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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 986 Documents
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Do fish have rights in artisanal fisheries? Mustapha, Moshood
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

Artisanal fishers in developing world are unaware that fish are capable of suffering or discomfort, though researches have shown that fish do feel pain. Five fish welfare domains have been identified which constitute their rights in their environment. The needs of wild fish are usually provided in their natural, undisturbed and unperturbed aquatic environment, of which the fish will prefer. However, various anthropogenic activities by humans (including artisanal fisheries itself) and some natural perturbations in the watershed, riparian zone, water body of the fish habitat and on the fish tend to take away these needs thereby compromising the fish welfare. These activities include environmental degradation, boat/canoe building, use of motorized engine boats/canoes, use of active and passive fishing gears, obnoxious cultural, religious and social fishing practices, fish harvesting, handling and processing among others. One way to understand the welfare needs of an individual fish is to understand its biology. Poor welfare conditions could then be assessed by how far the individual fish has deviated from the normal conditions. Non-intrusive signs based on the health, behavior, morphological anomalies, swimming, reduction in population and growth, outbreak of parasitic infections, injuries and loss of condition can be used to assess fish whose welfare has been compromised. Artisanal fishers should not only be concerned with catch, but, also the welfare of the fish being caught. This is because if the welfare of the fish is compromised, it is going to definitely affect the catch. As indispensable as fish is to humans, humans should not derive its pleasure at the expense of fish suffering. Human activities that impinge on the welfare of wild fish may not necessarily be stopped, but at least minimized in order to have continued sustainable artisanal exploitation of the fisheries. Keywords: welfare, artisanal fisheries, developing world, stress, behaviour, feel, habitat
Cysteine, Malondyaldehide (MDA) and Glutathione (GSH) Levels in Marasmic Type Malnutrition Cahyani, Dwi Indah; Puryatni, Anik; Permatasari, Nur
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.11

Abstract

Micronutrien deficiency in severe malnutrition will reduce antioxidant capacity that needed for oxidative stress defense. Cysteine, a non-essential amino acid, is one of an important component for reduced glutha-tione (GSH). This study aims to prove the difference between the levels of cysteine, MDA and GSH levels in children with marasmic malnutrition and well-nourished children and prove whether there is a relationship between those parameters. Fiftysix patients participated in this study were grouped into two groups of sam-ples that were marasmic type malnutrition group (28 patients) and control groups that were well nourished group (28 patients). Examination begins with a complete laboratory screening, followed by examination of cysteine, MDA and GSH level. Of the 28 patients included in marasmic type malnutrition group consisting of 15 male patients (53.6%) and 13 female patients (46.4%), while the well-nourished group consisted of 13 male patients (46.4%) and 15 female patients (53.6%). The average age is 54.61±56.35 months in the group of ma-rasmic type malnutrition and 48.25±45.34 months in the well-nourished group. By using the Mann Whitney test, there were significant difference between the levels of cysteine and GSH in marasmic malnutrition and control group (p = 0.000 and p = 0.000 respectively). Spearman correlation test between cysteine and GSH levels, cysteine and MDA levels, MDA and GSH levels in marasmic malnutrition children shows no significant correlation (R = -0.206, p = 0.294; R = -0.036, p = 0.856; R = 0.210, p = 0.284 respectively). In well-nourished group Spearman correlation test between cysteine and GSH levels, cysteine and MDA levels, MDA and GSH levels also shows no significant correlation (R = -0.053, p=0.789; R = -0.146, p = 0.458; R = -0.079, p = 0.688 re-spectively). From this study it can be concluded that there are significant differences of the levels of cysteine and GSH between severe malnutrition groups compared with the control one. But this study shows no signif-icant correlation between the levels of cysteine and GSH levels in marasmic type malnutrition and well-nourished children.
The Correlation Between IL-1β and IL-10 Levels in Estimating The Risk of Febrile Seizures Gunawan, Andi; Muid, M; Suyudi, Hidayat; wisnu, Barlianto; Subandiyah, Krisni
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Febrile seizures are the most common forms of seizures in children with fever. A data from clinical and experimental studies suggest the potential role of immune generate products in their genesis. The balance between proinflammatory (IL-1β) and anti-inflammatory (IL-10) cytokines influences the regulation of infections and plays a role in the pathogenesis of febrile seizures. In existing literature, there is no research on the correlation between IL-1β and IL-10 levels as well as the ratio of IL-1β to IL-10 in estimating the risk of febrile seizures in seizure prone children aged between 3 months and 5 years. In this study, IL-1β, IL-10 levels and ratio of IL-1β to IL-10 in the risk of febrile seizures were investigated, and respondents were divided into three groups: febrile seizures, febrile without seizures and healthy children without histories of febrile seizure. This study has used A cross-sectional design, and each group had 17 co-respondents. IL-1β and IL-10 levels were measured by ELISA. Data were analyzed by SPSS 15. Findings showed there were significant differences between IL-1β and IL-10 as well as in the ratio of IL-1β to IL-10 between febrile seizure patients and healthy children. It was concluded that there were significant correlations between IL-1β and IL-10 levels as well as IL-1β to IL-10 ratios in estimating the risk of febrile seizures.
Isolation of an Anti-Cancer Asperuloside from Hedyotis corymbosa L. Artanti, Nina; Hanafi, Muhammad; Andriyani, Rina; Saraswaty, Vienna; Udin, Linar Zalinar; Lotulung, Puspa D; Fujita, Ken Ichi; Usuki, Yoshinosuke
Journal of Tropical Life Science Vol 5, No 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.02.06

