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Contact Name
Ahmadi Riyanto
Contact Email
medpub@litbang.deptan.go.id
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Journal Mail Official
ahmadi_puslitbangnak@yahoo.com
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Kota bogor,
Jawa barat
INDONESIA
Jurnal Ilmu Ternak Veteriner
ISSN : 08537380     EISSN : 2252696X     DOI : -
Core Subject : Health,
Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary
Articles 1,756 Documents
The effect of of oligosaccharides from sweet potato on layers performance Haryati, Tuti; ., Supriyati
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.561 KB) | DOI: 10.14334/jitv.v16i3.613

Abstract

The investigation was carried out to study the utilization of oligosaccharides from sweet potato as feed additives to improve the performance of laying hens. Oligosaccharides were extracted from sweet potato using 80% ethanol. Extractant and residue were evaluated as feed additive. Ninety six of 18 weeks old laying hens from CP 909 Isa Brown were randomly distributed into four treatments with six replicates and four laying hens per replicate. The treatments were: Ro = Control without oligosaccharides, R1 = R0 + 0.1% of extract of sweet potato. R2 = R0 + with 0.2% of residue of sweet potato, and R3 = R0 +  with 0.2% of commercial FOS. The evaluations were carried out up to 25 weeks of egg production. The data were evaluated with statistical analysis using SAT. The results showed that egg production and FCR of R1 and R2 were better than R0 (P < 0.05). The treatments were not significantly affected feed consumption. In conclusion, addition of extract or residue of sweet potato improved (P < 0.05) egg production and FCR of layers compared to control diet. Key Words: Oligosaccharides, Sweet Potato, Performance, Layers
Genetic distance estimation of local swamp buffaloes through morphology analysis approach. Anggraeni, Anneke; Sumantri, C.; Praharani, L.; ., Dudi; Andreas, E.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.11 KB) | DOI: 10.14334/jitv.v16i3.614

Abstract

Information about genetic distances among populations (geographics) of local swamp buffalo is less published. Genetic distance analysis was done among seven local swamp buffalo populations trhough a morphological analysis. Female and male buffaloes were observed for a total number of 905 hds, originating from the Provices of NAD (100 hds), North Sumatera (51 hds), Banten (180 hds), Central Java (203 hds), South Kalimantan (121 hds), NTB (200 hds), dan South Sulawesi (50 hds).  Genetic variation among buffalo populations was calculated by discriminant function of Mahalonobis distance. Genetic distance was estimated by MEGA program. Morphometric measurements of female buffalo in North Sumatera were larger (P < 0,01) than those of buffaloes in Banten, South Kalimantan, NAD and South Sulawesi. Morphometric measurements of male buffalo in South Sulawesi were larger than those of buffaloes in Central Java, Banten, and NAD. Chest width was the highest different morphometric factor (0.969). Buffalo from NTB had the highest similarity (95.0%), followed by buffaloes from North Sumatera (74.51%) and South Sulawesi (74.19%); while the lowest one was from South Sulawesi (38.02%) and Banten (49.44%). Morphological distribution map indicated existence of three local swamp buffalo groups. Grup 1 was presented by buffalo from NTB, distributed in the II and III quadrants. Group 2 was presented by buffaloes from five locations (North Sumatera, Central Java, South Kalimantan, South Sulawesi, and Banten), distrributed in I and II quadrants, and a smaller part distributed in III and IV quadrants. Group 3 was presented by buffaloes from NAD and a smaller part from South Kalimantan and Banten, distributed in IV quadrant. The lowest genetic distance was identified between buffaloes in NAD and South Kalimantan (0.348), while the highest one was between buffloes in Banten and South Kalimantan (1.883). Based on phenogram tree structure, the observed local swamp buffaloes could be classified into 3 cluster, namely: Cluster 1 from South Kalimantan, NAD and Banten; Cluster 2 from South Sulawesi, Central Java and North Sumatera, and Cluster 3 from NTB. Key Words: Swamp Buffalo, Morphology, Discriminant Analysis, Genetic Distance
Performance of Garut breed rams fed diets containing various cation-anion difference with or without fish oil supplementation Hidayat, Rahmat; Toharmat, T.; Boediono, A.; Permana, I.G.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (64.371 KB) | DOI: 10.14334/jitv.v16i3.615

