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Y. Andi Trisyono
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INDONESIA
Jurnal Perlindungan Tanaman Indonesia
Published by Universitas Gadjah Mada
ISSN : 14101637     EISSN : 25484788     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 509 Documents
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Correlation between Epidermis Thickness, Leaf Trichome Length and Density with the Whitefly Bemisia tabaci Population on Five Local Soybean Cultivars Muhamad Lutfi; Purnama Hidayat; Nina Maryana
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.34498

Abstract

Soybeans compsumsion in Indonesia is about 2.7 million ton, while the national production is only about 0.7 million ton per year. There are some problems in the soybean production in Indonesia, including pests and diseases. Whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is one of the important pests in soybeans causes a significant loss of yields. Developing soybean cultivars resistant to whitefly is one of method to reduce the production loss. This study aimed to determine the relationship between the physical characteristics of lower surface leaves and B. tabaci population on five local soybean cultivars. The experiments were conducted in the IPB greenhouse by Completely Randomized Design (RCD) using five soybean cultivars (Anjasmoro, Dena-1, Detam-3, Devon-1, and Wilis) with 3 replicates. Three plants of each cultivar were planted in a polybag, put under a cage, and after one week then infested with 5 female whiteflies. The parameters observed in this study were leaf epidermal thickness; density and length of trichome on lower leaf surface; and the number of egg, nymph, and adult of the whitefly. Observations were carried out every week, from 1−7 weeks after the plant was infested with the whitefly. The population of whitefly was affected by trichome density and length, as well as by the epidermal thickness of the lower leaf surface. The infestation of B. tabaci was higher in the soybean cultivars with denser and longer leaf trichomes, and thinner epidermal of the lower leaf surface. This result of this research suggests that it is necessary to include the characteristics of leaf trichome and epidermal thickness in the breeding program to produce soybean cultivars that are superior in productivity and resistant to whitefly.
The Effectiveness of Several Plant Extracts to Induce Rice Plant Resistance against Bacterial Leaf Blight - (Xanthomonas oryzae pv. oryzae) Fitri Widiantini; Avissa Ayuningdiyas; Endah Yulia; Tarkus Suganda
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.34912

Abstract

Resistant plants are one of the disease control techniques that considered to be effective. Resistant plants can be produced in various ways including the application of plant extracts. The aim of this study was to examine the ability of several plant extracts to increase the resistance of rice plants to bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo). A total of 13 plants were extracted and applied in two methods, which were seed treatment and seedling treatment which sprayed on two-week old rice seedlings. Xoo bacteria were inoculated on rice plants two weeks after planting. The observations on the intensity of BLB disease infection showed that water hyacinth extract (Eichhornia crassippes), spiny amaranth (Amaranthus spinosus) and jasmine leaves (Jasminum grandiflorum) can suppress the development of BLB disease in both application methods. The application of plant extracts as inducing agents needs to be repeated to maintain the activated plant defense mechanism.
Selection and Inheritance of Tomato Resistance against Ralstonia solanacearum Isna Maulida; Rudi Hari Murti; Triwidodo Arwiyanto
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.35464

Abstract

Ralstonia solanacearum is a plant pathogen causes wilting which is a major obstacle in the cultivation of tomato plants. In plant breeding, knowledge of the source of resistance genes and inheritance patterns is important in the development of bacterial wilt resistant varieties. This study aimed to obtain bacterial wilt resistant lines and to find out the inheritance pattern of tomato resistance to bacterial wilt. Selection of resistant plant involved the selected breeding lines from irradiation and crossing collections of the Genetic Laboratory, Faculty of Agriculture, Universitas Gadjah Mada. Introduced lines of H-7996 and F1 Permata and Timoti were used as a control. H-7996 as resistant parents and GM2 as susceptible parents, and their offspring include F1 GM2 x H-7996, F1 reciprocal, F2, Back Cross 1 (F1 x GM2), and Back Cross 2 (F1 x H-7996) used in testing inheritance patterns. Inoculation was carried out 1 week after planting by pouring 100 ml of water suspension of R. solanacarum (108  cfu/ml) on the roots. Completely Randomized Design (CRD) was used in this experiment. The scoring observation was carried out every week for one month. This study showed that Permata as a control was the most resistant, while Timoti and H-7996 were medium resistant. The CLN, G6, G8, and G7 lines were susceptible medium, yet only G8 and G7 with the smallest percentage of disease intensity and not significantly different than Timoti. The resistance gene to bacterial wilt on H-7996 was controlled by genes in the cell nucleus with additive-dominant gene action. Resistance to bacteria has a moderate level of heritability.
Analysis of Volatile Compound at Different Age of Corn Crops Used as Bemisia tabaci Repellent Retno Wikan Tyasningsiwi; Witjaksono Witjaksono; Siwi Indarti
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.35954

