Claim Missing Document
Check
Articles

Found 17 Documents
Search

Potensi Metabolit Sekunder Asal Bakteri Endofit dalam Menekan Pertumbuhan Miselium Ganoderma boninense Fitri Widiantini; Ceppy Nasahi; Endah Yulia; Serly Noviyawati
Jurnal Fitopatologi Indonesia Vol. 14 No. 3 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (598.628 KB) | DOI: 10.14692/jfi.14.3.104

Abstract

Potency of Endophytic Bacterial Secondary Metabolite to Inhibit Mycelium Growth of Ganoderma boninense Endophytic bacteria have a close relationship with their host plants. The bacteria are living inside their host without causing any symptom. Endophytic bacteria are well known for their ability to produce compounds that can be used to protect plants from deleterious microorganisms. The research was conducted to determine the ability of endophytic bacteria in producing antifungal compounds that are able to suppress the growth of Ganoderma boninense, a causal agent of basal stem rot disease of oil palm. Previously isolated endophytic bacteria were grown on international streptomyces project 2 (ISP2) liquid medium and incubated at rotary evaporator at 120 rpm for 10 days. The supernatant was separated from bacterial cells and tested against G. boninense using well diffusion agar method.  The results showed that endophytic bacteria isolate of BEK6, BEK7, BEK9 and BEK10 were able to produce antifungal compound against G. boninense. Microscopic observation demonstrated G. boninense mycelia alteration following secondary metabolite application. 
Ekstrak Tanaman Binahong sebagai Pengendali Penyakit Hawar Pelepah Daun Padi Endah Yulia; Fitri Widiantini
Jurnal Fitopatologi Indonesia Vol 14 No 4 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1281.382 KB) | DOI: 10.14692/jfi.14.4.138

Abstract

Binahong Extract for Controlling Sheath Blight Disease of Rice PaddySheath blight disease, caused by Rhizoctonia solani, is an important disease that can affect rice production. The disease is commonly controlled using synthetic fungicides. This study was aimed to test the potential of binahong plant (Anredera cordifolia) as botanical fungicide to inhibit the growth of R. solani and the development of blight disease on rice seedlings. The research was conducted in 2 stages, i.e. in vitro and rice seedling testing. In vitro test was carried out on PDA medium containing different concentrations of binahong leaf extract (0.125%, 0.25%, 0.5%, 1%, and 2%). Rice seedling testing was completed by soaking rice seeds in five concentrations of binahong leaf extract followed by inoculation of R. solani.  The experiments were arranged in a completely randomized design. The variables observed consist of inhibitory ability of binahong leaf extract, colony morphology of R. solani, and disease incidence. Binahong leaf extract inhibited 35.2% growth of R. solani colonies. Treatment of binahong leaf extract had some effects on mycelium of R. solani; macroscopically the mycelium was shorter, thinner and congregated, whereas microscopically it was smaller, elongated and tended to be colourless compared to control. The treatment of 2% binahong leaf extract on rice seeds decreased the incidence of seedling blight disease with the effectiveness reached 100%. Thus, binahong leaf extract has the potential to suppress sheath blight disease caused by R. solani.
Variasi Morfometri dan Patogenisitas Peronosclerospora spp. Penyebab Penyakit Bulai Jagung di Pulau Jawa, Indonesia Satriyo Restu Adhi; Fitri Widiantini; Endah Yulia
Jurnal Fitopatologi Indonesia Vol 17 No 5 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.5.173-182

Abstract

Morphometric and Pathogenecity Variation of Peronosclerospora spp. The Causal Agent of Maize Downy Mildew in Java Island, Indonesia Downy mildew disease in maize caused by Peronosclerospora spp. has been reported to cause yield loss in several production centers in Java. This study aimed to determine the morphometric characteristics and pathogenicity of Peronosclerospora spp.. Ten strains of Peronosclerospora were collected from maize production center in Blitar (BLT), Kediri (KDR), Kediri 2 (KDR2), Klaten (KLT), Cianjur (CJR), Garut (GRT), Jatinangor Sumedang (JTN), Rancakalong Sumedang (RCG), Indramayu (IMY), and Sukabumi (SKB). Morphometric variation was determined using microscope by observing shape of conidium; measuring cell wall thickness, length of conidiophores, dimensions of conidia; and counting the number of branches. Differences in morphology and pathogenicity between strains was evidenced. Dendogram analysis based on morphometric characters differentiated strains of Peronosclerospora into 2 main clusters. One strain, KDR2 is in the same group and identical with reference strain P. philippinensis; while the others are in the same group and identical with reference strain P. maydis. Pathogenicity test showed that IMY strain caused the lowest disease incidence (8.33%) and KLT strain caused the highest disease incidence (47.92%).
Assessment of Sarocladium oryzae Growth Inhibition, the Causal Agent of Rice Sheath Rot Disease, Using Methanol Extract of Binahong Leaves Endah Yulia; Silviya Rahma Dhiya Syafira; Fitri Widiantini; Wawan Kurniawan
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.6 KB) | DOI: 10.24198/cs.v2i1.22301

