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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 986 Documents
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The assessment of land exploitation by enumerating microbial population: Case study in several locations at Dieng Plateau Sumarsih, Sri; Virgawati, Sari
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

Agricultural intensification program in Indonesia which is carried out by using high yield variety, high chemicals use and soil disturbances tends to trigger land exploitation. Land exploitation, performed without considering the lands capability can generate degradations on the land itself. Various methods have been used to determine land exploitation level, including evaluation of soil microbe resources as on soil component. This research is aimed to assess land exploitation level, based on the amount of microbial population. The result of this research is expected to add the soil quality standard criteria. In the case study performed in Dieng plateau, representative soil sampling method was used. The amount of microbial population can be enumerated using plating and MPN method. Based on nutrient availability to indicate the soil biological characteristics, the soil under the trees, shrub, and Colocasia were classified as “below normal”, and the soil under the grass, tobacco, cabbage and potato were classified as normal. It shows that the land exploitation at the agricultural soils were still in the range of its land capability.
Growth and Development of Apogamous Adiantum lunulatum Burm. f. Gametophyte from Dry and Humid Areas in Java Island Novitasari, Risti; Chikmawati, Tatik; Sulistijorini, Sulistijorini
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.15

Abstract

Adiantum lunulatum Burm. f. has been widely used in aesthetic needs and medical fields. Detail information about the life cycle of A. lunulatum is still unknown, particularly about its gametophyte generation. Present work aimed to study and compare the growth and development of the gametophytes of A. lunulatum from dry and humid areas. Research method consists of two stages: (1) Spores were collected from two locations, Pasuruan and Bogor and (2) Spores were planted and observed the growth and development of gametophyte. The result showed that natural habitat of the plant in various dry and humid areas, affecting the rate of growth and development rates of A. lunulatum gametophyte. The gametophyte from the dry area showed faster rate of growth and development than that of humid areas. The spores collected from dry area need 9 weeks for germination, growth, and development while the spores of humid area took 22 weeks.
Assessment of vegetative Phenology with respect to leaf elongation pattern of Avicennia marina and Rhizophora mucronata in Hajambro creek, Indus delta, Pakistan Farooqui, Zafar; Siddiqui, Pirzada Jamal
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Leaves emergence and the rate of elongation have been studied in Hajambro creek in Indus deltaic region, Pakistan. Leaves emergence was found higher during the summer months (mostly pre-monsoon period), that was March April, May and June. Leaves of A. marina that were observed from March to July, till the leaves reached their maximum length (about 77 mm), showed a gradual increase in their length, reaching maximum in April. Leaves of Avicennia marina reached their maximum length (about 77 mm) in about 113 days. The leaves showed gradual increase in their length, reaching maximum in April (0.97mm d-1). The per-day increment in length was 0.54 mm d-1. As reaching their maximum length (77mm), the per-day increment in the length gradually slower down. The leaves of Rhizophora mucronata were observed from February to November. The leaves of Rhizophora mucronata showed elongation rate of 0.24 mm d-1 from the day of the first observation till the leaves reached their maximum length (120 mm). The maximum time required by the leaves to reach their maximum length (120 mm) was 236 days. The rates of elongation of leaves are also fast in summer. The rate of elongation of the leaves was found to be highly correlated with the temperature (p
Significance of the Easy-to-use Water Quality Checker for Participative Environmental Monitoring and Experience Based Learning Kikuchi, Akira; Hakim, Luchman; Heryansyah, Arien; Romaidi, Romaidi
Journal of Tropical Life Science Vol 1, No 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Pack Test is a series of products of Kyoritsu Chemical-Check Laboratory Cooperation, Japan. It is easy-to-use, anybody can use at anywhere, low cost, nontoxic, safe, and professional-use, onetime use ion-selective color metric water quality checker. The aim of this research is to assess, then next, discuss the significance of applications of this tool. In this order, NH4-Pack Test was selected as an example for the discussions; although there are more than 60 parameters can be detected by pack test such as, COD, Cl-, NO3-, phosphate, hardness, pH, heavy metals, etc.. As for field survey, Ion chromatography was used to measure ammonium concentration of river water in Jakarta. Then detection range of the NH4-Pack Test was compared to the data, and Indonesian and Malaysian national water quality standard. River water quality of Jakarta was weekly degraded at upstream area, but it was seriously degraded at downstream area (up to 5-10 NH4-N mg/L). As for ammonium concentration, obviously the detection range and step of Pack Test was sufficient to assess the ammonium concentrations of rive water in Jakarta. Of course Pack Test is very simplified tool, environmental water quality standard of ammonia for class I in Indonesia and Class I and II in Malaysia were difficult to evaluate. However, it was obviously applicable to check treated effluent and Class III to V water quality of Malaysian environmental standard. Consequently, it is suggested to adopt a double standard policy of water quality monitoring, such as combination of "easy-to-use simplified" and "conventional-accurate". Because of low cost, and professional-convenient design, implementation of Pack Test will significant to empower on-site water quality monitoring in developing country, participative environmental awareness public programs, experience base environmental learning in schools, and other grass-rooted environmental activities.Keywords: Environmental awareness and education, Pack test, Simplified method, Southeastern Asian county
The effect of Nigella Sativa extract on Alpha-ketoglutarate activity and histopathologic changes on Rat liver induced by Monosodium glutamate Eshami, Ala Shukri; mitaroem, Karyono; Sujuti, Hidayat; Ashour, Abdussalam
Journal of Tropical Life Science Vol 5, No 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.03

