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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 986 Documents
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Vegetation Diversity Quality in Highland Forest of Ranu Regulo Lake Area, Bromo Tengger Semeru National Park, East Java Haryati, Jehan Ramdani; Hakim, luchman
Journal of Tropical Life Science Vol 2, No 1 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

Aim of this research was to study vegetation diversity quality in highland forest of Ranu Regulo Lake area in Bromo Tengger Semeru National Park (TNBTS), East Java. Field observation was carried out by vegetation analysis using sampling plots of 25x25 m2 for trees, 5x5 m2 for poles, 1x1 m2 for ground surface plants. Community structure of each lake side was determined by calculating vegetation's density, basal area, frequency, important value and stratification of species. While vegetations diversity was estimated by taxa richness, Shannon-Wiener diversity index, and rate of endemism. Each lake side forests were compared by Morisita community similarity index. The result showed that based on existed vegetation, highland forest surrounding Ranu Regulo Lake consisted of four ecosystems, i.e. heterogenic highland forest, pine forest, acacia forest and bushes. Bushes Area has two types of population, edelweiss and Eupatorium odoratum invaded area. Vegetation diversity quality in heterogenic highland forest of Ranu Regulo TNBTS was the highest, indicated by its multi-stratification to B stratum trees of 20-30m high. Heterogenic highland forests formation was Acer laurinum and Acmena accuminatissima for trees, Chyatea for poles. Taxa richness was found 59 species and 30 families, while the others were found below 28 species and 17 families. Diversity Index of heterogenic highland forest is the highest among others for trees is 2.31 and 3.24 for poles and second in bushes (H=3.10) after edelweiss ecosystem (H=3.39). Highest rate of endemism reached 100% for trees in heterogenic highland forest, 87% for poles in edelweiss area and 89% for bushes also in heterogenic highland forest. Trees, poles and herbs most similarity community showed by pine and acacia forest. Based on those five characters, vegetation diversity quality in Ranu Regulo Lake area was medium for heterogenic highland forest and edelweiss area comparing to pine and acacia forest which had very low diversity quality. This low diversity was caused by invading species E. odoratum in density, distribution and dominance for threatening growth of poles and ground surface plants local species. Invaded ecosystem were pine forest, acacia forest and edelweiss area. It hasnt major invading in heterogenic highland forest, but it has reached the edge. Therefore, environmental policy must focus on this matter soon. Keywords: highland forest, community structure, quality diversity, Ranu Regulo
STS Marker Associated with Iron Toxicity Tolerance in Rice Chrisnawati, Lili
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.11

Abstract

Iron (Fe) toxicity is one of the limiting factors for rice growth and production in paddy fields. The use of iron tolerant varieties is one of the most efficient way to solve this problems. Identification of molecular markers linked to the trait is very important to develop marker-assisted selection (MAS) to obtain tolerant lines. The objective of this study was to identify sequence tagged sites (STS) markers associated with iron tolerance trait in double haploid rice population. Forty-five double haploid lines derived from reciprocal double crossing, i.e. IR54/Parekaligolara//Bio110/ Markuti, were phenotypically screened in high Fe wetland rice field at Taman Bogo Experimental Station, Lampung. Molecular analysis performed using STS markers. The results of the association between the genetic and phenotypic analysis showed that there were three markers, i.e. OsIRT1, OsIRT2, and OsFRO2, associated with iron tolerance trait in rice. The markers have potential as selection markers for iron toxicity tolerant lines.
A Comparison Between Hyclone And Biowest: Effects To Human MSCs Proliferation, Morphology, And Osteogenic Differentiation Saskianti, Tania; Misami, Kanawa; Kato, Yukio
Journal of Tropical Life Science Vol 3, No 1 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

Mesenchymal stem cells (MSC) are expanded in a basal culture medium supplemented with fetal bovine serum (FBS) with or without additional growth factors. The serum contains basic components such as hormones and growth factors which provide robust MSC proliferation. However, the effects of different serum in the isolation and proliferation of primary MSC remains unclear. In this study we compared effect of serums on stimulating MSC proliferation and osteogenic differentiation. Therefore we evaluated the impact of Hyc1 and Bw1 containing medium on primary MSC morphology. Primary MSC grown in both serum containing medium retain the ability to differentiate into osteoblast. Bw1 containing serum showed slightly, unsignificanty higher potential as compared to Hyc1. However more detailed analysis on the composition of each serum on overall MSC morphology and proliferation must be further explored. The result of this study should form a basis for further studies examining specific substance needed in MSC proliferation and differentiation in more detail.
Evaluation of Indigenous Potato Challisha (Solanum tuberosum L. Cv. Challisha) Somaclonals Tolerance to Salinity In Vitro Biswas, Md. Sanaullah; Islam, Md. Riazul; Zakaria, Mohammad
Journal of Tropical Life Science Vol 7, No 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.13

