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All Journal Jurnal Fitopatologi Indonesia Agrikultura Cropsaver : Journal of Plant Protection Jurnal Perlindungan Tanaman Indonesia Jurnal Mikologi Indonesia Jurnal Entomologi Indonesia Soil REns 3BIO: Journal of Biological Science, Technology and Management

Sri Hartati

Fakultas Pertanian, Universitas Padjadjaran

Author-ID : 812381

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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Potensi Cuka Kayu Pinus dalam Pengendalian Penyakit Antraknosa pada Cabai Merah Sri Hartati; Rika Meliansyah; Lindung Tri Puspasari
Jurnal Fitopatologi Indonesia Vol 9 No 6 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Anthracnose disease caused by Colletotrichum capsici is a major disease on red chili which causes great economic losses. Wood vinegar, as a result of condensation of smoke, can be used as bactericide and fungicide. The use of pine wood vinegar is prospective for controlling anthracnose disease. The objective of this research was to study the potency of pine wood vinegar in controlling anthracnose on red chili. In vitro test was carried out to measure the inhibition of pine wood vinegar against the growth of mycelium mass and the germination of the conidium of C. capsici. The treatments were the concentration of the pine wood vinegar, i.e. 0, 1.5, 2.5, 3.5, 4.5, and 5 %, and a fungicide (2% of antracol) as comparison. The results showed that the lower the concentration of the pine wood vinegar, the lower the inhibition of the mycelium growth. The highest inhibition of the mycelium growth (76.1%) was caused by 5% of the pine wood vinegar, followed by 41.04% inhibition caused by 4.5% concentration. There was no germination of the conidium on all treatments.

Potency of Rhodotorula minuta as Plant Growth Promoter on Red Chili Plant Infected by Colletotrichum acutatum Sri Hartati; Linda Tarina; Endah Yulia; Luciana Djaya
CROPSAVER Vol 2, No 2 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Some yeast species were reported to have the ability to promote plant growth. Rhodotorula minuta is a yeast species that has the potency as biocontrol agent. This research was objected to evaluate the potency of R. minuta in promoting the growth of red chilli infected by Colletotrichum acutatum. The experiment was carried out at the Laboratory of Plant Protection Biotechnology and Green House of Ciparanje Experiment Station, Faculty of Agriculture, Universitas Padjadjaran. The experiment was arranged in the randomized completely block design with 9 treatments and 5 replications. The treatments were infestation of R. minuta and inoculation of C. acutatum on 5, 7, and 10 days after replanting, and a positive control with no R. minuta infestation but inoculated with the pathogen and a negative control without R. minuta infestation nor pathogen inoculation. Results of the experiment showed that the effectiveness of the treatments with R. minuta on the increase of the chilli plant height was 3.78-17.90%, leaf number was 4.09-15.19%, and dry weight of the biomass was 0.14-0.58%. This results indicated that treatments with R. minuta were able to promote the growth of chilli plants.

The Abilities of Bacteria and Yeast Isolated from Vermicompost Water Extract to Inhibit Alternaria solani in vitro and Early Blight Disease on Tomato Noor Istifadah; Retno Anjani Putri; Sri Hartati
CROPSAVER Vol 4, No 2 (2021)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v4i2.37374

Early blight disease (Alternaria solani Sor) is one of limiting factors in tomato production. Bacteria and yeast are potential for biocontrol of plant diseases. Sources to obtain biocontrol agens is water extract of organic matters. The objective of this study was to examine the abilities of bacteria and yeast isolated from vermicompost water extract in inhibiting the growth of A. solani in vitro and suppressing the pathogen infection in tomato fruits and leaves. Two types of vermicompost used in this study were cattle manure vermicompost and goat manure vermicompost. The isolation of bacteria and yeast from the vermicompost water extract resulted in 14 isolates, which were 10 isolates from cattle manure vermicompost and four isolates from goat manure vermicompost. Among the isolates, six isolates (three yeast and three bacterial isolates) inhibited the growth of A. solani in vitro by 42.8% – 79.1%. In tomato fruits, five isolates inhibited A. solani infection by 70.6% - 100.0%. In tomato plants, four isolates suppressed early blight disease in tomato leaves by 56.2% - 83.5%. The isolate that showed consistent effects in vitro as well as in vivo was bacterial isolate KB 3. This isolate was potential as biocontrol agent of tomato diseases caused by A. solani.

