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Ivan Permana Putra
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Jurnal Sumberdaya Hayati
Published by Institut Pertanian Bogor
ISSN : -     EISSN : 2477037X     DOI : DOI: 10.29244/jsdh.9.1.7-16
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
The "Jurnal Sumberdaya HAYATI" is a peer-reviewed and an open access journal published by Department of Biology, Bogor Agricultural University, Indonesia. The journal publishes significant and important research from all area of biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 153 Documents
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Formula Media Pertumbuhan Bakteri Asam Laktat Pediococcus pentosaceus Menggunakan Substrat Whey Tahu SAFITRI NURLAELA; TITI CANDRA SUNARTI; ANJA MERYANDINI
Jurnal Sumberdaya Hayati Vol. 2 No. 2 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.2.31-38

Abstract

The lactic acid bacteria (LAB) and its lactic acid are commonly used to preserve the food and to extend the food’s shelf life. MRS media is a growth medium for LAB, but it is not feasible for industrial scale application. Cheaper substrate from agriculture products is therefore required, such as tofu whey, which is potential to be used as LAB medium. Tofu whey contains important components to support the LAB growth, but it needs C source (5% of glucose) and nitrogen source (1% ammonium sulphate or urea) supplementations. This study aimed to investigate the influence of N-source to Pediococcus pentosaceus growth and its capability in producing acid compounds. The result showed that addition of urea increased pH fermentation, contrarily to that ammonium sulphate supplementation. The highest bacterial growth rate (μmaks) was observed on media with urea (0.43 jam-1), while the highest acid production was occured on media with ammonium sulphate (9.13 mg/mL). Supplementation of ammonium sulphate and urea on tofu whey highly supported the growth of bacterial population for about 6.5 × 108 CFU/mL and 5.4 × 108 CFU/ mL, respectively, but still lower compared to MRS media (2.03 × 1010 CFU/mL). 
Potensi Seduhan Limbah Baglog Jamur Tiram (Pleurotus ostreatus) dalam Menekan Populasi Radopholus similis dan Meloidogyne spp. pada Tanah Asal Perakaran Tanaman Lada (Piper nigrum L) ANKARDIANSYAH PANDU PRADANA; DIANA PUTRI; ABDUL MUNIF
Jurnal Sumberdaya Hayati Vol. 2 No. 2 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.2.39-44

Abstract

Indonesia is one of the largest pepper producing countries in the world. One of the pepper-producing provinces in Indonesia is the Bangka Belitung Islands (Babel). However, the infection of Radopholus similis and Meloidogyne spp. which causes yellow disease becomes one of obstacles in pepper cultivation in Babel. Thus, alternative solution to overcome this yellow disease is by reducing the amount of nematode inoculum of R. similis and Meloidogyne spp. in soil. This study aimed to determine the effectiveness of the infusion of oyster mushrooms baglog waste enriched with molasses to control the population of R. similis and Meloidogyne spp. and to increase the population of rhizobacteria. Infusion of oyster mushrooms baglog waste was mixed with 1% of molasses and was poured into soil obtained from the pepper plant roots. The concentrations used were 10%, 20%, 30% and 50%. Population of phytonematode, rhizobacteria, proteolytic bacteria, and group of fluorescence bacteria were calculated before and 7 days after treatment. Results showed that the populations of R. similis and Meloidogyne spp. in the soil poured with the influsion of oyster mushroom baglog waste decreased by 29.11% (R. similis) and 24.61% (Meloidogyne spp.), compared to the before and control treatments. The suppression of nematode population was found to be the highest in the influsion treatment at concentration of 50%. Overall, treatment of all concentrations succeeded to increase the population of rhizobacteria, proteolytic bacteria, and group of fluorescence bacteria in soil. Moreover, the highest increase was found in soil treated with concentration of 50%. This study provided new information that the infusion application of oyster mushrooms baglog waste enriched with molasses had the potential to increase the population of rhizobacteria and suppress the amount of pathogens R. similis and Meloidogyne spp. 
Biologi Penggerek Batang Jagung Ostrinia furnacalis Gueneé yang diberi Pakan Buatan ARIF MUKHTAR HASBI; RIKA RAFFIUDIN; I MADE SAMUDRA
Jurnal Sumberdaya Hayati Vol. 2 No. 1 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.1.13-18