Abstract

Potato is one of the most important food crops in the world. It is generally sensitive to salinity and likes to grow in neutral soil. On the other hand, salinity is increasing alarmingly in the ever changing climatic conditions. Thus, the selection of salt tolerant potato cultivars is necessary to keep pace the production of potato. To select salt tolerant cultivars, here we attempt to compare the salinity level between indigenous and modern cultivars. In vitro selection of local and modern potato cultivars were investigated with five levels of NaCl (0, 30, 60, 90 and 120 mM). The indigenous potato Challisha and modern cultivars Diamant and Felsina were used as plant materials. Significant differences were noticed among the cultivars in response to different levels of NaCl. Plant growth and root development were gradually reduced with increased concentration of NaCl. All three cultivars were survived well with exhibiting different growth status up to 60 mM NaCl, but they performed poorly at 120 mM of NaCl. Cultivar Challisha performed better regarding shoot length, root length, the number of nodes per plantlet and the fresh weight per plant up to 90 mM of NaCl. Thus, we can conclude that local indigenous variety Challisha is salt tolerant comparing with the modern cultivated varieties.
Toll Like Receptor 4 (Tlr4) And p65 Nuclear Factor Kappa B (Nf-Kb) Expression In Monocyte Cell Of Children With Steroid Resistant Nephrotic Syndrome Wahyono, Tikto; Subandiyah, Krisni; Fitri, Loeki Enggar; Endharti, Agustina Tri
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

37% Phosphoric Acid Induced Stronger Matrix Metalloproteinase-8 Expression of the Dental Pulp than 19% Ethylene Diamine Tetraacetic Acid Fatimatuzzahro, Nadie; Haniastuti, Tetiana; Handajani, Juni
Journal of Tropical Life Science Vol 4, No 3 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Introduction of Pack Test for Participative Environmental Monitoring and Environmental Education for Sustainability in Malaysia Abd Rahman, Muhammad Faiz Bin
Journal of Tropical Life Science Vol 1, No 2 (2011)
Publisher : Journal of Tropical Life Science

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Abstract

Effect of Nigella Sativa Extract on Inflammatory Cells, Interleukin-10, Interferon-γ and Histological of Kidney in Monosodium Glutamate-Induced Rats Yousif, Abdalrauf A Mahmud; Sujuti, Hidayat; Widjajanto, Edi
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.04

Abstract

There is considerable evidence, suggest that, consumption of food additives monosodium glutamate (MSG), a flavor enhancer was unhealthy. Herbal medicine Nigella sativa (NS) has antioxidant properties able to cure the toxic induced by MSG. This study aimed to evaluate the risks of excessive use of MSG and to study the role of NS to inhibit inflammation and renal damage. Treated rats (twenty four male wistar rats) were divided into six group and analyzed by measuring the cells in blood, interleukin-10, interferon-γ serum levels by ELISA method and remove kidneys for histological examination. Histological of kidney for all groups except control, were showed different abnormalities include congestion of some blood vessels, hemorrhage  between tubules, widening in the renal tubules, revealed severe dilatation of Bowman's capsule and shrinkage of glomeruli, and areas of huge vacuole, were observed compared with control. Interleukin-10 was reduced in Groups 2,3,4 and 5, whereas increase in NS group compared with control. Interferon-γ was increased in groups 2,3,4 and reduced in groups 5,6 compared with control.  Eosinophil was increased in groups 2,5 and reduced in groups 3,4, 6 compared with control. This present study showed that administration of MSG to rats induced many changes effects on inflammatory cells, cytokines and histological of kidneys. NS has benefit in blood parameters, whereas harmful on kidney at these doses.
Survey on the use of synthetic Food Colors in Food Samples procured from different educational institutes of Karachi city. Saleem, Nida; Umar, Zahida Nasreen; khan, Seema Ismat
Journal of Tropical Life Science Vol 3, No 1 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

The present study was carried out to find the type of food colors added to various food products especially those vended at or near different educational institutes of Karachi city. Different types of food items were analysed for isolation and identification of the added synthetic food colors. The majority of branded food items contained permitted colors however some foods manufactured locally, contained non-permitted colors. About 11% branded and 44% unbranded food items, respectively, were found with not permitted colors for human consumption. Similarly, 4% branded and 30% unbranded beverages were found unfit due to the presence of prohibited colors. Incidences of the use of non-permitted food colors were higher in case of unorganized food makers. Constant vigilance is needed to ensure that the local manufacturers comply with the regulations of food colors not only in terms of non-permitted food colors but also about the control and limits of permitted food colors. Moreover, consumption of colored food items should also be controlled by making the society aware of the hazardous effects of food colors.
Toxicity and Lethality Evaluations of Koordersiodendron pinnatum Leaves Methanolic Extract in DDY Mice Banjarnahor, Sofna Dewita Sari; Dewijanti, Indah Dwiatmi; Angelina, Marissa; Fajriah, Sofa
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.10

Abstract

The study of toxic features of Koordersiodendron pinnatum is vital for further studies of its pharmacological activities. Acute toxicity test was done on methanolic extracts of K. pinnatum in DDY mice. Animals were grouped into five: Group 1 was given 1 mL solution of 2.5% Tween 80 in a single oral dose; the remaining groups were orally given a single dose of 2, 4, 8 and 16 g/kg of K. pinnatum, respectively. Toxic effects of the extract were evaluated on the basis of behavioral observations in the form of locomotor activity; curiosity; defecation; urination and also animal mortality. Observations were carried out for 14 days. No significant changes on body weight, and behavioural activities were recorded. Mortality was recorded up to 2% of the male group, and no mortality within the female group. The extract is practically non toxic for both male and female (LD50>15 g/kg).

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