Pengaruh Aplikasi Kompos Gulma Siam Chromolaena odorata terhadap Produksi Senyawa Metabolit Sekunder sebagai Ketahanan Tanaman pada Tanaman Cabai Vira Kusuma Dewi; Nugroho Susetya Putra; Benito Purwanto; Santika Sari; Sri Hartati; Lilian Rizkie
Soilrens Vol 17, No 1 (2019)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Chromolaena odorata (L.) is a organic material as potential compost and botanical pesticides since it compounds have the potential as a pesticides is secondary metabolite. The aim of this research was to know the effects of siam weed Chromolaena odorata compost on production of secondary metabolites in the chili. The experiment was arranged in complete randomized design consisted of four treatments (no fertilizer, cow manure, siam weed compost and inorganic fertilizers) and four replications. The research was done by comparing the chemical content of leaves analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) and phytochemical test. This study was conducted from September 2010 to Februari 2011, in the green house of Departement Plant Pest & Diseases, Faculty of Agriculture. In order to the extraction and characterization of secondary metabolites was conducted at the Laboratory of Chemistry, Universitas Gadjah Mada. Overall, the results of study showed that C. odorata compost could give positive effect on production of secondary metabolites as follow terpenoid, alkaloid, steroid and flavonoid on chili

Pengaruh Pupuk Organik Gulma Siam (Chromolaena odorata L.) dan Ampas Bungkil Mimba (Azadiracta indica A.Juss) pada Sifat Kimia Tanah dan Performa Tanaman Padi Hitam Rizky Fauzi; Octaviani .; Reynaldi Yunior; Vira Kusuma Dewi; Santika Sari; Sri Hartati; Dwi Harya Yudistira; Yongki Umam Sandi
Soilrens Vol 16, No 2 (2018)
Publisher : Fakultas Pertanian Universitas Padjadjaran

ABSTRACT A black rice cultivation study was conducted at Kampung Cinenggang, Cileles Villages, Jatinangor. This research analyzed the effect of organic fertilizer such as siam weed compost and neem cake waste to black rice growth and soil chemicals. The experiment was arranged in randomized block design consisted of five treatments and six replications as follow : neem cake waste , siam weed compost, cow manure, urea were used as the N sources by comparing with no application (control). Overall the result of the study showed that there were no significant differences among treatment in plant growth characters such as plant height and SPAD, except control. In tiller number, the effect of neem cake waste and siam weed compost were significant different if compared with other treatments. Furthermore, the effect of neem cake waste gives a positive effects to soil chemicals which in turn enhanced the growth of black rice. it is recommended that organic fertilizers (neem cake waste and siam weed compost) be utilized instead of chemical fertilizer. Keywords: black rice , neem cake waste, organic fertilizer, siam weed compost, plant growth, soil chemicals

Keefektifan Bakteri dan Khamir Asal Air Rendaman Kompos dalam Menekan Perkembangan Penyakit Bercak Coklat (Alternaria solani Sorr.) pada Tomat Noor Istifadah; Putu Ghita Novilaressa; Fitri Widiantini; Sri Hartati
Agrikultura Vol 31, No 1 (2020): April, 2020
Publisher : Fakultas Pertanian Universitas Padjadjaran

Penyakit bercak coklat yang disebabkan oleh jamur Alternaria solani Sorr. merupakan salah satu penyakit penting pada tanaman tomat. Cara pengendalian penyakit bercak coklat yang biasa dilakukan adalah dengan penyemprotan fungisida sintetik. Mengingat berbagai dampak negatif dari penggunaan pestisida yang terus-menerus, maka perlu dikembangakan cara pengendalian ramah lingkungan seperti pengendalian secara biologi. Bakteri dan jamur merupakan mikrob yang berpotensi sebagai agens biokontrol penyakit tanaman. Salah satu sumber dari agens antagonis patogen tanaman adalah air rendaman kompos. Paper ini mendiskusikan hasil penelitian yang mengevaluasi kemampuan bakteri mikrob yang diisolasi dari air rendaman kompos berbahan dasar kotoran sapi dan domba untuk menghambat pertumbuhan A. solani in vitro dan menekan penyakit yang disebabkan patogen tersebut pada buah dan tanaman tomat. Percobaan secara in vitro menggunakan Rancangan Acak Lengkap, sementara pengujian pada buah dan tanaman tomat menggunakan Rancangan Acak Kelompok. Isolasi mikrob dari air rendaman kompos berbahan dasar kotoran sapi dan domba menghasilkan 35 isolat, yang mana 11 isolat (enam isolat bakteri dan lima isolat khamir) dapat menghambat pertumbuhan A. solani secara in vitro sebesar 79,3%-84,2% dengan zona hambat sebesar 0,0-28,3 mm. Pada pengujian secara in vivo, lima isolat non-patogenik (dua isolat bakteri dan tiga isolat khamir) dapat menekan penyakit bercak coklat pada buah tomat sebesar 100% dan pada daun tomat sebesar 77,5%-98,1%. Isolat-isolat ini berpotensi untuk dikembangkan lebih lanjut sebagai agens biokontrol penyakit bercak coklat pada tanaman tomat.