Abstract

Sheath rot disease caused by Sarocladium oryzae is an important disease on rice. The pathogen that infects uppermost leaf sheath enclosing to the young panicles can cause yield losses up to 85%. Though the disease is commonly controlled using synthetic fungicides, the use of natural products that safer to the environment have been enormously explored. The research aimed to test growth inhibition of S. oryzae colony using methanol leaf extract of binahong (Anredera cordifolia). The research was carried out from June until December 2017 at the Laboratory of Biotechnology Plant Protection, Department of Plant Pest and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The method used was Randomized Complete Design consisted of six treatments and four replications. The treatments were five concentrations of binahong leaf extract of 0.125%, 0.25%, 0.5%, 1%, 2% and one control treatment. The result showed that binahong leaf extract did not inhibit the growth of S. oryzae. The highest inhibition percentage of 4.55% at 0.25% concentration was considered to be a low inhibition category. Although the colony growth of S. oryzae was not significantly inhibited, the extract caused hyphal abnormalities. This actually shows the antifungal potency of the binahong leaf extract that needed further examination with more adequate methods.Keywords : Anredera cordifolia, botanical pesticide, rice sheath rot
Potency of Rhodotorula minuta as Plant Growth Promoter on Red Chili Plant Infected by Colletotrichum acutatum Sri Hartati; Linda Tarina; Endah Yulia; Luciana Djaya
CROPSAVER Vol 2, No 2 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (465.413 KB) | DOI: 10.24198/cropsaver.v2i2.22542

Abstract

Some yeast species were reported to have the ability to promote plant growth. Rhodotorula minuta is a yeast species that has the potency as biocontrol agent. This research was objected to evaluate the potency of R. minuta in promoting the growth of red chilli infected by Colletotrichum acutatum. The experiment was carried out at the Laboratory of Plant Protection Biotechnology and Green House of Ciparanje Experiment Station, Faculty of Agriculture, Universitas Padjadjaran. The experiment was arranged in the randomized completely block design with 9 treatments and 5 replications. The treatments were infestation of R. minuta and inoculation of C. acutatum on 5, 7, and 10 days after replanting, and a positive control with no R. minuta infestation but inoculated with the pathogen and a negative control without R. minuta infestation nor pathogen inoculation. Results of the experiment showed that the effectiveness of the treatments with R. minuta on the increase of the chilli plant height was 3.78-17.90%, leaf number was 4.09-15.19%, and dry weight of the biomass was 0.14-0.58%. This results indicated that treatments with R. minuta were able to promote the growth of chilli plants.
The Effect of Neem, Citronella, Castor Oil, and Their Mixtures against Crocidolomia pavonana F. (Lepidoptera: Crambidae) Leni Maryani; Danar Dono; Endah Yulia
CROPSAVER Vol 2, No 2 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (573.009 KB) | DOI: 10.24198/cropsaver.v2i2.24063

Abstract

Cabbage-heart caterpillar, Crocidolomia pavonana F. (Lepidoptera: Crambidae), is one of the important pests in Brassicaceae family. Alternative control for cabbage-heart caterpillar that can be used is insecticide made from plant extracts. Neem, cirtonella and jatropha oil are reported to have an insecticidal properties. This study aimed to determine the effect of singgle or combination of neem, citronella, and castor oil to against C. pavonana larvae and to obtain the most effective ratio and concentration of oil combinations. The experimental method used in this research was Randomized Block Design with 41 treatments, i.e. control, single neem, citronella, castor oil and its combination each tested at concentration of 1%; 0,5%; 0,25%; and 0,1%. Each treatment was replicated 3 times. The results of this experiment showed that single oil or combination of neem, citronella and castor oil at consentration of 1% and 0,5% caused 100% larvae mortality, 1:3:2 ratio at 1% concentration was able to reduce food consumption, 3:2:1 ratio at 0,25% concentration was inhibit larvae development and reduce the weight of pupae, and 3:1:2 ratio at 0,5% concentration can reduce the weight of larvae.
Effectiveness of Modified Traps for Protection Against Fruit Flies on Mango Agus Susanto; Sudarjat Sudarjat; Endah Yulia; Agus Dana Permana; Aries Gunawan; Dwi Harya Yudistira
Jurnal Biodjati Vol 5, No 1 (2020): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i1.7926