Abstract

Bemisia tabaci is one of the red chili pests that plays a role as a Begomovirus vector. This vector can be controlled through Integrated Pest Management (IPM) practices. One of the potential practices is by utilizing corn as a barrier crop to prevent the vector from attacking the main crop. The aim of this research was to examine the repellence activity of the volatile compound obtained from various ages of corn crop against B. tabaci. The volatile compound was collected from the corns at 4 week after planting (WAP), 6 WAP, 8 WAP, 10 WAP, and 12 WAP. Volatile compound capture device was designed with two solvents, i.e. hexane and ethanol, then the volatile compound analyzed by Gas Chromatography Mass Spectrometry (GC-MS). B. tabaci repellency against volatile compound was examined using Y-tube olfactometer. Each treatment was employed with 5 replications. Data were analyzed using ANOVA with 95% and further analyzed by Least Significant Different (LSD). The parameter observed was the total of B. tabaci avoided the volatile compound tested until half of the control olfactometer arm. The results showed that all ages of the corn produced repellent compound and the most optimal were corn aged 12 WAP with repellency rate of 83.72%. GC-MS analysis identified the volatile compound were citronella, limonene, β-phellandrene, β-caryophyllene, 1.8 cineole, farnesol, caryophyllene, and patchouli alcohol.
Detection and Analysis of Protein Profile on Rice Infected by Stunting Virus with Different Severity on Ciherang and Situ Bagendit Varieties Selvi Helina; Sri Sulandari; Sedyo Hartono; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.36549

Abstract

Rice stunt virus is one of the limiting factors in the decline of rice production in Indonesia. The virus consists of rice grassy stunt virus (RGSV) and rice ragged stunt virus (RRSV) that is transmitted by brown planthopper (WBC) in a persistent propagative manner. This study aimed to determine the presence of rice stunt virus in Bantul, Yogyakarta through fast detection using RT-PCR. It also aimed to learn the pattern of total protein profile of healthy and infected rice plants by the virus on different severity level in the field. The results showed that rice varieties of Ciherang and Situ Bagendit in Bantul were infected with RGSV and RRSV. They were classified as mild, moderate, severe, and failure in severity level. Homology analysis using BioEdit showed that the nucleotide sequence of RGSV in Bantul isolate had the highest percentage of nucleic acids similarity with Klaten isolate (98.1%). Meanwhile, RRSV of Bantul isolate had the highest percentage of nucleic acids similarity to Philipines isolate (99.5%). Analysis of protein profiles using SDS-PAGE showed a pattern of protein profiles formed on rice infected with the virus at different severity levels which was not found in healthy rice. These proteins presumably were nonstructural p5 and nucleocapsid protein (NCP) of RGSV with a molecular weight of ~22 and 34-35 kDa; and viral spike protein and protein capsid (S8) of RRSV with MW ~39 and ~43 kDa.
The Vertical Distribution of Helopeltis bradyi and Oxyopes javanus on Tea Novira Maya Sari; Arman Wijonarko; Fransiscus Xaverius Wagiman
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.38118

Abstract

Helopeltis bradyi is the main pest of tea plants. Ecological characteristics of this pest are important to be understood to support the development of their management and control measures. This study aimed to determine the coexistence and vertical distribution pattern of H. bradyi and its predator, Oxyopes javanus, on tea plant parts. The study was conducted at the PT Pagilaran tea plantation in Central Java, in the 2018 rainy season. Population observations were carried out in situ on 20 infested sample-trees taken randomly, for 10 consecutive days, in the morning, at noon, and in the afternoon. Vertical distribution patterns were determined based on Poisson dispersion index (DI), negative binomial, and Green index (GI). The results showed that the O. javanus spider was found preying on H. bradyi. Coexistence between this pest and predator in the same part of the plant, the pest, and the predator, occurred in the morning were 50.0, 42.8, and 7.2%; at noon were 58.3, 41.7, and 0%; and at the afternoon were 66.7, 33.3, and 0%, respectively. The parts of the plant for the coexistence are pekoe leaves; the 1st, 2nd, 3rd, and 4th of young leaves; and 1st of older leaves. The pattern of vertical distribution in the morning, at noon and in the afternoon for H. bradyi was the weak clump, while for O. javanus was uniform. The ratio of predator: prey in the morning, at noon and in the afternoon was 1:10.7, 1:16.7, and 1:10.0, respectively.
Detection and Identification of Banana-associated Phytoplasma Using Nested-PCR Method Saurma Mona Astrid Sibarani; Tri Joko; Siti Subandiyah
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.38320