Abstract

Hedyotis corymbosa, with local name rumput mutiara, is an anti-inflammatory, anti-cancer and hepatoprotective traditional medicine.  The ethanol extract of H. corymbosa L. shows inhibitory activity to human YMB-1 breast cancer cell line with an IC50 of 6.51 mg/ml.  The methylene chloride fraction shows a potential cytotoxic activity with an IC50 of 2.75 mg/ml.  To obtain a lead compound, the extract was further purified by column chromatography. A pure compound is obtained which shows inhibitory activities against YMB-1, HL60 and KB human cell lines with IC50 values of 0.7; 11.0 and 104.2 mg/ml, respectively.  Based on the 1D and 2D FT-NMR data, the isolated compound is an asperuloside.
The Growth of Lactobacillus fermentum Isolated from Quail Intestine on Rice Bran Medium Kalsum, Umi; Sjofjan, O.
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

The objective of this study was to investigate the growth of Lactobacillus fermentum isolated from the intestine of Japanese quail (Coturnix japonica) on medium variation of rice bran meal. The influence of this medium preparation on the cell number and produce of lactic acid of L.fermentum was evaluated every six hour in 36-hour incubation. A randomized block design was used to assign three variation of rice bran medium (6, 9 and 12) %. The result showed that the higest cell number was in 9 % rice bran medium at pH 4.7 at 12th hour was 5.2 x 1010 CFU/ml and the highest lactic acid concentrations were 0.57 %. The fastest speed of growth in 9 % rice bran medium was 0.92 generation/hour and generation time 45 minutes. In conclusion, the optimum growth of L.fermentum was in 9 % rice bran medium at 12th hour.Keywords: Lactobacillus fermentum, growth, quail, rice bran.
PCR-RFLP and Sequencing of trnS/trnfM Fragment of Enhalus acoriodes from Sanur Coastal Waters, Bali, Indonesia: A Preliminary Study Pharmawati, Made; Imaniar, Eka Fibayani
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.10

Abstract

This study aimed to evaluate the resolution of PCR-RFLP and sequencing of trnS/trnfM in detecting genetic diversity of seagrass Enhalus acoroides at Sanur Beach, Sindhu Beach and Semawang Beach. This research used six samples E. acoroides from each location. DNA extraction method followed Doyle and Doyle. PCR amplifications were done using primers P672/P673, P674/P675 and ITS4/ITS5 followed by digestion using restriction enzymes EcoRI, PstI, HindIII, BamHI, Rsa, Mva and HinfI. Sequencing analysis used PCR products of primers trnS/trnfM. Testing of DNA sequences of E. acoroides were conducted using BLAST (Basic Local Alignment Search Tool). DNA sequences were further analyzed using MEGA 5.2 software (Molecular Evolutionary Genetic Analysis) to evaluate variations of DNA. The sequence alignments were done using ClustalW software to determine the homology between the DNA sequences. The results showed that 18 samples of E. acoroides from Sanur, Sindhu and Semawang Beach have no polymorphism based on restriction enzyme analyses. Furthermore, sequencing of trnS/trnfM region of 18 samples E. acoroides showed that the sequences were identical.   
Ethnobotanical Investigation for Conserving Home Gardens Plants Species in Tambakrejo Sumbermanjing Wetan, Southern of Malang Pamungkas, Ratih Nila; Hakim, Luchman
Journal of Tropical Life Science Vol 3, No 2 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