Abstract

Ostrinia furnacalis is a corn stem-borer that develops complete metamorphosis and all stages in life cycle in corn. This research was aimed to examine several biology aspects of O. furnacalis such as life cycle, egg incubaton period, egg fertility, female fecundity, longevity of imago, and copulation time on artificial diet, based on the previous study. The results of the observations showed that the life cycle of artificial-diet-given O. furnacalis was between 27-34 days range. Female fecundity was 16-452 eggs with fertility rate of 61,97% and 3-5 days renge of egg incubation period. Longevity of imago was between 6-11 days range, and the longevity was longer in female compared to the male. The imago of O. furnacalis copulate on 0-3 days after emerge from pupae and the highest number in on the day 1. Copulation time was occurred at 3-8 hour after scotophase commenced and the highest was at third hour. The artificial diet used in this research can be used for O. furnacalis mass rearing purpose and performed shorter length of egg stage until pupal stage compared to mass rearing with natural diet.
Penapisan Bakteri Filosfer Penghasil Senyawa Bioaktif Anti Xanthomonas oryzae pv. oryzae Penyebab Penyakit Hawar Daun Bakteri pada Padi NURFITRIANI RINA; NI PUTU RATNA AYU KRISHANTI; ALINA AKHDIYA; ARIS TRI WAHYUDI
Jurnal Sumberdaya Hayati Vol. 2 No. 1 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.1.19-24

Abstract

Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the important diseases in rice crops in Indonesia. The disease is difficult to be controlled since it attacks the rice plant at different growth stages such as tillering, flowering and ripening. One of the alternatives that could be used to control the disease is by using phyllosphere bacteria as the biocontrol agents. This study aims to isolate, characterize and screen the rice phyllosphere bacteria producing bioactive compounds against Xoo. Phyllosphere bacteria isolated from healthy leaves of rice var. Ciherang by using 4 different media obtained 285 bacterial isolates which were consisted of the 65 isolates of King’s B agar, 86 isolates of Nutrient agar, 81 isolates of Luria-Bertani agar, and 53 isolates of Trypticase Soy agar media. Antagonist test using double layer method showed 58 isolates of phyllosphere bacteria produced bioactive compounds that inhibited the growth of Xoo. Pathogenicity test agaist rice leaf revealed 18 bacterial isolates did not perform their potencies as pathogenic bacteria. Among the 18 non-phytopathogenic bacterial isolates, 14 isolates belong to Gram-positive bacteria and 4 isolates belong to Gram-negative bacteria. Five isolates among Gram positive bacteria were predicted as Bacillus genera. 
Karakterisasi Xilanase dari Bakteri Xilanolitik XJ20 asal Tanah Hutan Taman Nasional Bukit Duabelas Jambi Indonesia INAYAH NOER MAZIDAH; LAKSMI AMBARSARI; ANJA MERYANDINI
Jurnal Sumberdaya Hayati Vol. 2 No. 1 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.1.25-30

Abstract

Xylanase is an extracellular enzymes that can be produced by microbes which capable to degrade xylan into xilo-oligosaccharides (XOS) and xylose. Application of xylanase is still not optimal due to the lack of availability of superior microbial cultures producing xylanase. The purpose of this study was to determine the characteristics of xylanase produced by bacteria isolated from Forest land Bukit Duabelas National Park, Jambi, Indonesia. Xylanase produced by bacterial isolate XJ20 was selected for further enzymatic activity and characteritics assays. Xylanolytic activity was measured by using the 3.5-dinitrosalicylic acid (DNS) assay. High activity of xylanase was recorded at three times of incubation, which were 6 (0.015 U/mL), 14 (0.012 U/mL) and 20 (0.007 U/mL). The first peak and the second peak reached the highest activity at a temperature of 70 °C, however, the first peak reached the highest activity at pH 4.0 and a second peak at pH 7.0. pH 4.0 xylanase were incubated at room temperature and 70 °C has a half time consecutive 9 hours and 6 hours, whereas at pH 7.0, has a half time consecutive 4 hours and 3 hours. Stability of enzyme better at pH 4.0 and at room temperature. 
Bacteria as Greenhouse Gases Reducing Agents from Paddy Plantation MAFRIKHUL MUTTAQIN; MIFTAHUDIN .; IMAN RUSMANA
Jurnal Sumberdaya Hayati Vol. 2 No. 2 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.2.45-51

Abstract

High methane oxidation activity of local isolated methanotrophic bacteria have a potent as methane gases reducing agent while combined with nitrogen fixing bacteria as paddy biofertilizer. The aim of the research was to evaluate the effectiveness of the bacteria as methane gases reducing agent and biofertilizer in paddy plantation. The research was arranged in a completely randomized design consisted of fertilizer types and watering system treatments with four replicates. The research showed that paddy shoot length was not affected by the treatment. On the other hand, both plant freshand dry weight, as well as the number of productive tiller were affected by interaction of fertilizer types and watering system. Fertilizer types affected grain per panicle and methane flux after fertilization. In the end of paddy vegetative stage, bacterial fertilizers were capable to reduce methane emission in different rate. The different result in methane flux was likely due to the interaction between soil local microorganisms and soil chemical component. 
Efektifitas Empat Perangkap Serangga dengan Tiga Jenis Atraktan di Perkebunan Pala (Myristica fragrans Houtt) WINDRA PRIAWANDIPUTRA; AGUS DANA PERMANA
Jurnal Sumberdaya Hayati Vol. 1 No. 2 (2015)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.1.2.54-59