Kompatibilitas Vegetatif Fusarium oxysporum dari Beberapa Tanaman Inang Sri Sri Hartati; Ummu Salamah Rustiani; Lindung Tri Puspasari; Wawan Kurniawan
Agrikultura Vol 27, No 3 (2016): Desember, 2016
Publisher : Fakultas Pertanian Universitas Padjadjaran

ABSTRACTVegetatif compatibility of Fusarium oxysporum on various hostsMany strains or race of Fusarium oxysporum can be grouped based on compatibility reproduction from a variety of different strains called Vegetative Compatibility Group (VCG). This study was aimed to determine how the grouping of several isolates of F. oxysporum and grouping of several hosts of the fungus by vegetative compatibility group. Fusarium oxysporum isolated from chickpea plants that showed symptoms of fusarium wilt. The isolates of F. oxysporum of chili and tomatoes obtained from the culture collections of Mycology Laboratory of IPB. Stages of vegetative compatibility testing assayed through recovery of nit mutants, the identification of phenotype of nit mutant, and complementation test. There are 29 mutants isolated from the isolates of F. oxysporum. Nit1 mutant was obtained from all isolates of beans, tomatoes and peppers. NitM and Nit3 mutant isolates were obtained from chickpea 4 and chili sequentially. Two VCG and one single self compatibility (SSC) were assayed from isolates of F. oxysporum based on complementation testing.Keywords: Beans, Fusarium wilt, Nit mutant, SSC, VCGABSTRAKJamur Fusarium oxysporum memiliki banyak forma spesialis dan ras. Jamur ini dapat dikelompokkan berdasarkan kompatibilitas reproduksi dari berbagai strain yang berbeda disebut dengan vegetative compatibility group (VCG). Penelitian ini bertujuan untuk mengetahui cara pengelompokkan F. oxysporum dan pengelompokkan jamur tersebut dari beberapa inang berdasarkan kelompok kompatibilitas vegetatifnya. Isolasi F. oxysporum dilakukan dari tanaman kacang panjang yang menunjukkan gejala layu fusarium. Isolat F. oxysporum dari cabai dan tomat berasal dari koleksi Laboratorium Mikologi IPB. Tahapan pengujian kompatibilitas vegetatif melalui pembiakan nit mutan, identifikasi fenotipe nit mutan, dan pengujian komplementasi. Isolasi mutan F. oxysporum didapatkan 29 mutan. Mutan nit1 didapatkan dari semua isolat yang diperoleh dari semua inang yang berbeda yaitu kacang panjang, tomat dan cabai. Mutan nitM hanya didapatkan dari isolat kacang panjang 4 dan mutan nit3 hanya didapatkan dari isolat cabai. Berdasarkan uji komplementasi F. oxysporum yang diuji terdiri dari dua VCG dan satu single self compatibility (SSC).Kata Kunci: Kacang panjang, Layu fusarium, Nit mutant, SSC, VCG

Penambahan Gula pada Medium Biakan untuk Meningkatkan Pertumbuhan dan Kemampuan Antagonisme Candida tropicalis terhadap Patogen Penyebab Penyakit Layu Fusarium Tomat Sri Hartati; Timotius A. Sumbari; Ceppy Nasahi; Wawan Kurniawan
Agrikultura Vol 31, No 2 (2020): Agustus, 2020
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v31i2.28544