Abstract

Phytoplasma is known to be associated with plant diseases in about 300 plant species from various families. Information on the presence of phytoplasma in bananas as one of the pathogens that can cause disease in bananas in Indonesia has never been reported. This research was conducted with the aim to detect the presence of banana phytoplasma by the nested-PCR method and to identify phytoplasma obtained based on the sequence analysis of the 16S rRNA gene. Standard PCR was carried out using P1/P7 primary pairs, followed by nested-PCR using a pair of R16F2n/R16R2m23SR primers separately that could amplify the target 16S rRNA genes in a row at 1600 bp. BLAST analysis shows that the results of phylogenetic analysis of banana phytoplasmic nucleotide cv. manggala from Tasikmalaya and cv. Raja nangka from Banjar has a genetic relationship that is closer to lethal wilt oil palm Phytoplasma (Candidatus Phytoplasma asteris). This phytoplasma belongs to the 16SrI-B group (aster yellows).
The Effect of Silica (SiO2) to the Severity of Yellow Leaf Curl Disease on Chili Pepper Dewa Gede Wiryangga Selangga; Sri Hendrastuti Hidayat; Anas Dinurrohman Susila; Suryo Wiyono
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.38951

Abstract

Yellow leaf curl disease of chili pepper caused by Pepper yellow leaf curl virus (PYLCV) has been reported as an important disease in Java and Bali. Disease severity reached 80−100% and it may cause significant yield losses. In order to reduce the negative impact of synthetic insecticides, silica application was evaluated for its potency to suppress the disease. A greenhouse experiment was conducted using randomly block design with 2 factors: PYLCV isolate (Java and Bali) and silica (SiO2) treatment (with and without). Parameters observed were disease symptoms, incubation period, disease incidence and severity, and total silica level. The symptoms of virus infection in Pelita 8 and Seret cultivars were yellow mosaic, leaf curl, green mosaic, dwarf, and cupping upward or downward. A Specific DNA fragment of 912 bp was successfully amplified from 4 samples. Four sequences were obtained and further analysis showed their highest homology, i.e. 96% and 97% with Pepper yellow leaf curl Indonesia virus-Java (PYLCIV-Java) (JX416180) and PYLCIV-KrthAl (LC381274), respectively. Infection by different virus isolates did not affect disease severity significantly. The application of silica was able to delay symptom development and to suppress the severity of the disease in the range of 16.67−30.33%. Silica application on the soil increased the total content of silica in the plants. However, a further experiment is required to understand the mode of action of silica in inducing plant resistance to the pathogen.
Antagonism Mechanism of Epiphytic Yeast against Anthracnose Pathogen (Colletotrichum acutatum) on Chilli Sri Hartati; Suryo Wiyono; Sri Hendrastuti Hidayat; Meity Suradji Sinaga
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.40951

Abstract

Epiphytic yeasts have the potency as antagonistic agents against various pathogens of post-harvest products. Anthracnose is a major disease of chilli that causes high economic loss. This research was objected to study the antagonism mechanism of epiphytic yeast isolates that have the antagonistic potency against anthracnose pathogen on chilli (Colletotrichum acutatum). Twenty-two isolates of epiphytic yeasts, isolated from chilli leaves and fruit, were tested. The characterization of the antagonism was carried out by antibiosis, anti-fungus volatile production, and chitinolytic activity tests. The results showed that all tested isolates did not have antibiosis mechanism against C. acutatum. All isolates produced volatile compounds which inhibited the colony growth of C. acutatum. Four isolates showed high relative inhibition rate, i.e. isolates B32DEP (35.68%), B30DEP (37.52%), B23DEP (38.52%), and B29DEP (45.42%). Fourteen isolates showed chitinolytic activities. Three of them had high chitinolytic activities, i.e. B12DEP, B2DEP, and G237DEP.
Fungal Pathogens Associated with Vascular Streak Dieback (VSD) Disease on Cacao in Special Region of Yogyakarta Province Hendra Hendra; Arif Wibowo; Suryanti Suryanti
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.41512

Abstract

Cacao is one of the pre-eminent crops plantation with high economic value. Indonesia's cacao beans production is the third largest in the world after Ivory Coast and Ghana. Vascular Streak Dieback (VSD) is one of the important diseases of cacao which caused a decreased yield either in quantity or quality. The disease is caused by the basidiomycete fungus Ceratobasidium theobromae (syn. Oncobasidium theobromae). Spores are carried by wind to spread, infect young leaves and penetrate through natural openings and colonize xylem vessel which could inhibit the transportation system in the plant tissue. There are several fungal pathogens associated with VSD diseases on cacao. This research aimed to study the fungi associate with VSD diseases on cacao in Special Region of Yogyakarta Province. Survey and sampling were conducted in cacao plantations in regencies of Gunungkidul, Kulon Progo, Bantul and Sleman. The severity of VSD disease in the regencies of Gunungkidul and Kulon Progo were high, while in the regencies of Sleman and Bantul were moderate. Eighty eight fungal isolates were isolated from infected petiole and stem. The in vitro pathogenicity test screened 32 fungal isolates causing necrotic and chlorotic symptoms on young healthy cacao leaves with and without wounding. The first symptoms appeared at 8-12 days after inoculation and fungal mycelium could grow at 1−3 days after inoculation. Those isolates collected showed a high diversity of colony morphology. Lasiodiplodia sp., Fusarium sp., Colletotrichum sp., and Pestalotiopsis sp. had been identified based on conidial morphology.

Page 38 of 51 | Total Record : 509

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