Abstract

This study was carried out to evaluate the effect of dietary cation-anion difference (DCAD) and fish oil supplementations on dry matter intake (DMI), dry matter digestibility (DMD) and organic matter digestibility (OMD), weight gain, as well as the acidity of urine in Garut breed rams. The experiment was done based on randomized group design with 6 treatments  and 3 groups. The dietary treatments were as follows: R0= basal ration (DCAD +14) without fish oil, R1 = basal ration (DCAD +14) with 3% fish oil, R2 = base ration (DCAD +40) without fish oil, R3 = base ration (DCAD +40) with 3% fish oil, R4 = acid ration (DCAD -40) without fish oil, and R5 = acid ration (DCAD -40) with 3% fish oil. All rations contained 150 ppm of zinc and were offered to 18 of Garut breed rams. The results indicated that DCAD +40 and -40 decreased DMI significantly.  The ration with DCAD +40 had the lowest DMI. Fish oil supplementation decreased DMI. No differences were observed for DMD and OMD.  DCAD +40 and -40 decreased body weight of rams. However, the body weight of rams was very low. Variation of urine pH followed the DCAD pattern. It was concluded that DCAD +40 and -40, as well as fish oil supplementations decreased DMI, body weight and urine pH followed the DCAD pattern. Keys Words: Cation, Anion, Body Weight, Digestibility, Garut Breed Rams
Rate of temperature reduction at cryopreservation primordial germ cells (PGC) of three Indonesian native chicken. Kostaman, Tatan; Sopiyana, S.; Setioko, A.R.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.286 KB) | DOI: 10.14334/jitv.v16i3.616

Abstract

Primordial germ cells (PGCs) are original cells of spermatogonia in the testes or oogonia in the ovary. PGCs in poultry can be harvested and stored in the liquid nitrogen and can be used for conservation as genetic materials of poultry. The objective of this study was to obtain the optimal rate of temperature reduction on PGCs quality from three different Indonesian native chicken after thawing. Fertile eggs obtained from native chicken were incubated for 56 hours to obtain embryo at stage of 14-16. PGCs were isolated from the blood using modified Nycodenz Gradient Centrifugation technique. There after they were kept in a straw and equilibrated for 15 minutes at 5oC and frozen at the rate of temperature reduction of 0.3, 0.5, and 1oC per minute using embryo freezing machine (FHK Fujihara: ET-1). After the temperature reached -30oC, then they were plunged directly into the liquid nitrogen. Recovery rate and viability of PGCs after freezing and thawing were measured. The results of this study showed that the average recovery rate of PGCs that have been frozen at rate of temperature reduction of 1, 0.5, and 0.3oC per minute were 35.6, 43.9, and 44.9% respectively. However the rate of temperature reduction of 0.5 and 0.3oC per minute did not significantly affect the recovery rate. The average percentage of viability of PGCs that were frozen at the rate of 1, 0.5 and 0.3oC per minute were respectively 62.6, 77.5, and 77.4%. It seems that the viability followed the trend of recovery rates where the 1oC per minute reduction was the lowest quality compared to the other two treatments. It is concluded that the reduction of 0.5 or 0.3oC per minute are considered as the ideal temperature reduction when native chicken PGCs are frozen. Key Words: PGCs, Native Chicken, Conservation
Comparison of serological test for toxoplasmosis using Immunostick Assay, ELISA and Latex Agglutination Test Subekti, Didik T.; Kusumaningtyas, E.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.755 KB) | DOI: 10.14334/jitv.v16i3.617

