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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

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Vaccination of goats with fresh extract from Sarcoptes scabiei confers partial protective immunity Tarigan, Simson
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Protective immunity has been known to develop in animals infested with Sarcoptes scabiei. However, our previous attempt to induce protective immunity in goats by vaccination with fractions of soluble or insoluble mite proteins had been unsuccessful. Degradation or denaturation of protective antigens occurred during vaccine preparation was suggested as one possible cause of the failure. In this study, mite proteins that used to immunise animals were prepared rapidly in order to prevent protein degradation or denaturation. About 150 mg of freshly isolated mites were rapidly homogenised, centrifuged then separated into supernatant and pellet fractions. Twenty-eight goats were allocated equally into 4 groups. Group-1 goats were vaccinated with the whole mite homogenate supernatant, group 2 with the supernatant, group 3 with the pellet, and group 4 with PBS (unvaccinated control). Vaccination was conducted three times, with three-week intervals between vaccinations, using Quil A as adjuvant, and each vaccination using fresh mite homogenates. One week after the last vaccination, all animals were challenged with approximately 2000 live mites. The severity of lesions, scored from 0 (no lesions) to 5 (>75% infested auricle affected), were determined one day, two days, then every week after challenge. Mite challenge caused the development of skin lesions in all animals. No significant differences between vaccinated and unvaccinated animals were observed in regards to the severity of lesions. However, the mite densities in vaccinated animals were significantly lower (P=0.015) than those in unvaccinated control. This study indicates that the protective antigens of S. scabiei are liable to degradation or denaturation and exist in a very low concentration or have vary low antigenicity. This implies isolation of the protective antigens by the conventional approach, fracionation of the whole mite proteins and testing each fractions in vaccination trials, is seemingly inappropriate for S. scabiei. Key Words: Sarcoptes scabiei, Vaccination, Fresh Homogenate, Partial Immunity

The activities of antioxidant enzymes extracted from Fasciola gigantica infecting Thin Tailed and Merino sheep Wiedosari, Ening
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Previous studies shown that Indonesian Thin Tailed (ITT) sheep are more resistant to Fasciola gigantica infection than Merino sheep. This difference could be mediated by intrinsic defense enzymes of the parasite. Certain enzymes are known to be crucial in parasite survival against host-derived immune responses. We measured some of them to identify if any comparative differences between the enzyme activities of the parasites from the two hosts (ITT & Merino sheep) could account for the mechanisms of parasite resistance to killing by the Merino host and susceptibility to killing by the ITT host. Parasites were extracted from the liver of infected ITT and Merino sheep and superoxide dismutase (SOD), glutathione S-transferase (GST) and catalase (CAT) enzyme activities were assayed. SOD and GST levels were found to be higher in parasites isolated from Merino than those of ITT sheep (P<0.05), CAT activity was not detected in any of the parasites. There was significantly higher eosinophils (P<0,05) in the ITT sheep peritoneal cells. These results suggested that SOD dan GST are important molecules in determining susceptibility in Fasciola-infected Merino sheep and resistance in Fasciola-infected ITT sheep. Key Words: Fasciola Gigantica, Indonesian Thin Tailed Sheep, Merino Sheep, Superoxide Dismutase (SOD), Glutathione S-Transferase (GST)

Phage typing and sensitivity test to antibiotics of Salmonella enteritidis isolates from Indonesia Poernomo, Sri; Priadi, Adin; Natalia, Lily
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Salmonella enteritidis (SE) is frequently implicated in disease outbreaks such as human food poisoning. Phage typing have been proved to be a valuable and sensitive tool in the control of SE infections. The ability of phage to distinguish varieties among apparently identical serotypes led to the development and acceptance of phage typing as a significant epidemiological procedure. To determine the epidemiological pattern of SE, phage typing of 53 SE isolated from various sources in Indonesia during 1991â1999, has been conducted using 16 typing phages of phage typing scheme of SE obtained from the International Collaborating Center for Enteric Phage typing, Central Public Health Laboratory, Colindale, UK. The lyse blood isosensitest was then used to test the sensitivity of the Salmonella isolates to antibiotics. The phage typing results obtained that of 53 Salmonella isolates there were one S. infantis, one S. berta, and 46 SE phage type 4, 2 SE phage type 7 (from chicken and water), 1 SE phage type 6 (from chicken) and 2 SE phage type 1 (from chicken). SE phage type 4 isolates comprised of 2 isolates from human, 19 isolates from chicken (young and adult), 17 isolates from day old chicks, 4 isolates from fluff, 2 isolates from chicken meat, 1 isolate from poultry farm water, 1 isolate from dog organ. These findings indicated that contaminated chicken appeared to be the sources of human and dog for SE infection. The results of sensitivity test of the isolates to antibiotics showed that most of the Salmonella isolates from Indonesia were resistant to the antibiotics tested. Key Words: Salmonella Enteritidis, Phage typing, Sensitivity test, Indonesia, Chicken

