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Biocelebes
Published by Universitas Tadulako
ISSN : -     EISSN : 25805991     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 303 Documents
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Penerapan Sistem Manajemen Kesehatan dan Keselamatan Kerja di Rumah Sakit Bersalin Pertiwi Makassar Nur Asmar Salikunna; Vera Diana Towidjojo
Biocelebes Vol. 5 No. 1 (2011)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The hospital is a health facility which organizes the activities of health services and can serve place of health education and research.The hospital has special characteristics that can increase the chances of accidents thus requiring theapplication of Health and Safety at Work management system as regulated in Law No. 23 of 1992 on Health,Article 23 stated that Health and Safety at Work efforts be held in all workplaces, especially workplaces that have a health hazard, easily affected by the disease or have employees at least 10 people.This research was this Research used a survey method with a descriptive approach to seeing the implementation Health and Safety at Work Management system at RS.Bersalin Pertiwi Makassar.The results showed that the achievement of Health and Safety at Work Management system in RS.Bersalin Pertiwi Makassar amounted to 57.86% (81 criteria applied).So this hospital into the range of levels of achievement of the application of 0-59%, so the entry in the poor category.
Keragaman Karakter Fenotipik Nyamuk Aedes di Daerah Endemis Demam Berdarah di Kalimantan Barat Kustiati Kustiati; Siti Ifadatin; Syarief Adiansyah
Biocelebes Vol. 5 No. 1 (2011)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research on variety of phenotypic characters Aedes mosquito in dengue fever endemic area in West Kalimantan was conducted on July until December 2008. The research site ware fixed according to Stratified Random Sampling Method. The research was conducted by collating the morphological characters based on form, colour and amount of head, thorax and abdomen. Analysis of cluster using PCA (Principal Component Analysis). The inventory result found four species Aedes mosquito that are A. aegypti, A. albopictus, A. polynesiensis, and A. scuttelaris. Based on principal component analysis each mosquito larva, male and female have 9, 18 and 17 characters respectively determind for those four species of Aedes.
Tingkat Keganasan Saprolegnia parasitica Pada Ikan Patin (Pangasius hypophthalmus Sauvage) dan Tindakan Kuratif Alaminya dengan Lactobacillus plantarum Atira Atira
Biocelebes Vol. 5 No. 1 (2011)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research of S. parasitica parasitic on cathfish that would inhibit mycelium growth with L. plantarum conducted according to in vivo and in vitro. This research conducted to find out infection concentration of S. parasitica on catfish, and research L. plantarum concentration that inhibit or cure parasitic disease of S. parasitica on cathfish. This research consist of two steps, first step that is concentration of zoospore suspension count that cause 50% infection on catfish. Secondly step that is ability test concentration variation of L. plantarum within inhibit parasite on cathfish. First research step conducted according to experimental laboratory, that is REED-MUENCH method was used to determine IC50. Observed parameter was number of infected catfish by S. parasitica, and its infection diameter. Second step that is curative test was conducted from infected catfish by S. parasitica with L. plantarum inoculums. Used design was Completely Randomized Design with factorial pattern that was 4 x 4, with first factor consisted of 4 infection concentration of S. parasitica suspension, i.e. 0xIC50, IC50 (Infectious Concentration 50%), 2xIC50, and 4xIC50. Second factor was consisted of 4 concentration of L. plantarum FNCC 226 inoculums, i.e. MaxNLC (Maximal Nir Lethal Concentration), ½xMaxNLC, ¼xMaxNLC, dan 0xMaxNLC, with 4 times replication. Observed parameter was comparison of infection diameter of S. parasitica on catfish before and after curative concentration giving of L. plantarum inoculums. First step research result about IC50 count showed that infection concentration for 50% was are 1 x 107 zoospore/mL. Increment of zoospore concentration was along with increment of infection diameter on cathfish. Second step research result about curative, that L. plantarum 7,7 x 105 cfu/mL (MaxNLC), 4,0 x 105 cfu/mL (½xMaxNLC), 2,3 x 105 cfu/mL (¼xMaxNLC) could inhibit mycelium of S. parasitica. Decrement of S. parasitica infection, was along with increment of L. plantarum capacity blocked. Mycelium diameter of S. parasitica decrement very real after giving L. plantarum.