Abstract

Many serological methods were developed in order to improve detection and identification of an infectious diseases. ELISA (enzyme linked immunosorbent assay), is a well-known as the most serological technique and widely used for infectious disease diagnoses, especialy toxoplasmosis in human and animal. Unfortunately, the technique requires specific tools and expertise that are not always available in the field. Inn addition, ELISA needs longer process than other techniques for both small and large number of samples. Another serological assay, namely Latex Agglutination Test (LAT) was known as populer and mostly applied in field for toxoplasmosis diagnosis in Indonesia, both for human and animal serum. However, the accuracy of this technique was slightly lower compared to ELISA, especially for weakly seropositive. Recently, an immunostick assay technique was developed as alternative serological method which has high accuracy as ELISA. The immunostick assay was rapid and simple like latex agglutination test. The immunostick assay was evaluated to detect Toxoplasma gondii infection in goat serum samples and compared to ELISA and LAT. The immunostick assay had a true agreement 95.88% - 100% againts ELISA and their strength of agreement was very good aggreement with ELISA (k = 0.796; AC1 = 0.948). However, LAT had true agreement only 75.74%, either with ELISA or immunostick assay. The strength of agreement of LAT was moderately aggreement with ELISA or immunostick assay (k = 0.447; AC1 = 0.582). Therefore, the immunostick assay could become the first choice for rapid serological assay on toxoplasmosis diagnosis, both in the laboratory and in the field. Key Words: Latex Agglutination, ELISA, Immunostick Assay, FELISA, Toxoplasmosis
In vitro expression of native H5 and N1 genes of avian influenza virus by using Green Fluorescent Protein as reporter Hartawan, Risza; Robinson, K.; Mahony, T.; Meer, J.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1261.919 KB) | DOI: 10.14334/jitv.v16i3.618

Abstract

The hemagglutinin and neuraminidase are important immunogen of avian influenza virus that are suitable for recombinant experimentation. However, both genes have been experienced rapid mutation resulting in diverse variety of genotypes. Hence, gene expression in recombinant systems will be difficult to predict. The objective of the study was to examine expression level of H5 and N1 genes from a field isolate by cloning the genes into expression vector pEGFP-C1. Two clones respresenting fulllength of H5 and N1 gene in plasmid pEGFP-C1 were transfected into chicken embryo fibroblasts (CEF), rabbit kidney (RK13) and African green monkey kidney (VERO) cells using Lipofectamine ‘Plus’ reagent. The experiment showed level of gene expression in the VERO cell was higher than in the RK13 and CEF cells. Observations using fluorescent microscopy and Western blotting revealed that the N1 gene was expressed better in all cells compared to the H5 gene. Key Words: H5N1 Virus, Hemagglutinin, Neuraminidase, Gene Expression, Green Fluorescent Protein
Improving productivity of Rex, Satin and Reza rabbits through selection Brahmantiyo, Bram; Raharjo, Y.C.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 4 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.099 KB) | DOI: 10.14334/jitv.v16i4.619

Abstract

Selection based on weaning weight in Rex, Satin rabbits and their crosses was done to improve its productivity. Data from the base population (P0), selected population (G0) and an offspring of selected population (F1) were used to estimate heritability using nested analysis method (nested) and best linear unbiased prediction (BLUP). The value of heritability estimated of birth weight, weaning weight, 12 weeks body weight and 16 weeks body weight of Rex were 0.74±0.09, 0.93±0.05, 0.81±0.09 and 0.89±0.06, Satin were 0.96, 0.82±0.22, 0.93±0.40 and 0.97 and Reza were 0.96±0.27, 0.98, 0.86±0.40 dan 0.78, respectively. Increase in weaning weight on selected Rex, Satin rabbits and their corsses were 22.77 g (3.66%), 6.83 g (1.11%) and 65.29 g (10.67%). Key Words: Selection, Rabbit, Heritability, Weaning Weight
Polymorphism of growth hormone receptor (GHR) gene in Holstein Friesian dairy cattle Misrianti, Restu; Sumantri, C.; Anggraeni, A.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 4 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.675 KB) | DOI: 10.14334/jitv.v16i4.620