Abstract

         Monosodium glutamate (MSG) is a commonly used food additive and found in most soups, fish and processed meat. The use of MSG in food is growing. Irrational fear had increased in the last few years due to the adverse reactions and toxicity of MSG, which effect on the liver.  Nigella sativa is used as traditional medicine for the treatment of many diseases, it has been extensively investigated in recent years, traditional medicine for the treatment of many disease due to its notable pharmacological properties. NS can inhibit oxidative stress. The present study was undertaken to investigate different Doses of Nigella sativa on alpha KGDH activity and liver histology of MSG induced rat. The animals (n=30) Were grouped  A as (control), B treated with MSG 1g/kg, C MSG treated with NS 0.01g/kg, D MSG treated with NS 0.02g/kg, E MSG treated with NS 0.04g/kg and F treated with NS 0.02g/kg then we investigated the effect alpha KGDH activity by ELISA method and liver histopathology by light microscope.  alpha KGDH activity were significantly increased in treatment MSG compared with treatment negative control, MSG + NS 0.1g/kg, MSG + NS 0.02g/kg, MSG + NS 0.04g/kg, and NS 0.02g/kg. The histological changes in group B and C showed disturbed liver architecture, hemorrhage in the central veins, areas of necrosis, vacuolation and increased inflammatory cells infiltration while group D showed central vein is normal and vacuolation of cytoplasm, group E showed more normal liver architecture as shown less vacuolation in cytoplasm, central vein is normal, and group F showed no pathologic changes.These finding showed that administration of MSG increase alpha KGDH and induced damage in liver tissue. Nigella sativa extract can reduce alpha KGDH and prevent liver damage induced by MSG.Keywords :  Monosodium glutamate, Alpha-ketoglutarate Dehydrogenases, Nigella sativa and liver damage.
Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara Adriaty, Dinar; Wahyuni, Ratna; Iswahyudi, Iswahyudi; Aksono, Bimo; Koesbardiati, Toetik; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.2.3.%x

Abstract

The existence of leprosy or kusta or Morbus Hansen or Hansens disease has been known for years, including in Indonesia. Starting from the discovery of Mycobacterium leprae isolates from ancient bone (about 1.000 years B.C), the archaeological excavations results in East Nusa Tenggara, interesting questions arise about how the development of leprosy in eastern Indonesia is. Biology molecular study would become a powerful tool to investigate the presence of leprosy bacillary whether there are similarities between the genomes of M. leprae isolates in the primeval and the present. PCR examinations were performed on mandibular bone fragments from ancient human who lived 1000 years B.C. discovered in archaeological surveys on the island of Lembata and three leprosy patients from East Nusa Tenggara. The DNA extraction was performed using a kit from Qiagen products and its TTC repeating pattern was seen with the method of direct sequencing. It turned out that the TTC profile obtained from samples of archaeological was as many as 13 copies, while the repetition of TTC in three samples of leprosy patients were 15, 17 and 26 copies. The different number of TTC repetition shows the different isolates of M. leprae between in the ancient times and the present. Further studies are needed to verify the differences in the genome that occur, for example from the study of SNPs (single nucleotide polymorphisms).
Baculovirus Surface Display Using Infuenza Neuraminidase (NA) Transmembrane Anchor Trianti, Irisa; Akeprathumchai, Saengchai; Mekvichitsaeng, Phenjun; Poomputsa, Kanokwan
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.12