Abstract

Mango is one of the primary fruit export commodities in Indonesia. The productivity of mango can be increased by controlling the pest population, including fruit flies Bactrocera spp. Various efforts to control the population of fruit flies have been carried out, either by implementing traditional methods or by using synthetic pesticides. However, synthetic pesticides can cause pollution, and therefore we need to control the fruit flies’ population by using the most effective fruit fly trap mode. The study was conducted in the mango yield (monoculture) of Department of Agriculture in Majalengka. The traps in this study were modified Steiner traps with funnels, modified Steiner traps without funnels, bottle traps with funnels, bottle traps without funnels, and modified gypsy moth traps. Each trap was fed with 0.2 ml of Methyl eugenol dripped on a cotton roll and hung in the trap. The results showed that the number of fruit flies caught in each trap every week is significantly different. The highest number of fruit flies caught in each trap every week was achieved by bottle traps without funnels that caught 135 flies, while the lowest number of fruit flies caught was recorded by the gypsy moth traps with 16 flies. The transparent color of the bottles and a large number of holes effectively lured the fruit flies into the bottle traps without funnels.
MANAJEMEN APLIKASI PESTISIDA TEPAT DAN BIJAKSANA PADA KELOMPOK TANI PADI DAN SAYURAN DI SPLPP ARJASARI Endah Yulia; Fitri Widiantini; Agus Susanto
Kumawula: Jurnal Pengabdian Kepada Masyarakat Vol 3, No 2 (2020): Kumawula: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kumawula.v3i2.27459

Abstract

SPLPP Arjasari di Desa Arjasari, Kabupaten Bandung merupakan tempat pelatihan dan pengembangan pertanian yang dimiliki Universitas Padjadjaran. Kelompok tani di bawah arahan SPLPP Arjasari banyak menanam komoditas tanaman pangan dan sayuran seperti padi, jagung, kedelai, cabai, tomat, dan buncis. Namun demikian, keberadaan organisme pengganggu tanaman (OPT) menjadi salah satu masalah utama bagi petani dimana pengendaliannya masih bergantung pada penggunaan pestisida kimia. Permasalahannya adalah sebagian besar petani masih melakukan aplikasi pestisida sintetik secara rutin serta sering menggunakan dosis yang tidak sesuai anjuran. Hal tersebut telah menimbulkan beberapa kerugian seperti mahalnya biaya pengendalian OPT, tidak efektifnya pestisida yang digunakan dan kemungkinan timbulnya efek lain seperti resistensi, residu, serta pencemaran lingkungan. Tujuan dari kegiatan ini adalah untuk meningkatkan pemahaman, keahlian dan keamanan petani dalam pengaplikasian pestisida di Kelompok Tani Arjasari. Metode yang digunakan dalam kegiatan pengabdian ini adalah metode pendekatan kelompok melalui penyuluhan, pelatihan partisipatif, dan studi lapangan dengan mengintegrasikan antara teori dan praktek. Pelaksanaan dilakukan dalam bentuk ceramah, instruksional, diskusi, dan praktek lapang. Petani diajarkan teknik pengendalian OPT secara tepat dan bijaksana, ekologi dan biologi OPT, teknik aplikasi pestisida dan kalibrasi alat, bahaya pestisida baik bagi jasad non target, bagi dirinya dan orang lain, serta gejala akibat paparan pestisida dan cara pertolongan pertamanya. Melalui kegiatan ini petani dapat menambah pengetahuan dan pemahaman di dalam upaya peningkatan keberhasilan pengendalian OPT yang tepat dan bijaksana di SPLPP Arjasari, sehingga akan pula meningkatkan kualitas dan kuantitas produksi pertanian mereka.SPLPP Arjasari in Arjasari Village, Bandung Regency is a place for training and agricultural development owned by Universitas Padjadjaran. Farmer groups under the direction of SPLPP Arjasari are grown quite diverse food crops and vegetables such as rice, corn, soybeans, chillies, tomatoes, and beans. However, the presence of plant pests dan diseases becomes one of the main problems for farmers. The most common control method using by farmers is the use of chemical pesticides. The problem is that most farmers still routinely apply synthetic pesticides and often use doses that are not as recommended. This has caused several disadvantages such as the high cost of pest control, the ineffectiveness of pesticides used and the possibility of other effects such as resistance, residue, and environmental pollution. The purpose of this activity was to increase the awareness, expertise and safety of farmers in the application of pesticides in the Arjasari Farmers Group. The method used in this community service was the group approach method through counseling, participatory training, and field studies by integrating theory and practice. Activity implementation was carried out in the form of lectures, instructional, discussion, and field practice. Farmers were taught proper and wise pest control techniques, ecology and biology of pests and pathogens, pesticide application techniques and tool calibration, the dangers of pesticides both for non-target organisms, for themselves and others, as well as symptoms due to pesticide exposure and how to perform first aids. Through this activity, farmer knowledge and understanding in increasing the success of appropriate and wise pest control in the Arjasari SPLPP will be increased, so that it will also improve the quality and quantity of their agricultural production. 
Pengaruh Ekstrak Kasar Umbi Udara Binahong (Anredera cordifolia (Ten.) Steenis) terhadap Penghambatan Koloni dan Kejadian Penyakit Akibat Alternaria solani pada Bibit Tomat Endah Yulia; Rumenda Tamariska Bangun; Tohidin Tohidin; Hersanti Hersanti
Agrikultura Vol 32, No 3 (2021): Desember, 2021
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v32i3.34683