Abstract

Growth hormone gene have a critical role in the regulation of lactation, mammary gland development and growth process through its interaction with a specific receptor. Growth hormone (GH) is an anabolic hormone which is synthesized and secreted by somatotrop cell in pituitary anterior lobe, and interacts with a specific receptor on the surface of the target cells. Growth hormone receptor (GHR) has been suggested as candidate gene for traits related to milk production in Bovidae. The purpose of this study was to identify genetic polymorphism of the Growth Hormone Receptor (GHR) genes in Holstein Friesian (HF) cattle. Total of 353 blood samples were collected from five populations belonging to Cikole Dairy Cattle Breeding Station (BPPT-SP Cikole) (88 samples), Pasir Kemis (95 samples), Cilumber (98 samples), Cipelang Livestock Embryo Center (BET Cipelang) (40 samples), Singosari National Artificial Insemination Centre (BBIB Singosari) (32 samples) and 17 frozen semen samples from Lembang Artificial Insemination Center (BIB Lembang). Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques then PCR products were genotyped by the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods. There were two allele dan three genotypes were found namely: allele A and G, Genotype AA, AG and GG repectively. Allele A frequency (0.70-0.82) relatively higher than allele G frequency (0.18-0.30). Chi square test show that on group of BET Cipelang, BIB Lembang and BBIB Singosari population were not significantly different (0.00-0.93), while on group of BET Cipelang, BIB Lembang dan BBIB Singosari population were significantly different (6.02-11.13). Degree of observed heterozygosity (Ho) ranged from 0.13-0.42 and expected heterozygosity (He) ranged from 0.29-0.42. Key Words: Growth Hormone Receptor, Polymorphism, Holstein Friesian Cattle
The growth of Java bulls fed rice straw and concentrates containing different levels of protein Adiwinarti, Retno; Fariha, U.R.; Lestari, C.M.S.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 4 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.273 KB) | DOI: 10.14334/jitv.v16i4.621

Abstract

This study was performed to determine the growth of Java bulls fed rice straw and concentrates at different levels of protein. Twelve heads of Java bulls, one and a half years old, with initial body weight ranging from 113.83-191 kg were used in this experiment. They were grouped into four replications based on the initial body weight. The rice straw (30%) and concentrates (70%) diet containing three different levels of protein (8.27; 11.03 and 14.43%) were fed during nine weeks. Data gathered were the average daily gain and the average body measurements (chest girth, shoulder height and body length). Result of this study showed that the increase of protein levels from 8.27 to 14,43% did not significantly influence the average daily gain, shoulder height, and body length of Java bulls (P > 0.05), but it influenced daily chest girth (P < 0.05). The average daily gain (ADG), shoulder height and body length were 0.633 kg, 0.08 cm, and 0.09 cm, respectively. The highest average of daily chest girth of Java bulls was T2 = 0.19 cm, followed by T3 = 0.15 cm, and T1 = 0.12 cm. It is concluded that the increase levels of protein from 8.27 to 14.43% did not affect the average daily gain, shoulder height and body length of Java cattle. However, the highest chest girth was achieved by Java cattle which fed concentrate containing 11.03% of protein. Key Words: Growth, Java Bull, Rice Straw, Concentrate, Levels of Protein
Effect of fermented noni leaf (Morinda citrifolia L.) in diets on cholesterol content of broiler chicken carcass Syahruddin, Erman; Abbas, H.; Purwati, E.; Heryandi, Y.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 4 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (88.957 KB) | DOI: 10.14334/jitv.v16i4.622

Abstract

Chicken meat is very nutritious. It is sometimes blamed to cause strock attack and coronary heart disease in human, because of high fat and cholesterol contents in the chicken meat. Therefore, the aim of this experiment is to evaluate the effect of fermented noni leaf levels in diets on the cholesterol content of broiler chicken carcass. The experiment was based on completely randomized design with eight experimental diets containing 0, 3, 6, 9, 12, 15, 18 and 21% of fermented noni leaf powder. All diets were formulated to contain 22% crude protein and 3000 kcal/kg. Each treatment had three replicates with ten chickens per replicate. Two hundred and forty day old unsex broiler chicks Arbor Acress were fed ad lib. for eight weeks and then sacrificed. Feed consumption, body weight gain, feed conversion ratio, and cholesterol content of carcass were taken as variable responses. Data were analyzed based on analysis of variance and orthogonal comparisons. Results showed that feed consumption, daily weight gain, FCR and carcass content were not affected by the levels of fermented noni leaf in the diet. However, cholesterol content of broiler carcass was significantly (P < 0.05) affected by the dietary treatments. Cholesterol content of the carcass was reduced processed 26.18% 73.06 to 53.76 mg/100g mg/100g chicken meat. The lowest cholesterol level was obtained by feeding the chickens with diets containing 21% fermented noni leaf. Key Words: Morinda citrifolia L., Cholesterol, Broiler Chickens

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