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INDONESIA
Biopropal Industri
Published by Kementerian Perindustrian Republik Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering
Arjuna Subject : -
Articles 208 Documents
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Anti-diabetic Activity of Tabat Barito Leafs (Ficus deltoidea, Jack) Extract in Rats Cahyanto, Heru Agus; Supriyatna, Nana
Biopropal Industri Vol 4, No 1 (2013)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Tabat barito (Ficus deltoidea, Jack) leaf is believed could be used to treat diabetes. But more scientific data are needed. The aim of this study is to investigate  antidiabetic activity of  Tabat Barito extract by glucose tolerance method. The treatment was given in three doses (50, 100, and 200 mg/kg BW) and two controls using aquoeus and glibenklamid.  Tabat barito extract was obtained by maceration and made into dry extract with addition of starch. The result showed that chemical compound of the ethanol extract were fenolic and saponin. The extract showed effects in lowering blood sugar levels with glucose tolerance methods at 30 to 60 minutes. Blood glucose levels of mice treated with the extract extract ranged between 132.60 to 258.00 mg/dL , glibenklamide ranged from 130.20 to 144.60 mg/dL, and aqua ranged between 227.60 to 260.20 mg/dL. The percentage decrease in blood sugar levels compared to controls is 32.54%.
Utilization of Cacao Pods Shells For Charcoal Briquettes Suprapti, Suprapti; Ramlah, Sitti
Biopropal Industri Vol 4, No 2 (2013)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Utilization of cacao pod shells waste into charcoal briquettes is a way to find an alternative energy that can be produced within household scale. The aim of the research is to produce charcoal briquettes from cacao pod shells and its characterization. Research was designed with two factors, which is charcoal size (30, 50, and 70 mesh) and the percentage of starch used (3, 5, and 7%). Charcoal briquette produced then tested its physical and chemical properties. In general, charcoal briquettes from cacao pod shells with a mesh of 30 and 7% starch gives the best results. This charcoal briquette have a moisture content of 6.52%, ash content 16.73%, density 0.96 g/cm3; levels of volatile matter 20.18%, bonded carbon content 56.58%, and calorific value 4163.11 cal./g. Most of the parameters have not been meet the quality standards for charcoal briquettes of the SNI, Japan, England, nor American.
Fermentative Extraction of Coconut Oil to Maintain A Quality of Medium Chain Fatty Acid Salahudin, Farid; Supriyatna, Nana
Biopropal Industri Vol 5, No 1 (2014)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Coconut oil is healthy vegetable oil because it contains Medium Chain Fatty Acid (MCFA). The used of bleaching agent and excessive heating in coconut oil process will produce low quality oil (rancid). Therefore, it is necessary to processing that does not use chemicals and excessive heating such as fermentation using microbe and enzyme. The aim of this study was to find out the effect of bromelin enzyme concentration and Saccharomyces cereviceae fermentation to MCFA content in coconut oil. This research was done by adding the enzyme bromelain at 0, 50, 100, 200 and 400 ppm and S. cereviceae inoculated in coconut milk. The resulting oil is then tested the water content, acid number and fatty acid content. The result showed that fermentation with 200 ppm bromeline enzyme and S. cereviceae inoculation can produce the best quality coconut oil containing MCFA that meet the APCC standard.
Optimization of Incubation Time on Cellulase Enzyme Production Using Aspergillus niger Under Solid State Fermentation Ariyani, Sukma Budi; Asmawit, Asmawit; Utomo, Pramono Putro
Biopropal Industri Vol 5, No 2 (2014)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Cellulase is an important enzyme for industry which can be obtained from cheap tropical agrowastes such as durian peel. West Kalimantan, in particular Pontianak city produces tons of durian peel waste during harvest season each year. This study used durian peel waste as substrates and A. niger to produce cellulase, The aim of this study is to determine the optimum incubation time in producing cellulase under solid state fermentation. Different incubation time (48, 72, 96, 120 and 144 hours) were applied for the production of cellulase. The optimum cellulase production was 120 hours of incubation, giving  protein content of 0.3960% and enzyme activity of 1,069.01 ppm, respectively.
Patchouli Oil Production for Fixative Aromatheraphy: A Case Study Design of Condenser for Steam Distillation Agustian, Egi; Sulaswatty, Anny
Biopropal Industri Vol 6, No 1 (2015)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Patchouli oil is an important material for aromatherapy industries and made from patchouli leaves by steam distillation. The important parts of steam distillation is condenser. The problem is process of farmers patchouli based on experience not used chemical process of steam distillation cause not effective and efficient. The study was designed of condensers on steam distillation for patchouli leaves. Specification of condenser type is shell and tube, stainless steel material and water as coolant. Tubing sized is ¼ inch with 500 mm of length and 24 pieces. Shell measuring 2.5 inch and 600 mm of length. The performance of condenser gave the efficiency at 94.51%. The process condition of ratio stems to leaves is 5:6 and 4.5 hours was considered ideal for steam distillation. The used of energy is lowest cause the efficiency condenser and yield will be higher. Patchouli oil yield of 2.39% and patchouli alcohol content is 35.65%.
The Formulations of Scrub Cream from Non Fermentation Cocoa Powder and The Effects on Skin Yumas, Medan; Ramlah, Sitti; Mamang, Mamang
Biopropal Industri Vol 6, No 2 (2015)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