Nutritive values of solid heavy phase in replacing corn in broilers diet Sinurat, A.P; Purwadaria, T; Bintang, I.A.K; Pasaribu, T
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Indonesia is still importing corn for feed. Utilization of nonconventional feedstuffs may reduce this importation. One of them is solid heavy phase (SHP), i.e., a solid substance gained after filtration of liquid waste of crude palm oil factory with a potential production approximately 2 million ton/year. This research was carried out to study the utilization of SHP to replace corn in broilerâs diet. The SHP was dried and analysed for its nutrient compositions. The metabolisable energy was measured by using hens. Feeding trial was then conducted by replacing corn with SHP in various levels, i.e., 0 (Control), 10, 20, 30, and 40%. All diets were formulated to be isocalori dan isonitrogen. Five hundred broilers DOC were used in this trial and fed for 5 weeks. The performances of the birds were obsereved as parameters. Results showed that the ash, minerals crude fibre and fat contents of the SHP were higher than the corn. The crude protein of the Â SHP was similar to that of corn, but some amino acids content of the SHP were lower than the corn. The AME and TME values of SHP were 3271 and 3465 cal/g, respectively. Results of feeding trial showed that body weight of the birds were less as the level of SHP to replace corn increased in the diet. The FCR also showed impared as the levels of SHP increased in the diet. However, replacement of 10% corn with SHP did not significantly affect the body weight and FCR as compared with the control. Feed consumptions of the birds were not significantly affected by replacing corn with SHP. Carcass yield and relative weights of some internal organs were not significantly different between dietary treatments. It is concluded that the solid heavy phase could be used to replace 10% corn in broilerâs diet. Key Words: Solid Heavy Phase, Palm Oil By Products, Broilers

Effects of inclusion in ration sago with methionine and lysine supplementation on broiler performance and its abdominal fat content ., Suryana
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

An experiment has been conducted to study effects of sago inclusion in ration with methionine and lysine supplementation on broiler performance and its abdominal fat content. One hundred and eighty male broiler chicken of strain ISB-707 at the age of 7 days were randomly allocated to nine groups of experimental rations which were replicated four times (five bird each) in a completely randomized design. The treatments were; R0 (control), R1 (15.0% fresh sago), R2 (15.0% fresh sago + 0.1% methionine and 0.3% lysine), R3 (30.0% fresh sago), R4 (30.0% fresh sago + 0.1% methionine and 0.3% lysine), R5 (15.0% steamed sago), R6 (15.0% steamed sago + 0.1% methionine and 0.3% lysine), R7 (30.0% steamed sago) and R8 (30.0% steamed sago + 0.1% methionine and 0.3% lysine). The parameters recorded in this experiment were: feed intake, protein intake, energy intake, methionine intake, lysine intake, weight gain, carcass weight, carcass percentage and abdominal fat. Variables differences were examined for significances using Duncan test. Results showed that 15% steamed sago with supplementation of 0.1% methionine and 0.3% lysine significantly (P<0.05) increased the feed intake, weight gain, carcass percentage and carcass weight. Methionine and lysine supplementations decreased feed conversion ratio and abdominal fat content of broilers. Key Words: Fresh and Steamed Sago, Methionine, Lysine, Broilers