Layu fusarium merupakan salah satu penyakit utama pada tanaman tomat yang disebabkan oleh Fusarium oxysporum f.sp. lycopersici (FOL) Salah satu agens antagonis yang dapat menghambat pertumbuhan FOL adalah khamir Candida tropicalis. Pertumbuhan dan kemampuan antagonisme khamir dapat ditingkatkan dengan penambahan gula sebagai sumber nutrisi. Penelitian ini bertujuan untuk mendapatkan konsentrasi dan jenis gula yang dapat meningkatkan pertumbuhan dan antagonisme C. tropicalis terhadap FOL. Percobaan dilakukan dengan menggunakan Rancangan Acak Lengkap yang terdiri atas 16 perlakuan dan 3 ulangan. Perlakuan terdiri atas jenis gula yang ditambahkan pada medium tumbuh khamir yaitu glukosa, sukrosa, trehalosa, D-xylose, dan D-mannitol dengan konsentrasi masing-masing gula 1%, 2,5%, 5%, dan kontrol (PDA setengah dekstros dan PDA dekstros penuh). Hasil percobaan menunjukkan bahwa D-mannitol 1% mampu meningkatkan panjang koloni C. tropicalis tertinggi sebesar 4,80 cm, dibanding kontrol (4,07 cm). Sukrosa 2,5% mampu meningkatkan lebar koloni tertinggi sebesar 1,47 cm, dibanding kontrol (1,00 cm). Seluruh perlakuan mampu meningkatkan kerapatan sel khamir C. tropicalis. Kerapatan sel tertinggi dihasilkan oleh trehalosa 1% sebesar 4,27 x 107, sementara pada kontrol 0,1 x 107 sel/ml. Gula trehalosa 2,5% dapat meningkatkan antagonisme khamir dengan daya hambat tertinggi sebesar 23,68%, dibandingkan dengan kontrol PDA dekstros penuh.

Komposisi Komunitas Serangga Aphidophaga dan Coccidophaga pada Agroekosistem Kacang Panjang (Vigna sinensis l.) di Kabupaten Garut Lindung Tri Puspasari; Martua Suhunan Sianipar; Sri Hartati
Agrikultura Vol 27, No 1 (2016): April, 2016
Publisher : Fakultas Pertanian Universitas Padjadjaran

ABSTRACTComposition of Aphidophaga and Coccidophaga Insect Communities on Long Bean Agroecosystem (Vigna sismensis L.) at Garut RegencyThe research about Aphidophaga and Coccidophaga insect composition comunity on long beans (Vigna sinensis L.) agroecosystems was done in the month of April to November 2011 in Haruman Village, Leles District, Garut Regency. Research was conducted in the form of surveys which were done by collecting insects directly and using various traps ie yellow traps board, fitfall traps, and nets swinging. The dominant insect pest that found was Aphis craccivora which causing percentage of damage to the plants ranging from 20% to 90%. Types of aphidophaga and coccidophaga found were belong to the Order of Coleoptera : Family Coccinellidae Menochilus sexmaculatus, Micraspis sp., Harmonia sp., Verania lineata, Curinus coeruleus, Scymnus sp., Coccinella transversalis); the Order of Diptera : Family Syrphidae namely Ischiodon scutellaris; Neuroptera: Family Hemerobiidae; the Order of Diptera : Family Cecidomyiidae Aphidoletes aphidimyza; and there was also Carabidae beetles of the Order Coleoptera with species diversity index wasl relatively low. The highest abundance of predators of coccidophaga and aphidophaga group was dominated by Ischiodon scutellaris and Menochilus sexmaculatus.Key words: Diversity, Abundance, Dominant species, Insect pestsABSTRAKPenelitian mengenai komposisi komunitas serangga aphidophaga dan coccidophaga pada Agroekosistem kacang panjang (Vigna sinensis (L.) telah dilaksanakan pada Bulan April–November 2011 di Desa Haruman Kecamatan Leles Kabupaten Garut. Penelitian dilakukan menggunakan metode survei dengan mengoleksi serangga secara langsung, dan menggunakan berbagai perangkap antara lain perangkap papan kuning, fitfall trap, dan jaring ayun. Serangga hama dominan yang ditemukan adalah Aphis craccivora dengan persentase serangan berkisar 20% - 90%. Jenis aphidophaga dan coccidophaga lain yang ditemukan yaitu dari Ordo Coleoptera : Famili Coccinellidae: Menochilus sexmaculatus, Micraspis sp., Harmonia sp., Verania lineata, Curinus coeruleus, Scymnus sp., Coccinella transversalis; Ordo Diptera : Famili Syrphidae yaitu Ischiodon scutellaris; Neuroptera : Famili Hemerobiidae; Ordo Diptera : Famili Cecidomyiidae Aphidoletes aphidimyza; serta terdapat pula kumbang Carabidae dari Ordo Coleoptera dengan indeks keragaman spesies yang masih tergolong rendah. Kelimpahan tertinggi predator dari kelompok aphidophaga dan coccidophaga didominasi oleh spesies Ischiodon scutellaris dan Menochilus sexmaculatus.Kata kunci : Keragaman, Kelimpahan, Spesies dominan, Serangga hama