Abstract

The use of traps for insect surveilance on nutmeg (Myristica fragrans Houtt) plantation is one of the earliest steps in this study since the information of nutmeg pest is barely available. In this study, four traps were used, namely Brown-Black Sticky Trap (BBST), Yellow Sticky Trap (YST), Glass Ambrosia Beetle Trap (GABT), and Plastic Pannel Trap (PPT) placed at 1,5 m high on nutmeg plants around Paya Teuk village (South Aceh). Those traps were accompanied by 35%-ethanol, nutmeg oil, and 35%-ethanol mixed with nutmeg oil as attractans. The observation with 4 types of trap with 3 different attractans and water as control have captured 10 orders, 51 families, 124 morphospecies, and 1027 individuals. Five families with the most numerous individuals were Nitidulidae, Tephritidae, Cicadellidae, Formicidae and Scolitidae. Individuals from Scolitidae and Nitidulidae family were mostly captured by BBST with 35% ethanol, and significantly different with the other traps (two way annova, P<0,05). The number of individuals from Tephritidae were mostly caught by YST using nutmeg oil as an attractan (two way Annova, P<0,05). Each type of trap with a spesific attractan effectively catch a different kind of insect family. 
Karakteristik Fisiologis Lumut pada Beberapa Ketinggian di Kawasan Gunung Tangkuban Perahu HIRMAS FUADY PUTRA; DHENOK SEKAR AMBARWATI; NURRIZKI MUBYRASIH; TERSI ALESTI
Jurnal Sumberdaya Hayati Vol. 1 No. 2 (2015)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.1.2.60-63

Abstract

Environmental factors greatly influence the physiological state of moss. Some of the environmental factors that influence the physiological of moss are air temperature, humidity, and light intensity. These three environmental factors will vary at each altitude. This study aims to determine the physiological characteristics of mosses on some altitudes in the area of Mount Tangkuban Perahu (Ratu Crater – Domas Crater track). The method that are used is to measure air temperature, humidity, and light intensity at some altitudes. Subsequently, samples were taken at each altitude, to measured chlorophyll and proline content. Mosses that are used as samples; Campylopus sp., Plagiochila sp., Dicranella sp. The results indicate that the chlorophyll content significantly different at some altitude, with an increasing trend as the decrease altitude. The highest chlorophyll content in Campylopus sp. at altitude of 1,621 meters above sea level, while the lowest chlorophyll content in Plagiochila sp. at altitude of 1,721 meters above sea level. The content of proline did not differ significantly at some altitude, and tend to be more affected by air conditions that correlate with distance of moss habitat with the active crater of Domas, which allegedly can cause physiological stress in the moss. 
Efektivitas Ekstrak Etanol Biji Pinang Terhadap Densitas GLUT4 pada Sel-Sel Otot Rangka Mencit yang Terinduksi Hiperglikemia MULIA SAFRIDA SARI; AHMAD RIDWAN
Jurnal Sumberdaya Hayati Vol. 2 No. 2 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.2.52-58

Abstract

Betel nut (Areca catechu) was proven to have antihyperglycemic activity through increased PI3K pathway in the GLUT4 translocation in cells. However, research on the ethanol extract of betel nut in Indonesia is still limited. The main problem in this study was whether the ethanol extract of betel nut could improve glucose tolerance in a two-hour postprandial period and whether the ethanol extract of betel nut could induce GLUT4 translocation in skeletal muscle cells of mice induced hyperglycemia. Glucose tolerance test results showed that treatment of the ethanol extract of betel nut for 24 days with all doses tested: P50,100,150,200 and 250 mg/kg body weight could improve glucose tolerance in a two-hour postprandial period. The protein electrophoresis of that fasting and one hour postprandial state treatments resulted an increase in GLUT4/βActin density ratio of P250 group compared to negative and positive controls. In this case, GLUT4/βActin density ratio of P100,P200 and P250 were higher than that GLUT4/βActin density ratio of P50 and P150. Increased in GLUT4/βActin density ratio showed enhancement of the GLUT4 in skeletal muscle cells in fasting and one hour postprandial state after treated with ethanol extract of betel nut. 
Kultivasi Bawang Merah Ramah Lingkungan di Wilayah Berpasir Spesifik di Yogyakarta SUTARDI, .; PORWONINSIH, HENI
Jurnal Sumberdaya Hayati Vol. 4 No. 1 (2018)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.4.1.1-6

Abstract

Shallot is one of strategic commodities in Indonesia. Sandy coastal area of Bantul and Kulon Progo districtis the centre of shallot production in Yogyakarta. The results showed that recommendation of fertilizing technology ameliorant (clay 2:1), organik fertilizer 20 ton/ha, Urea 115.7 kg/ha, ZA 250 kg/ha, SP-36 75 kg/ha, and KCl 250 kg/ha. Pest and disease management in integrated pest and disease management 10 Feromon – Exi, Attractant, 10 Light trap, 45 Yellow/white/green trap, biopesticide, limited chemical pesticide, organophosphate pesticide residue contents below with residues below the MRL 

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