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INDONESIAN JOURNAL OF PHARMACY

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Published by Universitas Gadjah Mada

ISSN : 23389427 EISSN : 23389486 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.

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Articles 706 Documents

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n-Butanolic fraction of endofitic fungi of Buah Makasar increases apoptotic effect of doxorubicin on MCF-7 cells Meiyanto, Edy; Kumala, Shirly; Septisetyani, Endah Puji
Indonesian Journal of Pharmacy Vol 20 No 1, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Makassar fruit, Brucea javanica (L.) Merr., showed chemopreventive activity. Secondary metabolites come from B. javanica fruit, brucatol and bruceantine, induced cell differentiation and apoptosis on Leukemia cell, while quassinoid and its derivates acted as antitumor promoter. Butanolic fraction of supernatan of endofitic fungi 1.2.11 isolate fermentation which isolated from B. javanica fruit showed cytotoxicity toward several cancer cells. This fraction has been predicted contain secondary metabolites from B. javanica and has been identified as Bruceosin and Canthin-6-one derivates. Butanolic fraction (FB) of supernatan from endofitic fungi 1.3.11 isolate fermentation is predicted for having similiar cytotoxycity as active as 1.2.11 isolate. This research is aimed to explore cytotoxycity potention dan doxorubicin on MCF-7 breast cancer cell.Synergism of BF-doxorubicin combination detect from cell viability inhibition and apoptosis induction on MCF-7, a breast cancer cell lines which shows resistancy toward doxorubicin. Cell viability on single treatment of FB and doxorubicin and its combination were carried out by MTT assay to determine IC50 and combination index (CI). Apoptosis induction of FB, doxorubicin and its combination were carried out by ethidium bromideacridine orange DNA staining.n- Butanolic fraction and doxorubicin showed cell viability inhibition on MCF-7 cell with IC50 48 μg/mL and 148 nM, respectively. Both of FB and doxorubicin showed apoptosis induction on IC50. Combination of FBdoxorubicin showed synergism and increased apoptosis induction on MCF-7 cell.Key words: Brucea javanica, endofitic fungi, MCF-7 cell, synergism, doxorubicin.

DUAL EFFECTS OF FLAVONOID QUERCETIN ON RAT BASOPHILIC LEUKEMIA (RBL-2H3) CELLS : INHIBITS HISTAMINE RELEASE AND REDUCES CELL GROWTH Zullies Ikawati; Elizabeth Wallgard; Kazutaka Maeyama
Indonesian Journal of Pharmacy Vol 13 No 2, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The dual effects of the flavonoid quercetin on rat basophilic leukemia cells (RBL-2H3), tumor analog mast cells, was studied. Quercetin is known as an anti-inflammatory drug that inhibits mast cell secretion of histamine. This study aims to investigate the consequences of varying incubation times with quercetin on 2H3 cells to histamine release inhibitory action and the effects of long time incubation to the morphology of the cells. The effect of quercetin on histamine synthesis was also observed. The histamine release from the cells was inhibited by quercetin as expected, but the ability of secretion was rapidly recovered when quercetin was removed before challenging. Incubation up to 6 hours decreased the inhibitory action, but longer than 6 hours increased the inhibitory activity. In long time incubation, the cells exhibited cell damage, decreased cell growth, morphological changes, and detachment from the underlying surface in proportion with the concentration of quercetin. The instant and reversible inhibitory effects of quercetin appear to represent a first phase of actions, while reduced cell growth, elevated cell damage and morphological changes seem to be connected to a second phase. Consequently, quercetin could be considered as a compound that acts dually on RBL-2H3 cells.Key words : quercetin, histamine, RBL-2H3 cells

