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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
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Articles 9 Documents
Search results for , issue "Vol. 4 No. 3 (2014)" : 9 Documents clear
Evaluation of Antioxidant Activity of Some Selected Tropical Fruits in South Kalimantan, Indonesia Efrilia Tanjung; Muhammad Hafidz MS; Iskandar Thalib; Eko Suhartono
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.04.03.09

Abstract

In the present study antioxidant and antioxidant activity of some tropical fruits was evaluated. Antioxidants are compounds or molecules that can scavenging and prevent free radicals and reactive oxygen species that can caused a cell damage. Fruit was known as a source of antioxidant. South Kalimantan Indonesia, has a variety of fruit such as mentega, nangka, timun suri and kuranji. Study for evaluating the antioxidant levels and activity of those fruit were never been investigated. Thus, our study aim to measure the antioxidant levels and antioxidant activtity of those selected fruits. Ascorbic acid, lycopene, b-carotene levels and antioxidant activtity of four selected tropical fruits was evaluated using spectrophotometer. The result of this studied suggest that the four selected tropical fruits is potential antioxidant because it contained ascorbic acid, b-carotene, lycopene and had effect of scavenging radical hydroxyl, hydrogen peroxide and chellating ferrous iron.
Selecting Tree Species with High Carbon Stock Potency from Tropical Upland Forest of Bedugul-Bali, Indonesia Arief Priyadi; Sutomo Sutomo; I Dewa Putu Darma; Ida Bagus Ketut Arinasa
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Vegetation studies to reveal tree diversity and its contribution to carbon stock were conducted in three differ­ent sites of upland forest in Bali, Indonesia. The sites were located approximately 60 km north of the Bali Province capital city of Denpasar in an area named Bedugul. Those three sites were Mt. Mangu (forest area east of Beratan lake), forest area west of Buyan lake and forest area south of Tamblingan lake. There were 44, 29, and 21 tree species of 14, 19, 14 families with Shannon Diversity Index (H) of 2.87, 2.64 and 1.69 respectively. Carbon stock average of above ground tree biomass from these sites were 214.2, 693.0 and 749.1 ton.ha-1 respectively. Tree species with top Summed Dominance Ratio (SDR) in each of those sites were Platea latifolia in Mt. Mangu, Plan­chonella sp. in Buyan, and Tabernaemontana macrocarpa in Tamblingan. Average carbon content of these three species were 493.25, 12,876.26 and 40.35 kg.ha-1 respectively.
Bovine And Human Zona Pellucida 3 Gene Glycans Site Prediction Using In Silico Analysis Nurul Jadid Mubarakati; Aulanni'am Aulanni'am; Sutiman Bambang Sumitro; Gatot Ciptadi
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.04.03.08

Abstract

Zona pellucida is one of the protective layer of the egg cell and has a function as an intermediary species-specific fertilization. Glycoproteins of human and bovine zona pellucida is composed of three types, namely ZP1, ZP2 and ZP3. ZP3 gene has amino acid sequence homology with other mammals. Oligosaccharides components of the zona pellucida glycans are composed from units of asparagine residues (N -linked) and serine/threonine (O -linked). The aims of this study was to analyze the DNA sequences of human and bovine and further predicts glycans site on amino acid sequence of human and bovine ZP3. In this study, ZP3 gene fragments have been isolated from bovine and humans were analyzed in Silico. This work were conducted by comparing the data of DNA sequence from human and bovine PCR product using NCBI BLAST. The results showed that there were similarities at amino acid positions number 23-38. bZP3 sequence had three glycans site (Asn-X-Thr/Ser) and one site on hZP3 glycans. One of the sites was conserved between the two species.
37% Phosphoric Acid Induced Stronger Matrix Metalloproteinase-8 Expression of the Dental Pulp than 19% Ethylene Diamine Tetraacetic Acid Nadie Fatimatuzzahro; Tetiana Haniastuti; Juni Handajani
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Etching agents such as ethylene diamine tetraacetic acid (EDTA) and phosphoric acid which are widely used in adhesive restoration system aimed to increase for retention of restorative materials, may act a chemical irritant that induce inflammation of dental pulp. Inflammation is a body response against irritant and infectious agents. Matrix metalloproteinase-8, the major collagenolytic enzyme, degrades collagen type 1. This enzyme is expressed in low level in normal condition, however, the expression will increase during inflammation. The purpose of the present research was to study the effect of 19% EDTA and 37% phosphoric acid application as an etching agents on the MMP-8 expression of dental pulp. Forty-five male Sprague Dawley rats were divided into 3 groups. Cavity preparation was made on the occlusal surface of maxillary first molar using a round diamond bur. 19% EDTA, 37% phosphoric acid, and distilled water were applied on the surface of the cavity of the teeth in group I, II, and III subsequently. The cavity then filed by glass ionomer cements. The rats were sacrified at 1, 3, 5, 7, and 14 days after the treatment (n=3 for each day). The specimens were then processed histologically. Immunohistochemical (IHC) analysis was performed using rabbit anti rat MMP-8 polyclonal antibody to examine MMP-8 expression and HE (Hematoxylen Eosin) staining to observe the number of macrophages. The results showed 37% phos­phoric acid application induced stronger expression of MMP-8 and higher number of macrophages than 19% EDTA. The strongest expression of MMP-8 seems on 5 days after the treatment where the highest number of macrophages were also found.
Non-breeding Ethology of Spot-billed Pelican (Pelecanus philippensis) at Adyar Eco-Park, Chennai, India Meghna Bandyopadhyay; Rishin Basu Roy; Arijit Chatterjee
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
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Abstract