Abstract

Alternaria solani merupakan patogen penyebab penyakit bercak cokelat pada tanaman tomat. A. solani mampu menyerang hampir seluruh bagian tanaman yaitu tangkai, batang, daun, ranting maupun buah tomat. Serangan pada benih dapat menyebabkan benih rebah, bercak atau benih menjadi busuk. Penggunaan pestisida nabati atau ekstrak tanaman untuk mengendalikan penyakit pada beberapa jenis tanaman telah banyak digunakan. Salah satu bahan alam yang dapat digunakan sebagai pestisida nabati ialah tanaman binahong (Anredera cordifolia) yang merupakan tanaman yang populer digunakan sebagai obat tradisional untuk kesehatan maupun sebagai bahan antimikroba. Penelitian ini bertujuan untuk menguji kemampuan ekstrak kasar umbi udara binahong dalam menghambat pertumbuhan koloni jamur A. solani serta menekan kejadian penyakit pada bibit tomat.  Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri atas  tujuh perlakuan (konsentrasi ekstrak 0,25%, 0,5%, 1%, 2%, dan 4%; kontrol tanpa ekstrak; dan fungisida propineb 0,3%) yang diulang sebanyak empat kali. Pengujian penghambatan pertumbuhan koloni jamur A. solani dilakukan dengan teknik makanan beracun. Pengujian pada benih dilakukan dengan empat perlakuan dan lima ulangan yang masing-masing terdiri atas 25 benih tomat  dengan menggunakan teknik perendaman. Hasil pengujian menunjukkan ekstrak kasar umbi udara binahong 2% mampu menghambat pertumbuhan koloni A. solani sebesar 37,22% dan menekan kejadian penyakit pada bibit tomat dengan penekanan mencapai 83,33%.
Uji In-Vitro Kemampuan Ekstrak Metanol Bunga dan Daun Tanaman Kembang Telang (Clitoria ternatea L.) dalam Menghambat Pertumbuhan Jamur Fusarium oxysporum f.sp. cepae Tarkus Suganda; Indah Nita Chrysilla Simarmata; Yadi Supriyadi; Endah Yulia
Agrikultura Vol 30, No 3 (2019): Desember, 2019
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v30i3.24031

Abstract

Penyakit moler (Fusarium oxysporum f.sp. cepae, Foc) merupakan salah satu kendala utama dalam budidaya tanaman bawang merah. Jamur Foc merupakan patogen tular tanah sehingga sulit untuk dikendalikan. Pengendalian menggunakan fungisida sintetik pada tanah tidak dianjurkan untuk dilakukan karena dapat meninggalkan residu yang berbahaya bagi lingkungan dan kesehatan manusia serta dapat mengganggu keseimbangan ekosistem. Salah satu alternatif pengendalian yang dapat dikembangkan adalah penggunaan fungisida nabati. Kembang telang merupakan tanaman potensial untuk digunakan karena memiliki kandungan senyawa antimikroba. Penelitian ini bertujuan untuk menguji kemampuan ekstrak metanol bunga dan daun tanaman kembang telang dalam menekan pertumbuhan Foc secara in vitro. Percobaan disusun dalam Rancangan Acak Lengkap (RAL) dengan pengujian ekstrak metanol bunga dan daun terhadap pertumbuhan koloni, produksi konidia dan perkecambahan konidia jamur. Hasil percobaan menunjukkan bahwa ekstrak metanol bunga memberikan penekanan tertinggi pada konsentrasi 1,8% (35,11%), sedangkan ekstrak metanol daun pada konsentrasi 2,4% (47,11%). Semua konsentrasi ekstrak metanol bunga dan daun tanaman kembang telang menekan produksi konidia jamur Foc. Tidak ada perlakuan ekstrak metanol, baik bunga maupun daun yang dapat menghambat perkecambahan konidia jamur Foc.