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Research Journal of Life Science
Published by Universitas Brawijaya
ISSN : -     EISSN : 23559926     DOI : https://doi.org/10.21776/ub.rjls
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 10 Documents
 1 
Search results for , issue "Vol 7, No 3 (2020)" : 10 Documents clear
Effect of Self-Directed Videos and Simulations on Nurse Skills in Advanced Cardiac Life Support: Comparison Study Jatim Sugiyanto; Karyono Mintaroem; Titin Andri Wihastuti
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.3

Abstract

Cardiac arrest is one of the highest causes of death in the world. This condition requires the handling of cardiac arrest based on the chain of survival concept. The chain of survival concept is carried out by nurses who have sufficient skills to handle cardiac arrest. Skills can be obtained either through education and training based on simulation or using technology such as self-directed videos. The purpose of this study was to identify the differences in self-direct video and simulations of nurse skills in advanced cardiac life support. This study used Quasi-Experimental Design by pretest-posttest with a control group approach. The 42 respondents were divided into two groups. Skills are measured before and after learning with each method. Data were analyzed using Wilcoxon and Mann-whitney tests. The results showed changes in skills before and after learning in the two methods, with each p-value = 0.000. There were no differences in skills changes before and after learning in both groups with a p-value = 0.437. Learning using self-directed videos and simulations can improve nurse skills in carrying out advanced cardiac life support.
Phenomenology Study : Family Experience in Elderly Care In Depok Jawa Barat Yati Sri Hayati; Asti Melani Astari
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.8

Abstract

The family has an important role in elderly care at home, where the majority of assistance and long-term care for the elderly is provided by the family. The nurse is responsible for assisting the family in caring for the elderly, so that in the end the family is able to provide elderly care independently at home. This study aims to explore family experiences in caring for the elderly at home. Researchers used qualitative research methods with a phenomenological approach. Data obtained through semi-structured interviews with 6 (six) participants. The research produced 5 themes: care for the elderly is a form of devotion to parents, care for the elderly requires physical and mental readiness, care for the elderly requires knowledge related to the elderly and how to care for the elderly, care for the elderly requires support from extended families, and feel the importance of supporting cadres and health workers. For conclusion, elderly families have a responsibility to meet the needs of the elderly, so that efforts are needed to improve the knowledge and skills of the family in order to properly care for and meet the needs of the elderly.
Cytochrome Oxidase C Subunit I (COI) for Identification and Genetic Variation of Loaches (Nemacheilus fasciatus) Amalia Ayuk Riyadini; Maheno Sri Widodo; Mohamad Fadjar
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.4

Abstract

Nemacheilus fasciatus is an Indonesian freshwater fish species that can be found in river waters on Java. This fish has a morphological similarity between species. This study aims to identify species of samples found and know their genetic kinship. This study used the Cytochrome Oxidase subunit I (COI) gene as a molecular marker, and then the results were analyzed using MEGA X software. The genetic structure and phylogeny of N. fasciatus sequences were combined with outgroup species from GenBank and analyzed using Maximum Likehood (ML), Pairwise Genetic Distance and Bootstrapping Phylogeny Model of Kimura 2 Parameters. The results showed that the primary pairs of LCO1490 and HCO2198 used to amplify the sample N. fasciatus with COI as a marker. The nucleotide frequencies of these loaches are A=26.5%, T=23.8%, C=19.9% and G=29.9%. The estimated Transition/Transversion bias (R) is 0.60. The number of haplotype diversity (Hd) was 0.972, and nucleotide (Pi) diversity was 0.05115. The kinship of N. fasciatus compared to the outgroup is closer to N. pallidus than N. chrysolaimos. Research needs to be conducted with a larger sample size for the genetic diversity of N. fasciatus in Indonesia.
Density and Biomass of Seagrass Beds and Its Threats on Lamongan Regency Dewi, Citra Satrya Utama; Yona, Defri; Samuel, Pratama Diffi; Maulidiyah, Rizqi Aimmatul; Syahrir, Ahdiya; Putri, Yandini Eka; Rakhmawan, Hilal; Fikri, Maulana
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.9

Abstract

This study aims to determine the density of seagrass species, seagrass biomass, and threats faced by seagrass communities in the Coastal District of Lamongan. The study was conducted in August to November 2019 in three coastal villages of Lamongan Regency, namely Tunggul Village (Station 1), Kranji (Station 2), and Banjarwati (Station 3). The stages of the study consisted of: preparation, data collection on density, threat data and seagrass stand samples, sample processing, and data analysis. The results showed that the total density of seagrass species at Stations 1, 2 and 3 was 160 stands / m2; 172 stands / m2; and 185 stands / m2. Furthermore, the total biomass of seagrass at Stations 1, 2 and 3 was 4633 gbk / m2, 2136 gbk / m2, and 9234 gbk / m2. In addition to seagrass species, seagrass density values also affect seagrass biomass. As development progresses in Lamongan Regency, it is known that the biggest threat of seagrass communities in the study site is human activity (anthropogenic), among others: anchor ships when anchored and the use of coastal areas as tourist areas, which often cleans up seagrass communities because they are deemed unfit to be enjoyed tourism sector.
Potential the Black Soldier Fly (Hermetia illucens) in Feed Formulation for Growth of Common Carp (Cyprinus carpio L.) Ayu Azkiyah Azizah; Arning Wilujeng Ekawati; Happy Nursyam
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.5

Abstract

The utilization of maggot as larvae from black soldier fly is an alternative substitution of fish meal as the primary protein source in fish feed. Maggot is cultivated in 3 different cultivation, the medium used is organic waste, tofu waste, and wheat pollard. The method that used in this research is Completely Randomized Design (CRD) with 3 treatments and 3 replication. This study aims to determine the effect of various types of maggot growth media, 1) with maggot biomass parameters, containers, and growing media conditions maggot, 2) then, exact proximate composition, total amino acids content of the prepupae samples were determined. Based on the results of the analysis, each dose of treatment (media) has an influence on the different maggot biomass. The best media treatment results are pollard flour. The proximate analysis showed that BSF larvae had a protein content of 50,88% and also fat content of 15%.
Eating Behavior of Imago Aulacophora similis Oliver on Cucumber (Cucumis sativus L.) with Treatment Plant Growth Promoting Rhizobacteria (PGPR) Dwi Wulandari; Luqman Qurata Aini; Hagus Tarno
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.10

Abstract

The cucumber harvests failure occurred mainly due to the destruction of crops by the leaf-eating beetle A. similis Oliver. Many ways are taken especially pesticides that are synthesized widely because it is considered the fastest and most potent to overcome pest disorders. Based on, one of the control techniques that can be used is PGPR, which is a group of bacteria that can be utilized to strengthen plants against pest attacks and plant diseases, so research needs to be done by using this technique to change the feeding behavior of the A. similis Oliver beetle on cucumber plants. The research was conducted at the Green House Department of Pest and Plant Diseases University of Brawijaya Malang in September 2018 to March 2019. It was conducted using Randomized Block Design (RBD) 5 treatment, i.e., PGPR, urea fertilizer and control (without treatment). Based on the results of the study shows that: Application of PGPR on cucumber plant can reduce damage on either leaves or flower due to the eating behavior of imago A. similis Oliver and Application of PGPR can increase the cucumber plant growth during the eating behavior of imago A. similis Oliver.
Effect of Centella asiatica Extract on Locomotor Activity and Hsp60 Expression in Zebrafish models of Parkinsons Rodhiyan Rakhmatiar; Mulyohadi Ali; M. Dalhar
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.1

Abstract

Parkinson's disease characterized by a decrease in motor activity is a progressive neurodegenerative disorder caused by the degeneration of dopaminergic neurons. Centella asiatica is suspected of having a neuroprotectant effect and is not yet known how Centella asiatica role in the prevention of Parkinson's disease. The study was conducted to prove the effect of Centella asiatica extract on the expression of Hsp60 and locomotor activity zebrafish models of Parkinson's with rotenone exposure. The study was conducted using 25 zebrafish divided into various groups. Centella asiatica extract and rotenone exposure have given for 28 days, observed locomotor activity on days 0, 7, 14, 21 and 28. The expression of Hsp60 measured using immunohistochemical techniques. There is a significant difference between locomotor activity at various doses of Centella asiatica (p<0.05) with a very strong correlation (r=0.929; p<0.01) where the higher doses of Centella asiatica, the higher locomotor activity. Found a significant difference between the reduced expression of Hsp60 to various Centella asiatica dose group (p<0.05) but no correlation between the expression of Hsp60 with Centella asiatica dose groups and locomotor activity. Centella asiatica extract is able to increase locomotor activity and decrease the expression of Hsp60 in zebrafish models of Parkinson's.
The Role of Zinc Supplementation in The Lung Tissue Damage of Tuberculosis Rats Sukma Sukma Sahadewa
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.6

Abstract

Infection by Mycobacterium tuberculosis (Mtb) is a chronic contagious disease with a high mortality rate in various parts of the world, especially in developing countries with high poverty rates. The objective of the present study was to examine the effects of zinc supplementation on the lung tissue damage of the male tuberculosis rats. This study was conducted based on true experimental design, using a post-test control group design. Peribronchiolitis was found to have a higher rank in (K0, K1, K2) groups compared to the Mtb group treated with the addition of Zinc supplements (P1, P2, P3). Zinc supplementation in this study shows that there is a reduction in lung organ damage and having a positive impact on increasing the perivasculitis and granuloma. Zinc supplementation by its optimal dose of 50 mg/kg.bb/day given as additional nutrition in Mtb rats.
Correlation Analysis of Knowledge and Education Against the Implementation of Basic Life Support (BHD) in Patients of Cardiac Arrest by Nurses in Emergency Installation and ICU of General Hospital of Dr. Soedarso Pontianak Maulidah Maulidah; Dhelya Widasmara; Titin Andri Wihastuti
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.2

Abstract

Cardiac arrest is a condition where the heart stopped abruptly, and this condition usually occurs in someone who has had heart disease or has never experienced it. In terms of clinical, someone who is experiencing a state of cardiac arrest found no signs of a palpable pulse and other signs of circulation. Efforts in terms of handling cases of the cardiac arrest itself need the role of nurse, one cardiac arrest treatment efforts by nurses are implementing Basic Life Support. The factors that affect the implementation of BHD itself is knowledge and education. The purpose of this research is analizing the correlation between knowledge and education on the implementation of BHD in cardiac arrest patients by nurses in the Emergency Installation and ICU of the General Hospital of Dr. Soedarso Pontianak. This study uses a cross-sectional approach with descriptive corelational type. The population in this study are 56 nurses. Sampling technique using total sampling. The result of this study, based on two variables: the knowledge and education have the same p-value that is p=0.000 or α <0.05. It means that there is a correlation between knowledge and education on the implementation of BHD in patients with cardiac arrest in General Hospital of Dr. Soedarso Pontianak.
The Effect of Pregelatinization with Heat and Moisture Treatment on Physicochemical and Pasting Characteristics of Red Glutinous Rice Flour Jhauharotul Muchlisyiyah; Hera Sisca Prasmita; Teti Estiasih; Ratna Palupi Nurfatimah
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.7

Abstract

Red glutinous rice is native from Pacitan and is still limited to local region consumption. The color of the rice flour showed that it has bioactive content, which is the potential to wider the use. The effect of pregelatinization using heat and moisture treatment by boiling on physicochemical (starch, amylose, anthocyanin, gel consistency, Lab color, SEM figure) and pasting properties of red glutinous rice flour was determined. The time and temperature of pregelatinization were 5, 10, and 15 minutes and 50°C, 60°C, and 60°C. The experiment was done with a completely randomized design with three replications. Starch content range to 62-77%, amylose content 3.29-5.75%, anthocyanin 2.64-6.71 mg/100g, Moisture 6.16-7.45, L 72.5-73.95, redness 5.11-6.00, yellowness 8.87-9.62, and gel consistency 9.7-12 cm. the increase of time and temperature of pregelatinization lead to decrease of amylose, starch, and anthocyanin content, and viscosity. After pregelatinization, the setback viscosity was lower it means that the rate of retrogradation is lower than native starch. SEM figure also showed that pregelatinization using heat and moisture treatment did not destroy the starch granules.

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