Spot-billed Pelican is a Near Threatened species according to IUCN red list. There are very limited studies regarding the non-breeding behavior of the Spot-billed Pelican, where behavioural study is very important to un­derstand the species and ecosystem to which they belong. Studying ethology is an important stepping stone for the conservation of the species and its ecosystem. With this aspect an ethological study was carried out at Adyar Eco-park Chennai during the non-breeding season of Spot-billed Pelican for a period of thirteen days continuously. An Ad libitum study was adapted to document their behaviour. The observation was conducted over a period of 7 different time schedules. From the observation 14 individual behaviours and 4 conspecific and group behaviours were recorded. The four most time consuming activities are Alert (193.13 min, 31.15%), Preening (92.36 min, 14.89%), Resting (81.13 min, 13.09%) and Looking around (71 min, 11.45%). The mean population density in the study area showed a declining trend from dawn to noon (2.04-0.55 individuals per 100 sq. m.) and increasing from noon to evening (0.55-3.81 individuals per 100 sq. m.). During the observation it was found that this area is a non-breeding roosting site for the bird and they show some typical pattern as the day progresses. The present study can be helpful to build a comprehensive conservation programme for the Spot-billed Pelican and management plan for Adyar Eco-park can be developed if carried out for a longer period of time.
Low Levels of Vitamin D correlate with Hemoglobin A1c and Interleukin-10 Levels in Pediatric Type 1 Diabetes Mellitus Patients Desy Wulandari; Haryudi A. Cahyono; Edi Widjajanto; Anik Puryatni
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
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Abstract

Vitamin D is reported to affect immune system and prevent autoimmunity. Some studies show that low vita­min D levels in patients with type 1 diabetes are associated with glycemic control and inflammatory status. The study was cross sectional design with subjects T1DM patients aged 1-18 years, and healthy subjects with similar age. Plasma levels of vitamin D (25(OH)D3) was measured using ELISA. Glycemic control measured by hemoglo­bin A1c (HbA1c). Inflammatory status measured by examination of IL-10 using ELISA method. Comparison of vitamin D levels, HbA1c levels and IL-10 levels were analyzed by independent samples t-test. The relationship be­tween vitamin D levels, HbA1c, and IL-10 were analyzed by Pearson's correlation. Our subjects were 20 T1DM patients and 20 healthy controls. Our study showed that vitamin D (25(OH)D3) levels was significantly lower than in healthy controls (20.82±5.53 ng/ml vs 33.14±2.17 ng/ml; p=0.000), HbA1c levels was significantly higher than in healthy controls (10.08±5.02% vs 5.02±0.18%; p=0.000), IL-10 levels was significantly lower than in healthy controls (16.50±4.57 ng/ml vs 73.52±7.11 ng/ml; p=0.000). Vitamin D levels were correlated with HbA1c (p= 0.000; r= -0.871), correlated with IL-10 (p= 0.000; r= 0.853). HbA1c levels were correlated with IL-10 levels (p= 0.000; r= -0.878). Low vitamin D levels are common in T1DM patients. There were significant differences in vitamin D levels, IL-10, and HbA1c among T1DM patients compared to normal subjects. Vitamin D levels were associated with HbA1c levels and IL-10 levels in T1DM.
Potential Anti-cancer and Anti-bacterial Activities of Philippine Echinoderm Extracts Rodyl J. Layson; Maria Criselda A. Rodil; Elmer-Rico E. Mojica; Custer C. Deocaris
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
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Abstract

