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Edy Kurnianto
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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 18 Documents
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Search results for , issue "Vol 43, No 4 (2018): December" : 18 Documents clear
Measurement of several qualitative traits and body size of Lombok Muscovy Ducks (Cairina moshcata) in semi-intensive rearing Tamzil, M.H.; Lestari, L.; Indarsih, B.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.333-342

Abstract

The study was conducted to identify phenotypic characteristics of Lombok Muscovy ducks. A total of 100 adult male and 100 adult female Muscovy ducks. The research location was determined by purposive random sampling, while the determination of farmers and Muscovy ducks was done incidentally. Observations were made on each Muscovy duck by observing the color, body weight and size of body parts. The body size data were tabulated, averaged, and then analyzed descriptively; meanwhile, the plumage color data were calculated in frequency. To find the relation between size of body parts and body weight, data were analyzed using simple regression. The study found that the male Muscovy ducks had two plumage characteristics, consisting of white and black-white, both shared the same frequencies, while in the female Muscovy ducks, white color was more dominant compared to the black-white color. The colors of the beak were divided into two i.e., black with a white color in the middle and reddish-white with pink colors in the middle. The size of Lombok Muscovy ducks was rather small in comparison with the other Muscovy ducks. The size of male duck was larger than that of the female.
Estrus and pregnancy rate of Simmental-Ongole Crossbred and Ongole Grade heifer after being synchronized and inseminated Sutiyono, S.; Samsudewa, D.; Suryawijaya, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.438-444

Abstract

The aim of the study was to examine the synchronization of estrus and artificial insemination in heifers of Ongole grade and Simental-Ongole grade. The research used 19 Ongole Grade (OG) and 18 Simental-Ongole Crossbred (SOC) heifers. This research applied purposive sampling to determine the research population. The criteria to select the population were heifers which have in mature body. The normality of heifer’s reproductive organs were identified by performing rectal palpation, then 50 mg of medroxy progesterone acetate on vaginal sponge was used to synchronized estrus. The estrus heifer was inseminated using frozen semen. The observed parameters were the number of heifers with normal and abnormal reproductive organs, estrus sign and pregnancy. Data were analyzed descriptively. This study showed that OG and SOC with abnormal reproductive organs was 1 heifer (5.56%) and 7 heifers (36.84%), respectively. The responses of estrus synchronization in heifers with normal and abnormal reproductive organs were 94.17% and 100% in OG, and 100% and 42.86% in SOC. Meanwhile, the pregnancy rate was 70.59 and 0% in OG, and 50.00 and 0% in SOC. In conclusion, the number of SOC heifers with abnormal reproductive organs is higher than OG. Estrus synchronization using 50 mg medroxy progesterone acetate increase the amount of estrus and pregnancy in heifers with normal reproductive organs.
Blood parameters and productivity of broilers fed ration composed of microparticle protein with the addition of Lactobacillus sp. Wulandari, L.T.; Suthama, N.; Sukamto, B.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.396-404

Abstract

The study was aimed to evaluate the effectiveness of feeding dietary microparticles protein added with Lactobacillus sp. on blood parameters and broiler productivity. A total of 192 birds of 21 days old broiler with initial body weight of 481 ± 67 g were divided into 8 treatments and 4 replications (6 birds each) of a completely randomized design (CRD). Dietary treatments were T0: 21% intact protein ration, T1: 18% intact protein ration, T2: 21% microparticle protein ration, T3: 18% microparticle protein ration, T4: T0 + 1.2 mL Lactobacillus sp, T5: T1 + 1.2 mL Lactobacillus sp., T6: T2 + 1.2 mL Lactobacillus sp., and T7: T3 + 1.2 mL Lactobacillus sp. Parameters observed were total lactic acid bacteria and Coliform, pH, blood cholesterol, low density lipoprotein (LDL), high density lipoprotein (HDL), meat cholesterol, meat weight, feed consumption, and body weight gain (BWG). Data were analyzed using analysis of variance and followed by Duncan test (P<0.05). The results showed that T7 treatment significantly (P<0.05) decreased blood cholesterol, LDL, and meat cholesterol, but increased HDL, meat weight, and BWG. Blood cholesterol, LDL, and meat cholesterol were indicated significantly (P<0.05) lowest values due to T7 treatment. In conclusion, feeding 18% microparticle protein with addition of 1.2 mL Lactobacillus sp. can improves blood status and increases broiler productivity.
Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat Latifah, L.; Maharani, D.; Kustantinah, A.; Hartatik, T.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.343-351