Abstract

Baculovirus surface display has been employed as an excellent tools for presentation of foreign peptides and proteins on virus surface with native conformation, functions and immunogenicity. A baculovirus major envelope protein, gp64, or a capsid protein, vp39 are generally used as fusion partners for displaying of polypeptides on the surface of virions. Alternatively, a membrane anchoring domain of vesicular stomatitis virus G protein (VSV-G) can also be used. In this study, an influenza neuraminidase (NA) was proposed as a new membrane anchor for the display of Angiotensin II (AngII), DRVYIHPFHL, peptides. The AngII peptides were inserted into NA by replacing NA amino acid number 60-67 with AngII, and then integrated into a baculovirus genome. A recombinant baculovirus expressing the NA fusion-AngII peptides was generated from infected insect cells. Those peptides were found to express and translocated on the membrane of the baculovirus infected insect cell (Sf9 cell) as detected by immunocytochemistry using anti-AngII monoclonal antibody. Upon budding of the recombinant baculovirus progenies through the insect cells membrane, the recombinant NA-AngII peptides was acquired to envelopes of the new baculovirus progenies. The conformation of NA on baculovirus surface was not affected by the deletion, as the 55 kDa band of NA can be detected from Western Blotting analysis by specific anti-NA monoclonal antibody. In addition, the same protein was also found by anti-AngII antibody indicating that the AngII peptides had been successfully fused with the recombinant NA. Interestingly, electron microscopy analysis demonstrated that not only the recombinant baculovirus displaying AngII peptides were generated by infected insect cells, but also the NA virus-like-particle displaying AngII peptides.
The Effect of Calcusol™ to the Plasma Free Radical and Serum Creatinin in Mus Musculus Nephrolithiasis Model Rosyidah, A’liyatur; Widyarti, Sri; Rahayu, Sri
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.%x.%x.%x

Abstract

In Silico Screening and Designing Synthesis of Cinchona Alkaloids Derivatives as Potential Anticancer Hanafi, Muhammad; Rosmalena, Rosmalena; Prasasty, Vivitri Dewi; Udin, Linar Zalinar; Primahana, Gian
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.06

Abstract

P-glycoprotein (P-gp) resistance in cancer cells decreases intracellular accumulation of various anticancer drugs. This multidrug resistance (MDR) protein can be modulated by a number of non-cytotoxic drugs. We have screened 30 chincona alkaloids derivatives as a potent P-gp inhibitor agent in silico. Hereby, we report the highest potential inhibitions of P-gp is Cinchonidine isobutanoate through molecular docking approach. with affinity energy -8.6 kcal/mol and inhibition constant, Ki is 4.89 x 10-7 M. Cinchonidine isobutanoate is also known has molecular weight below 500, Log P value 3.5, which is indicated violation free of Lipinski`s rule of five. Thus, Cinchonidine isobutanoate is the most potent compound as anticancer compare to other Cinchona alkaloids. Ultimately, we design Cinchonidine isobutanoate for further lead synthesis by using DBSA, act as a combined Brønsted acid-surfactant-catalyst (BASC) to obtain high concentration of organic product by forming micellar aggregates which is very powerful catalytic application in water environment.
Ecotourism and Climates changes: the ecolodge contribution in global warming mitigation Hakim, Luchman; Nakagoshi, Nobukazu
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Global attention to the global warming reduction has invite numerous strategy implemented with the objectives is mitigating greenhouse gasses emission which threats to the future of living in biosphere. Essentially, absorbing CO2 from atmosphere and sequestering in terrestrial ecosystem is one of the significant strategy. While in developing countries it is become essential, support for forest conservation, afforestation and effort to increase terrestrial ability to capture and storage carbon is poor. Ecotourism offer potential key to solved such problems by promoting ecolodge as a sustainable tourism accomodations. This paper aims to explore the potential of ecotourism sector to alleviate global warming and establishing framework for ecolodge planning and development in tropical developing countries. This paper highlight the significant of ecolodge attraction and development management to meet proper carbon capture and sequestration mechanism. The attraction management and developing programs ultimately able to increase plants biomass while accommodation able to practicing energy efficient and optimizing reuse and recycle approach. It will become the potential solution for reducing greenhouse gas emissions and create clean development strategy.

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