Identifikasi Isolat Khamir Berpotensi sebagai Agens Antagonis dan Uji Produksi Toksin Hemolisin Sri Hartati; Suryo Wiyono; Sri Hendrastuti Hidayat; Meity Suradji Sinaga
Agrikultura Vol 32, No 2 (2021): Agustus, 2021
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v32i2.33849

Identifikasi khamir dapat dilakukan secara konvensional maupun molekuler. Identifikasi secara konvensional membutuhkan waktu yang lama dan interpretasi hasilnya seringkali bersifat subyektif. Sementara identifikasi khamir dengan metode molekuler dapat memberikan hasil yang lebih akurat dan cepat. Khamir yang berperan sebagai agens antagonis harus aman terhadap organisme nontarget agar dapat diaplikasikan di lapangan. Penelitian ini bertujuan untuk mengidentifikasi isolat-isolat khamir berpotensi antagonis dengan metode molekuler dan mengetahui kemampuan khamir dalam menghasilkan hemolisin sebagai salah satu indikator potensi resiko terhadap mamalia. Identifikasi dan pengujian kemampuan khamir dalam menghasilkan hemolisin dilakukan pada 15 isolat khamir berpotensi antagonis terhadap patogen antraknosa cabai (Colletotrichum acutatum). Identifikasi khamir dilakukan secara molekuler dengan PCR menggunakan primer ITS1 dan ITS4. Penyediaan khamir menggunakan mediaYeast Malt Extract Broth (YMB) dan Potato Dextrose Agar (PDA). Pengujian kemampuan khamir dalam menghasilkan hemolisin menggunakan media blood agar base (Oxoid CM55) ditambah darah domba 5%. Hasil identifikasi menunjukkan bahwa isolat khamir dapat teramplifikasi dengan primer ITS1 dan ITS4 dengan ukuran fragmen produk antara 500-800 pb. Hasil analisis sekuensing didapatkan 6 spesies khamir yaitu Candida tropicalis, Rhodotorula minuta, Aureobasidium pullulans, Pseudozyma hubeiensis, Pseudozyma aphidis, dan Pseudozyma shanxiensis. Uji kemampuan khamir dalam menghasilkan hemolisin menunjukkan bahwa seluruh khamir yang diuji tidak menghasilkan toksin hemolisin sehingga diduga isolat-isolat tersebut tidak patogenik terhadap manusia.

Co-Authors Agus Dana Permana Agus Susanto Agus Susanto Bari, Ichsan Nurul Benito Heru Purwanto Ceppy Nasahi Desy Natalia Br. Saragih Dian Nurul Izzati Dwi Harya Yudistira Endah Yulia Fitri Widiantini Lilian Rizkie Linda Tarina Linda Tarina Linda Tarina Lindung Tri Puspasari Luciana Djaya Luciana Djaya M S Sinaga Martua Suhunan Sianipar Meity Suradji Sinaga Meity Suradji Sinaga Mustika Dewi Noor Istifadah Nugroho Susetya Putra Nurul Falah Khomaedy Octaviani . Putu Ghita Novilaressa Ramadhan Kamal Putra Retno Anjani Putri Reynaldi Yunior RIKA MELIANSYAH Risma Yuniah Nur’haqi Rizky Fauzi Rizky Fauzi S H Hidayat S Wiyono Santa Monica Santika Sari Santika Sari Siska Rasiska SRI HENDRASTUTI HIDAYAT Sri Rosmala Dewi Suryo Wiyono Syifa Nabilah Subakti Putri Tarkus Suganda Tiara Febricyanti Putri Senjaya Timotius A. Sumbari Tohidin Tohidin Ummu Salamah Rustiani Vira Kusuma Dewi Wahyu Daradjat Natawigena Wahyu Daradjat Natawigena Wawan Kurniawan Yani Maharani Yongki Umam Sandi Yongki Umam Sandi Yudi Rismayadi Yulia, Endah Yulia

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