Utilization off fermented copra meal with A. niger in broiler diet Haryati, T; Togatorop, M.H; Sinurat, A.P; Purwadaria, T; ., Murtiyeni
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Research of nutritional evaluation of fermented copra meal and its application in broiler has been conducted. In the first experiment the crude protein and fiber contents were determined in the fermented copra meal using A. niger BPT, NRRL 337 and ES1. Further, 384 of Indian River DOC were used for in vivo experiment. Completed Randomized Design was used with 8 treatments: Control without and with 10% addition of copra meal, 3 levels of fermented copra meal addition with Aspergillus niger BPT or A. niger NRRL 337 (10, 15, 20%). Every treatment was repeated 8 time with 6 birds each. Variables measured were body weight, consumption, feed conversion, mortalities and carcass analyses. Diets were formulated in isocalori and isoprotein. The research was conducted in 6 weeks. The results showed that fermentation process increased the copra meal protein content from 22.3 to 36.1; 35.6 and 38.6% respectively for fermentation with A. niger BPT, NRRL 337 and ES1, decreased the crude fiber from 19.5 to 13.0; 18.9 and 8.28%. Results during 3 weeks trial show that the treatment significantly affected on broiler performance (P<0.05). Fermented copra meal was better than non fermented copra meal. There was no significantly different (P>0.05) in broiler performance at 6 weeks trial evaluation. The best conversion was in diet with 10% fermented copra meal with A. niger BPT and NRRL 337. The feed conversion of diets with 10% of each fermented products were better than control or diet without copra meal. The feed conversion of 15% fermented copra meal addition was not significantly different to control. Utilization of fermented copra meal with A. niger BPT or NRRL 337 should not more than 15%. Key Words: Copra Meal, Bioprocess, Broiler

Immunomodulatory effect of tea mistletoe (Scurrula oortiana) extract on chicken embryos Murtini, S; Murwanti, R; Satrija, F; Hadnharyani, E
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Tea mistletoe is one of medicinal herb which believed has an anticancer activity, itâs due to the capability of immunostimulator. The following research was carried out to determine the immunomodulatory effect of tea mistletoe (Scurrula oortiana) extract on chicken embryos. Twenty White Leghorn Specific Pathogen Free (SPF) 10 days old embryonated chicken eggs were divided into four groups of 5 eggs. The first group served as control and they were inoculated with aquabidestilate sterile. The second, third and fourth group was inoculated with 0.1 mg, 0.2 mg, and 0.4 mg S. oortiana extract/egg respectively. S. oortiana extract was inoculated via allantoic cavity. All experimental eggs were incubated at 37oC until day 21 and incubation was terminated before the embryos hatched. The embryos and the lymphoid organs (bursa of Fabricius, thymus and spleen) were weighed. Immunomodulatory effect of tea mistletoe extract was measured by counting the percentage of bursa of Fabricius active lymphoid follicle and the area of thymus medulla. The results showed tea mislestoe extract at the dose of 0.1mg, 0.2 mg and 0.4 mg have immunomodulatory effect on chicken embryos indicated by the increase of percentage of active lymphoid follicle of bursa Fabricius i.e. 68.8, 71.8 and 57.8% and increase area of thymus medulla i.e. 24.9 â 39.3% respectively compared to control group i.e. 22.6% of active lymphoid follicle of bursa Fabricius and 17.6% of thymus medulla area. It is concluded that S. oortiana extract at the dose of 0.1mg, 0.2 mg and 0.4 mg have immunomodulatory effect on chicken embryos. Key Words: Scurrula oortiana, Tea Mistletoe, Embryonated Chicken Egg, Immunomodulator

Dry matter productivity of the dwarf and normal elephantgrasses as affected by the planting density and cutting frequency Mukhtar, Muhammad
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