In developed countries, traditional knowledge of indigenous people is being widely threatened by current trends of economic globalization especially in destination tourism area such as Tambakrejo village, Sumbermanjing Wetan, Malang. This region includes Sempu Island as well as proposed protected area. Investigation aims were to know diversity of plants species that arranged home garden, used value of each plant species using ethnobotany indexes, and also to describe their methods of preparation and use of the significant plant. The study represents the first step to documenting significant utilitized of the species plant in home garden based on indigenous knowledge before it disappears. Data were collected by depth and semi structured interviews. Informations were about traditional uses of wild plants also cultivated ones. All the collected data were filled in a analytical table and for easier, data was grouped into ten categories, then continued with detailed of use. Data was calculated by using synthetic indexes, those are Relative Frequency of Citation (RFC) and Cultural Importance Index (CI). One hundred and one plants were cited by the informants as being traditionally used in the area. These 100 ethnospecies belonging to 45 families. From 10 categories, most of them concerning allimentary, medical and economic plants. For the allimentary plants, 316 citation, 58 species and 5 different uses were recorded. While for the medical plants, there were 63 citations, 22 species and 4 different uses categories. A few plants species for others categories like erotion control. The study shows that traditional uses for plants especially at home garden still survives as part of the cultural heritage of the community, economic and also for daily activity. Keywords:Ethnobotanical, Home garden, Traditional knowledge
Microhabitat Assessment of Mangrove Pit Viper Cryptelytrops purpureomaculatus in Restricted Mangrove Area, Dumai, Riau Province, Sumatera Kurniawan, Nia; Priambodo, Bagus; Nugraha, Fitra Arya Dwi; Maulidi, Andri; Kurnianto, Agung Sih
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.01

Abstract

Bandar Bakau is a mangrove conservation area with approximately 20 ha that preserves many kinds of fauna, including Mangrove Pit Viper Cryptelytrops purpureomaculatus. However, this species and its habitat are still poorly studied in Indonesia, particularly Sumatera island, despite it has a wide distribution in Southeast Asian countries. The main purpose of this study is to evaluate the microhabitat of Mangrove Pit Viper C. purpureomaculatus that found at a restricted mangrove area in Dumai, Riau Province. Visual Encounter Survey (VES) and deep interview methods were used in this study. The survey found six individuals of C. purpureomaculatus that mostly basking on the Avicennia marina and artificial building. Although Bandar Bakau is a tourism object, but no snakebite cases were found.
Dynamics of a Fractional Order Eco-Epidemiological Model Nugraheni, Kartika; Trisilowati, Trisilowati; Suryanto, Agus
Journal of Tropical Life Science Vol 7, No 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.09

Abstract

In this paper, we propose a fractional order eco-epidemiological model. We considere the existence of time memory in the growth rate of the three populations. We observed the dynamical behaviour by analysing with fractional order and then simulateing using Grünwald-Letnikov approximation to support analytical results. It found that the model has five equilibrium points, namely the origin, the survival of susceptible prey, the predator free equilibria, the infected prey free equilibria, the interior equilibria. Numerical simulations show that the existence of fractional order  is a factor which affects the behaviour of solutions. 
Diabetic Blood Glucose Level Profile with Stratified Dose Streptozotocin (SD-STZ) and Multi Low Dose Streptozotocin (MLD-STZ) Induction Methods Hikmah, Nuzulul; Shita, Amandia Dewi Permana; Maulana, Hafiedz
Journal of Tropical Life Science Vol 5, No 1 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.5.1.%x

Abstract

The prevalence of diabetes increased from year to year. Diabetic animal model was developed to study the pathogenesis and treatment in diabetes. The aim of this study was to determine the profile of diabetic blood glucose levels using a stratified dose streptozotocin (STZ-SD) and multi-low dose streptozotocin (MLD-STZ) induction methods. 27 Wistar rats were divided into three groups, control group, SD-STZ group, and MLD-STZ group. The induction of experimental animal was using the method of SD-STZ and MLD-STZ for 5 consecutive days intraperitoneally. SD-STZ method using stratified dose of 40, 35, 30, 25, and 20 mg/Kg of body weight, while MLD-STZ method using a dose of 20 mg/Kg of body weight for 5 times. The measurement of blood glucose level (BGL) and body weight (BW) in animal was made on 2, 4, 7, and 14 days after STZ induction. The results of this study indicate that the diabetic conditions (BGL ≥ 300 mg/dl) on the SD-STZ induction method was more stable, and no deaths occurred in experimental animals.Keywords: Streptozotocin (STZ), diabetes, blood glucose level (BGL).

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