In high-throughput search for bioactive compounds under resource-limited settings from Philippine echino­derms, the aqueous, methanol, chloroform and hexane extracts of seven Philippine echinoderms namely Holothuria nobilis (sea cucumber), Bohadscia marmorata (sea cucumber), Stichopus chloronatus (sea cucumber), Holothuria axiologa (sea cucumber), Linckia laevigata (starfish), Oreaster nodusus (starfish) and Ophiocoma ochoenleinii (brittle star) were screened for antitumor and antibacterial activity. Antitumor activity was deter­mined using brine shrimp lethality assay while antibacterial assay was performed using turbidimetric method. Both assays utilized 96-well microtiter plates to facilitate speed and ease in screening. The chloroform extract of H. nobilis gave a positive result on antitumor activity while almost all sample extracts showed antibacterial activity against E. coli.
Vitamin D3 Adjuvant Treatment Stimulate Interleukin-10 Expression in Children with Nephrotic Syndrome Without Affecting to Clinical Outcome and Glucocorticoid Receptor Expression Husnul Asariati; Krisni Subandiyah; Loeki Enggar Fitri
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
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Abstract

Idiopathic nephrotic syndrome (INS) is the most glomerular disease that occurred in childhood with high rate morbidity. Glucocorticoid is drug of choice for INS and responsiveness to this drug determined prognosis. Glucocorticoid upregulate transcription of anti-inflammatory cytokines such as IL-4 and IL-10. IL-10 is an anti-inflammatory cytokine and has multiple role in immune response include modulate Th1/Th2 response. Vitamin D3 interact with glucocorticoid signaling. Administered active form of vitamin D3 increase dexamethasone-induced IL-10 expression by regulatory T cells in steroid resistant asthmatic patient. Here we showed increase of CD4+IL10+ expression after treatment both prednisone only and combination prednison with vitamin D3. Both in new-onset NS or rare relaps NS, combination treatment prednisone + vitamin D3 increase CD4+IL10+ expression significantly compared to prednisone-only treated group (p= 0.003), which first group (new-onset nephrotic syndrome + prednisone and vitamin D3 treatment) showed the most CD4+IL10+ expression enhancement (9.53±3.89). However this study failed to show a correlation between CD4+IL-10+ expression after prednisone and vitamin D3 treatment with clinical outcome (linear regression test, p= 0,125). This study also showed that there was a no correlation between CD4+IL-10+ expression and CD3+GR expression after prednison + vitamin D3 treatment (p= 0.088). CD4+IL-10+expression in new-onset and rarely relapsing nephrotic syndrome patients higher in prednisone + vitamin D3 treated group than prednisone-only treated group. There is no correlation between CD4+IL-10+expression and CD3+GR expression nor CD4+IL-10+expression and clinical outcome.
The Curative Activity of Isolated Fraction from Spathodea campanulata Beauv Stem Bark on Rats Exposed to Benzopyrene Masruri Masruri; Mohamad Lutfillah; Ady Sumaryanto; Rurini Retnowati; Aulanni'am Aulanni'am
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.04.03.01

Abstract

This paper reports a screening results of the secondary metabolites composed in Spathodea campanulata Beauv stem bark, evaluate inhibiting activity of malondialdehyde (MDA) on rats cancer model exposed with benzopyrene, and the histology of its lung. The secondary metabolite of the stem bark fraction consisted of alkaloids, flavonoids-phenolic, terpenoid and steroid compounds. The isolated fraction contained of these metabolites significantly indicate bioactivity by reducting of malondialdehyde (MDA) level, and also histology appearance of the lung tissue prepared from the benzopyrene-exposed rat indicated a curative activity.

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