Effect of protein fraction of Carica papaya L. leaves on the expressions of p53 and Bcl-2 in breast cancer cells line Rumiyati .; Sismindari .; Ariyani .
Indonesian Journal of Pharmacy Vol 17 No 4, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Carica papaya L. leave was known containing Ribosome-inactivating proteins (RIPs), which demonstrated to have an in vitro cytotoxic effect on cancer cell lines. This researh examined the effect of protein fraction containing RIPs isolated from Carica papaya L. on the expressions of p53 and Bcl-2, regulator proteins on apoptosis process, in breast cancer cell lines (T47D).RIPs from Carica papaya L. leaves were isolated by amonium sulfat precipitation. This fraction was analyzed by the activity of cleaving supercoiled double stranded DNA, in order to identify the presence of RIP. The active fraction was then tested of the citotoxic activity on breast cancer cell lines followed by analysing the expressions of p53 and bcl2 using immunohistochemistry technique.The results indicated that the protein fraction possessed cytotoxic activity in breast cancer cell line with the IC50 of 2.8 mg/mL. The expression level of p53 was increased by 59.4%, while Bcl-2 protein was \ decreased by 63%. These results suggested that this protein could induce apoptotic process.Key words : protein fraction, Carica papaya L. , expression of p53 and Bcl-2

Labelling of human serum albumin (HSA)-nanospheres with technetium-99m radionuclide Oekar, Nanny Kartini; Widyasari, Eva Maria
INDONESIAN JOURNAL OF PHARMACY Vol 19 No 3, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Labelling of HSA-nanospheres with technetium-99m was carried out by direct and indirect method using sodium pyrophosphate as co-ligand agent. The labelling efficiency was determined by several chromatography system for separation of 99mTc-HSA-nanospheres labelled compound from its radiochemical impurities. Several parameters influencing the labelling process were studied such as pH, kinds and quantity of reductor agent, labelling method, quantity of HSA-nanospheres and temperature and the duration of incubation. The result show that the optimum direct labelling condition was found by using 0.5 mL HSA-nanospheres solution (which had absorbance of 0.6 at l= 202 nm), 100-150 Î¼g of SnCl2.2H2O as reductor, pH mixture was 2 and the first incubation was done at room temperature for 25 minutes. The labelling process was continued by adding technetium-99m of certain activity, and finally pH was adjusted to 5.5-6.0. The second incubation was carried out at room temperature for 15 minutes. This direct method resulted more than 90% of labelling efficiency, with free 99mTc-pertechnetate as radiochemical impurity. The indirect labelling process by using 0.5 mL of HSA-nanospheres solution, 100 Î¼g of SnCl2.2H2O was prior reacted with 1.0 mg of sodium pyrophosphate (1 : 5 mol/mol), the pH was adjusted to 7.4 and incubation was done in the incubator at 37oC for 15 minutes. After adding 99mTc-pertechnetate solution, the second incubation was done at room temperature for 15 minutes. This indirect labelling resulted 93.4 Â± 1.2 % of labelling efficiency with remain of 99mTc-pertechnetat and 99mTc-pyrophosphate as radiochemical impurities.Key words: lymphoscintigraphy, nanocolloid, technetium-99m, radiochemical impurities.

DASYPOGALACTONE, A NEW CHIRALIC C3-SYMMETRIC MACROLACTONE FROM LICHEN USNEA DASYPOGA ROHL. Ratna Layla Gani; Wahyudi Priyono Suwarso; Karsten Krohn; Markus John
Indonesian Journal of Pharmacy Vol 12 No 3, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Medicinal plants spread out in Indonesia, however only a part of them have been clinically investigated that exhibited therapeutic effecs. This research was to isolate a new compounds in Usnea dasypoga. A new twentyfour-membered macrolactone composed of three units of unknown fatty acid (2R*,3R*,4S*,7S* or 2S* 3S* 4R*, 7R*)-3,7-dihydroxy-2,4-dimethyloctanoic acid forming a C3-symmetrical lactide was isolated from Indonesian lichen Usnea dasypoga Rohl. The result shown that those compound had been confirmed with the spectroscopic data.Keywords: Natural products isolation, three lactide, lichen