The content of non fermented cocoa powder is phenolic compounds of polyphenols group which protecting skin from ultra violet radiation (UVB). This research aims to determine the concentration of non fermented cocoa powder that capable to protect the skin from UVB. This study used a complete randomized design with a treatment on the concentration of non fermented cocoa powder as the active ingredient of scrub cream. Scrub cream was made from non fermented cocoa powder in a variable concentration 2.5; 3.5; 4.5; 5.5% (w/w) with three replicates. The results showed that the viscosity were 36, 42, 49 and 70 dps, respectively. The four scrub cream have pH value above 5 which fulfilled the standard of SNI 01-3524-1994 and uncontained of Pb and Hg. The most panelists was preferred scrub cream with the concentration of non-fermented cocoa powder 3.5%, it gave moisture and smooth effect also lasted minimum for 180 minutes.
The Effect of Cocoa Beans Fermentation on Processed Chocolate In West Kalimantan H, Jhon David; P, Tommy
Biopropal Industri Vol 2, No 1 (2011)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Research studies of fermentation effects on processed chocolate have been made. The purpose of this study was to see the effect of different levels of fermentation of cocoa preparations (pasta, fats and powder) to the chemical quality and flavor. Harvested Cocoa beans will be treated fermentation. The treatment used was the time of fermentation namely: (A) Non fermentation, (B) Imperfect fermentation (4 days) and (C) Perfect fermentation (5 days). The parameters observed was: (a) chemical quality namely water content, fat content, pH, (b) organoleptic tests namely color, aroma, taste bitter (bitterness), texture. The results showed that fermentation of cocoa beans have real impact on levels of fat, water content and pH. Panelists gave the highest rankings for quality of organoleptic attributes of pasta, fat and chocolate powder from perfectly fermented beans. Processing of cocoa beans into refined products had levels of depreciation for each stage. 100 kg dried cocoa beans could produce 79.0 kilograms of chocolate paste, 48.7 kg of cocoa powder, and 26.5 kg of fat. Fermentation for 5 days is the best solution to improve the quality of processed chocolate.
Determination of Pseudomonas Bacteria Species and Analysis of Phylogenetic Tree using Bioinformatics Method Suyono, Yoyon
Biopropal Industri Vol 1, No 2 (2010)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Bioinformatics is the application of information technology in the field of biology by organizing and analyzing genetic data from a database of DNA, RNA and proteins into meaningful biological information. One role of bioinformatics is to identify the unknown bacteria and analysis of phylogenetic relationships based on genetic information is relatively easy, quick and cheap.The research aims to determine species and analyzed the phylogenetic tree bacteria of the genus Pseudomonas in Bioinformatics.  Research conducted through the stages of isolation, testing and continuing 16S rRNA bioinformatics studies include, for studies using the Basic Local Alignment Similarity Search Tool (BLAST) from NCBI, Multiple Sequence Alignment (MSA) using Clustal W from the EMBL-EBI and visualization in a phylogenic tree using the Tree View software. Isolation is obtained 2 (two) isolates the code 251 and 252. Testing 16S rRNA sequences of nucleotides in Fasta format. BLAST analysis in GenBank database of identity obtained respectively 99%, E-value 0.0 and the value of bits score above 50, Pseudomonas aeruginosa strains MZA-85 and Pseudomonas sp. J16. Multiple sequence alignment (MSA) and visualization of tree phylogenetic homologous isolates of code 251 and has a kinship with the species Pseudomonas aeruginosa Isolate 29 and isolate the code 252 with a species of Pseudomonas sp. J16 with a score of 99% and 100%. Isolates obtained by including the genus Pseudomonas species, Pseudomonas aeruginosa and Pseudomonas sp that has a nitrogen-fixing ability and resistance to the herbicide.
Search Region of Origin Honey Bee A. mellifera in Indonesia Region Using Mitochondrial DNA intergenic cox1/cox2 Hidayat, Mohamad Rusdi
Biopropal Industri Vol 2, No 2 (2011)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Apis mellifera is a favourite honey bee for the beekeepers throughout many countries. This species comprise of 24 subspecies. Based on phylogeography and morphometric evidences, these subspecies have been grouped into four lineage; namely the African (A), Western and Northern Europe (M), Southeastern Europe (C), and Near Eastern (O). Apis mellifera have been imported to Indonesia since 1972, and mostly from Australia. However, until recently there are no data about the A. mellifera subspecies and the origin. Therefore the objective of this research is to determine the lineage of A. mellifera in Indonesia based on mtDNA intergenic region between cox1/cox2 genes. In this region there are two DNA fragments, P and Q fragnant, that can be used to determine the A. mellifera lineage. The methodology used consist of samples collection, DNA isolation, DNA amplification, DNA restriction using DraI enzyme, DNA sequencing, and DNA alignment using Clustal X and MEGA spftwares. DNA fragment amplified by using E2 and H1 primer revealed a 863 bp. Digestion of the region with the DraI restriction enzyme revealed one haplotype, which consist of five DNA fragments. Based on DNA sequences and DNA alignment, A. mellifera in Indonesia was homologue with the C lineage. Its subspecies is A. m. ligustica that lived natively in Italy, they were imported to Indonesia from Australia
Adsorption Capacity of Mercury Using Sargassum crassifolium Activated Adsorbent Silalahi, Imelda H; Zahara, Titin Anita; Tampubolon, Henry Martua
Biopropal Industri Vol 3, No 1 (2012)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

This study aims to determine the maximum adsorption capacity of Hg using activated Sargassum crassifolium adsorbent. Sargassum crassifolium was activated by protonation using sulfuric acid (H2SO4) 1 M followed by heating for 24 hours at 100OC. Optimum conditions of adsorption was discovered through various of pH and contact time to the Hg(II) concentration decrease. The analysis showed that the optimum pH was 7 and contact time was 60 minutes. Finally the maximum adsorption capacity of Hg(II) using activated Sargassum crassifolium adsorbent was 2,90 mmol/g determined by Langmuir Isotherm equation.

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