Abstract

Melanocortin 4 receptor gene is a potential marker gene for growth performance in ruminant and non-ruminant animals. The objective of this study was to perform allele and genotype frequencies, and association of melanocortin 4 receptor with growth traits in Bligon goat. Seventy seven blood samples were collected and DNA isolated successfully. One pair primers were used for PCR of MC4R gene (F: 5’-TCGGGCGTCTTGTTCATCAT-3’ and R: 5’-CAAGACTGGGCACTGCTTCA-3’). Two SNPs (g.998A/G and g.1079C/T) were identified in exon of Bligon MC4R by DNA sequencing and PCR-RFLP. The SNP g.998A/G identified amino acid change from isoleucine to methionine (I-M), while the SNP g.1079C/T was synonymous polymorphism. Based on relationship analysis between Genotype of MC4R gene (SNP g.998A/G) and growth traits indicated that GG genotype was lowest in weaning weight (WW; AA=13.0±1.7; AG=11.2±3.6; GG=9.2±3.9), weaning body length (WBL; AA=46.8±2.4; AG=46.3±6.0; GG=43.8±5.3) and weaning chest circumference (WCC; AA=50.9±4.0; AG=50.7±4.6; GG=47.1±7.3) compare to AA and AG genotype (p<0.05). Individual with CC and CT genotypes at SNP g.1079C/T had higher (P<0.05) in WW (CC=12.6±2.1; CT=12.5±2.5) and average daily gain (ADG; CC=81.2±21.8; CT=79.3±26.2) than TT genotype (WW=10.6±3.1; ADG=58.3±30.5). This results suggested that SNP g.998A/G and g.1079C/T of MC4R gene could be used as the genetic marker of growth traits for selection in Bligon goat.
Effect of Lactobacillus sp. probiotics on intestinal histology, Escherichia coli in excreta and broiler performance Hidayat, M.N.; Malaka, R.; Agustina, L.; Pakiding, W.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.445-452

Abstract

The study was conducted to determine the effect of Lactobacillus sp. in intestinal histology, total amount of Escherichia coli in broiler chicken excreta and broiler performance. Research has been conducted using a completely randomized design with 4 treatments and 5 replications utilize 15 chickens. Lactobacillus sp. 5.8 x 107 CFU/mL was mixed in drinking water and also feed commercial concentrates together ad libitum. The results showed that treatment had significant effect (P <0.05) on weight gain, ration conversion, and final weight. There was no difference (P>0.05) ration consumption on doses of Lactobacillus sp. treatment 0 mL/days, 1 ml/days, 3 ml/days, and 5 ml/days. In general, the probiotic treatments of 1, 3, and 5 mL/days of lactobacillus sp. were better at villus height, villus surface area, weight gain, final weight, and feed conversion compared to control. Treatment a dose of 3 mL / d (1.7 x 108 CFU) is the best dose in providing optimal response to histology of the small intestine, and broiler performance. Treatment 5 mL/days has the lowest amount of Escherichia coli in manure of broiler. In conclusion, Lactobacillus sp. can be used as one candidate probiotic for broiler.
Effect of two pre-freezing methods on quality of sexed semen in Ettawa Grade goat Ondho, Y.S.; Udrayana, S.B.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.405-411

Abstract

The aim of this study was to determine the influence of pre-freezing different procedures to the quality of Ettawa Grade goat frozen semen-sexing. The research material was semen sexing consisted of 2 layers those were top layer and bottom layer. The quality of frozen semen sexing was observed by comparing the pre-freezing technique (factory standard operating procedure: FSOP) according to the  Artificial Insemination Center operating procedure (pre-freezing by placing the straw of semen for about 4 cm above liquid nitrogen for 9 minutes) with the modification procedure (MP), pre-freezing by placing the straw of semen 16 cm above liquid nitrogen for 9 minutes and then it was lowered to 4 cm above liquid nitrogen for 9 minutes during the pre-freezing phase. The parameters observed were motility, progressive motility, hyperactivation, and sperm linearity. Data were analyzed using Student's t-test.The results of this study indicated that the quality of sexed-semen in the standard operating procedures of frozen semen compared to the treatment of modifications to the top and bottom layers were motility at the top layer (46.06 ± 7.52% vs 55.6 ± 7.78%) and bottom layer (36.82 ± 6.49% vs. 41.47 ± 6.57%); progressive top layer (16.34 ± 4.27 vs. 32.83 ± 5.9%) and bottom layer 15.97 ± 2.72% vs. 19.79 ± 3.97%); hyperactivity in the top layer (0.81 ± 0.6% vs 4.09 ± 1.98%) and the bottom layer (0.71 ± 0.68% vs. 1.50 ± 1.05%); linearity consisted of linear and non-linear, the top layer (12.19 ± 2.94 vs. 20.52 ± 3.97%) and bottom layer (12.32 ± 2.63 vs 14.70 ± 2.6); while non-linear in top layer (0.14 ± 0.2 vs 0.68 ± 0.85%) and bottom layer (0.4 ± 0.13% vs 0.34 ± 0.4%). The conclusions in this study indicated that the quality of the frozen sexed-semen that has processed by pre-freezing modification technique was better than the frozen sexed-semen obtained from the Artificial Insemination Center Standard Operating procedure.
Effect of live weight on libido, sperm quality, testosterone and lute-inizing hormone in replacement stock of Ongole Grade bull Affandhy, L.; Fitrayady, H.P.; Luthfi, M.; Widyaningrum, Y.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.352-360

Abstract

The objective of this study was to determine the effect of live weight (LW) on libido, sperm quality, testosterone, luteinizing hormone (LH) in Ongole grade (OG) bull. This study used 32 heads of OG bull with a completely randomized design that were divided to four different LW groups, i.e. group I was 170 to <210kg, group II was 210 to <250kg, group III was 250 to <290kg and group IV was 290 to 330kg. Parameters: observed were libido, testosterone hormone, LH, and semen quality. Data were analyzed by ANOVA. Libido of OG bull in group II and III higher than those in group IV. The testosterone hormone and LH showed that group II, III and IV were better than group I. The highest LH was in group IV, but low levels of testosterone were observed in these respective groups. Live sperm was the lowest in group I (21.25±13,20%) than II (66.00±11.17%), III (59.80±22.20%) and IV (75,67±9.46%). It can be concluded that the libido, sperm quality, testosterone, LH of OG bull in group II, III and IV were better than those in the group I, therefore standard LW of OG bulls had at least 250 kg.
Leptin gene polymorphism of Ongole Grade cattle based on single nucleotide polymorphism Hilmia, N.; Rahmat, D.; Dudi, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.309-314

Abstract

Point mutation on exon 2 of leptin gene, which changes amino acid encoding from Arginine to Cysteine, may alters the physiological function of the leptin hormone. This study aimed to identify leptin gene polymorphism of Ongole Grade (OG) cattle based on Single Nucleotide Polymorphism (SNP). The DNA sample was taken from 48 head of OG cattle at Balai Pengembangan Perbibitan Ternak Sapi Potong(BPPT SP) Cijeungjing West Java, which was isolated from white blood cell using the high salt method. Amplification of DNA was done by Polymerase Chain Reaction (PCR), followed by direct sequencing to obtain nucleotide sequence. The SNP analysis was carried out from alignment of sequencing result using Bioedit and MEGA 5.2 program. The results indicated in exon 2 leptin gene of OG cattle there was one synonymous SNPs that did not changeamino acids Serine encoding on g.1025T >C/S17S, while two non synonymous SNPaltered amino acids encoding, those were g.1047C> T /R25C and g.1048G>A/R25H. Those mutations changed amino acids encoding from Arginine to Cysteine and Arginine to Histidine respectively.In OG cattle, the frequency of A allele (44.8%) was higher than C allele (33.3%) and T allele (21.9%). Six genotypes were also identified, i.e. AA (41.7%), CC (20.8%), CT (20.8%), CA(4.2%), TT (10.4%) and TA (2.1 %). Heterozigosity of OG cattle based on leptin gene was 0.65 that was a high category. The A allele was a specific allele on Indonesian local cattle.
Font-matter (December 2018) Kurnianto, Edy
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.i-vi

Abstract

Ecological sustainability of smallholder dairy farm with Leisa pattern Asminaya, N. S.; Purwanto, B. P.; Nahrowi, N.; Ridwan, W. A.; Atabany, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.412-420

Abstract

This study was conducted to determine the sustainability status of smallholder dairy farms applying Low External Input Sustainable Agriculture (LEISA) pattern, and to formulate sustainable model. Eight smallholder dairy farms located in livestock business area (KUNAK) were purposive selectedwith criteria was they had 11 heads dairy cows, planted an elephant grass, dispose the manure to grassland, did not use chemicals fertilizer, 4250 m2 land area of dairy farming. Data were analyzed by: 1) attribute determination, 2) ordinal scale determination based on sustainability criteria;3) data analysis (Rap Dairy software);4) assessment of index and sustainability status;5) review sensitive attributes (leverage analysis)and 6) validation model (Monte Carloand Square Correlation (SQR) analysis). Attributes and ordinal scale on ecological sustainability weredensityof dairy cow, carrying capacity, supply water, drainage cages, soil fertility, topography, rainfall, humidity, temperature and altitude. Results of Rap Dairy analysis showed that smallholder dairy farms who apply LEISA pattern ecologically was quite sustainable with index of 57.95.The index could be increased to 88.39 (sustainable) through model simulation with respect to sensitive attributes. The results of Leverage analysis showedthat topography, densityof dairy cow, carrying capacity, supply water availability were sensitive attributes.Analysis Monte Carlo and SQR showedthat model could explain data well.

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