Analysis of Derivate Compound Between Sodium Alendronat with Chloride by High Performance Liquid Chromatography Yahdiana Harahap; Hayun .; Meriska Sukandar
Indonesian Journal of Pharmacy Vol 18 No 2, 2007
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Sodium alendronate is one of biphosphonate group drugs for the treatment of osteoporosis,. The aim of this research was to obtain the optimum condition for forming derivative of sodium alendronate with dansyl chloride. By adding 270 μL dansyl chloride to 0.1 M sodium carbonate buffer at pH 10.0 mixing with thermomixer at 50.oC for 50 minutes, resulted in a stable derivative within 30 minutes. The compound was analysed by high performance liquid chromatography method using C18 column acetonitrilemethanol-buffer (25 mM KH2PO4 and 25 mM citric acid) (20:15:65;v/v) as mobile phase at a flow rate of 1.0 mL/minute; (detected at wavelength of excitation 320 nm and emission 495 nm). The retention time of the derivative was 19.758 minutes, the calibration’s curve was linear at concentration range of 0.2-1 μg/mL with coefficient of correlation (r) 0.9995 and limit of quantitation 0.114 μg/mL.Key word : HPLC, sodium alendronat, dansyl chloride, fluorescence

Niosomes entrapment capacity of ketoprofen and prediction transdermal administration rahman, Latifah; Ismail, Isriany; Wahyudin, Elly
Indonesian Journal of Pharmacy Vol 22 No 2, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Niosomes are vesicles system that have applications in the delivery of lipophilic,hydrophilic and amphiphilic drugs. Ketoprofen, is very insoluble in water and cause gastric irritation when taken orally. It is very important to develop a transdermal delivery system for ketoprofen. This research was aimed to design niosomes which can deliver ketoprofen via transdermal route. Experiments were designed to incorporate ketoprofen into niosomes with lipid film hydration method. Lipid mixture consist of cholesterol and sorbitan ester (span 20, 60, 80). Niosomes which can deliver ketoprofen trough the skin barrier determined by calculating amount of ketoprofen in the blood of rabbit. The type of sorbitan ester was chosen based on the highest drugs entrapment and ketoprofen as drugs model. Preparation of niosomes was optimized for the highest percent drug entrapment by increasing molar concentration of lipidmixture with the stable comparison of 1:1. This research result are niosomes with lipid mixture span 60 and cholesterol have the highest drug entrapment efficiency of niosomes 66.16% with range size 1–6 µm. Niosomes can deliver ketoprofen to the systemic circulation via transdermal route with plasma level concentration achieved in 1.5 hour.Key words: niosomes, ketoprofen, transdermal

ISOLATION AND IDENTIFICATION OF ANTIFUNGAL (Candida albicans) COMPOUND FROM THE HULL OF DELIMA FRUITS (Punica granatum L.) Purwantini, Indah; Wahyuono, Subagus
Indonesian Journal of Pharmacy Vol 14 No 3, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The hull of delima fruits (Punica granatum L.) are traditionally used to cure dysmenorhoe. Preliminary study indicated that extract of the hull was able to inhibit the growth of Candida albicans. Therefore this study was aimed to isolate and identify active compounds responsible for the activity from the hull of P. granatum. The study was initiated by extracting the powdered material with petroleum ether followed by methanol. Antifungal activity test (100 mg/ml) indicated that the petroleum ether extract was more active than the methanol extract (inhibition zone: 10.59 vs 6.92 mm). The pet. ether extract was triturated by n-hexane to give n-hexane insoluble and n-hexane soluble fractions. The latter that was active (inh. zone: 9.50 vs 0.00 mm) was fractionated by vacuum liquid colomn chromatography (vlc; SiO2, n-hexane with increasing amount of ethylacetate) to give 7 fractions (F1-F7). Fraction 2 (inh. zone: 9.05 mm) and 3 (11.05 mm) displayed antifungal activity, then F3 was subjected to contact bioautography to give 2 active compounds [Rf. 0.50 (major) and 0.10 (minor)]. Preparative tlc [SiO2 F-254 nm; nhexane: ethylasetate 4-1, developed 2x) of F3 was aimed to separate 2 active compounds. Due to limited amount of the minor compound, the MFC was applied only for the major compound (Rf. 0.50; 200 mg/ml). The structure identification was done by mean of spectroscopic methods (uv, ir, ms and nmr) to be a setrol type of compound having stigmastane skeleton, esterified by a long-chain fatty acid.Keywords: Punica granatum L., Candida albicans, active compound, terpenoid