Pengaruh Konsentrasi Larutan Sukrosa dan Waktu Perendaman Terhadap Kesegaran Bunga Potong Oleander (Nerium oleander L.) Eny Yuniaty; Muhammad Alwi
Biocelebes Vol. 5 No. 1 (2011)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The study of "The Effect of Solvent Preservative Against Security To Save Oleander Flowers (Nerium oleander L.)" was held from March to April 2011 which aims to identify and study the effect of preservative solutions on postharvest quality of cut flowers Oleander (Nerium oleander L.). The research method used is an experiment with 14 treatments and 3 replications. The design used was the design (CRD) with factorial pattern factor of sucrose concentration and immersion time. The results showed that the resistance to keep, the number of blooming flowers, flowers wither, and the volume of water absorption is best at a concentration of 5% with 2 hours soaking time compared with other treatments.
Dampak Perubahan Hutan Menjadi Kebun Kakao Terhadap Keanekaragaman Laba-Laba (Araneae) di Sulawesi Tengah Shahabuddin Shahabuddin; Abd. Wahid Abd. Wahid; Elijonnahdi Elijonnahdi
Biocelebes Vol. 5 No. 1 (2011)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

This study aims to evaluate the effect of forest conversion to agriculture, especially cocoa plantation on spider diversity in Central Sulawesi. The experiment was conducted in the vicinity of the village of Bobo Palolo Subdistrict, Donggala in the margins of Lore Lindu National Park in Central Sulawesi. Spiders were collected on transects size of 50 mx 10 m at each study site using insect nets. The results showed that there are at least 17 species from 11 families of spiders recorded from natural forest, disturb forest, and cacao plantation. Changes in habitat type affect the diversity of spiders. Spider species diversity tends to decline due to habitat change from natural forest to cocoa plantations. However the forest with intermediate level of disturbance as well as a cacao plantation that maintains many types of shade trees has capability for supporting spiders diversity. Community structure of spiders at the cacao plantation are relatively similar to those in natural forests.
Kajian Beberapa Aspek Botani Anggrek Endemik Coelogyne celebensis J.J. Sm. dari Taman Nasional Lore Lindu Sulawesi Tengah Ramadhanil Ramadhanil
Biocelebes Vol. 4 No. 1 (2010)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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A research about the study on some botanical aspects of an endemic orchid Coelogyne celebensis J.J.Sm from Lore Lindu National Park has been conducted from March to November 2009. The research was located at the western part of Lore Lindu National Park, Herbarium Celebense (CEB) UNTAD, Orchid house of Bogor Botanic Garden, and the Herbarium Bogoriense (BO) Cibinong West Java. The survey method in the field was used to collect the living material of Coelogyne celebensis J.J.Sm, and then the description of the orchid was done at the Herbarium Celebense (CEB), Herbarium Bogoriense and Bogor Botanic Garden. The data of some botanical aspects included morphological characteristics, ecological (bitoik and abiotik) factor and social economic of the community who living around the habitat of the orchids were presented. Key words: Endemic orchid, botanical aspect, Coelogyne celebensis J.J.Sm, Lore Lindu NP.
Keanekaragaman Zooplankton di Perairan Kepulauan Togean Kabupaten Tojo Una-Una, Sulawesi Tengah Miswan Miswan
Biocelebes Vol. 4 No. 1 (2010)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The structure of plankton community have been conducted in Togean Island from September 2008 untill Februari 2009, it used descriptive method. The result of the structure of zooplankton community indicate plankton range from 0,862-0.879, with value of biodiversity range from 0.328-0.330, for the value of variety of fitoplankton range from 0.328-0.329 and found marozoobenthos range from 26-27 individual/L, with variety value of benthos range from 0.831-0.835 with value of biodiversity range from 0.339-0.341. This indicator is not indicate the ecology pressure, but is not depend on river condition. Key words: Biodeversity, zooplankton, and Togean.
Evaluasi Efek Terapeutik Terhadap ISPA Serta Status Gizi Anak Balita M. Sabir; Sarifuddin Sarifuddin
Biocelebes Vol. 4 No. 1 (2010)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The study is devoted to investigating the therapeutic effect on severity of acute respiratory infection (ARI) and nutritional status of underfive years old. It is analytic design of evaluative by visiting the house of sample at the fourth until the ninth days after giving therapeutic. The number of sample was 50 under five years old, selected by accidental sampling. The data were analyzed with wilcoxon sign runk test and paired t-test. The study indicated that first diagnoses of acute respiratory infection severity, it was found that average of duration of ARI was 3 days (17%), severe ARI is 84% and mild ARI 16%. Based on nutritional status (weight/age = W/A), severe malnutrition (8%), undernutrion (16%), and well nutrition (76%). The fourth days of therapy was found that duration of ARI was six days (66%) and severe ARI (52%), mild ARI (32%) and healing (16%). Meanwhile, nutritional status was severe malnutrition (6%), undernutrition (20%) and well nutrition (20%). The nine days of therapy, duration of ARI was six days (56%), severe ARI was 20%, mild ARI was 34% and healing was 46%. Severe malnutrition was 6%, undernutrition was 18%, and well nutrition was 76%. Paired T-test and Wilcoxon test, shown that there was therapeutic effect on duration and severity of ARI (P = 0,000) and (P = 0,004), and no effect of nutritional status based on paired t-test (P = 0,748). The conclusion is therapeutic can reduce of duration and severe of ARI, meanwhile nutritional status dose not have effect. Key words: Therapeutic effect, severity of ARI, nutritional status, underfive years old.
