consumption increased nitrogen (N) retention, although the highest efficiency of N retention occurred in animal fed M diet (0.36) compared to those fed M + HCHO-casein (0.31) or ad libitum diet (0.2). Provision of amino acids by supplementation of 60 g HCHO-casein resulted in an increment of 19 g glucose/d or 32 g glucose/lOO g protein. Glucose entry rate (GER) increased with increasing digestible crude protein. Although GER was not different between animals on M and M + HCHO-casein diet, the uptake of glucose in the hind-limb muscles of animals on the M + HCHO-casein was twice (0. 18mM) than that of animals on the M diet (0.08 mM). There was a significant effect on the uptake and output of essential amino acids, leucine, isoleucine, lysine and threonine and non-essential amino acids, tyrosine and glutamine as levels of protein in the diet increased. Supplementation with HCHO-casein increased the arterial blood concentration of branch chain amino acids (BCAA) by 76 % and phenylalanine by 61 %. In general there was an increase in the arterial concentration of amino acids in animals fed either M + HCHO-casein or ad libitum. However, this increase was followed by increased amino acids oxidation, which showed in increased urea excretion. There was a positive correlation between urinary urea and N intake, suggesting that amino acids were not fully utilized for protein synthesis or protein deposition. Â Key words: Compensatory growth, amino acid, N retension

Intake and digestibility of untreated and urea treated rice straw base diet fed to sheep Yulistiani, D; Gallagher, J.R; Barneveld, R.J. Van
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 1 (2003)
Publisher : Indonesian Animal Sciences Society

Rice straw as one of agricultural by-products has low quality due to low content of essensial nutrients like protein, energy, minerals and vitamin as well as poor palatability and digestibility. Therefore, the quality of rice straw needs to be improved in order to increase its utilization by gastrointestinal tract of ruminants. The purpose of this study is to compare untreated and urea treated rice straw as basal diets for sheep. Twelve mature Merino wethers (average body weight 53.62 + 3.44 kg) were separated into 4 groups based on their live weight with each groups assigned three diets, that are: diet 1 untreated rice straw with high forage legume content, diet 2 urea ensiled rice straw and diet 3 rice straw sprayed with urea solution at feeding time. Diets were allocated based on a randomized complete block design. Urea ensiled rice straw was prepared by spraying chopped straw with urea solution to yield straw containing 4% urea and 40% moisture, then kept in air tight polythylene bags for 6 weeks. The untreated, ensiled and urea supplemented rice straw were mixed with other feed ingredients to provide isoenergetic and isonitrogenous diets. Diets were formulated to meet maintenance requirement according to NRC. Sheep were adapted to experimental diets for 15 days, and after adaptation period, a metabolism trial was conducted. Results reveal that dry matter intake permetabolic body weight (DMI/W0.75), DE (digestible energi) intake and apparent digestibility of NDF (neutral detergent fibre) were not significantly different between diet 1 and diet 2. Apparent digestibility of DM (dry matter), OM (organic matter), and ADF (acid detergent fibre), as well as N retention were not significantly different between three diets. Positive result in N retention was only observed in diet 2, while others were negative. It may be concluded from this study that untreated rice straw basal diet supplemented with forage legume offer an alternative method other than urea ensiled for improving the nutritional value of rice straw as a ruminant feed on small farmer. Â Key words: Rice straw, urea treated, digestibility, and sheep

Successful ICSI depends on DNA stability and disulfide status of sperm nuclei Said, Syahruddin; Niwa, Koji
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 3 (2004)
Publisher : Indonesian Animal Sciences Society

Relation of successful ICSI (intracytoplasmic sperm injection) with DNA stability and thiol-disulfide status of rat spermatozoa nuclei was investigated. Testicular, caput and cauda epididymal spermatozoa have been used for this research. The nuclei of these spermatozoa were stained with aeridine orange for DNA stability test and labeled with mBBr for thiol-disulfide status before injesting into oocytes. Cauda epididymal spermatozoa were very stable and contained high disulfide than caput epididymal and testicular spermatozoa. Results from spermatozoa injection into oocytes show that they were capable to fertilize oocytes. However, testicular spermatozoa can not develop up to blastocyst stage. These results indicated that successful ICSI at least in the rat depends on DNA stability and disulfide status of sperm nuclei. Â Key words: ICSI, DNA stability, disulfide status, acridine orange, mBBr