Sequencing analysis of the hole DNA of a mutant gene of Klebsiela pneumoniae resistant against BRL 41897A (KSL 19 Mutant) M. Kuswandi
Indonesian Journal of Pharmacy Vol 17 No 2, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Previous article reported that three positive transformants of different K.pneumonie resistant mutants against BRL 41897A (KSL 19, KSL38 and KSL62) carrying TnphoA was sequenced. The results showed that the three different transformants of the mutants had different genes inserted TnphoA. Subsequently, we then cloned the wild typeM10 chromosome fragments into pUC18 and recombinants analysed by gel electrophoresis and Southern blotting using HindIII fragment from KIH 19 as aprobe. The clones were designated M19 carrying the hole gene which had an important role in the antibiotic resistantion. The plasmid DNA of positive clone was isolated, analysed by Southern blotting, and sequenced using primers based on sequencing data from KIH19. Sequencing data of KIH19 confirmed the sequence of M19 and the point of insertion of transposon. The amino acid sequence at the N terminus of disrupted gene ksl19 showed the characteristics of features of signal peptide structure. In order to characterise the M19 clone, further complementation analysis was carried out, by transformation of M19 plasmid into KSL19 mutant cell, designated T19, and then checked by gel electrophoresis and sensitivity test. The complementation studies showed that sensitivity to BRL 41897A was restored on transformation of a large (7 kb) fragment of DNA carrying ksl19 geneKey words: Resistant K.pneumoniae KSL19 mutants -BRL41897A, sequencing.

Formula optimization of sustained-release ibuprofen tablet using simplex lattice design with mixture of caragenan, calsium sulphate, and PVP-K30 Wuryanto Hadinugroho; Achmad Fudholi
Indonesian Journal of Pharmacy Vol 22 No 4, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Ibuprofen is one of non steroid anti inflammatory drug (NSAID), derivated propionic acid often use rheumatoid therapy. Frequented using can make unpleasant of patient, solution for this problem by sustained release ibuprofen tablet design. This experiment aim is find out the optimized formula of sustained release ibuprofen tablet with hardness of ≥ 4 kgf, brittleness of ≤ 1 %, and 0.7-0.9 mg/minutes ibuprofen rate out by using mixture of carrageenan, calcium sulphate and PVP-K30. This formula use ibuprofen as 400 mg, the mixture of carrageenan, calcium sulphate and PVP-K30 as 12 mg, so tablet total weight is 600 mg, the mixture total weight is. The proportion was optimized by using simplex lattice design special cubic model. There are seven formula in this design (each three formula consist of 100 %, three formula consists of two as 50 % : 50 % and last formula mixture three component as 33.33 % of each) to get coefficient of equation Y = a(A)+b(B)+c(C)+ab(A)(B)+ac(A)(C)+bc(B)(C)+ abc(A)(B)(C). Based of each optimized parameter equation is got contour plot and combined to superimposed contour plot, so the optimized formula can be found. Based of superimposed contour plot is mixture carrageenan:calcium sulphate:PVP-K30 comperation of optimized formula sustained release ibuprofen tablet are 0.347 : 0.233 : 0.420 to find out the optimized formula of sustained release ibuprofen tablet with the hardness as 5.71; brittleness as 0.28; and ibuprofen rate out as 0.79. The most dominant increase factor on hardness is calcium sulphate, on brittleness is carrageenan and on ibuprofen rate out is PVPK30. About interaction, the most hardness is calcium sulphate-PVP-K30, the most brittleness and ibuprofen rate out is carrageenan-calcium sulphate-PVPK30.Key words:carrageenan, calcium sulphate, PVP-K30, ibuprofen.

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