Analisis in-Silico pGEM-GLL Recombinan Glukoamilase Saccharomycopsis fibuligera R64 Pasjan Satrimafitrah
Biocelebes Vol. 4 No. 1 (2010)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Saccharomycopsis fibuligera is yeast with high amylolitic activity. The local strain, S. fibuligera R64 has been reported to produce starch hydrolyzing enzymes, -amylase dan glucoamylase. Glucoamylase is one of the extracellular enzymes that enable to breakdown starch in starch processing, thus has potential to be developed and applied in starch processing industry, such as glucose syrup production. Wild type microorganism-producing glucoamylase normaly express low level of enzyme, while high level production and low cost enzymes are needed in many industries. To obtain strains with superior properties, a recombinant DNA is used and Pichia pastoris is a suitable expression host for producing glucoamylase at high level. Gene encoding glucoamylase S. fibuligera R64 (GLL) has been amplified and cloned into pGEM-T vector. A set of primers (Glu-F and Glu-R) was designed based on the published nucleotide sequence (Acc. No M17355). Restriction analysis of pGEM-GLL by using EcoRI and XhoI showed a ~4.500 kb DNA fragment which confirmed the size of pGEM-T vector containing GLL. This research aimed to analyze pGEM-GLL S. fibuligera R64 in silico. Gene alignment of GLL S. fibuligera R64 with GLU1 S. fibuligera showed 99 % homology. Based on in silico translation analysis, there were several nucleotide difference compared to the published sequence which result in amino acid differences, namely E27D, N46D, dan A376V. Hence it is possible that S. fibuligera R64 has different properties from other known S. fibuligera. Key words: Glucoamylase, S. fibuligera, E. coli TOP10F’, PCR, in silico analysis.
Potensi Simpanan Karbon Berdasarkan Struktur Tinggi Tanaman Pola-Pola Agroforestry di Kecamatan Tinggimoncong dan Parigi Kabupaten Gowa, Sulawesi-Selatan Syamsuddin Millang
Biocelebes Vol. 4 No. 1 (2010)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The study is aimed to (1) identify agroforesry pattern inside and outside of forest area based on structure and species composition; (2) know carbon stocks potential of agroforestry pattern base on vertical structure; (3) estimate aboveground carbon stocks of agroforesry patterns. The study was conducted on May to September 2009 in Tinggimoncong and Parigi District, Gowa Regency and Laboratory of Silvikultur, Hasanuddin University. To know tree biomass, measurement of diameter breast height was carried out. Total number of sample plots taken was 37 with 20 x 50 m in size. Selection and placement of sample plot was conducted by Purposive Sampling based on consideration of tree species dominant on each different agroforestry patterns. Withdrawal of tree’s biomass samples was conducted by non-destructive sampling, namely without destructing samples. The study results showed that (1) agroforestry system which generally used by community at the study location was agrisilviculture consisting of nine combination’s pattern of crop species. Interval adjustment follows random and border pattern with crop interval of tree component were irregular, (2) number carbon-stock on every range of vertical structure of agroforestry patterns were varied. Agroforestry Erythrina-jackfruit-coffee, clove-annual crops, african tree-coffee, Gmelina-banana patterns tend to follow bell-shaped curve or the stored carbon was the largest on medium-diamater trees, pine-vegetable, Eucalyptus-vegetable crops, pine-coffee, fruit trees-coffee tend to follow J-shaped curve or the stored carbon was the largest on wider-diameter trees, and agroforestry Erythrina-coffee patterns tend to be irregular; (3) two agroforestry patterns which had the highest potential carbon stocks aboveground were pine-coffee and fruittree-coffeeI agroforestry pattern amounted of 199.97 ton ha-1 and 100.54, respectively, and the lowest agroforestry pattern was clove-annual crop pattern namely 18.40 ton ha-1. Key words: Carbon stocks, agroforestry pattern, vertical stratification.

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