Mutation detection on isotype-1 Î² tubulin genes of Haemonchus contortus resistant strain to benzimidazole using single strand conformation polymorphism Haryuningtyas, Dyah
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 3 (2005)
Publisher : Indonesian Animal Sciences Society

Evidence of anthelmintic resistance of Haemonchus contortus to benzimidazole groups based on Larval development assay (LDA) and Fecal egg count reduction test (FECRT) test has been reported in some areas in Indonesia. Studies on sheep parasite H. contortus have shown that resistance to benzimidazole drugs is correlated with selection for individuals in the population possesing a spesific isotype-1 Î² tubulin gene. The aim of this study was to determine mutation on central part of isotype-1 Î² tubulin gene of benzimidazole resistant strain H. contortus using Single Strand Conformation Polymorphism (SSCP). H. contortus worms were isolated from four sheep from two government farms that resistance to benzimidazole have been occurred (SPTD Trijaya, Kuningan, West Java and UPTD Pelayanan Kesehatan Hewan, Bantul, Yogyakarta) and one sheep that susceptible from Cicurug, Bogor, West Java as a kontrol. Resistance status to benzimidazole was reexamined individually with LDA and FECRT before sheep slaughtered. DNA was extracted from female H. contortus worms. A fragment of 520 bp isotype- 1 Î² tubulin gene was amplified using Polymerase chain reaction (PCR) and then analyze using SSCP. The results showed that there were polymorphism in isotype-1 Î² tubulin gene among H. contortus susceptible (Cicurug, Bogor, Jawa Barat) and two H. contortus resistant strains from SPTD Trijaya, Kuningan, West Java and UPTD Pelayanan Kesehatan Hewan, Bantul, Yogyakarta. Mutation occurred in the different nucleotide of the two resistant strain. Â Â Key Words: Haemonchus contortus, Benzimidazole, Isotype-1 Î² tubulin gene, SSCP

Pre-weaning growth of Boer x Peranakan Etawah goats Kostaman, T; Sutama, I-K
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 2 (2005)
Publisher : Indonesian Animal Sciences Society

An experiment was conducted to study the growth patterns of Boer x Peranakan Etawah (PE) crossbred during pre-weaning period, at the Indonesian Research Institute for Animal Production, Ciawi-Bogor. Sixty-one kids were used in this experiment. They were reared with their mothers in group pen (3 x 4 m), with each pen contained 6-7 does. Does were fed of 2.5 kg freshlychopped King Grass and 0.7 kg concentrate head-1 day-1. Research results showed that birth weight of Boer x PE kids (group A) were significantly higher than those of PE kids (group B) (4.29 Â± 0.63 vs 3.71 Â± 0.89 kg/head, P<0.05). However, average preweaning daily weight gain (ADG) (116.40 Â± 49.95 vs 105.29 Â± 28.36 g/head, P>0.05) and weaning weight (14.64 Â± 4.56 vs 13.30 Â± 2.71 kg/head, P>0.05) were not significantly different between the groups. Pre-weaning mortality of the kids was relatively high in both group A (25%) and B (21,21%). While sex ratio (male : female) was 57.14 : 42.86% in group A and 51,52 : 48,48% in group B. Â Â Key Words: Growth, PE, Boer Goat, Pre-Weaning

The effect of kinds and concentration of cryoprotectant and thawing methods on frozen semen of Arab chicken Iskandar, Sofjan; Mardalestari, Rufika; Hernawati, Resmi; Mardiah, Enok; Wahyu, Endang
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 1 (2006)
Publisher : Indonesian Animal Sciences Society

The success of freezing chicken semen is the hope for preserving Indonesian native chickens. Semen from twenty Arab roosters were collected using massage technique once in a week. Cryoprotectant DMA (dimethyl acetamide) or DMF (dimethyl formalmide) of 7 or 9% and thawing A at temperature of 30oC for 30 seconds or in B at 5oC for 5 minutes. The volume of fresh semen was 0.3 Â± 0.072 ml/ejaculate, white colour, rather thick to thick, with 2200 Â± 372 millions sperms/ml and pH 6.95 Â± 0.32, 4+/3+ mass movement, 80% motility, 84 Â± 4.48% and abnormality of 14.75 Â± 1.28%. There were not statistically significant (P>0.05) effect of interaction of treatments (kinds and concentrations of cryoprotectant, and thawing methods) on motility and live-sperms. Sperm motility preserved with DMA (34.69%) significantly higher than with DMF (29.84%). Sperm motility was also significantly higher (34.53%) when preserved with 7% cryoprotectant than with 9% (30%). Thawing-A significantly gave higher motility (35.31%) than thawing-B did (29.22%). Live-sperms of semen preserved with DMA (46.75%) was significantly higher than with DMF (41.72%). Cryoprotectant concentration of 7% gave higher live-sperms (46.98%) than of 9% did (41.48%). Thawing-A left live-sperms of 47.14%, which was significantly higher than thawing-B did (41.30%). Key Words: Frozen Semen, Arab Rooster, Cryoprotectants, Thawing Methods