The effects of planting density and cutting frequency on dry matter productivity were compared in two years following establishment among dwarf varieties (early-heading, DE and late-heading, DL) and normal varieties, Wruk wona (Wr) and Merkeron (Me), in the southern part of Kyushu, Japan. The planting densities examined for Wr, DE and DL were high (16 plants/m2, 25 cm Ã 25 cm of spacing), medium (8 plants/m2, 50 Ã 25 cm), and low (4 plants/m2, 50 cm Ã 50 cm), while for Me was only medium. The cutting frequency was three times with 60-day intervals in 2002 and two times with 90-day intervals in 2003. Irrespective of the planting densities, dwarf varieties were higher in tiller number, leaf area index and percentage of leaf blade (PLB) than those of normal varieties, but lower in plant height and total dry matter weight at all planting densities in both years. With the increase in planting density, annual herbage dry matter yield (HDMY) increased. The annual HDMY was higher in 2002 (cut twice) than that in 2003 (cut three times), and the difference in annual HDMY between the dwarf and normal varieties decreased by planting at high density and cut twice. Even though, the dry matter productivity was higher in the normal varieties than in the dwarf varieties at any planting density and cutting frequency, DL tended to show a stable productivity with high PLB irrespective of planting density and cutting frequency. Key Words: Cutting Frequency, Dry Matter Productivity, Dwarf Elephantgrass, Normal Elephantgrass and Planting Density

Estimation of genetic distance of rabbit by morphometric analysis Brahmantiyo, B; Martojo, H; Monsjoer, S.S; Raharjo, Y.C
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

The observation on morphological body conformation of English Spot (ES), Flemish Giant (FG), New Zealand White (NZWm), and Rex (Rexm) from Magelang, Central Java, and New Zealand White (NZWb), Rex (Rexb), Satin (Satin) and RS (RS) from Balitnak-Ciawi, were carried out to determine estimation of Mahalanobis genetic distance.Â This research was held in Magelang (Central Java) and Balitnak-Ciawi (West Java), 237 heads of Rabbits were used. Eleven different body parts were measured, those were head (length and width), ear (length and width), chest (girth, depth, and width), humerus length, radius-ulna length, tibia length and body length. General Linear Models were used in this observation (SAS package program). Simple discriminant analyses as further analyses were done for head (length and width), chest (girth, depth, and width), humerus length, radius-ulna length, tibia length and body length. ES, FG and NZWm rabbits had morphological size bigger than others. Mahalanobis genetic distance showed that NZWm and NZWb, Rexm and Rexb were had differences with genetic distances of 5.89139 and 6.75571 respectively. Rabbits from Magelang and from Balitnak were different on morphometric with mahalanobis distance of that region ranges were 4.89426 to 6.96749. Results from canonical analysis showed that the most discriminant variables were obtained by chest girth, chest width and humerus length on first canonical and head length on second cannonical. Key Words: Rabbit, Genetic Distance, Morphometric

Status of ram spermatozoa DNA after freeze-drying process Saili, Takdir; Prasetyaningtyas, wahono Esthi; Setiadi, Mohamad Agus; AgungPriyono, Srihadi; Boediono, Arief
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

The process of freeze drying caused detrimental effect on plasma membrane and acrosome of the spermatozoa, even it potentially could alter the chromatin and DNA integrities. On the other hand, DNA integrity is essential for spermatozoa to participate in pronucleus formation during fertilization event. Therefore the evaluation of DNA integrity should be carried out to study the effect of freeze drying process. EDTA, EGTA, and PBS were used as dilution media of spermatozoa prior to freeze drying process to protect the DNA. Toluidine blue staining and comet assay methods were used to evaluate the alteration on chromatin and DNA integrities of spermatozoa, respectively. The results revealed that the highest compacted chromatin after 6 months storage of freeze-dried spermatozoa were observed from EGTA-3 (98%) and EGTA-1 (97%) treatments that had significant differences compared to all PBS treatments (90-92%), but not for fresh spermatozoa (100%). Whereas, the highest compacted DNA integrity of freeze-dried spermatozoa were observed from EGTA-2 (92%) and EGTA-3 (92%) but had no significant differences compared to other treatments including fresh spermatozoa (97%). These results demonstrate that EDTA and EGTA tend to be able to protect chromatin and DNA integrities of ram spermatozoa during freeze-drying and storage compared to PBS. Key Words: Freeze-Drying, Spermatozoa, DNA, Toluidine Blue, Comet Assay

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Home Page

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

Abstract

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Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA