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Natural Science: Journal of Science and Technology
Published by Universitas Tadulako
ISSN : 25411969     EISSN : 23380950     DOI : -
Core Subject : Science,
Computer Science & IT
Jurnal Natural Science online diterbitkan oleh Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) Universtas Tadulako. Jurnal ini menerbitkan artikel-artikel ilmiah hasil penelitian dalam bidang-bidang ilmu pengetahuan dan teknologi.
Arjuna Subject : -
Articles 11 Documents
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Search results for , issue "Vol 1, No 1 (2012)" : 11 Documents clear
ANALISIS STRUKTUR BAWAH PERMUKAAN DENGAN MENGGUNAKAN METODE SEISMIK REFRAKSI DI UNIVERSITAS TADULAKO Adnyawati, Ni Ketut; Efendi, Rustan; Sabhan, Sabhan
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Abstract

The method is one of the seismic refraction geophysical methods that can be used to determine the structure of the subsurface. The purpose of this study was to to determine the subsurface structure in the area Tadulako University. In this method, the waves generated on the surface of the Earth that will reach down into the surface of the soil or rock, then disuatu point of the boundary between two layers of rock wave is reflected back to the surface and partially transmitted down some refracts due to changes in rock density. Wave propagation below the surface will be detected by the geophones. Aqusisi and data processing method Intercept Time. Data processing is done in 2 stages, using the program and program pickwin Plotreva. The end result of this data processing is the image of the subsurface profile Tadulako University of 6 stretch consisting of three layers of the same. The first layer has a wave speed (V1) 300 m/s are expected mixing of sand gravel dominated by gravel, the second layer has a wave velocity (V2) 1000 m/s are suspected of mixing sand gravel dominated by sand and the third layer has a velocity 2000 m/s are expected mixing of sand and clay.
PERBANYAKAN TANAMAN MELON (CUCUMIS MELO L.) SECARA IN VITRO PADA MEDIUM MS DENGAN PENAMBAHAN INDOLE ACETIC ACID (IAA) DAN BENZIL AMINO PURIN (BAP) Lidyawati, Ni Nyoman; Waeniati, Waeniati; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Abstract

The research on organogenesis of melon (Cucumis melo L.) was done at Tissue Culture Laboratory Forestry Faculty UNTAD Palu, during January until May 2012. The aim of this research was to determine the best concentration of IAA and BAP hormones in inducing organs of this plant.  Sterile seedling of melon was used as explants in this work. The experiment was arranged in  Completely Randomized Design (CRD)  with  4 treatments and 4 replications. The treatments were MS0 + 0,1 ppm IAA + 0,5 ppm BAP (M1), MS0 + 0,1 ppm IAA + 0,7 ppm BAP (M2), MS0 + 0,1 ppm IAA + 0,9 ppm BAP (M3) and MS0 + 0,1 ppm IAA + 1 ppm BAP (M4). Parameters observed on this study were the day appear of shoot and leaves, the number of shoots and leaves and also the present of callus and root. The result showed that the best medium for organs induction was MS0 + 0,1 ppm IAA + 1 ppm BAP, which shoot and leaf emerged in the day of 3,75 and 5,75 after induction, respectively. This media was also induced the number of shoots and leaves, i.e 4,75 and 8,75, respectively. Nevertheles this media was not suitable enough in inducing root formation.
OPTIMALISASI HIDROLISIS SUKROSA MENGGUNAKAN RESIN PENUKAR KATION TIPE SULFONAT Razak, Abd. Rahman Razak; Sumarni, Ni Ketut; Rahmat, Basuki
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Abstract

Optimization of sucrose hydrolysis has been investigated by using a sulfonic type cation excange. The aim of investigation are to find out the effect of resin concentration, agitation time and the recycling of resin without ion excange treatment with optimum condition for the effectiveness of resin as catalys in hydrolysis of sucrose 70%. The result, there were obtained that the optimum condition in hirolysis of sucrose occurred at 30 grams of resin and agitation time at 16 hours, the product of sugar invert was 833.810 mg or 1.132%. The recycled of resin without ion treatment on the optimum condition as catalys in hidrolysis sucrose affected to the reduction of resin effectiveness. From Duncan?s test, the best effectiveness recycling of resin without ion treatment on the optimum condition are four time
PENGARUH PENAMBAHAN AIR KELAPA DAN BERBAGAI KONSENTRASI HORMON 2,4-D PADA MEDIUM MS DALAM MENGINDUKSI KALUS TANAMAN ANGGUR HIJAU (VITIS VINIFERA L.) Dwi PYD, Niluh Made; Waeniati, Waeniati; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Abstract

Green grape (Vitis vinifera L.) is popular cultivar of grape that produce valuable of table fruit whic usually grown via vegetative propagation. Another  chalanging tecnology in grape propagate is by induction of callus in tissue culture tecnique. The study on grape callus induction was done at Tissue Culture Laboratory-Forestry Faculty-Tadulako University Palu, during periode of January 2012 until May 2012. This experiment was arranged in completely randomized design with 3 different mediums as treatments and 3 replications. The treatments were MS0 + 2,4-D 0.5 ppm + 10% coconut water (D1), MS0 + 2,4-D 1 ppm + 10% coconut water (D2), dan MS0 + 2,4-D 1.5 ppm + 10% coconut water (D3). The observed parameters in this research were the days appear of callus, percentage of explant producing callus (%), also observation on morphology and the cell of callus. The result showed that the MS medium with 10% coconut water and 2.4-D could induce the callus of the green grape and the best medium was MS0 + 2.4-D 1.5 ppm + 10% coconut water (D3). It was shown by responding in emerging of callus in 11 days after induction, the percentage of explant producing callus was 76.67%. Produced callus was active in cell proliferation,  and it has a compac texture, with brown-greeny color of callii.
KARAKTERISASI ENZIM AMILASE DARI KECAMBAH BIJI JAGUNG KETAN (ZEA MAYS CERATINA L.) Bahri, Syaiful; Mirzan, Moh.; Hasan, Moh.
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Abstract

The research about characterization of the amylase enzyme from germination glutinous corn seed (Zea mays ceratina L.) was done. The aims of this research is to know the effect of germination time to the activity of the amylase enzyme and characteristics of the amylase enzyme from germination glutinous corn seed (Zea mays ceratina L.). The research was conducted through four phases that is germination stage, extraction, isolation, and characterization. The research device used was Completelly Randomized Design (CRD) and to be continued by BNJ test critical level 5%. Result showed that the germination time that produces the highest activity was 36 hours and the activity of enzyme was 0.0557 sec-1. Isolation of the amylase enzyme purified by salting out method used technical (NH4)2SO4 at concentration 55% with activity of 0.0394 sec-1. The optimum pH was 9, and the activity of enzyme was 0.0207 sec-1. Maximum substrate concentration with the activity 0.0476 sec-1 give at 12.5% (w/v), while the optimum temperature for activity 0.0229 sec-1 was 70 oC. From examination manner analysis for all of free variables results very real effect on the amylase enzyme activity, while the BNJ test critical level of 5% showed a real difference with the other treatments of every variable.
IDENTIFIKASI JALUR PATAHAN DENGAN METODE GEOLISTRIK HAMBATAN JENIS DI WILAYAH PALU BARAT Fitriani, Zelly Rizky; Rusydi H., Muhammad; Th Musa, Moh. Dahlan
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Abstract

Research identify fault lines with resistivity geoelectric measurements in West Palu has been done. This study aimed to determine the geological structure, and also the type of rock layers below  the  surface  area  of  research.  This  measurement  was  carried  out  in  two  areas  in  the western district of Palu, the Kalora and Kabonena, using geoelectric methods resistance type with Wenner configuration. Data processing was done using software EarthImager 2D. From the results of treatment, obtained a description of the geological structure in line 2 at Kalora areas there resistance values between 1212-6000 ohm-m which allegedly was granite layer that  separated  by  a  layer  of  sandy  loam  with  a  resistance  value  of  200-1000  ohm-m  in coordinates of 119 °49'33,7? BT and 00 ° 52'42, 5" LS, which was characteristic of the fault.
ORGANOGENESIS TANAMAN ANGGUR HIJAU (VITIS VINIFERA L.) PADA MEDIUM MSDENGAN PENAMBAHAN IAA (INDOLE ACETID ACID) DANBERBAGAI KONSENTRASI BAP (BENZIL AMINO PURIN) Tajuddin, Riska; Suwastika, I Nengah; Muslimin, muslimin
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Abstract

Research onorganogenesisof Grape (Vitis viniferaL.) on MS medium supplemented with IAA and various sconcentrations of BAP, was conducted at the Tissue Culture Laboratory, Faculty of Forestry Untad Palu, during periode of Januari and April 2012.This research was aimed to obtain the best combination ofIAA and BA Pin MS medium that stimulate organogenesis of Grape. This experiment was arranged in completely randomized design with 4 treatments and 3 replications.The treatmentswere: MS medium+ 0.1ppm IAA+ 0.1 ppm BAP(B1), MS medium+ 0.1 ppm IAA +0.3 ppm BAP (B2), MS medium + 0.1ppm IAA + 0.5ppm BAP(B3) and MS medium+ 0.1 ppm IAA+0.7BAP(B4). Parameters observed in this study were the days appear of shoots, the days appear of leaves, percentage of explant producing shoots (%), and the presence or absence of roots. The results showedthat MS medium+ 0.1 ppm IAA+0.7ppm BAP(B4) gived the best results in speedupof shootsandleaves,in 7 days after induction.
PERTUMBUHAN ORGAN TANAMAN BUAH NAGA(HYLOCERUS UNDATUS) PADA MEDIUM MS DENGAN PENAMBAHAN BAP DAN SUKROSA Sulistiami, Ari; Waeniati, Waeniati; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Abstract

Dragon fruit (Hylocyrus undatus) is a commercial-cultivated plant that has a high economic value and believed to be a healthy-food. Propagation of seedling by tissue culture technique is challenging step during cultivation of these plant. This research was aimed to determine the efeect of sucrose and BAP concentration in the dragon fruit (H. undatus). This experiment was arranged in completely randomized  design with 4 treatments and 3 replications. The treatmnts were : MS0 + 0,5 ppm BAP + 35 g/L sucrose (A1), MS0 + 0,5 ppm BAP + 405 g/L sucrose (A2), MS0 + 0,5 ppm BAP + 45 g/L sucrose (A3), and MS0 + 0,5 ppm BAP + 50 g/L sucrose (A4). The results showed that all medium tested were suitable medium in inducing organs, including emerge of buds, thorns and roots. The best medium in inducing organogenesis of the plant were MS0 + 0,5 ppm BAP + 50 g/L sucrose.
INDUKSI KALUS TANAMAN KAKAO (THEOBROMA CACAO L.) PADA MEDIA MS DENGAN PENAMBAHAN 2,4-D, BAP DAN AIR KELAPA Ariati, Sri Niken; Waeniati, Waeniati; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Abstract

Study on callus induction of cacao (Theobroma cacao L.) was conducted at the Tissue Culture laboratory, Forestry faculty, Tadulako University, Palu, during periode of January until April 2012. This research was aimed to obtain the best medium formulation for induction and growth of cacao callus. Calli was induced from embryo  on MS based medium containing of 2,4-D, BAP and coconut water. The intact plant as explant was taken from Local Farming. This experiment was arranged in completely randomized  design with 4 treatments and 3 replications. The treatments were : MS medium + 2 ppm 2,4-D + 15% coconut water (N1), MS medium + 2 ppm 2,4-D + 0,2 ppm BAP, 15% coconut water (N2), MS medium + 2 ppm 2,4-D + 0,2 ppm BAP (N3), and MS medium + 2 ppm 2,4-D (N4). Parameters observed in this study were the days appear of callus, percentage of explant producing callus (%), also observation on morphology and the cell of callus. The best medium for callus  induction was MS medium + 2 ppm 2,4-D + 15% coconut water (N1). This medium produces healthy-compac callus which active in cell proliferation, in 6 days after induction.
ORGANOGENESIS TANAMAN JERUK KEPROK (CITRUS NOBILIS LOUR.) SECARA IN VITRO PADA MEDIA MS DENGAN PENAMBAHANBERBAGAI KONSENTRASI IAA (INDOLE ACETID ACID) DAN BAP (BENZYL AMINO PURIN) Harliana, Harliana; Weaniati, Weaniati; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Abstract

Research on"Organogenesis of Tangerine orange (Citrus nobilisLour) onMSmediumsupplemented withvariousconcentrations ofIAAandBAP," has been performedatthe Tissue CultureLaboratory ofthe Faculty ofForestry, Tadulako University, Palu during the period of January andMay 2012. The goal of this study was to determine the optimum growth factor combination of IAA and BAP in MS based medium, for organogenesis of the plant. This research was arranged on Completely RandomizedDesign(CRD) withsix treatments and three replications. Growth factor combinations tested in this study were:  0.1ppm IAA + 0.4ppmBAP(C1), 0.1ppm IAA + 0.6ppm BAP(C2), 0.1ppmIAA+0.8ppmBAP(C3), 0.1ppm IAA + 1.0ppmBAP(C4), 1.0ppm IAA + 0.6ppmBAP(C5), and1.0ppmIAA+1.0ppmBAP(C6). The result indicating that the best organogenesis of Orange explant was in MS medium supplemented with 0.1 ppm IAA and 1.0 ppm BAP (C4). This medium was suitable in inducing shoot and leaf, faster than its in other medium. Explant on this medium was also produced higher number of leaves than it on other treatments.

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