Identification and characterisation of heat-stable allergens from Sarcoptes scabiei Tarigan, Simson
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 1 (2006)
Publisher : Indonesian Animal Sciences Society

Animals or human recovered from Sarcoptes scabiei infestation acquired protective immunity against reinfestation. The protective immunity is considered to be associated with a type-1-hypersensitivity reaction against allergens instigated by the mites during infestation. It is assumed that these allergens have the potential to be used as the main component of an anti-scabies vaccine. The purpose of this study is to identify and characterise the sarcoptic allergens. For this purpose, 645 mg of mites, collected from mangy goats, were homogenised in PBS to prepare soluble mite proteins. Fractionation of proteins was initially performed on a Q-sepharose column but the results were unsatisfactory. Consequently, SDS PAGE was used as an alternative. Proteins from the gel were transferred onto a nitrocellulose membrane. The membrane was cut into strips so each strip contained proteins with molecular weights of Â³ 90, 80-90, 70-80, 60-70, 50-60, 40-50, 30-40, 25-30, 20-25, 15-20 and 10-15 kDa, respectively. The heat stability of the allergens was determined by heating the suspension at 60ÂºC for 60 minutes, whereas their dialysability was evaluated using a 10-kDa-cut-off ultramembrane. The activity of the allergens was assayed by an intradermal test on sensitised goats. This study showed that mite protein extract was very potent allergens since mite extract containing as little as 1 ng mite proteins still caused an obvious hypersensitive reaction. The mite extract contained heat-stable, dialysable and non-dialysable allergens. All fractions recovered from a Q-sepharose column contained allergens with almost equal potency. Fractionation with the SDS-PAGE revealed that the allergens had molecular weights of 35 and <10 kDa. The former allergen is assumed to be a member of group 10 allergens, whereas the later belong to haptenic allergens. Kata Kunci: Sarcoptes Scabiei, Allergens, Heat-Stable, Group 10, Hapten

Infection of Ornithobacterium rhinotracheale (ORT) in chickens in Indonesia Priadi, Adin; Natalia, Lily
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 1 (2006)
Publisher : Indonesian Animal Sciences Society

Ornithobacterium rhinotracheale is a bacterium identified as a new species in 1994 and generally associated with respiratory distress in chickens. From 214 of sinus swabs, tracheal swabs, lungs, airsac, liver heart blood samples and yolk sacs of chickens suffered from respiratory distresses, 6 isolates of O. rhinotracheale were isolated. These isolates were obtained from tracheal swabs of broiler chickens aged between 28-35 days old and broiler breeder of 32 weeks old. Upon incubated on blood agar for 48 hours at 37oC in a 5% CO2 atmosphere, round, convect and grey colonies with diametres of 1-2 mm were observed. The bacteria were pleomorphic, Gram negative rods, negative catalase and positive oxidase. Biochemically, the bacteria did not change potassium nitrate, tryptophan, glucose, arginine, urea, esculin, gelatine, arabinose, mannose, mannitol, N-acetyl-glucosamine, maltose, gluconate, caprate, adipate, malate, citrate and phenyl-acetate in API 20 NE system but Î² âgalactosidase was produced. In the API 20 NE system, the isolates were identified as 0020004, 0060004, 0020104 codes. Tracheitis, air sacculitis, pneumonia and cheesy air sacs were pathological changes generally found in chickens infected with O. rhinotracheale. Trachea is the most important organ for the isolation of O. rhinotracheale. Key Words: Ornithobacterium rhinotracheale, Infection, Chicken, Indonesia

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Home Page

OAI Link

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

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Abstract

Abstract

Abstract

Abstract

Abstract

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Abstract

Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA