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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

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Variation on DNA microsatellite of Holstein-Friesian dairy cows in Baturaden Dairy Cattle Breeding Center Sumantri, C; Anggraeni, A; Farajallah, A; Perwitasari, D
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Eight microsatellite loci of which the locations were three on chromosome 6 (BTA6) namely CSN 3, BM 143 and BM 415; two on chromosome 9 (BTA9) namely ETH 225 and BM 4208; and three on chromosome 10 (BTA10) namely BP 31, BM 1237 and BM 888 were used to investigate genotypic variation of Holstein-Friesian (HF) dairy cows in Baturraden Dairy Cattle Breeding Centre. Research activities were carried out through some steps involving blood collection, DNA isolation, amplification on DNA fragments by polymerase chain reaction (PCR) and separation by electrophoresis with silver staining. Frequency and heterozygosity of genes under consideration were calculated. The results showed that the eight microsatellite loci exhibited a total number of 33 alleles. Locations of those alleles were five in BM 143 and BM 4208 loci; four in BM 415, CSN 3, ETH 225, BM 1237, BM 888 loci; and three in BP 31 locus. A number of 14 out of 33 alleles might be specific alleles for HF dairy cattle in Baturraden Dairy Cattle Breeding Centre. The lowest heterozygosity per locus (Ä¥) was 0.6151 for BM 415 whilst the highest one was 0.7301 for BM 888. Additionally, the average heterozygosity for all loci (Ä¤) detected in this study was 0.6768. The genotype BB on locus BM 143 and AC on locus CSN-3 significantly affected (P<0.05) the estimated breeding value of milk yield of HF cows in this location. Key Words: Holstein-Friesian Cows, DNA Microsatellite, Allele, EBV of Milk Yield

Quality of canine epididymal spermatozoa during storage at 4ï°C Setiadi, M.A; ., Yulnawati; Suprayogi, A
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

The aim of this present study was to investigate the quality of canine epididymal spermatozoa during storage at 4Â°C. Spermatozoa was collected by flushing technique with physiological saline (NaCl 0.9% w/v) and diluted in modified Tris based extender containing 20% (v/v) of egg yolk for three days. The result showed that mean concentration of spermatozoa from cauda epididymal was 95.29.106 spz/ml. The percentage of progressive motility and membrane integrity of spermatozoa on time of collection was 70.71% and 72.85%, respectively. Quality of epididymal spermatozoa was decreased significantly (P<0,05) during storage at 4Â°C. The percentage of progressive motility during storage were 70.71% on day 0 (H-0, after diluted), 60.71% (H-1), 45.71% (H-2), and 33.57% (H-3). The percentage of membrane integrity during storage were 72.85, 68.88, 61.06 and 47.47% on H-0, H-1, H-2 and H-3, respectivelly. In conclusion, quality of canine epididymal spermatozoa was decreased during three days of storage at 4Â°C. Key Words: Epididymal Sperm, Storage, Canine

Stallion semen cryopreservation using different cryoprotective agents on the skim milk trehalosa extender Arifiantini, R.I; Supriatna, I
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Cryoprotective agents (CPAs), protect the sperm during cryopreservation. There are two general classes of CPAs, at first penetrating cryoprotectants, these pass through the sperm membrane and act both intracellular and extracellularly, and the second non-penetrating cryoprotectants, these act only extracellularly. The objective of this study was to evaluate the addition of different CPAs namely glycerol (G), combination of ethylene glycol with glycerol (EG) and dimethilformamide (DMF) using skim milk trehalosa extender. The semen collected from 3 stallions using artificial vagina twice aweeks. The semen was evaluated, centrifugated and diluted in skim milk extender supplemented with 50 mM trehalose and three different CPAs with the concentration of sperm were 200x106 ml-1. Extended semen was then packed into minitub 0.3 ml and equilibrated at 4oC for 2 hours, freeze in the liquid nitrogen vapor for 10 minutes and stored in liquid nitrogen container -196oC. After 24 hours, the semen was thawed at 37oC for 30 second. Results of this experiment indicated that the percentage of motile and viable sperm in skim trehalosa extender using DMF and glycerol better than combination of ethylene glycol and glycerol. Â Key Words: Cryopreservation, Stallion Sperm, Cryoprotectant

Rescuing genetic material of unexpectedly die animal Said, Syahruddin; Saili, Takdir
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Rat cauda epididymides were kept in 1.5-ml centrifuge tubes containing 1 ml milli-Q water or physiological saline (0.9% NaCl) and stored and freezed at -196ÂºC without cryoprotectant for up to 7 days. After thawing of the cauda epididymis, all spermatozoa were non-motile immediately after collection. All oocytes injected with sperm heads (nuclei) of spermatozoa collected from frozen-thawed cauda epididymis in saline were activated (100%) and gradually decreased (P<0.05) in cauda epididymis frozen in milli-Q water at -196Â°C (86%), and in control (69%). In activated oocytes, a large proportion of sperm heads had transformed into male pronuclear formation (66-78%). When 1-cell embryos were cultured for 120 h, 7% developed to blastocyst stages. These results indicate that genetic materials of species (at least in the rat) that had unexpectedly die can be saved by a simple method. Key words: ICSI, Sperm Heads, Piezo-Injection, Frozen, Rat

Characterisation of enzymatic activities of H5N1 influenza virus Tarigan, Simson; Indriani, Risa; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

One of the two glycoproteins projected from the surface of the influenza virus is identified as neuraminidase. This enzyme enables the virus to spread in the host, and therefore it plays vital roles in the viral pathogenicity. From the viewpoint of disease control, neuraminidase is used as the target for the development of anti-flu drugs, and for the development of diagnostic test to differentiate infected from vaccinated animals (DIVA). Since the roles of the enzyme are very important, information regarding the characteristics and the procedure to measure its activity, which is the purpose of this study, is essential. The optimum incubation time of the neuraminidase-substrate (fetuin) reaction and the optimum pH of the buffer were determined. The stability of the enzyme against heating, supplementation or chelating of calcium ion, and b-propiolactone treatment were analysed. This study showed that neuraminidase from H5N1-influenza virus was, in regards to the characteristics investigated in this study, was comparable to that from Clostridium perfringens. The optimum incubation time for the viral and Clostridial neuraminidases were 60 and 30 minutes, respectively; whereas, the optimum pH for both neuraminidase was 6-7. At pH 8, both neuraminidase were inactive. Supplementation of calcium ion tended to increase activity but chelating of the cation did not have any observable effects. Treatment with 0.2% b-propiolactone for 6 hours reduced the activity, whereas heating at 60Â°C for 60 minutes abolished all activity. Since inactivation by b-propiolactone is partially only, neuraminidase assay could be performed safely in ordinary laboratories using b-propiolactone-treated-influenza virus, rather than the life virus. The thermolabile nature of the enzyme will complicate any attempt to purify the enzyme. Key Words: H5N1, Neuraminidase, Stability, Thiobarbituric Assay

Waterfowl potential as resevoirs of high pathogenic avian influenza H5N1 viruses Susanti, R; Soejoedono, R.D; Mahardika, I-G.N.K; Wibawan, I-W.T; Suhartono, M.T
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

The high population of waterfowl subsequently with the high case fatality of poultry and people in West Java regency caused by HPAI H5N1 can raise possibility that waterfowl was a natural reservoir. This research aimed to prove that waterfowl in West Java served as reservoir of AI virus (primarily H5N1) and also identify the virus pathotype based on cleavage site of amino acid sequence. Cloacal swab sample was obtained from healthy and unvaccinated waterfowl from Sukabumi and Bogor Regency. Cloacal swab was propagated in 9 days old embryonic chicken eggs. Allantoic fluid was harvested at the 4th day of incubation and then tested for hemagglutination, and positive isolate continued with virus sub-typing using PCR method. H5 gene from H5N1 isolate then sequenced using dideoxy termination method. Multiple alignment of nucleotide sequences were analysed using MEGA-3.1 program. Sub-typing using PCR method indicated the existence of 25 strain H5N1, 16 strain HxN1, 4 strain H5Nx and 9 virus ND. Characterization of cleavage site amino acid sequence indicated that all H5N1 sample were pathogenic with sequence QRERRRKKR (23 sample) dan QRESRRKKR (2 sample). Waterfowl was HPAI H5N1 virus reservoir. Asymptomatic infection in waterfowl, but the virus shedding gradually occurred and therefore it became potential source of H5N1 virus infection. Our findings suggest that immediate action is needed to prevent the transmission of highly pathogenic avian influenza viruses from the apparently healthy waterfowl into terrestrial poultry or human. Key Words: HPAI, H5N1, Reservoir, Water Fowl

Supplementation of Morinda citrifolia waste as bioactive compound on the performances of broiler Bintang, I.A.K; Sinurat, A.P; Purwadaria, T
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

A study on the use of dried Morinda citrifolia waste as feed additive in broiler ration was conducted. One hundred day old chicks (doc) were allocated into 4 levels (control; 0.0; 1.2; 2.4 and 4.8 g/kg feed) of M. citrifolia waste with 5 replication. Each replication had 5 birds. The treatments were allocated in a completely randomized design. Variables measured were: feed intake, live weight, feed conversion ratio (FCR), percentages of carcass and internal organs ( liver. gizard, abdominal fat, and thickness of intestine). The results showed that M citrifolia waste supply did not significantly (P>0.05) affect all variables measured, but feed intake of those fed with high levels (4,8 g/kg) of M. citrifolia waste was significantly (P<0.05) lower than those fed with 1.2 g/kg. FCR was significantly (P<0.05 ) lower as compared to the control; 1.2 and 2.4 g/kg. It is concluded that the best treatment was the supplementation of M. citrifolia waste of 4.8 g/kg, this treatment improved feed efficiency by 5% as compared to the control. Key Words: M. citrifolia Waste, Performances, Broiler

Effect of humid acid on performance of broiler chicken Kompiang, I.P; ., Supriyati
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

An experiment had been carried out to study the effect of supplementation of humic acid via drinking water as natural feed additive on the performance of broiler chickens, in order to find the replacement of antibiotic, which recently known had negative effect on the consumen. Three hundred and twenty of day old broilers were divided into 4 treatment groups: (I) control (without humic acid); (II), (III) and (IV) with 100, 200 and 300 mg l-1 humic acid in the drinking water, respectively. Each group consisted of 4 replications with 20 birds each replication. The drinking water contained 2 mg l-1 probiotic. The birds were kept in litter system; feed and water were given ad libitum for 5 weeks. The parameter measured were body weight gain, feed convertion ratio (FCR) and percentage of carcass. The treatments had a significant effect on both body weight gain (P<0.05) and FCR (P<0.05). Body weight gain of control (I) 1839 g head-1 5 weeks-1, with FCR 1.66 were poorer than that of the group received humic acid: 1882 g head-1 5 weeks-1, 1.66; 1881 g head-1 5 weeks-1, 1.64; and 1874 g head-1 5 weeks-1, 1.59 for treatments II, III and IV, respectively. There were no significant different (P>0.05) between treatment II, III and IV neither on body weight gain nor FCR values.The treatments had no significant effect on carcass yield, 66.28 Â± 1.78; 67.06 Â± 3.11; 67.63 Â± 2.06 and 67.68 Â± 2.21% for treatments I, II, III and IV, respectively. It is concluded that humic acid has a potential effect as natural feed additive, which could improve growth and feed efficiency without affecting carcass yield. Key Words: Humic Acid, Natural Feed Additive, Broiler

Effect of restricted feeding on productivity of Mojosari X Alabio cross-bred layer ducks (MA): Growing phase to point at lay Ketaren, P.P; Prasetyo, L.H
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

Feed efficiency of layer ducks is low since feed consumption is considerably higher than egg production. The experiment has been conducted to evaluate performances and age at first laying of MA crossbred duck from 1-20 weeks old on restricted feeding. Three experimental diets tested were: (1) 70% ad libitum = 70% A, (2) 85% ad libitum = 85% A and (3) 100% ad libitum = 100% A. Feed consumption, live weight gain (PBB), feed conversion ratio (FCR), mortality rate and age at first laying were recorded. Live weight gain of ducks on 70 and 85%A were significantly (P<0.05) lower than 100% A for both at 12 weeks old. Live weight gain of duck at 16 weeks old fed 85% A was similar to the ducks fed 100% A. On the other hand, FCR of duck at 16 weeks old on 70 and 85% A were significantly (P<0.05) better than ducks fed 100% A diet. Age at first laying was delayed 9 days on duck fed 85% A and 21 days on duck fed 70% A diet. However, average weight of first laid egg was not affected by the treatments. Mortality increased on duck fed 70% A. This study indicated that feeding MA duck at rate of 85% of ad libitum was sufficient to support normal growth and reproductive development. Key Words: MA Crossbred Duck, Restricted Feeding, Growth, Age at First Laying

Palm oil by-product as an alternative feedstuff in Central Kalimantan: 2. The effect of feeding palm oil mill effluent on cholesterol, fatty acid and vitamin A of broilers Widjaja, Ermin; Utomo, Bambang Ngaji
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

Solid heavy paste, a byproduct of crude palm oil processing, is found in an excessive amount in Central Kalimantan. It is estimated that 400 tons of this material were produced per day by crude palm oil (CPO) factories in this region. This material contains 12.63-17.41% crude protein, 9.98-25.79% crude fiber, 7.12-15.15% crude fat, 3217-3454 kcal/kg (gross energy) and 1.5% CPO. Considering its composition, this material can be used as an ingridient in broilerâs diet. This study was conducted on broiler chickens of Hubbard strain in Kapuas district. The objectives of the experiment were to study the cholesterol, fatty acid and vitamin A retained in meat and liver of broilers as a result of feeding ration containing solid heavy paste. This experiment was arranged in a completely randomized design. The treatments were levels of solid in the diet, i.e., 0.0, 12.5, 25 and 37.5%. Four hundred broilers were divided into 4 treatmen groups with 5 replications, consisted of 20 chickens in each replication. Data was analyzed based on statistical and descriptive analyses. The results of this experiment indicated that 25% solid in the diet decreased the total fat and cholesterol in the meat and liver, but increased polyunsaturated fatty acid contained in meat. The highest contain of vitamin A in meat and liver was found in the group that received diet containing 12.5% solid. Key Words: Solid, Broiler Chickens, Cholesterol, Fatty Acid, Vitamin A

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Home Page

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

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Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA